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O58054 (PUR5_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylformylglycinamidine cyclo-ligase
EC=6.3.3.1
Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase
Gene names
Name:purM
Ordered Locus Names:PH0316
OrganismPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]
Taxonomic identifier70601 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length334 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP MF_00741_A

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP MF_00741_A

Subcellular location

Cytoplasm By similarity HAMAP MF_00741_A.

Sequence similarities

Belongs to the AIR synthase family.

Ontologies

Keywords
   Biological processPurine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological process'de novo' IMP biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoribosylformylglycinamidine cyclo-ligase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 334334Phosphoribosylformylglycinamidine cyclo-ligase HAMAP MF_00741_A
PRO_0000148287

Sequences

Sequence LengthMass (Da)Tools
O58054 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 89C3B45DFC8CDBA4

FASTA33436,919
        10         20         30         40         50         60 
MLTYAQAGVD EEKTAKALRA IIDAARRTFK FRMNKIGEPG DIGHYSALLD FKDFYLAITT 

        70         80         90        100        110        120 
DGVGTKILVA EAVNKFDTIG IDMIAMNVND LICVGAEPVA LVDYLAVKEP NEDVFQQIAK 

       130        140        150        160        170        180 
GLYEGAKEAG IAIVGGETAV MPDLINGYDL AGTAVGIVEK DKVVTGEKIK PDDIVIGISS 

       190        200        210        220        230        240 
SGIHSNGLTL ARKLLIPKYG LDYEYNGKKL WEWLLEPTRI YVKPILKLIN EVEVHGLAHI 

       250        260        270        280        290        300 
TGGGLLNLKR LTKYGFELEM PPIDGIFKLI YENGVPLEEM FRVFNMGVGF MVIVPQEEKE 

       310        320        330 
NALQILNKYY ESFELGKVIK EPEKIKVKNY GITL

« Hide

References

[1]"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3."
Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. expand/collapse author list , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.
DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000001 Genomic DNA. Translation: BAA29389.1.
PIRF71457.
RefSeqNP_142299.1. NC_000961.1.

3D structure databases

ProteinModelPortalO58054.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBPYRT00000001467; EBPYRP00000001467; EBPYRG00000001467.
GeneID1444194.
GenomeReviewsGene locus PH0316 in contig BA000001_GR.
KEGGpho:PH0316.
NMPDRfig|70601.1.peg.294.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000022555.
HOGENOMHBG531222.
OMAGIDMIAM.
PhylomeDBO58054.
ProtClustDBPRK05385.

Enzyme and pathway databases

BioCycPHOR70601:PH0316-MONOMER.

Family and domain databases

HAMAPMF_00741_A. AIRS_A.
[Tree]
InterProIPR000728. AIR_synth.
IPR010918. AIR_synth_C.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
[Graphical view]
KOK01933.
PfamPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
[Graphical view]
SUPFAMSSF56042. AIR_synth_C. 1 hit.
SSF55326. PurM_N-like. 1 hit.
TIGRFAMsTIGR00878. PurM. 1 hit.
ProtoNetSearch...

Entry information

Entry namePUR5_PYRHO
AccessionPrimary (citable) accession number: O58054
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: August 1, 1998
Last modified: December 14, 2011
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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