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Protein

Galactokinase

Gene

galK

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of the gamma-phosphate of ATP to D-galactose to form alpha-D-galactose-1-phosphate (Gal-1-P).UniRule annotation1 Publication

Catalytic activityi

ATP + alpha-D-galactose = ADP + alpha-D-galactose 1-phosphate.UniRule annotation1 Publication

Kineticsi

kcat is 0.91 sec(-1).

  1. KM=403 µM for D-galactose1 Publication
  2. KM=132 µM for ATP1 Publication

    Pathwayi: galactose metabolism

    This protein is involved in the pathway galactose metabolism, which is part of Carbohydrate metabolism.UniRule annotation
    View all proteins of this organism that are known to be involved in the pathway galactose metabolism and in Carbohydrate metabolism.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei9 – 91Transition state stabilizerCurated
    Binding sitei47 – 471ATP
    Metal bindingi105 – 1051MagnesiumUniRule annotation
    Metal bindingi137 – 1371MagnesiumUniRule annotation
    Active sitei149 – 1491Proton acceptorCurated
    Binding sitei198 – 1981Substrate

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi99 – 1057ATP

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism, Galactose metabolism

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciPHOR70601:GJWR-345-MONOMER.
    UniPathwayiUPA00214.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GalactokinaseUniRule annotation (EC:2.7.1.6UniRule annotation)
    Alternative name(s):
    Galactose kinaseUniRule annotation
    Gene namesi
    Name:galKUniRule annotation
    Ordered Locus Names:PH0369
    OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
    Taxonomic identifieri70601 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    Proteomesi
    • UP000000752 Componenti: Chromosome

    Subcellular locationi

    • Cytoplasm UniRule annotation

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 350350GalactokinasePRO_0000184642Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi70601.PH0369.

    Structurei

    Secondary structure

    1
    350
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 1312Combined sources
    Helixi18 – 203Combined sources
    Beta strandi23 – 4725Combined sources
    Turni48 – 514Combined sources
    Beta strandi52 – 565Combined sources
    Helixi68 – 8013Combined sources
    Beta strandi87 – 937Combined sources
    Beta strandi100 – 1023Combined sources
    Helixi104 – 11916Combined sources
    Helixi126 – 13914Combined sources
    Helixi148 – 1558Combined sources
    Beta strandi160 – 1656Combined sources
    Turni166 – 1683Combined sources
    Beta strandi171 – 1755Combined sources
    Beta strandi180 – 1867Combined sources
    Helixi191 – 1944Combined sources
    Helixi196 – 21217Combined sources
    Helixi217 – 2193Combined sources
    Helixi222 – 2276Combined sources
    Helixi230 – 25425Combined sources
    Helixi258 – 27417Combined sources
    Helixi281 – 29212Combined sources
    Beta strandi296 – 3005Combined sources
    Beta strandi305 – 31410Combined sources
    Helixi315 – 3173Combined sources
    Helixi318 – 33215Combined sources
    Beta strandi338 – 3425Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2CZ9X-ray1.50A1-350[»]
    2DEIX-ray1.70A1-350[»]
    2DEJX-ray1.50A1-350[»]
    ProteinModelPortaliO58107.
    SMRiO58107. Positions 1-350.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO58107.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni15 – 184Substrate binding

    Sequence similaritiesi

    Belongs to the GHMP kinase family. GalK subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiarCOG01029. Archaea.
    COG0153. LUCA.
    HOGENOMiHOG000241101.
    KOiK00849.
    OMAiQYVGCAC.

