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O58221

- LONB_PYRHO

UniProt

O58221 - LONB_PYRHO

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Protein

Archaeal Lon protease

Gene

PH0452

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Degrades polypeptides processively (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei990 – 9901By similarity
Active sitei1033 – 10331By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi59 – 668ATPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATP-dependent peptidase activity Source: InterPro
  3. endonuclease activity Source: UniProtKB-KW
  4. magnesium chelatase activity Source: InterPro
  5. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. chlorophyll biosynthetic process Source: InterPro
  2. intein-mediated protein splicing Source: InterPro
  3. intron homing Source: UniProtKB-KW
  4. photosynthesis Source: InterPro
  5. protein catabolic process Source: InterPro
  6. regulation of transcription, DNA-templated Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease, Protease, Serine protease

Keywords - Biological processi

Intron homing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-450-MONOMER.

Protein family/group databases

MEROPSiS16.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Archaeal Lon protease (EC:3.4.21.-)
Alternative name(s):
ATP-dependent protease La homolog
Cleaved into the following chain:
Gene namesi
Ordered Locus Names:PH0452
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000000752: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 210210Archaeal Lon protease, 1st partSequence AnalysisPRO_0000026729Add
BLAST
Chaini211 – 684474Pho lon inteinSequence AnalysisPRO_0000026730Add
BLAST
Chaini685 – 1127443Archaeal Lon protease, 2nd partSequence AnalysisPRO_0000026731Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Subunit structurei

Homohexamer. Organized in a ring with a central cavity (By similarity).By similarity

Protein-protein interaction databases

STRINGi70601.PH0452.

Structurei

3D structure databases

ProteinModelPortaliO58221.
SMRiO58221. Positions 897-1096.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 124124CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini167 – 1127961ExtracellularSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei125 – 14521HelicalSequence AnalysisAdd
BLAST
Transmembranei146 – 16621HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini417 – 572156DOD-type homing endonucleasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 DOD-type homing endonuclease domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1067.
HOGENOMiHOG000113346.
KOiK04076.
OMAiGLFVKNS.

