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O58221

- LONB_PYRHO

UniProt

O58221 - LONB_PYRHO

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Protein

Archaeal Lon protease

Gene
PH0452
Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Degrades polypeptides processively By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei990 – 9901 By similarity
Active sitei1033 – 10331 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi59 – 668ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATP-dependent peptidase activity Source: InterPro
  3. endonuclease activity Source: UniProtKB-KW
  4. magnesium chelatase activity Source: InterPro
  5. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. chlorophyll biosynthetic process Source: InterPro
  2. intein-mediated protein splicing Source: InterPro
  3. intron homing Source: UniProtKB-KW
  4. photosynthesis Source: InterPro
  5. protein catabolic process Source: InterPro
  6. regulation of transcription, DNA-templated Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease, Protease, Serine protease

Keywords - Biological processi

Intron homing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-450-MONOMER.

Protein family/group databases

MEROPSiS16.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Archaeal Lon protease (EC:3.4.21.-)
Alternative name(s):
ATP-dependent protease La homolog
Cleaved into the following chain:
Gene namesi
Ordered Locus Names:PH0452
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000000752: Chromosome

Subcellular locationi

Cell membrane By similarity; Multi-pass membrane protein Reviewed prediction

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 124124Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei125 – 14521Helical; Reviewed predictionAdd
BLAST
Transmembranei146 – 16621Helical; Reviewed predictionAdd
BLAST
Topological domaini167 – 1127961Extracellular Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 210210Archaeal Lon protease, 1st part Reviewed predictionPRO_0000026729Add
BLAST
Chaini211 – 684474Pho lon intein Reviewed predictionPRO_0000026730Add
BLAST
Chaini685 – 1127443Archaeal Lon protease, 2nd part Reviewed predictionPRO_0000026731Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Reviewed prediction.

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Subunit structurei

Homohexamer. Organized in a ring with a central cavity By similarity.

Protein-protein interaction databases

STRINGi70601.PH0452.

Structurei

3D structure databases

ProteinModelPortaliO58221.
SMRiO58221. Positions 897-1096.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini417 – 572156DOD-type homing endonucleaseAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1067.
HOGENOMiHOG000113346.
KOiK04076.
OMAiGLFVKNS.

Family and domain databases

Gene3Di2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.30.230.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR004663. Lon_arc.
IPR027065. Lon_Prtase.
IPR000523. Mg_chelatse_chII.
IPR027417. P-loop_NTPase.
IPR008269. Pept_S16_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR002078. Sigma_54_int.
[Graphical view]
PANTHERiPTHR10046. PTHR10046. 1 hit.
PfamiPF14528. LAGLIDADG_3. 1 hit.
PF05362. Lon_C. 1 hit.
PF01078. Mg_chelatase. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 2 hits.
SSF54211. SSF54211. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR01445. intein_Nterm. 1 hit.
TIGR00764. lon_rel. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O58221-1 [UniParc]FASTAAdd to Basket

