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Protein

Archaeal Lon protease

Gene

PH0452

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Degrades polypeptides processively (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei990By similarity1
Active sitei1033By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi59 – 66ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease, Protease, Serine protease

Keywords - Biological processi

Intron homing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-450-MONOMER.

Protein family/group databases

MEROPSiS16.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Archaeal Lon protease (EC:3.4.21.-)
Alternative name(s):
ATP-dependent protease La homolog
Cleaved into the following chain:
Gene namesi
Ordered Locus Names:PH0452
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 124CytoplasmicSequence analysisAdd BLAST124
Transmembranei125 – 145HelicalSequence analysisAdd BLAST21
Transmembranei146 – 166HelicalSequence analysisAdd BLAST21
Topological domaini167 – 1127ExtracellularSequence analysisAdd BLAST961

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000267291 – 210Archaeal Lon protease, 1st partSequence analysisAdd BLAST210
ChainiPRO_0000026730211 – 684Pho lon inteinSequence analysisAdd BLAST474
ChainiPRO_0000026731685 – 1127Archaeal Lon protease, 2nd partSequence analysisAdd BLAST443

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Subunit structurei

Homohexamer. Organized in a ring with a central cavity (By similarity).By similarity

Protein-protein interaction databases

STRINGi70601.PH0452.

Structurei

3D structure databases

ProteinModelPortaliO58221.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini417 – 572DOD-type homing endonucleasePROSITE-ProRule annotationAdd BLAST156
Domaini904 – 1083Lon proteolyticPROSITE-ProRule annotationAdd BLAST180

Sequence similaritiesi

Contains 1 DOD-type homing endonuclease domain.PROSITE-ProRule annotation
Contains 1 Lon proteolytic domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiarCOG02160. Archaea.
arCOG03158. Archaea.
ENOG4102TCU. Archaea.
COG1067. LUCA.
COG1372. LUCA.
HOGENOMiHOG000113346.
KOiK04076.
OMAiDPFQCFS.

