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Protein

Adenosylcobinamide-GDP ribazoletransferase

Gene

cobS

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Joins adenosylcobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin). Also synthesizes adenosylcobalamin 5'-phosphate from adenosylcobinamide-GDP and alpha-ribazole 5'-phosphate.UniRule annotation

Catalytic activityi

Adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin.UniRule annotation
Adenosylcobinamide-GDP + alpha-ribazole 5'-phosphate = GMP + adenosylcobalamin 5'-phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: adenosylcobalamin biosynthesis

This protein is involved in step 7 of the subpathway that synthesizes adenosylcobalamin from cob(II)yrinate a,c-diamide.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
  6. no protein annotated in this organism
  7. Adenosylcobinamide-GDP ribazoletransferase (cobS)
This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes adenosylcobalamin from cob(II)yrinate a,c-diamide, the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Cobalamin biosynthesis

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-349-MONOMER.
UniPathwayiUPA00148; UER00238.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosylcobinamide-GDP ribazoletransferaseUniRule annotation (EC:2.7.8.26UniRule annotation)
Alternative name(s):
Cobalamin synthaseUniRule annotation
Cobalamin-5'-phosphate synthaseUniRule annotation
Gene namesi
Name:cobSUniRule annotation
Ordered Locus Names:PH0373
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

  • Cell membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei24 – 4421HelicalUniRule annotationAdd
BLAST
Transmembranei46 – 6621HelicalUniRule annotationAdd
BLAST
Transmembranei96 – 11621HelicalUniRule annotationAdd
BLAST
Transmembranei117 – 13721HelicalUniRule annotationAdd
BLAST
Transmembranei158 – 17821HelicalUniRule annotationAdd
BLAST
Transmembranei184 – 20421HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 230230Adenosylcobinamide-GDP ribazoletransferasePRO_0000146918Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi70601.PH0373.

Family & Domainsi

Sequence similaritiesi

Belongs to the CobS family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiarCOG04338. Archaea.
COG0368. LUCA.
HOGENOMiHOG000228087.
KOiK02233.
OMAiCACCGIP.

Family and domain databases

HAMAPiMF_00719. CobS.
InterProiIPR003805. CobS.
[Graphical view]
PfamiPF02654. CobS. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00317. cobS. 1 hit.

Sequencei

Sequence statusi: Complete.

O58111-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNILPFLTR IPIKGDFEKA RRELWAFPLV SVFTSPLPAL ILYLKVPLAS
60 70 80 90 100
ILALLSLYFT IGLLHLDGLA DWADGIMVKG DREKKIKAMK DINVGVAGIF
110 120 130 140 150
AVVTVLMLQI YSLQLVPFYA IFLAELNSKF AMLLAMATKK PLGKGLGAYF
160 170 180 190 200
MEALDRNQLL YGTLIYLLLY IPVIIAEPRN LVSFLGLFIG LYAVKISLDN
210 220 230
FGGLNGDCIG AVAEITRVGT LVVITLGWRI
Length:230
Mass (Da):25,366
Last modified:August 1, 1998 - v1
Checksum:iB08468A094AFE23A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29447.1.
PIRiB71145.
RefSeqiWP_010884460.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA29447; BAA29447; BAA29447.
GeneIDi1444247.
KEGGipho:PH0373.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29447.1.
PIRiB71145.
RefSeqiWP_010884460.1. NC_000961.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH0373.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA29447; BAA29447; BAA29447.
GeneIDi1444247.
KEGGipho:PH0373.

Phylogenomic databases

eggNOGiarCOG04338. Archaea.
COG0368. LUCA.
HOGENOMiHOG000228087.
KOiK02233.
OMAiCACCGIP.

Enzyme and pathway databases

UniPathwayiUPA00148; UER00238.
BioCyciPHOR70601:GJWR-349-MONOMER.

Family and domain databases

HAMAPiMF_00719. CobS.
InterProiIPR003805. CobS.
[Graphical view]
PfamiPF02654. CobS. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00317. cobS. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Entry informationi

Entry nameiCOBS_PYRHO
AccessioniPrimary (citable) accession number: O58111
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: August 1, 1998
Last modified: November 11, 2015
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.