    Family and domain databases

    Gene3Di3.30.230.10. 1 hit.
    3.30.70.890. 1 hit.
    HAMAPiMF_00246. Galactokinase.
    InterProiIPR000705. Galactokinase.
    IPR022963. Galactokinase_bac.
    IPR019741. Galactokinase_CS.
    IPR019539. GalKase_gal-bd.
    IPR013750. GHMP_kinase_C_dom.
    IPR006204. GHMP_kinase_N_dom.
    IPR006203. GHMP_knse_ATP-bd_CS.
    IPR006206. Mevalonate/galactokinase.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    [Graphical view]
    PANTHERiPTHR10457. PTHR10457. 1 hit.
    PTHR10457:SF6. PTHR10457:SF6. 1 hit.
    PfamiPF10509. GalKase_gal_bdg. 1 hit.
    PF08544. GHMP_kinases_C. 1 hit.
    PF00288. GHMP_kinases_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000530. Galactokinase. 1 hit.
    PRINTSiPR00473. GALCTOKINASE.
    PR00959. MEVGALKINASE.
    SUPFAMiSSF54211. SSF54211. 1 hit.
    SSF55060. SSF55060. 1 hit.
    TIGRFAMsiTIGR00131. gal_kin. 1 hit.
    PROSITEiPS00106. GALACTOKINASE. 1 hit.
    PS00627. GHMP_KINASES_ATP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O58107-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MIKVKSPGRV NLIGEHTDYT YGYVMPMAIN LYTKIEAEKH GEVILYSEHF
    60 70 80 90 100
    GEERKFSLND LRKENSWIDY VKGIFWVLKE SDYEVGGIKG RVSGNLPLGA
    110 120 130 140 150
    GLSSSASFEV GILETLDKLY NLKLDSLSKV LLAKKAENEF VGVPCGILDQ
    160 170 180 190 200
    FAVVFGREGN VIFLDTHTLD YEYIPFPKDV SILVFYTGVR RELASSEYAE
    210 220 230 240 250
    RKHIAEESLK ILGKGSSKEV REGELSKLPP LHRKFFGYIV RENARVLEVR
    260 270 280 290 300
    DALKEGNVEE VGKILTTAHW DLAKNYEVSC KELDFFVERA LKLGAYGARL
    310 320 330 340 350
    TGAGFGGSAI ALVDKEDAET IGEEILREYL KRFPWKARHF IVEPSDGVGI
    Length:350
    Mass (Da):39,342
    Last modified:August 1, 1998 - v1
    Checksum:iE2B165005F1CB7D7
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    BA000001 Genomic DNA. Translation: BAA29443.1.
    PIRiF71144.
    RefSeqiWP_010884456.1. NC_000961.1.

    Genome annotation databases

    EnsemblBacteriaiBAA29443; BAA29443; BAA29443.
    GeneIDi1444243.
    KEGGipho:PH0369.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    BA000001 Genomic DNA. Translation: BAA29443.1.
    PIRiF71144.
    RefSeqiWP_010884456.1. NC_000961.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2CZ9X-ray1.50A1-350[»]
    2DEIX-ray1.70A1-350[»]
    2DEJX-ray1.50A1-350[»]
    ProteinModelPortaliO58107.
    SMRiO58107. Positions 1-350.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi70601.PH0369.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiBAA29443; BAA29443; BAA29443.
    GeneIDi1444243.
    KEGGipho:PH0369.

    Phylogenomic databases

    eggNOGiarCOG01029. Archaea.
    COG0153. LUCA.
    HOGENOMiHOG000241101.
    KOiK00849.
    OMAiQYVGCAC.

    Enzyme and pathway databases

    UniPathwayiUPA00214.
    BioCyciPHOR70601:GJWR-345-MONOMER.

    Miscellaneous databases

    EvolutionaryTraceiO58107.

    Family and domain databases

    Gene3Di3.30.230.10. 1 hit.
    3.30.70.890. 1 hit.
    HAMAPiMF_00246. Galactokinase.
    InterProiIPR000705. Galactokinase.
    IPR022963. Galactokinase_bac.
    IPR019741. Galactokinase_CS.
    IPR019539. GalKase_gal-bd.
    IPR013750. GHMP_kinase_C_dom.
    IPR006204. GHMP_kinase_N_dom.
    IPR006203. GHMP_knse_ATP-bd_CS.
    IPR006206. Mevalonate/galactokinase.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    [Graphical view]
    PANTHERiPTHR10457. PTHR10457. 1 hit.
    PTHR10457:SF6. PTHR10457:SF6. 1 hit.
    PfamiPF10509. GalKase_gal_bdg. 1 hit.
    PF08544. GHMP_kinases_C. 1 hit.
    PF00288. GHMP_kinases_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000530. Galactokinase. 1 hit.
    PRINTSiPR00473. GALCTOKINASE.
    PR00959. MEVGALKINASE.
    SUPFAMiSSF54211. SSF54211. 1 hit.
    SSF55060. SSF55060. 1 hit.
    TIGRFAMsiTIGR00131. gal_kin. 1 hit.
    PROSITEiPS00106. GALACTOKINASE. 1 hit.
    PS00627. GHMP_KINASES_ATP. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.
    2. "Expression, purification, crystallization and preliminary X-ray diffraction analysis of galactokinase from Pyrococcus horikoshii."
      Inagaki E., Sakamoto K., Obayashi N., Terada T., Shirouzu M., Bessho Y., Kuroishi C., Kuramitsu S., Shinkai A., Yokoyama S.
      Acta Crystallogr. F 62:169-171(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, KINETIC PARAMETERS, CRYSTALLIZATION.
      Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.
    3. "Crystal structure of galactokinase from Pyrococcus horikoshii."
      RIKEN structural genomics initiative (RSGI)
      Submitted (JUL-2011) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.50 ANGSTROMS) OF APOENZYME AND IN COMPLEXES WITH SUBSTRATE AND AN ATP ANALOG.

    Entry informationi

    Entry nameiGAL1_PYRHO
    AccessioniPrimary (citable) accession number: O58107
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: August 1, 1998
    Last modified: February 17, 2016
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.