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Protein

Galactokinase

Gene

galK

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of the gamma-phosphate of ATP to D-galactose to form alpha-D-galactose-1-phosphate (Gal-1-P).UniRule annotation1 Publication

Catalytic activityi

ATP + alpha-D-galactose = ADP + alpha-D-galactose 1-phosphate.UniRule annotation1 Publication

Kineticsi

kcat is 0.91 sec(-1).

  1. KM=403 µM for D-galactose1 Publication
  2. KM=132 µM for ATP1 Publication

    Pathwayi: galactose metabolism

    This protein is involved in the pathway galactose metabolism, which is part of Carbohydrate metabolism.UniRule annotation
    View all proteins of this organism that are known to be involved in the pathway galactose metabolism and in Carbohydrate metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sitei9Transition state stabilizerCurated1
    Binding sitei47ATP1
    Metal bindingi105MagnesiumUniRule annotation1
    Metal bindingi137MagnesiumUniRule annotation1
    Active sitei149Proton acceptorCurated1
    Binding sitei198Substrate1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi99 – 105ATP7

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism, Galactose metabolism

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00214.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GalactokinaseUniRule annotation (EC:2.7.1.6UniRule annotation)
    Alternative name(s):
    Galactose kinaseUniRule annotation
    Gene namesi
    Name:galKUniRule annotation
    Ordered Locus Names:PH0369
    OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
    Taxonomic identifieri70601 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    Proteomesi
    • UP000000752 Componenti: Chromosome

    Subcellular locationi

    • Cytoplasm UniRule annotation

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001846421 – 350GalactokinaseAdd BLAST350

    Interactioni

    Protein-protein interaction databases

    STRINGi70601.PH0369.

    Structurei

    Secondary structure

    1350
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi2 – 13Combined sources12
    Helixi18 – 20Combined sources3
    Beta strandi23 – 47Combined sources25
    Turni48 – 51Combined sources4
    Beta strandi52 – 56Combined sources5
    Helixi68 – 80Combined sources13
    Beta strandi87 – 93Combined sources7
    Beta strandi100 – 102Combined sources3
    Helixi104 – 119Combined sources16
    Helixi126 – 139Combined sources14
    Helixi148 – 155Combined sources8
    Beta strandi160 – 165Combined sources6
    Turni166 – 168Combined sources3
    Beta strandi171 – 175Combined sources5
    Beta strandi180 – 186Combined sources7
    Helixi191 – 194Combined sources4
    Helixi196 – 212Combined sources17
    Helixi217 – 219Combined sources3
    Helixi222 – 227Combined sources6
    Helixi230 – 254Combined sources25
    Helixi258 – 274Combined sources17
    Helixi281 – 292Combined sources12
    Beta strandi296 – 300Combined sources5
    Beta strandi305 – 314Combined sources10
    Helixi315 – 317Combined sources3
    Helixi318 – 332Combined sources15
    Beta strandi338 – 342Combined sources5

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2CZ9X-ray1.50A1-350[»]
    2DEIX-ray1.70A1-350[»]
    2DEJX-ray1.50A1-350[»]
    ProteinModelPortaliO58107.
    SMRiO58107.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO58107.

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni15 – 18Substrate binding4

    Sequence similaritiesi

    Belongs to the GHMP kinase family. GalK subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiarCOG01029. Archaea.
    COG0153. LUCA.
    HOGENOMiHOG000241101.
    KOiK00849.
    OMAiELMTQSH.

