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Protein

Archaeal Lon protease

Gene

PH0452

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Degrades polypeptides processively (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei990By similarity1
Active sitei1033By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi59 – 66ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease, Protease, Serine protease
Biological processIntron homing
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:G1G39-444-MONOMER

Protein family/group databases

MEROPSiS16.005

Names & Taxonomyi

Protein namesi
Recommended name:
Archaeal Lon protease (EC:3.4.21.-)
Alternative name(s):
ATP-dependent protease La homolog
Cleaved into the following chain:
Gene namesi
Ordered Locus Names:PH0452
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 124CytoplasmicSequence analysisAdd BLAST124
Transmembranei125 – 145HelicalSequence analysisAdd BLAST21
Transmembranei146 – 166HelicalSequence analysisAdd BLAST21
Topological domaini167 – 1127ExtracellularSequence analysisAdd BLAST961

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000267291 – 210Archaeal Lon protease, 1st partSequence analysisAdd BLAST210
ChainiPRO_0000026730211 – 684Pho lon inteinSequence analysisAdd BLAST474
ChainiPRO_0000026731685 – 1127Archaeal Lon protease, 2nd partSequence analysisAdd BLAST443

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Subunit structurei

Homohexamer. Organized in a ring with a central cavity (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi70601.PH0452

Structurei

3D structure databases

ProteinModelPortaliO58221
SMRiO58221
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini417 – 572DOD-type homing endonucleasePROSITE-ProRule annotationAdd BLAST156
Domaini904 – 1083Lon proteolyticPROSITE-ProRule annotationAdd BLAST180

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiarCOG02160 Archaea
arCOG03158 Archaea
ENOG4102TCU Archaea
COG1067 LUCA
COG1372 LUCA
HOGENOMiHOG000113346
KOiK04076
OMAiDPFQCFS
OrthoDBiPOG093Z00OX

Family and domain databases

Gene3Di3.10.28.10, 1 hit
3.30.230.10, 1 hit
InterProiView protein in InterPro
IPR003586 Hint_dom_C
IPR003587 Hint_dom_N
IPR036844 Hint_dom_sf
IPR027434 Homing_endonucl
IPR006142 INTEIN
IPR030934 Intein_C
IPR004042 Intein_endonuc
IPR006141 Intein_N
IPR004663 Lon_arc
IPR008269 Lon_proteolytic
IPR027065 Lon_Prtase
IPR000523 Mg_chelatse_chII_dom
IPR027417 P-loop_NTPase
IPR020568 Ribosomal_S5_D2-typ_fold
IPR014721 Ribosomal_S5_D2-typ_fold_subgr
IPR002078 Sigma_54_int
PANTHERiPTHR10046 PTHR10046, 2 hits
PfamiView protein in Pfam
PF05362 Lon_C, 1 hit
PF01078 Mg_chelatase, 1 hit
PRINTSiPR00379 INTEIN
SMARTiView protein in SMART
SM00305 HintC, 1 hit
SM00306 HintN, 1 hit
SUPFAMiSSF51294 SSF51294, 2 hits
SSF52540 SSF52540, 2 hits
SSF54211 SSF54211, 1 hit
SSF55608 SSF55608, 1 hit
TIGRFAMsiTIGR01445 intein_Nterm, 1 hit
TIGR00764 lon_rel, 1 hit
PROSITEiView protein in PROSITE
PS50818 INTEIN_C_TER, 1 hit
PS50819 INTEIN_ENDONUCLEASE, 1 hit
PS50817 INTEIN_N_TER, 1 hit
PS51786 LON_PROTEOLYTIC, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O58221-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSGESEMDE ERMDLGIEFE TTEEIPVPER LIDQVIGQDH AVEVIKTAAK
60 70 80 90 100
QRRHVLLIGE PGTGKSMLGQ AMAELLPTEE LEDILVFPNP EDENMPRIKT
110 120 130 140 150
VPAGQGRKIV EEYRRKAKEQ EGVRFYLLFF VFFIVAMAVF LSHGDPNTLL
160 170 180 190 200
LGVFVILVAL MVTANMRFKT QAMVPKLLVD NSGRKRAPFV DATGAHAGAL
210 220 230 240 250
LGDVRHDPFQ CFSGEEVIIV EKGKDRKVVK LREFVEDALK EPSGEGMDGD
260 270 280 290 300
IKVTYKDLRG EDVRILTKDG FVKLLYVNKR EGKQKLRKIV NLDKDYWLAV
310 320 330 340 350
TPDHKVFTSE GLKEAGEITE KDEIIRVPLV ILDGPKIAST YGEDGKFDDY
360 370 380 390 400
IRWKKYYEKT GNGYKRAAKE LNIKESTLRW WTQGAKPNSL KMIEELEKLN
410 420 430 440 450
LLPLTSEDSR LEKVAIILGA LFSDGNIDRN FNTLSFISSE RKAIERFVET
460 470 480 490 500
LKELFGEFNY EIRDNHESLG KSILFRTWDR RIIRFFVALG APVGNKTKVK
510 520 530 540 550
LELPWWIKLK PSLFLAFMDG LYSGDGSVPR FARYEEGIKF NGTFEIAQLT
560 570 580 590 600
DDVEKKLPFF EEIAWYLSFF GIKAKVRVDK TGDKYKVRLI FSQSIDNVLN
610 620 630 640 650
FLEFIPISLS PAKREKFLRE VESYLAAVPE SSLAGRIEEL REHFNRIKKG
660 670 680 690 700
ERRSFIETWE VVNVTYNVTT ETGNLLANGL FVKNSGGLGT PAHLRVEPGM
710 720 730 740 750
IHRAHKGVLF IDEIATLSLK MQQSLLTAMQ EKKFPITGQS ELSSGAMVRT
760 770 780 790 800
EPVPCDFILV AAGNLDTIEK MHPALRSRIR GYGYEVYMRT TMPDTPENRR
810 820 830 840 850
KLVQFVAQEV KKDGRIPHFT RDAVEEIVRE AQRRAGRKGH LTLRLRDLGG
860 870 880 890 900
VVRAAGDIAV RKGKKYVTRE DVLEALKLAK PLEKQLADWY IERKKEYQVI
910 920 930 940 950
RVEGGEIGRV NGLAIIGEQS GIVLPIEAIV APAASKEEGK IIVTGKLGEI
960 970 980 990 1000
AKEAVLNVSA IIKRYKGEDI SKYDIHVQFL QTYEGVEGDS ASISVATAVI
1010 1020 1030 1040 1050
SALEEIPVRQ DVAMTGSLSV RGEVLPVGGV TPKIEAAIEA GIKTVIIPKS
1060 1070 1080 1090 1100
NEKDVFLSPD KRKKIKIIPV ERIDEVLEVA LVESEKKREL IKRVRESLPL
1110 1120
WMEETPSGET LHEHKGGATL PLEESKA
Length:1,127
Mass (Da):126,992
Last modified:January 1, 1999 - v2
Checksum:iD756DD47332891E1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA Translation: BAA29538.1
PIRiE71156
RefSeqiWP_010884561.1, NC_000961.1

Genome annotation databases

EnsemblBacteriaiBAA29538; BAA29538; BAA29538
GeneIDi1444348
KEGGipho:PH0452

Similar proteinsi

Entry informationi

Entry nameiLONB_PYRHO
AccessioniPrimary (citable) accession number: O58221
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: January 1, 1999
Last modified: April 25, 2018
This is version 124 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health