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O58221 (LONB_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Archaeal Lon protease

EC=3.4.21.-
Alternative name(s):
ATP-dependent protease La homolog

Cleaved into the following chain:

  1. Pho lon intein
Gene names
Ordered Locus Names:PH0452
OrganismPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]
Taxonomic identifier70601 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length1127 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Degrades polypeptides processively By similarity.

Subunit structure

Homohexamer. Organized in a ring with a central cavity By similarity.

Subcellular location

Cell membrane By similarity; Multi-pass membrane protein Potential.

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.

Sequence similarities

Belongs to the peptidase S16 family. Archaeal LonB subfamily.

Contains 1 DOD-type homing endonuclease domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 210210Archaeal Lon protease, 1st part Potential
PRO_0000026729
Chain211 – 684474Pho lon intein Potential
PRO_0000026730
Chain685 – 1127443Archaeal Lon protease, 2nd part Potential
PRO_0000026731

Regions

Topological domain1 – 124124Cytoplasmic Potential
Transmembrane125 – 14521Helical; Potential
Transmembrane146 – 16621Helical; Potential
Topological domain167 – 1127961Extracellular Potential
Domain417 – 572156DOD-type homing endonuclease
Nucleotide binding59 – 668ATP Potential

Sites

Active site9901 By similarity
Active site10331 By similarity

Sequences

Sequence LengthMass (Da)Tools
O58221 [UniParc].

Last modified January 1, 1999. Version 2.
Checksum: D756DD47332891E1

FASTA1,127126,992
        10         20         30         40         50         60 
MLSGESEMDE ERMDLGIEFE TTEEIPVPER LIDQVIGQDH AVEVIKTAAK QRRHVLLIGE 

        70         80         90        100        110        120 
PGTGKSMLGQ AMAELLPTEE LEDILVFPNP EDENMPRIKT VPAGQGRKIV EEYRRKAKEQ 

       130        140        150        160        170        180 
EGVRFYLLFF VFFIVAMAVF LSHGDPNTLL LGVFVILVAL MVTANMRFKT QAMVPKLLVD 

       190        200        210        220        230        240 
NSGRKRAPFV DATGAHAGAL LGDVRHDPFQ CFSGEEVIIV EKGKDRKVVK LREFVEDALK 

       250        260        270        280        290        300 
EPSGEGMDGD IKVTYKDLRG EDVRILTKDG FVKLLYVNKR EGKQKLRKIV NLDKDYWLAV 

       310        320        330        340        350        360 
TPDHKVFTSE GLKEAGEITE KDEIIRVPLV ILDGPKIAST YGEDGKFDDY IRWKKYYEKT 

       370        380        390        400        410        420 
GNGYKRAAKE LNIKESTLRW WTQGAKPNSL KMIEELEKLN LLPLTSEDSR LEKVAIILGA 

       430        440        450        460        470        480 
LFSDGNIDRN FNTLSFISSE RKAIERFVET LKELFGEFNY EIRDNHESLG KSILFRTWDR 

       490        500        510        520        530        540 
RIIRFFVALG APVGNKTKVK LELPWWIKLK PSLFLAFMDG LYSGDGSVPR FARYEEGIKF 

       550        560        570        580        590        600 
NGTFEIAQLT DDVEKKLPFF EEIAWYLSFF GIKAKVRVDK TGDKYKVRLI FSQSIDNVLN 

       610        620        630        640        650        660 
FLEFIPISLS PAKREKFLRE VESYLAAVPE SSLAGRIEEL REHFNRIKKG ERRSFIETWE 

       670        680        690        700        710        720 
VVNVTYNVTT ETGNLLANGL FVKNSGGLGT PAHLRVEPGM IHRAHKGVLF IDEIATLSLK 

       730        740        750        760        770        780 
MQQSLLTAMQ EKKFPITGQS ELSSGAMVRT EPVPCDFILV AAGNLDTIEK MHPALRSRIR 

       790        800        810        820        830        840 
GYGYEVYMRT TMPDTPENRR KLVQFVAQEV KKDGRIPHFT RDAVEEIVRE AQRRAGRKGH 

       850        860        870        880        890        900 
LTLRLRDLGG VVRAAGDIAV RKGKKYVTRE DVLEALKLAK PLEKQLADWY IERKKEYQVI 

       910        920        930        940        950        960 
RVEGGEIGRV NGLAIIGEQS GIVLPIEAIV APAASKEEGK IIVTGKLGEI AKEAVLNVSA 

       970        980        990       1000       1010       1020 
IIKRYKGEDI SKYDIHVQFL QTYEGVEGDS ASISVATAVI SALEEIPVRQ DVAMTGSLSV 

      1030       1040       1050       1060       1070       1080 
RGEVLPVGGV TPKIEAAIEA GIKTVIIPKS NEKDVFLSPD KRKKIKIIPV ERIDEVLEVA 

      1090       1100       1110       1120 
LVESEKKREL IKRVRESLPL WMEETPSGET LHEHKGGATL PLEESKA 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000001 Genomic DNA. Translation: BAA29538.1.
PIRE71156.
RefSeqNP_142432.1. NC_000961.1.

3D structure databases

ProteinModelPortalO58221.
SMRO58221. Positions 897-1096.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING70601.PH0452.

Protein family/group databases

MEROPSS16.005.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA29538; BAA29538; BAA29538.
GeneID1444348.
KEGGpho:PH0452.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1067.
HOGENOMHOG000113346.
KOK04076.
OMAGLFVKNS.

Enzyme and pathway databases

BioCycPHOR70601:GJWR-450-MONOMER.

Family and domain databases

Gene3D2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.30.230.10. 1 hit.
3.40.50.300. 2 hits.
InterProIPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR004663. Lon_arc.
IPR027065. Lon_Prtase.
IPR000523. Mg_chelatse_chII.
IPR027417. P-loop_NTPase.
IPR008269. Pept_S16_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR002078. Sigma_54_int.
[Graphical view]
PANTHERPTHR10046. PTHR10046. 1 hit.
PfamPF14528. LAGLIDADG_3. 1 hit.
PF05362. Lon_C. 1 hit.
PF01078. Mg_chelatase. 1 hit.
[Graphical view]
PRINTSPR00379. INTEIN.
SMARTSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMSSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 2 hits.
SSF54211. SSF54211. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsTIGR01445. intein_Nterm. 1 hit.
TIGR00764. lon_rel. 1 hit.
PROSITEPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLONB_PYRHO
AccessionPrimary (citable) accession number: O58221
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: January 1, 1999
Last modified: June 11, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Intein-containing proteins

List of intein-containing protein entries