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Protein

Glutamine synthetase

Gene

glnA

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Probably involved in nitrogen metabolism via ammonium assimilation. Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.By similarity

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.By similarity

Cofactori

Mg2+By similarityNote: Binds 2 Mg2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi126Magnesium 1By similarity1
Metal bindingi128Magnesium 2By similarity1
Binding sitei176ATPBy similarity1
Metal bindingi181Magnesium 2By similarity1
Metal bindingi188Magnesium 2By similarity1
Binding sitei233L-glutamate; via carbonyl oxygenBy similarity1
Metal bindingi237Magnesium 1; via pros nitrogenBy similarity1
Binding sitei241ATPBy similarity1
Binding sitei287L-glutamateBy similarity1
Binding sitei293L-glutamateBy similarity1
Binding sitei305ATPBy similarity1
Binding sitei305L-glutamateBy similarity1
Binding sitei310ATPBy similarity1
Metal bindingi322Magnesium 1By similarity1
Binding sitei324L-glutamateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi239 – 241ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetaseBy similarity (EC:6.3.1.2By similarity)
Short name:
GSBy similarity
Alternative name(s):
Glutamate--ammonia ligaseBy similarity
Glutamine synthetase I alphaBy similarity
Short name:
GSI alphaBy similarity
Gene namesi
Name:glnABy similarity
Ordered Locus Names:PH0359
ORF Names:PHAY031
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001532101 – 443Glutamine synthetaseAdd BLAST443

Proteomic databases

PRIDEiO58097.

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexagons.By similarity

Protein-protein interaction databases

STRINGi70601.PH0359.

Structurei

3D structure databases

ProteinModelPortaliO58097.
SMRiO58097.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.By similarity

Phylogenomic databases

eggNOGiarCOG01909. Archaea.
COG0174. LUCA.
HOGENOMiHOG000005156.
KOiK01915.
OMAiMGFTCYT.
OrthoDBiPOG093Z00GE.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

O58097-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRKVNIIKG NEGQGKRIKF VQLIFVDING MPKGMEVPIT RLEEAIEEGI
60 70 80 90 100
AFDGSSVPGF QGIEDSDLVF KADPSTYVEV PWDNVARVYG YIYKDGKPYE
110 120 130 140 150
ADPRGVLRRT LERLEKLGIK VYIGPEPEFY LFKKNGSWEL EIPDVGGYFD
160 170 180 190 200
ILTLDKAKDI KREIAEYMPY FGLTPEVLHH EVGKAQHEID FRHDEALKTA
210 220 230 240 250
DNIVSFKYIV KAVAEMHGLY ATFMPKPIYG MPGNGMHLHI SLWKDGENIF
260 270 280 290 300
KGEEGLSETA LYFIGGLLKH AKALAAVTNP TVNSYKRLVP GYEAPVYISW
310 320 330 340 350
GYKNRSALIR VPAFWGNGAR IEYRCPDPSA NSYLAFAAIL MAGLDGIKHK
360 370 380 390 400
IEPFAYVEEN VYEMDEKRRE EIGIDMLPEN LGEALDELER DKVVKEALGG
410 420 430 440
AYRNFVGYKR KEWEEYLDYL EAKNLPKDTK NVTEWELERY FFI
Length:443
Mass (Da):50,587
Last modified:August 1, 1998 - v1
Checksum:iB88005ACF23E05C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29433.1.
PIRiD71143.

Genome annotation databases

EnsemblBacteriaiBAA29433; BAA29433; BAA29433.
KEGGipho:PH0359.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29433.1.
PIRiD71143.

3D structure databases

ProteinModelPortaliO58097.
SMRiO58097.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH0359.

Proteomic databases

PRIDEiO58097.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA29433; BAA29433; BAA29433.
KEGGipho:PH0359.

Phylogenomic databases

eggNOGiarCOG01909. Archaea.
COG0174. LUCA.
HOGENOMiHOG000005156.
KOiK01915.
OMAiMGFTCYT.
OrthoDBiPOG093Z00GE.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLN1A_PYRHO
AccessioniPrimary (citable) accession number: O58097
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: April 12, 2017
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.