Reviewed, UniProtKB/Swiss-Prot O58061 (PUR2_PYRHO)

Names and origin

Protein names

Recommended name:
    Phosphoribosylamine--glycine ligase
    EC=6.3.4.13
Alternative name(s):
    GARS
    Glycinamide ribonucleotide synthetase
    Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	PH0323

Organism

Pyrococcus horikoshii [Complete proteome] [HAMAP]

Taxonomic identifier

53953 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	438 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 438	438	Phosphoribosylamine--glycine ligase HAMAP MF_00138		PRO_0000151516
Regions
	Domain	108 – 316	209	ATP-grasp
	Nucleotide binding	135 – 194	60	ATP By similarity
Sites
	Metal binding	274	1	Magnesium or manganese 1 By similarity
	Metal binding	286	1	Magnesium or manganese 1 By similarity
	Metal binding	286	1	Magnesium or manganese 2 By similarity
	Metal binding	288	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	O58061-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 396EED8AF69A7805	FASTA	438	48,458
	10 20 30 40 50 60 MKVLLVGGGG REHAIGEALV KGGAELYVVS NHRNPGLMRL AKDYGLAKET NVEDVIKFAR 70 80 90 100 110 120 KWGIELAFIG PEAPLEAGIV NALEEEGIPA VGPTREAARL ETNKAWAREF MERNNIPGRK 130 140 150 160 170 180 MFRIFDDVQE MRKWIDEYGK PVVVKPLGLT GGKGVKVVGY QLKDNEEAKE YAEHIIRKDG 190 200 210 220 230 240 KVLIEERTDG VEFTLQVFTD GKKVIPMPLV QDYPHAYEGD VGPITGGMGS YSCSNHLLPF 250 260 270 280 290 300 ITEGDFERAL KTLEETIEAM RKEGYPYKGI LYGQFMLSGE GPVLIEYNAR FGDPEAINVL 310 320 330 340 350 360 AVLDDNLLEI AKGIVEGSLR KAKFLNKATV VKYIAPQGYP QDPIKGIRIE VDEEGIKNEG 370 380 390 400 410 420 AKIIYAAVDE NLTLLGSRAL AIVGVADSLE EAERIAENGV SYVKGPIFYR KDVGTRESVE 430 KRIEIMKKLG KEFEPNLC

References

[1]

"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3." Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H. DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OT3.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000001 Genomic DNA. Translation: BAA29397.1.
PIR	H71138.
RefSeq	NP_142306.1.
3D structure databases
HSSP	HSSP built from PDB template 1GSO based on UniProtKB P15640.
ModBase	Search...
Genome annotation databases
GeneID	1444205.
GenomeReviews	Gene locus PH0323 in contig BA000001_GR.
KEGG	pho:PH0323.
NMPDR	fig|70601.1.peg.305.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O58061.
OMA	O58061. IEYNCRF.
Enzyme and pathway databases
BRENDA	6.3.4.13. 74679.
Family and domain databases
HAMAP	MF_00138. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR000115. Gars. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_PYRHO

Accession

Primary (citable) accession number: O58061

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)