Reviewed, UniProtKB/Swiss-Prot O58221 (LONH_PYRHO)

Names and origin

Protein names

Recommended name:
    Putative protease La homolog
    EC=3.4.21.-
Cleaved into the following chain:
    1- Recommended name:
            Pho lon intein

Gene names

Ordered Locus Names:

PH0452

Organism

Pyrococcus horikoshii [Complete proteome] [HAMAP]

Taxonomic identifier

53953 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	1127 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.
Sequence similarities	Belongs to the peptidase S16 family. Contains 1 DOD-type homing endonuclease domain.

Ontologies

Keywords
Biological process	Intron homing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Endonuclease Hydrolase Nuclease Protease Serine protease
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro intron homing Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent peptidase activity Inferred from electronic annotation. Source: InterPro endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro transcription factor binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 210	210	Putative protease La homolog, 1st part Potential		PRO_0000026729
	Chain	211 – 684	474	Pho lon intein Potential		PRO_0000026730
	Chain	685 – 1127	443	Putative protease La homolog, 2nd part Potential		PRO_0000026731
Regions
	Domain	417 – 572	156	DOD-type homing endonuclease
	Nucleotide binding	59 – 66	8	ATP Potential
Sites
	Active site	990	1	By similarity
	Active site	1033	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O58221-1 [UniParc]. Last modified January 1, 1999. Version 2. Checksum: D756DD47332891E1	FASTA	1,127	126,992
	10 20 30 40 50 60 MLSGESEMDE ERMDLGIEFE TTEEIPVPER LIDQVIGQDH AVEVIKTAAK QRRHVLLIGE 70 80 90 100 110 120 PGTGKSMLGQ AMAELLPTEE LEDILVFPNP EDENMPRIKT VPAGQGRKIV EEYRRKAKEQ 130 140 150 160 170 180 EGVRFYLLFF VFFIVAMAVF LSHGDPNTLL LGVFVILVAL MVTANMRFKT QAMVPKLLVD 190 200 210 220 230 240 NSGRKRAPFV DATGAHAGAL LGDVRHDPFQ CFSGEEVIIV EKGKDRKVVK LREFVEDALK 250 260 270 280 290 300 EPSGEGMDGD IKVTYKDLRG EDVRILTKDG FVKLLYVNKR EGKQKLRKIV NLDKDYWLAV 310 320 330 340 350 360 TPDHKVFTSE GLKEAGEITE KDEIIRVPLV ILDGPKIAST YGEDGKFDDY IRWKKYYEKT 370 380 390 400 410 420 GNGYKRAAKE LNIKESTLRW WTQGAKPNSL KMIEELEKLN LLPLTSEDSR LEKVAIILGA 430 440 450 460 470 480 LFSDGNIDRN FNTLSFISSE RKAIERFVET LKELFGEFNY EIRDNHESLG KSILFRTWDR 490 500 510 520 530 540 RIIRFFVALG APVGNKTKVK LELPWWIKLK PSLFLAFMDG LYSGDGSVPR FARYEEGIKF 550 560 570 580 590 600 NGTFEIAQLT DDVEKKLPFF EEIAWYLSFF GIKAKVRVDK TGDKYKVRLI FSQSIDNVLN 610 620 630 640 650 660 FLEFIPISLS PAKREKFLRE VESYLAAVPE SSLAGRIEEL REHFNRIKKG ERRSFIETWE 670 680 690 700 710 720 VVNVTYNVTT ETGNLLANGL FVKNSGGLGT PAHLRVEPGM IHRAHKGVLF IDEIATLSLK 730 740 750 760 770 780 MQQSLLTAMQ EKKFPITGQS ELSSGAMVRT EPVPCDFILV AAGNLDTIEK MHPALRSRIR 790 800 810 820 830 840 GYGYEVYMRT TMPDTPENRR KLVQFVAQEV KKDGRIPHFT RDAVEEIVRE AQRRAGRKGH 850 860 870 880 890 900 LTLRLRDLGG VVRAAGDIAV RKGKKYVTRE DVLEALKLAK PLEKQLADWY IERKKEYQVI 910 920 930 940 950 960 RVEGGEIGRV NGLAIIGEQS GIVLPIEAIV APAASKEEGK IIVTGKLGEI AKEAVLNVSA 970 980 990 1000 1010 1020 IIKRYKGEDI SKYDIHVQFL QTYEGVEGDS ASISVATAVI SALEEIPVRQ DVAMTGSLSV 1030 1040 1050 1060 1070 1080 RGEVLPVGGV TPKIEAAIEA GIKTVIIPKS NEKDVFLSPD KRKKIKIIPV ERIDEVLEVA 1090 1100 1110 1120 LVESEKKREL IKRVRESLPL WMEETPSGET LHEHKGGATL PLEESKA

References

[1]

"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3." Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H. DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OT3.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000001 Genomic DNA. Translation: BAA29538.1.
PIR	E71156.
RefSeq	NP_142432.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	S16.005.
Genome annotation databases
GeneID	1444348.
GenomeReviews	Gene locus PH0452 in contig BA000001_GR.
KEGG	pho:PH0452.
NMPDR	fig|70601.1.peg.448.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG194441.
OMA	ELSSGAM.
Enzyme and pathway databases
BioCyc	PHOR70601:PH0452-MON.
Family and domain databases
InterPro	IPR011704. ATPase_AAA-5. IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splicing_site. IPR004663. Pept_S16_Archaea. IPR008269. Pept_S16_C. IPR001984. Peptidase_S16_C. IPR020568. Ribosomal_S5_D2-typ_fold. IPR002078. RNA_pol_sigma_54_int. [Graphical view]
Pfam	PF07728. AAA_5. 1 hit. PF05362. Lon_C. 1 hit. [Graphical view]
PRINTS	PR00830. ENDOLAPTASE. PR00379. INTEIN.
SMART	SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. PS01046. LON_SER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LONH_PYRHO

Accession

Primary (citable) accession number: O58221

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	January 1, 1999
Last modified:	December 15, 2009
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)