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O58221

- LONB_PYRHO

UniProt

O58221 - LONB_PYRHO

Protein

Archaeal Lon protease

Gene

PH0452

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 2 (01 Jan 1999)
      Previous versions | rss
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    Functioni

    ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Degrades polypeptides processively By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei990 – 9901By similarity
    Active sitei1033 – 10331By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi59 – 668ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ATP-dependent peptidase activity Source: InterPro
    3. endonuclease activity Source: UniProtKB-KW
    4. magnesium chelatase activity Source: InterPro
    5. serine-type endopeptidase activity Source: InterPro

    GO - Biological processi

    1. chlorophyll biosynthetic process Source: InterPro
    2. intein-mediated protein splicing Source: InterPro
    3. intron homing Source: UniProtKB-KW
    4. photosynthesis Source: InterPro
    5. protein catabolic process Source: InterPro
    6. regulation of transcription, DNA-templated Source: InterPro

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease, Protease, Serine protease

    Keywords - Biological processi

    Intron homing

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciPHOR70601:GJWR-450-MONOMER.

    Protein family/group databases

    MEROPSiS16.005.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Archaeal Lon protease (EC:3.4.21.-)
    Alternative name(s):
    ATP-dependent protease La homolog
    Cleaved into the following chain:
    Gene namesi
    Ordered Locus Names:PH0452
    OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
    Taxonomic identifieri70601 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    ProteomesiUP000000752: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 210210Archaeal Lon protease, 1st partSequence AnalysisPRO_0000026729Add
    BLAST
    Chaini211 – 684474Pho lon inteinSequence AnalysisPRO_0000026730Add
    BLAST
    Chaini685 – 1127443Archaeal Lon protease, 2nd partSequence AnalysisPRO_0000026731Add
    BLAST

    Post-translational modificationi

    This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

    Keywords - PTMi

    Autocatalytic cleavage, Protein splicing

    Interactioni

    Subunit structurei

    Homohexamer. Organized in a ring with a central cavity By similarity.By similarity

    Protein-protein interaction databases

    STRINGi70601.PH0452.

    Structurei

    3D structure databases

    ProteinModelPortaliO58221.
    SMRiO58221. Positions 897-1096.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 124124CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini167 – 1127961ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei125 – 14521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei146 – 16621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini417 – 572156DOD-type homing endonucleasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 DOD-type homing endonuclease domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1067.
    HOGENOMiHOG000113346.
    KOiK04076.
    OMAiGLFVKNS.

    Family and domain databases

    Gene3Di2.170.16.10. 2 hits.
    3.10.28.10. 1 hit.
    3.30.230.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR004663. Lon_arc.
    IPR027065. Lon_Prtase.
    IPR000523. Mg_chelatse_chII.
    IPR027417. P-loop_NTPase.
    IPR008269. Pept_S16_C.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    IPR002078. Sigma_54_int.
    [Graphical view]
    PANTHERiPTHR10046. PTHR10046. 1 hit.
    PfamiPF14528. LAGLIDADG_3. 1 hit.
    PF05362. Lon_C. 1 hit.
    PF01078. Mg_chelatase. 1 hit.
    [Graphical view]
    PRINTSiPR00379. INTEIN.
    SMARTiSM00305. HintC. 1 hit.
    SM00306. HintN. 1 hit.
    [Graphical view]
    SUPFAMiSSF51294. SSF51294. 2 hits.
    SSF52540. SSF52540. 2 hits.
    SSF54211. SSF54211. 1 hit.
    SSF55608. SSF55608. 1 hit.
    TIGRFAMsiTIGR01445. intein_Nterm. 1 hit.
    TIGR00764. lon_rel. 1 hit.
    PROSITEiPS50818. INTEIN_C_TER. 1 hit.
    PS50819. INTEIN_ENDONUCLEASE. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O58221-1 [UniParc]FASTAAdd to Basket