    Family and domain databases

    Gene3Di3.30.230.10. 1 hit.
    3.30.70.890. 1 hit.
    HAMAPiMF_00246. Galactokinase. 1 hit.
    InterProiIPR000705. Galactokinase.
    IPR022963. Galactokinase_bac.
    IPR019741. Galactokinase_CS.
    IPR019539. GalKase_gal-bd.
    IPR013750. GHMP_kinase_C_dom.
    IPR006204. GHMP_kinase_N_dom.
    IPR006203. GHMP_knse_ATP-bd_CS.
    IPR006206. Mevalonate/galactokinase.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    [Graphical view]
    PANTHERiPTHR10457. PTHR10457. 1 hit.
    PTHR10457:SF6. PTHR10457:SF6. 1 hit.
    PfamiPF10509. GalKase_gal_bdg. 1 hit.
    PF08544. GHMP_kinases_C. 1 hit.
    PF00288. GHMP_kinases_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000530. Galactokinase. 1 hit.
    PRINTSiPR00473. GALCTOKINASE.
    PR00959. MEVGALKINASE.
    SUPFAMiSSF54211. SSF54211. 1 hit.
    SSF55060. SSF55060. 1 hit.
    TIGRFAMsiTIGR00131. gal_kin. 1 hit.
    PROSITEiPS00106. GALACTOKINASE. 1 hit.
    PS00627. GHMP_KINASES_ATP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O58107-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MIKVKSPGRV NLIGEHTDYT YGYVMPMAIN LYTKIEAEKH GEVILYSEHF
    60 70 80 90 100
    GEERKFSLND LRKENSWIDY VKGIFWVLKE SDYEVGGIKG RVSGNLPLGA
    110 120 130 140 150
    GLSSSASFEV GILETLDKLY NLKLDSLSKV LLAKKAENEF VGVPCGILDQ
    160 170 180 190 200
    FAVVFGREGN VIFLDTHTLD YEYIPFPKDV SILVFYTGVR RELASSEYAE
    210 220 230 240 250
    RKHIAEESLK ILGKGSSKEV REGELSKLPP LHRKFFGYIV RENARVLEVR
    260 270 280 290 300
    DALKEGNVEE VGKILTTAHW DLAKNYEVSC KELDFFVERA LKLGAYGARL
    310 320 330 340 350
    TGAGFGGSAI ALVDKEDAET IGEEILREYL KRFPWKARHF IVEPSDGVGI
    Length:350
    Mass (Da):39,342
    Last modified:August 1, 1998 - v1
    Checksum:iE2B165005F1CB7D7
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    BA000001 Genomic DNA. Translation: BAA29443.1.
    PIRiF71144.
    RefSeqiWP_010884456.1. NC_000961.1.

    Genome annotation databases

    EnsemblBacteriaiBAA29443; BAA29443; BAA29443.
    GeneIDi1444243.
    KEGGipho:PH0369.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    BA000001 Genomic DNA. Translation: BAA29443.1.
    PIRiF71144.
    RefSeqiWP_010884456.1. NC_000961.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2CZ9X-ray1.50A1-350[»]
    2DEIX-ray1.70A1-350[»]
    2DEJX-ray1.50A1-350[»]
    ProteinModelPortaliO58107.
    SMRiO58107.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi70601.PH0369.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiBAA29443; BAA29443; BAA29443.
    GeneIDi1444243.
    KEGGipho:PH0369.

    Phylogenomic databases

    eggNOGiarCOG01029. Archaea.
    COG0153. LUCA.
    HOGENOMiHOG000241101.
    KOiK00849.
    OMAiELMTQSH.

    Enzyme and pathway databases

    UniPathwayiUPA00214.

    Miscellaneous databases

    EvolutionaryTraceiO58107.

    Family and domain databases

    Gene3Di3.30.230.10. 1 hit.
    3.30.70.890. 1 hit.
    HAMAPiMF_00246. Galactokinase. 1 hit.
    InterProiIPR000705. Galactokinase.
    IPR022963. Galactokinase_bac.
    IPR019741. Galactokinase_CS.
    IPR019539. GalKase_gal-bd.
    IPR013750. GHMP_kinase_C_dom.
    IPR006204. GHMP_kinase_N_dom.
    IPR006203. GHMP_knse_ATP-bd_CS.
    IPR006206. Mevalonate/galactokinase.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    [Graphical view]
    PANTHERiPTHR10457. PTHR10457. 1 hit.
    PTHR10457:SF6. PTHR10457:SF6. 1 hit.
    PfamiPF10509. GalKase_gal_bdg. 1 hit.
    PF08544. GHMP_kinases_C. 1 hit.
    PF00288. GHMP_kinases_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000530. Galactokinase. 1 hit.
    PRINTSiPR00473. GALCTOKINASE.
    PR00959. MEVGALKINASE.
    SUPFAMiSSF54211. SSF54211. 1 hit.
    SSF55060. SSF55060. 1 hit.
    TIGRFAMsiTIGR00131. gal_kin. 1 hit.
    PROSITEiPS00106. GALACTOKINASE. 1 hit.
    PS00627. GHMP_KINASES_ATP. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiGAL1_PYRHO
    AccessioniPrimary (citable) accession number: O58107
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: August 1, 1998
    Last modified: November 2, 2016
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.