Family and domain databases

Gene3Di2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.30.230.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR030934. Intein_C.
IPR004042. Intein_endonuc.
IPR006141. Intein_N.
IPR004663. Lon_arc.
IPR027065. Lon_Prtase.
IPR000523. Mg_chelatse_chII_dom.
IPR027417. P-loop_NTPase.
IPR008269. Pept_S16_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR002078. Sigma_54_int.
[Graphical view]
PANTHERiPTHR10046. PTHR10046. 2 hits.
PfamiPF05362. Lon_C. 1 hit.
PF01078. Mg_chelatase. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 2 hits.
SSF54211. SSF54211. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR01445. intein_Nterm. 1 hit.
TIGR00764. lon_rel. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS51786. LON_PROTEOLYTIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O58221-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSGESEMDE ERMDLGIEFE TTEEIPVPER LIDQVIGQDH AVEVIKTAAK
60 70 80 90 100
QRRHVLLIGE PGTGKSMLGQ AMAELLPTEE LEDILVFPNP EDENMPRIKT
110 120 130 140 150
VPAGQGRKIV EEYRRKAKEQ EGVRFYLLFF VFFIVAMAVF LSHGDPNTLL
160 170 180 190 200
LGVFVILVAL MVTANMRFKT QAMVPKLLVD NSGRKRAPFV DATGAHAGAL
210 220 230 240 250
LGDVRHDPFQ CFSGEEVIIV EKGKDRKVVK LREFVEDALK EPSGEGMDGD
260 270 280 290 300
IKVTYKDLRG EDVRILTKDG FVKLLYVNKR EGKQKLRKIV NLDKDYWLAV
310 320 330 340 350
TPDHKVFTSE GLKEAGEITE KDEIIRVPLV ILDGPKIAST YGEDGKFDDY
360 370 380 390 400
IRWKKYYEKT GNGYKRAAKE LNIKESTLRW WTQGAKPNSL KMIEELEKLN
410 420 430 440 450
LLPLTSEDSR LEKVAIILGA LFSDGNIDRN FNTLSFISSE RKAIERFVET
460 470 480 490 500
LKELFGEFNY EIRDNHESLG KSILFRTWDR RIIRFFVALG APVGNKTKVK
510 520 530 540 550
LELPWWIKLK PSLFLAFMDG LYSGDGSVPR FARYEEGIKF NGTFEIAQLT
560 570 580 590 600
DDVEKKLPFF EEIAWYLSFF GIKAKVRVDK TGDKYKVRLI FSQSIDNVLN
610 620 630 640 650
FLEFIPISLS PAKREKFLRE VESYLAAVPE SSLAGRIEEL REHFNRIKKG
660 670 680 690 700
ERRSFIETWE VVNVTYNVTT ETGNLLANGL FVKNSGGLGT PAHLRVEPGM
710 720 730 740 750
IHRAHKGVLF IDEIATLSLK MQQSLLTAMQ EKKFPITGQS ELSSGAMVRT
760 770 780 790 800
EPVPCDFILV AAGNLDTIEK MHPALRSRIR GYGYEVYMRT TMPDTPENRR
810 820 830 840 850
KLVQFVAQEV KKDGRIPHFT RDAVEEIVRE AQRRAGRKGH LTLRLRDLGG
860 870 880 890 900
VVRAAGDIAV RKGKKYVTRE DVLEALKLAK PLEKQLADWY IERKKEYQVI
910 920 930 940 950
RVEGGEIGRV NGLAIIGEQS GIVLPIEAIV APAASKEEGK IIVTGKLGEI
960 970 980 990 1000
AKEAVLNVSA IIKRYKGEDI SKYDIHVQFL QTYEGVEGDS ASISVATAVI
1010 1020 1030 1040 1050
SALEEIPVRQ DVAMTGSLSV RGEVLPVGGV TPKIEAAIEA GIKTVIIPKS
1060 1070 1080 1090 1100
NEKDVFLSPD KRKKIKIIPV ERIDEVLEVA LVESEKKREL IKRVRESLPL
1110 1120
WMEETPSGET LHEHKGGATL PLEESKA
Length:1,127
Mass (Da):126,992
Last modified:January 1, 1999 - v2
Checksum:iD756DD47332891E1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29538.1.
PIRiE71156.
RefSeqiWP_010884561.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA29538; BAA29538; BAA29538.
GeneIDi1444348.
KEGGipho:PH0452.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29538.1.
PIRiE71156.
RefSeqiWP_010884561.1. NC_000961.1.

3D structure databases

ProteinModelPortaliO58221.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH0452.

Protein family/group databases

MEROPSiS16.005.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA29538; BAA29538; BAA29538.
GeneIDi1444348.
KEGGipho:PH0452.

Phylogenomic databases

eggNOGiarCOG02160. Archaea.
arCOG03158. Archaea.
ENOG4102TCU. Archaea.
COG1067. LUCA.
COG1372. LUCA.
HOGENOMiHOG000113346.
KOiK04076.
OMAiDPFQCFS.

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-450-MONOMER.

Family and domain databases

Gene3Di2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.30.230.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR030934. Intein_C.
IPR004042. Intein_endonuc.
IPR006141. Intein_N.
IPR004663. Lon_arc.
IPR027065. Lon_Prtase.
IPR000523. Mg_chelatse_chII_dom.
IPR027417. P-loop_NTPase.
IPR008269. Pept_S16_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR002078. Sigma_54_int.
[Graphical view]
PANTHERiPTHR10046. PTHR10046. 2 hits.
PfamiPF05362. Lon_C. 1 hit.
PF01078. Mg_chelatase. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 2 hits.
SSF54211. SSF54211. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR01445. intein_Nterm. 1 hit.
TIGR00764. lon_rel. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS51786. LON_PROTEOLYTIC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLONB_PYRHO
AccessioniPrimary (citable) accession number: O58221
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: January 1, 1999
Last modified: October 5, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.