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Protein

Galactokinase

Gene

galK

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of the gamma-phosphate of ATP to D-galactose to form alpha-D-galactose-1-phosphate (Gal-1-P).UniRule annotation1 Publication

Catalytic activityi

ATP + alpha-D-galactose = ADP + alpha-D-galactose 1-phosphate.UniRule annotation1 Publication

Kineticsi

kcat is 0.91 sec(-1).
  1. KM=403 µM for D-galactose1 Publication
  2. KM=132 µM for ATP1 Publication

    Pathwayi: galactose metabolism

    This protein is involved in the pathway galactose metabolism, which is part of Carbohydrate metabolism.UniRule annotation
    View all proteins of this organism that are known to be involved in the pathway galactose metabolism and in Carbohydrate metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sitei9Transition state stabilizerCurated1
    Binding sitei47ATP1
    Metal bindingi105MagnesiumUniRule annotation1
    Metal bindingi137MagnesiumUniRule annotation1
    Active sitei149Proton acceptorCurated1
    Binding sitei198Substrate1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi99 – 105ATP7

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionKinase, Transferase
    Biological processCarbohydrate metabolism, Galactose metabolism
    LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciPHOR70601:G1G39-344-MONOMER
    UniPathwayiUPA00214

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GalactokinaseUniRule annotation (EC:2.7.1.6UniRule annotation)
    Alternative name(s):
    Galactose kinaseUniRule annotation
    Gene namesi
    Name:galKUniRule annotation
    Ordered Locus Names:PH0369
    OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
    Taxonomic identifieri70601 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    Proteomesi
    • UP000000752 Componenti: Chromosome

    Subcellular locationi

    • Cytoplasm UniRule annotation

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001846421 – 350GalactokinaseAdd BLAST350

    Interactioni

    Protein-protein interaction databases

    STRINGi70601.PH0369

    Structurei

    Secondary structure

    1350
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi2 – 13Combined sources12
    Helixi18 – 20Combined sources3
    Beta strandi23 – 47Combined sources25
    Turni48 – 51Combined sources4
    Beta strandi52 – 56Combined sources5
    Helixi68 – 80Combined sources13
    Beta strandi87 – 93Combined sources7
    Beta strandi100 – 102Combined sources3
    Helixi104 – 119Combined sources16
    Helixi126 – 139Combined sources14
    Helixi148 – 155Combined sources8
    Beta strandi160 – 165Combined sources6
    Turni166 – 168Combined sources3
    Beta strandi171 – 175Combined sources5
    Beta strandi180 – 186Combined sources7
    Helixi191 – 194Combined sources4
    Helixi196 – 212Combined sources17
    Helixi217 – 219Combined sources3
    Helixi222 – 227Combined sources6
    Helixi230 – 254Combined sources25
    Helixi258 – 274Combined sources17
    Helixi281 – 292Combined sources12
    Beta strandi296 – 300Combined sources5
    Beta strandi305 – 314Combined sources10
    Helixi315 – 317Combined sources3
    Helixi318 – 332Combined sources15
    Beta strandi338 – 342Combined sources5

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2CZ9X-ray1.50A1-350[»]
    2DEIX-ray1.70A1-350[»]
    2DEJX-ray1.50A1-350[»]
    ProteinModelPortaliO58107
    SMRiO58107
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO58107

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni15 – 18Substrate binding4

    Sequence similaritiesi

    Belongs to the GHMP kinase family. GalK subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiarCOG01029 Archaea
    COG0153 LUCA
    HOGENOMiHOG000241101
    KOiK00849
    OMAiTHQDYKG
    OrthoDBiPOG093Z05P4

    Family and domain databases

    Gene3Di3.30.230.10, 1 hit
    3.30.70.890, 1 hit
    HAMAPiMF_00246 Galactokinase, 1 hit
    InterProiView protein in InterPro
    IPR000705 Galactokinase
    IPR022963 Galactokinase_bac
    IPR019741 Galactokinase_CS
    IPR019539 GalKase_gal-bd
    IPR013750 GHMP_kinase_C_dom
    IPR036554 GHMP_kinase_C_sf
    IPR006204 GHMP_kinase_N_dom
    IPR006203 GHMP_knse_ATP-bd_CS
    IPR006206 Mevalonate/galactokinase
    IPR020568 Ribosomal_S5_D2-typ_fold
    IPR014721 Ribosomal_S5_D2-typ_fold_subgr
    PANTHERiPTHR10457:SF6 PTHR10457:SF6, 1 hit
    PfamiView protein in Pfam
    PF10509 GalKase_gal_bdg, 1 hit
    PF08544 GHMP_kinases_C, 1 hit
    PF00288 GHMP_kinases_N, 1 hit
    PIRSFiPIRSF000530 Galactokinase, 1 hit
    PRINTSiPR00473 GALCTOKINASE
    SUPFAMiSSF54211 SSF54211, 1 hit
    SSF55060 SSF55060, 1 hit
    TIGRFAMsiTIGR00131 gal_kin, 1 hit
    PROSITEiView protein in PROSITE
    PS00106 GALACTOKINASE, 1 hit
    PS00627 GHMP_KINASES_ATP, 1 hit

    Sequencei

    Sequence statusi: Complete.

    O58107-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MIKVKSPGRV NLIGEHTDYT YGYVMPMAIN LYTKIEAEKH GEVILYSEHF
    60 70 80 90 100
    GEERKFSLND LRKENSWIDY VKGIFWVLKE SDYEVGGIKG RVSGNLPLGA
    110 120 130 140 150
    GLSSSASFEV GILETLDKLY NLKLDSLSKV LLAKKAENEF VGVPCGILDQ
    160 170 180 190 200
    FAVVFGREGN VIFLDTHTLD YEYIPFPKDV SILVFYTGVR RELASSEYAE
    210 220 230 240 250
    RKHIAEESLK ILGKGSSKEV REGELSKLPP LHRKFFGYIV RENARVLEVR
    260 270 280 290 300
    DALKEGNVEE VGKILTTAHW DLAKNYEVSC KELDFFVERA LKLGAYGARL
    310 320 330 340 350
    TGAGFGGSAI ALVDKEDAET IGEEILREYL KRFPWKARHF IVEPSDGVGI
    Length:350
    Mass (Da):39,342
    Last modified:August 1, 1998 - v1
    Checksum:iE2B165005F1CB7D7
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    BA000001 Genomic DNA Translation: BAA29443.1
    PIRiF71144
    RefSeqiWP_010884456.1, NC_000961.1

    Genome annotation databases

    EnsemblBacteriaiBAA29443; BAA29443; BAA29443
    GeneIDi1444243
    KEGGipho:PH0369

    Similar proteinsi

    Entry informationi

    Entry nameiGAL1_PYRHO
    AccessioniPrimary (citable) accession number: O58107
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: August 1, 1998
    Last modified: April 25, 2018
    This is version 124 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome
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    Main funding by: National Institutes of Health