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MLSGESEMDE ERMDLGIEFE TTEEIPVPER LIDQVIGQDH AVEVIKTAAK     50
QRRHVLLIGE PGTGKSMLGQ AMAELLPTEE LEDILVFPNP EDENMPRIKT 100
VPAGQGRKIV EEYRRKAKEQ EGVRFYLLFF VFFIVAMAVF LSHGDPNTLL 150
LGVFVILVAL MVTANMRFKT QAMVPKLLVD NSGRKRAPFV DATGAHAGAL 200
LGDVRHDPFQ CFSGEEVIIV EKGKDRKVVK LREFVEDALK EPSGEGMDGD 250
IKVTYKDLRG EDVRILTKDG FVKLLYVNKR EGKQKLRKIV NLDKDYWLAV 300
TPDHKVFTSE GLKEAGEITE KDEIIRVPLV ILDGPKIAST YGEDGKFDDY 350
IRWKKYYEKT GNGYKRAAKE LNIKESTLRW WTQGAKPNSL KMIEELEKLN 400
LLPLTSEDSR LEKVAIILGA LFSDGNIDRN FNTLSFISSE RKAIERFVET 450
LKELFGEFNY EIRDNHESLG KSILFRTWDR RIIRFFVALG APVGNKTKVK 500
LELPWWIKLK PSLFLAFMDG LYSGDGSVPR FARYEEGIKF NGTFEIAQLT 550
DDVEKKLPFF EEIAWYLSFF GIKAKVRVDK TGDKYKVRLI FSQSIDNVLN 600
FLEFIPISLS PAKREKFLRE VESYLAAVPE SSLAGRIEEL REHFNRIKKG 650
ERRSFIETWE VVNVTYNVTT ETGNLLANGL FVKNSGGLGT PAHLRVEPGM 700
IHRAHKGVLF IDEIATLSLK MQQSLLTAMQ EKKFPITGQS ELSSGAMVRT 750
EPVPCDFILV AAGNLDTIEK MHPALRSRIR GYGYEVYMRT TMPDTPENRR 800
KLVQFVAQEV KKDGRIPHFT RDAVEEIVRE AQRRAGRKGH LTLRLRDLGG 850
VVRAAGDIAV RKGKKYVTRE DVLEALKLAK PLEKQLADWY IERKKEYQVI 900
RVEGGEIGRV NGLAIIGEQS GIVLPIEAIV APAASKEEGK IIVTGKLGEI 950
AKEAVLNVSA IIKRYKGEDI SKYDIHVQFL QTYEGVEGDS ASISVATAVI 1000
SALEEIPVRQ DVAMTGSLSV RGEVLPVGGV TPKIEAAIEA GIKTVIIPKS 1050
NEKDVFLSPD KRKKIKIIPV ERIDEVLEVA LVESEKKREL IKRVRESLPL 1100
WMEETPSGET LHEHKGGATL PLEESKA 1127
Length:1,127
Mass (Da):126,992
Last modified:January 1, 1999 - v2
Checksum:iD756DD47332891E1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000001 Genomic DNA. Translation: BAA29538.1.
PIRiE71156.
RefSeqiNP_142432.1. NC_000961.1.
WP_010884561.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA29538; BAA29538; BAA29538.
GeneIDi1444348.
KEGGipho:PH0452.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000001 Genomic DNA. Translation: BAA29538.1 .
PIRi E71156.
RefSeqi NP_142432.1. NC_000961.1.
WP_010884561.1. NC_000961.1.

3D structure databases

ProteinModelPortali O58221.
SMRi O58221. Positions 897-1096.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 70601.PH0452.

Protein family/group databases

MEROPSi S16.005.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA29538 ; BAA29538 ; BAA29538 .
GeneIDi 1444348.
KEGGi pho:PH0452.

Phylogenomic databases

eggNOGi COG1067.
HOGENOMi HOG000113346.
KOi K04076.
OMAi GLFVKNS.

Enzyme and pathway databases

BioCyci PHOR70601:GJWR-450-MONOMER.

Family and domain databases

Gene3Di 2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.30.230.10. 1 hit.
3.40.50.300. 2 hits.
InterProi IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR004663. Lon_arc.
IPR027065. Lon_Prtase.
IPR000523. Mg_chelatse_chII.
IPR027417. P-loop_NTPase.
IPR008269. Pept_S16_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR002078. Sigma_54_int.
[Graphical view ]
PANTHERi PTHR10046. PTHR10046. 1 hit.
Pfami PF14528. LAGLIDADG_3. 1 hit.
PF05362. Lon_C. 1 hit.
PF01078. Mg_chelatase. 1 hit.
[Graphical view ]
PRINTSi PR00379. INTEIN.
SMARTi SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view ]
SUPFAMi SSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 2 hits.
SSF54211. SSF54211. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsi TIGR01445. intein_Nterm. 1 hit.
TIGR00764. lon_rel. 1 hit.
PROSITEi PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Entry informationi

Entry nameiLONB_PYRHO
AccessioniPrimary (citable) accession number: O58221
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: January 1, 1999
Last modified: September 3, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi