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O58061

- PUR2_PYRHO

UniProt

O58061 - PUR2_PYRHO

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Protein

Phosphoribosylamine--glycine ligase

Gene

purD

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation

Cofactori

Binds 2 magnesium or manganese ions per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi274 – 2741Magnesium or manganese 1UniRule annotation
Metal bindingi286 – 2861Magnesium or manganese 1UniRule annotation
Metal bindingi286 – 2861Magnesium or manganese 2UniRule annotation
Metal bindingi288 – 2881Magnesium or manganese 2UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi135 – 19460ATPUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. manganese ion binding Source: UniProtKB-HAMAP
  4. phosphoribosylamine-glycine ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. purine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-307-MONOMER.
UniPathwayiUPA00074; UER00125.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylamine--glycine ligaseUniRule annotation (EC:6.3.4.13UniRule annotation)
Alternative name(s):
GARSUniRule annotation
Glycinamide ribonucleotide synthetaseUniRule annotation
Phosphoribosylglycinamide synthetaseUniRule annotation
Gene namesi
Name:purDUniRule annotation
Ordered Locus Names:PH0323
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000000752: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 438438Phosphoribosylamine--glycine ligasePRO_0000151516Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi70601.PH0323.

Structurei

3D structure databases

ProteinModelPortaliO58061.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini108 – 316209ATP-graspUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the GARS family.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0151.
HOGENOMiHOG000033464.
KOiK01945.
OMAiFIDEYDG.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPiMF_00138. GARS.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR00877. purD. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O58061-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKVLLVGGGG REHAIGEALV KGGAELYVVS NHRNPGLMRL AKDYGLAKET
60 70 80 90 100
NVEDVIKFAR KWGIELAFIG PEAPLEAGIV NALEEEGIPA VGPTREAARL
110 120 130 140 150
ETNKAWAREF MERNNIPGRK MFRIFDDVQE MRKWIDEYGK PVVVKPLGLT
160 170 180 190 200
GGKGVKVVGY QLKDNEEAKE YAEHIIRKDG KVLIEERTDG VEFTLQVFTD
210 220 230 240 250
GKKVIPMPLV QDYPHAYEGD VGPITGGMGS YSCSNHLLPF ITEGDFERAL
260 270 280 290 300
KTLEETIEAM RKEGYPYKGI LYGQFMLSGE GPVLIEYNAR FGDPEAINVL
310 320 330 340 350
AVLDDNLLEI AKGIVEGSLR KAKFLNKATV VKYIAPQGYP QDPIKGIRIE
360 370 380 390 400
VDEEGIKNEG AKIIYAAVDE NLTLLGSRAL AIVGVADSLE EAERIAENGV
410 420 430
SYVKGPIFYR KDVGTRESVE KRIEIMKKLG KEFEPNLC
Length:438
Mass (Da):48,458
Last modified:August 1, 1998 - v1
Checksum:i396EED8AF69A7805
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000001 Genomic DNA. Translation: BAA29397.1.
PIRiH71138.
RefSeqiNP_142306.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA29397; BAA29397; BAA29397.
GeneIDi1444205.
KEGGipho:PH0323.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000001 Genomic DNA. Translation: BAA29397.1 .
PIRi H71138.
RefSeqi NP_142306.1. NC_000961.1.

3D structure databases

ProteinModelPortali O58061.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 70601.PH0323.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA29397 ; BAA29397 ; BAA29397 .
GeneIDi 1444205.
KEGGi pho:PH0323.

Phylogenomic databases

eggNOGi COG0151.
HOGENOMi HOG000033464.
KOi K01945.
OMAi FIDEYDG.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00125 .
BioCyci PHOR70601:GJWR-307-MONOMER.

Family and domain databases

Gene3Di 3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPi MF_00138. GARS.
InterProi IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view ]
Pfami PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view ]
SUPFAMi SSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsi TIGR00877. purD. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Entry informationi

Entry nameiPUR2_PYRHO
AccessioniPrimary (citable) accession number: O58061
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: October 29, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3