O58221 (LONB_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Archaeal Lon protease
EC=3.4.21.-

Alternative name(s):
ATP-dependent protease La homolog

Cleaved into the following chain:

1. Pho lon intein

Gene names

Ordered Locus Names:

PH0452

Organism

Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]

Taxonomic identifier

70601 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus › Pyrococcus horikoshii

Protein attributes

Sequence length	1127 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Degrades polypeptides processively By similarity.
Subunit structure	Homohexamer. Organized in a ring with a central cavity By similarity.
Subcellular location	Cell membrane By similarity; Multi-pass membrane protein Potential.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.
Sequence similarities	Belongs to the peptidase S16 family. Archaeal LonB subfamily. Contains 1 DOD-type homing endonuclease domain.

Ontologies

Keywords
Biological process	Intron homing
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Endonuclease Hydrolase Nuclease Protease Serine protease
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: InterPro intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro intron homing Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC photosynthesis Inferred from electronic annotation. Source: InterPro protein catabolic process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent peptidase activity Inferred from electronic annotation. Source: InterPro endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium chelatase activity Inferred from electronic annotation. Source: InterPro serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 210	210	Archaeal Lon protease, 1st part Potential		PRO_0000026729
	Chain	211 – 684	474	Pho lon intein Potential		PRO_0000026730
	Chain	685 – 1127	443	Archaeal Lon protease, 2nd part Potential		PRO_0000026731
Regions
	Topological domain	1 – 124	124	Cytoplasmic Potential
	Transmembrane	125 – 145	21	Helical; Potential
	Transmembrane	146 – 166	21	Helical; Potential
	Topological domain	167 – 1127	961	Extracellular Potential
	Domain	417 – 572	156	DOD-type homing endonuclease
	Nucleotide binding	59 – 66	8	ATP Potential
Sites
	Active site	990	1	By similarity
	Active site	1033	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O58221 [UniParc]. Last modified January 1, 1999. Version 2. Checksum: D756DD47332891E1	FASTA	1,127	126,992
	10 20 30 40 50 60 MLSGESEMDE ERMDLGIEFE TTEEIPVPER LIDQVIGQDH AVEVIKTAAK QRRHVLLIGE 70 80 90 100 110 120 PGTGKSMLGQ AMAELLPTEE LEDILVFPNP EDENMPRIKT VPAGQGRKIV EEYRRKAKEQ 130 140 150 160 170 180 EGVRFYLLFF VFFIVAMAVF LSHGDPNTLL LGVFVILVAL MVTANMRFKT QAMVPKLLVD 190 200 210 220 230 240 NSGRKRAPFV DATGAHAGAL LGDVRHDPFQ CFSGEEVIIV EKGKDRKVVK LREFVEDALK 250 260 270 280 290 300 EPSGEGMDGD IKVTYKDLRG EDVRILTKDG FVKLLYVNKR EGKQKLRKIV NLDKDYWLAV 310 320 330 340 350 360 TPDHKVFTSE GLKEAGEITE KDEIIRVPLV ILDGPKIAST YGEDGKFDDY IRWKKYYEKT 370 380 390 400 410 420 GNGYKRAAKE LNIKESTLRW WTQGAKPNSL KMIEELEKLN LLPLTSEDSR LEKVAIILGA 430 440 450 460 470 480 LFSDGNIDRN FNTLSFISSE RKAIERFVET LKELFGEFNY EIRDNHESLG KSILFRTWDR 490 500 510 520 530 540 RIIRFFVALG APVGNKTKVK LELPWWIKLK PSLFLAFMDG LYSGDGSVPR FARYEEGIKF 550 560 570 580 590 600 NGTFEIAQLT DDVEKKLPFF EEIAWYLSFF GIKAKVRVDK TGDKYKVRLI FSQSIDNVLN 610 620 630 640 650 660 FLEFIPISLS PAKREKFLRE VESYLAAVPE SSLAGRIEEL REHFNRIKKG ERRSFIETWE 670 680 690 700 710 720 VVNVTYNVTT ETGNLLANGL FVKNSGGLGT PAHLRVEPGM IHRAHKGVLF IDEIATLSLK 730 740 750 760 770 780 MQQSLLTAMQ EKKFPITGQS ELSSGAMVRT EPVPCDFILV AAGNLDTIEK MHPALRSRIR 790 800 810 820 830 840 GYGYEVYMRT TMPDTPENRR KLVQFVAQEV KKDGRIPHFT RDAVEEIVRE AQRRAGRKGH 850 860 870 880 890 900 LTLRLRDLGG VVRAAGDIAV RKGKKYVTRE DVLEALKLAK PLEKQLADWY IERKKEYQVI 910 920 930 940 950 960 RVEGGEIGRV NGLAIIGEQS GIVLPIEAIV APAASKEEGK IIVTGKLGEI AKEAVLNVSA 970 980 990 1000 1010 1020 IIKRYKGEDI SKYDIHVQFL QTYEGVEGDS ASISVATAVI SALEEIPVRQ DVAMTGSLSV 1030 1040 1050 1060 1070 1080 RGEVLPVGGV TPKIEAAIEA GIKTVIIPKS NEKDVFLSPD KRKKIKIIPV ERIDEVLEVA 1090 1100 1110 1120 LVESEKKREL IKRVRESLPL WMEETPSGET LHEHKGGATL PLEESKA

References

[1]

"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3." Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H. DNA Res. 5:55-76(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000001 Genomic DNA. Translation: BAA29538.1.
PIR	E71156.
RefSeq	NP_142432.1. NC_000961.1.
3D structure databases
ProteinModelPortal	O58221.
SMR	O58221. Positions 897-1096.
ModBase	Search...
Protein-protein interaction databases
STRING	70601.PH0452.
Protein family/group databases
MEROPS	S16.005.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAA29538; BAA29538; BAA29538.
GeneID	1444348.
KEGG	pho:PH0452.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1067.
HOGENOM	HOG000113346.
KO	K04076.
OMA	GLFVKNS.
ProtClustDB	CLSK379941.
Enzyme and pathway databases
BioCyc	PHOR70601:GJWR-450-MONOMER.
Family and domain databases
Gene3D	3.10.28.10. 1 hit. 3.30.230.10. 1 hit.
InterPro	IPR003586. Hint_dom_C. IPR003587. Hint_dom_N. IPR027434. Homing_endonucl. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splice_site. IPR004663. Lon_arc. IPR027065. Lon_Prtase. IPR000523. Mg_chelatse_chII. IPR027417. P-loop_NTPase. IPR008269. Pept_S16_C. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR002078. Sigma_54_int. [Graphical view]
PANTHER	PTHR10046. PTHR10046. 1 hit.
Pfam	PF05362. Lon_C. 1 hit. PF01078. Mg_chelatase. 1 hit. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF52540. SSF52540. 1 hit. SSF55608. SSF55608. 1 hit.
TIGRFAMs	TIGR01445. intein_Nterm. 1 hit. TIGR00764. lon_rel. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. PS01046. LON_SER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LONB_PYRHO

Accession

Primary (citable) accession number: O58221

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	January 1, 1999
Last modified:	May 29, 2013
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program