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    MLSGESEMDE ERMDLGIEFE TTEEIPVPER LIDQVIGQDH AVEVIKTAAK     50
    QRRHVLLIGE PGTGKSMLGQ AMAELLPTEE LEDILVFPNP EDENMPRIKT 100
    VPAGQGRKIV EEYRRKAKEQ EGVRFYLLFF VFFIVAMAVF LSHGDPNTLL 150
    LGVFVILVAL MVTANMRFKT QAMVPKLLVD NSGRKRAPFV DATGAHAGAL 200
    LGDVRHDPFQ CFSGEEVIIV EKGKDRKVVK LREFVEDALK EPSGEGMDGD 250
    IKVTYKDLRG EDVRILTKDG FVKLLYVNKR EGKQKLRKIV NLDKDYWLAV 300
    TPDHKVFTSE GLKEAGEITE KDEIIRVPLV ILDGPKIAST YGEDGKFDDY 350
    IRWKKYYEKT GNGYKRAAKE LNIKESTLRW WTQGAKPNSL KMIEELEKLN 400
    LLPLTSEDSR LEKVAIILGA LFSDGNIDRN FNTLSFISSE RKAIERFVET 450
    LKELFGEFNY EIRDNHESLG KSILFRTWDR RIIRFFVALG APVGNKTKVK 500
    LELPWWIKLK PSLFLAFMDG LYSGDGSVPR FARYEEGIKF NGTFEIAQLT 550
    DDVEKKLPFF EEIAWYLSFF GIKAKVRVDK TGDKYKVRLI FSQSIDNVLN 600
    FLEFIPISLS PAKREKFLRE VESYLAAVPE SSLAGRIEEL REHFNRIKKG 650
    ERRSFIETWE VVNVTYNVTT ETGNLLANGL FVKNSGGLGT PAHLRVEPGM 700
    IHRAHKGVLF IDEIATLSLK MQQSLLTAMQ EKKFPITGQS ELSSGAMVRT 750
    EPVPCDFILV AAGNLDTIEK MHPALRSRIR GYGYEVYMRT TMPDTPENRR 800
    KLVQFVAQEV KKDGRIPHFT RDAVEEIVRE AQRRAGRKGH LTLRLRDLGG 850
    VVRAAGDIAV RKGKKYVTRE DVLEALKLAK PLEKQLADWY IERKKEYQVI 900
    RVEGGEIGRV NGLAIIGEQS GIVLPIEAIV APAASKEEGK IIVTGKLGEI 950
    AKEAVLNVSA IIKRYKGEDI SKYDIHVQFL QTYEGVEGDS ASISVATAVI 1000
    SALEEIPVRQ DVAMTGSLSV RGEVLPVGGV TPKIEAAIEA GIKTVIIPKS 1050
    NEKDVFLSPD KRKKIKIIPV ERIDEVLEVA LVESEKKREL IKRVRESLPL 1100
    WMEETPSGET LHEHKGGATL PLEESKA 1127
    Length:1,127
    Mass (Da):126,992
    Last modified:January 1, 1999 - v2
    Checksum:iD756DD47332891E1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000001 Genomic DNA. Translation: BAA29538.1.
    PIRiE71156.
    RefSeqiNP_142432.1. NC_000961.1.
    WP_010884561.1. NC_000961.1.

    Genome annotation databases

    EnsemblBacteriaiBAA29538; BAA29538; BAA29538.
    GeneIDi1444348.
    KEGGipho:PH0452.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000001 Genomic DNA. Translation: BAA29538.1 .
    PIRi E71156.
    RefSeqi NP_142432.1. NC_000961.1.
    WP_010884561.1. NC_000961.1.

    3D structure databases

    ProteinModelPortali O58221.
    SMRi O58221. Positions 897-1096.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 70601.PH0452.

    Protein family/group databases

    MEROPSi S16.005.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAA29538 ; BAA29538 ; BAA29538 .
    GeneIDi 1444348.
    KEGGi pho:PH0452.

    Phylogenomic databases

    eggNOGi COG1067.
    HOGENOMi HOG000113346.
    KOi K04076.
    OMAi GLFVKNS.

    Enzyme and pathway databases

    BioCyci PHOR70601:GJWR-450-MONOMER.

    Family and domain databases

    Gene3Di 2.170.16.10. 2 hits.
    3.10.28.10. 1 hit.
    3.30.230.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR004663. Lon_arc.
    IPR027065. Lon_Prtase.
    IPR000523. Mg_chelatse_chII.
    IPR027417. P-loop_NTPase.
    IPR008269. Pept_S16_C.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    IPR002078. Sigma_54_int.
    [Graphical view ]
    PANTHERi PTHR10046. PTHR10046. 1 hit.
    Pfami PF14528. LAGLIDADG_3. 1 hit.
    PF05362. Lon_C. 1 hit.
    PF01078. Mg_chelatase. 1 hit.
    [Graphical view ]
    PRINTSi PR00379. INTEIN.
    SMARTi SM00305. HintC. 1 hit.
    SM00306. HintN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51294. SSF51294. 2 hits.
    SSF52540. SSF52540. 2 hits.
    SSF54211. SSF54211. 1 hit.
    SSF55608. SSF55608. 1 hit.
    TIGRFAMsi TIGR01445. intein_Nterm. 1 hit.
    TIGR00764. lon_rel. 1 hit.
    PROSITEi PS50818. INTEIN_C_TER. 1 hit.
    PS50819. INTEIN_ENDONUCLEASE. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

    Entry informationi

    Entry nameiLONB_PYRHO
    AccessioniPrimary (citable) accession number: O58221
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: January 1, 1999
    Last modified: October 1, 2014
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Intein-containing proteins
      List of intein-containing protein entries
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3