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O58097 (GLNA_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine synthetase

Short name=GS
EC=6.3.1.2
Alternative name(s):
Glutamate--ammonia ligase
Gene names
Name:glnA
Ordered Locus Names:PH0359
ORF Names:PHAY031
OrganismPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]
Taxonomic identifier70601 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length443 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Enzyme regulation

The activity of this enzyme is controlled by adenylation under conditions of abundant glutamine. The fully adenylated enzyme complex is inactive By similarity.

Subcellular location

Cytoplasm Potential.

Sequence similarities

Belongs to the glutamine synthetase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamine biosynthetic process

Inferred from electronic annotation. Source: InterPro

nitrogen fixation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate-ammonia ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 443443Glutamine synthetase
PRO_0000153210

Amino acid modifications

Modified residue3621O-AMP-tyrosine By similarity

Sequences

Sequence LengthMass (Da)Tools
O58097 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: B88005ACF23E05C0

FASTA44350,587
        10         20         30         40         50         60 
MVRKVNIIKG NEGQGKRIKF VQLIFVDING MPKGMEVPIT RLEEAIEEGI AFDGSSVPGF 

        70         80         90        100        110        120 
QGIEDSDLVF KADPSTYVEV PWDNVARVYG YIYKDGKPYE ADPRGVLRRT LERLEKLGIK 

       130        140        150        160        170        180 
VYIGPEPEFY LFKKNGSWEL EIPDVGGYFD ILTLDKAKDI KREIAEYMPY FGLTPEVLHH 

       190        200        210        220        230        240 
EVGKAQHEID FRHDEALKTA DNIVSFKYIV KAVAEMHGLY ATFMPKPIYG MPGNGMHLHI 

       250        260        270        280        290        300 
SLWKDGENIF KGEEGLSETA LYFIGGLLKH AKALAAVTNP TVNSYKRLVP GYEAPVYISW 

       310        320        330        340        350        360 
GYKNRSALIR VPAFWGNGAR IEYRCPDPSA NSYLAFAAIL MAGLDGIKHK IEPFAYVEEN 

       370        380        390        400        410        420 
VYEMDEKRRE EIGIDMLPEN LGEALDELER DKVVKEALGG AYRNFVGYKR KEWEEYLDYL 

       430        440 
EAKNLPKDTK NVTEWELERY FFI 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000001 Genomic DNA. Translation: BAA29433.1.
PIRD71143.
RefSeqNP_142335.1. NC_000961.1.

3D structure databases

ProteinModelPortalO58097.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING70601.PH0359.

Proteomic databases

PRIDEO58097.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA29433; BAA29433; BAA29433.
GeneID1444235.
KEGGpho:PH0359.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0174.
HOGENOMHOG000005156.
KOK01915.
OMAAEDDVWS.
ProtClustDBCLSK491907.

Enzyme and pathway databases

BioCycPHOR70601:GJWR-337-MONOMER.

Family and domain databases

Gene3D3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMSSF54368. SSF54368. 1 hit.
TIGRFAMsTIGR00653. GlnA. 1 hit.
PROSITEPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLNA_PYRHO
AccessionPrimary (citable) accession number: O58097
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: October 16, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families