Family and domain databases

Gene3Di2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.30.230.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR004663. Lon_arc.
IPR027065. Lon_Prtase.
IPR000523. Mg_chelatse_chII.
IPR027417. P-loop_NTPase.
IPR008269. Pept_S16_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR002078. Sigma_54_int.
[Graphical view]
PANTHERiPTHR10046. PTHR10046. 1 hit.
PfamiPF14528. LAGLIDADG_3. 1 hit.
PF05362. Lon_C. 1 hit.
PF01078. Mg_chelatase. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 2 hits.
SSF54211. SSF54211. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR01445. intein_Nterm. 1 hit.
TIGR00764. lon_rel. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O58221-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLSGESEMDE ERMDLGIEFE TTEEIPVPER LIDQVIGQDH AVEVIKTAAK
60 70 80 90 100
QRRHVLLIGE PGTGKSMLGQ AMAELLPTEE LEDILVFPNP EDENMPRIKT
110 120 130 140 150
VPAGQGRKIV EEYRRKAKEQ EGVRFYLLFF VFFIVAMAVF LSHGDPNTLL
160 170 180 190 200
LGVFVILVAL MVTANMRFKT QAMVPKLLVD NSGRKRAPFV DATGAHAGAL
210 220 230 240 250
LGDVRHDPFQ CFSGEEVIIV EKGKDRKVVK LREFVEDALK EPSGEGMDGD
260 270 280 290 300
IKVTYKDLRG EDVRILTKDG FVKLLYVNKR EGKQKLRKIV NLDKDYWLAV
310 320 330 340 350
TPDHKVFTSE GLKEAGEITE KDEIIRVPLV ILDGPKIAST YGEDGKFDDY
360 370 380 390 400
IRWKKYYEKT GNGYKRAAKE LNIKESTLRW WTQGAKPNSL KMIEELEKLN
410 420 430 440 450
LLPLTSEDSR LEKVAIILGA LFSDGNIDRN FNTLSFISSE RKAIERFVET
460 470 480 490 500
LKELFGEFNY EIRDNHESLG KSILFRTWDR RIIRFFVALG APVGNKTKVK
510 520 530 540 550
LELPWWIKLK PSLFLAFMDG LYSGDGSVPR FARYEEGIKF NGTFEIAQLT
560 570 580 590 600
DDVEKKLPFF EEIAWYLSFF GIKAKVRVDK TGDKYKVRLI FSQSIDNVLN
610 620 630 640 650
FLEFIPISLS PAKREKFLRE VESYLAAVPE SSLAGRIEEL REHFNRIKKG
660 670 680 690 700
ERRSFIETWE VVNVTYNVTT ETGNLLANGL FVKNSGGLGT PAHLRVEPGM
710 720 730 740 750
IHRAHKGVLF IDEIATLSLK MQQSLLTAMQ EKKFPITGQS ELSSGAMVRT
760 770 780 790 800
EPVPCDFILV AAGNLDTIEK MHPALRSRIR GYGYEVYMRT TMPDTPENRR
810 820 830 840 850
KLVQFVAQEV KKDGRIPHFT RDAVEEIVRE AQRRAGRKGH LTLRLRDLGG
860 870 880 890 900
VVRAAGDIAV RKGKKYVTRE DVLEALKLAK PLEKQLADWY IERKKEYQVI
910 920 930 940 950
RVEGGEIGRV NGLAIIGEQS GIVLPIEAIV APAASKEEGK IIVTGKLGEI
960 970 980 990 1000
AKEAVLNVSA IIKRYKGEDI SKYDIHVQFL QTYEGVEGDS ASISVATAVI
1010 1020 1030 1040 1050
SALEEIPVRQ DVAMTGSLSV RGEVLPVGGV TPKIEAAIEA GIKTVIIPKS
1060 1070 1080 1090 1100
NEKDVFLSPD KRKKIKIIPV ERIDEVLEVA LVESEKKREL IKRVRESLPL
1110 1120
WMEETPSGET LHEHKGGATL PLEESKA
Length:1,127
Mass (Da):126,992
Last modified:January 1, 1999 - v2
Checksum:iD756DD47332891E1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000001 Genomic DNA. Translation: BAA29538.1.
PIRiE71156.
RefSeqiNP_142432.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA29538; BAA29538; BAA29538.
GeneIDi1444348.
KEGGipho:PH0452.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000001 Genomic DNA. Translation: BAA29538.1 .
PIRi E71156.
RefSeqi NP_142432.1. NC_000961.1.

3D structure databases

ProteinModelPortali O58221.
SMRi O58221. Positions 897-1096.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 70601.PH0452.

Protein family/group databases

MEROPSi S16.005.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA29538 ; BAA29538 ; BAA29538 .
GeneIDi 1444348.
KEGGi pho:PH0452.

Phylogenomic databases

eggNOGi COG1067.
HOGENOMi HOG000113346.
KOi K04076.
OMAi GLFVKNS.

Enzyme and pathway databases

BioCyci PHOR70601:GJWR-450-MONOMER.

Family and domain databases

Gene3Di 2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.30.230.10. 1 hit.
3.40.50.300. 2 hits.
InterProi IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR004663. Lon_arc.
IPR027065. Lon_Prtase.
IPR000523. Mg_chelatse_chII.
IPR027417. P-loop_NTPase.
IPR008269. Pept_S16_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR002078. Sigma_54_int.
[Graphical view ]
PANTHERi PTHR10046. PTHR10046. 1 hit.
Pfami PF14528. LAGLIDADG_3. 1 hit.
PF05362. Lon_C. 1 hit.
PF01078. Mg_chelatase. 1 hit.
[Graphical view ]
PRINTSi PR00379. INTEIN.
SMARTi SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view ]
SUPFAMi SSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 2 hits.
SSF54211. SSF54211. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsi TIGR01445. intein_Nterm. 1 hit.
TIGR00764. lon_rel. 1 hit.
PROSITEi PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Entry informationi

Entry nameiLONB_PYRHO
AccessioniPrimary (citable) accession number: O58221
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: January 1, 1999
Last modified: October 29, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3