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O58061 (PUR2_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:PH0323
OrganismPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]
Taxonomic identifier70601 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length438 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 438438Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_0000151516

Regions

Domain108 – 316209ATP-grasp
Nucleotide binding135 – 19460ATP By similarity

Sites

Metal binding2741Magnesium or manganese 1 By similarity
Metal binding2861Magnesium or manganese 1 By similarity
Metal binding2861Magnesium or manganese 2 By similarity
Metal binding2881Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
O58061 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 396EED8AF69A7805

FASTA43848,458
        10         20         30         40         50         60 
MKVLLVGGGG REHAIGEALV KGGAELYVVS NHRNPGLMRL AKDYGLAKET NVEDVIKFAR 

        70         80         90        100        110        120 
KWGIELAFIG PEAPLEAGIV NALEEEGIPA VGPTREAARL ETNKAWAREF MERNNIPGRK 

       130        140        150        160        170        180 
MFRIFDDVQE MRKWIDEYGK PVVVKPLGLT GGKGVKVVGY QLKDNEEAKE YAEHIIRKDG 

       190        200        210        220        230        240 
KVLIEERTDG VEFTLQVFTD GKKVIPMPLV QDYPHAYEGD VGPITGGMGS YSCSNHLLPF 

       250        260        270        280        290        300 
ITEGDFERAL KTLEETIEAM RKEGYPYKGI LYGQFMLSGE GPVLIEYNAR FGDPEAINVL 

       310        320        330        340        350        360 
AVLDDNLLEI AKGIVEGSLR KAKFLNKATV VKYIAPQGYP QDPIKGIRIE VDEEGIKNEG 

       370        380        390        400        410        420 
AKIIYAAVDE NLTLLGSRAL AIVGVADSLE EAERIAENGV SYVKGPIFYR KDVGTRESVE 

       430 
KRIEIMKKLG KEFEPNLC 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000001 Genomic DNA. Translation: BAA29397.1.
PIRH71138.
RefSeqNP_142306.1. NC_000961.1.

3D structure databases

ProteinModelPortalO58061.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING70601.PH0323.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA29397; BAA29397; BAA29397.
GeneID1444205.
KEGGpho:PH0323.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033464.
KOK01945.
OMADPEAMNV.
ProtClustDBPRK00885.

Enzyme and pathway databases

BioCycPHOR70601:GJWR-307-MONOMER.
UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_PYRHO
AccessionPrimary (citable) accession number: O58061
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: February 19, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways