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Protein

Valine--tRNA ligase

Gene

valS

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a "posttransfer" editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner.UniRule annotation

Catalytic activityi

ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei539ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Valine--tRNA ligaseUniRule annotation (EC:6.1.1.9UniRule annotation)
Alternative name(s):
Valyl-tRNA synthetaseUniRule annotation
Short name:
ValRSUniRule annotation
Gene namesi
Name:valSUniRule annotation
Ordered Locus Names:PH0314
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001062521 – 891Valine--tRNA ligaseAdd BLAST891

Interactioni

Protein-protein interaction databases

STRINGi70601.PH0314.

Structurei

3D structure databases

ProteinModelPortaliO58052.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi43 – 53"HIGH" regionAdd BLAST11
Motifi536 – 540"KMSKS" region5

Domaini

ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site.UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00808. Archaea.
COG0525. LUCA.
HOGENOMiHOG000020095.
KOiK01873.
OMAiWWRELQL.

Family and domain databases

CDDicd07962. Anticodon_Ia_Val. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02005. Val_tRNA_synth_type2. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033705. Anticodon_Ia_Val.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR022874. Valine-tRNA_ligase_type_2.
IPR002303. Valyl-tRNA_ligase.
[Graphical view]
PANTHERiPTHR11946:SF5. PTHR11946:SF5. 2 hits.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00986. TRNASYNTHVAL.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00422. valS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O58052-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPKNYDPNE IEPKWQKYWL EEKIYKYRLD ENKPSYAIDT PPPFTSGTLH
60 70 80 90 100
LGHVLSHTWI DIIARYKRMR GYNVLFPQGF DNHGLPTELK VEKEFGITKD
110 120 130 140 150
QPEEFLKKCV EWTWQAIEAM RKQFIRIGYS ADWDLEYHTM DDWYKAAVQR
160 170 180 190 200
SLLEFYKKGL IYREEHPVYW CPKCRTSLAK AEVGYVEEEG YLYYIKLPLA
210 220 230 240 250
DGSGYIPIAT TRPELMPACV AVFVHPDDER YKHLVGKKVK LPIFEREVPI
260 270 280 290 300
LADEDVDPNF GTGAVYNCTY GDEQDIVWQK RYNLPVIIVI NEDGTMNENA
310 320 330 340 350
GPYAGLKIEE ARKKIAEDLE KMGLLYKKEK IKHRVLRHTE RSSCMAPIEL
360 370 380 390 400
LPKKQWFIKV KDLIDEIIKV AKEINWYPED MFLRLKDWAE SMDWDWVISR
410 420 430 440 450
QRVFGTPFPF WVCKNGHIIP AREEDLPVDP RFDKPPVDKC PVCGAEIEPV
460 470 480 490 500
TDVLDCWVDS SITPLIITKW HEAIKGDEEA KKWFEHNFPT ALRPQGTDII
510 520 530 540 550
RTWAFYTILR TYVLTGKKPW KDIVINGMVA GPDGRKMSKS YGNVVSPEEV
560 570 580 590 600
IPKYGADALR LWTALAPPGE DHPFKWETVD YNYRFLQKVW NIYRFAERHI
610 620 630 640 650
KDFDYEKYRD VELEPLDKWI LSRLHRIIKF ATEELERYRF NLITRELITF
660 670 680 690 700
IWHEVADDYI EMIKYRLYGE DEESKLKAKV ALYELLYNVM LLLAPFVPHI
710 720 730 740 750
TEEIYHAIFK EKIGEKSVHL LSWPEYREDR IDEEAEKIGE LARKIVSEMR
760 770 780 790 800
KYKNSHGLPL NAKLKHVAIY ALDSYERLKL IERDIAGTMN IERLEIVKGE
810 820 830 840 850
PHLEERIVEV KPNYKNIGPK YGKLVPRIVQ YLRENAESIV REIKEKGKAE
860 870 880 890
FEVEGKKVEL TKEDITVRKE VFSEGEKVET SVVDDVVIVF F
Length:891
Mass (Da):104,961
Last modified:January 24, 2001 - v2
Checksum:i6E3924A940C217FC
GO

Sequence cautioni

The sequence BAA29387 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29387.1. Different initiation.
PIRiD71457.
RefSeqiWP_048053083.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA29387; BAA29387; BAA29387.
GeneIDi1444193.
KEGGipho:PH0314.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29387.1. Different initiation.
PIRiD71457.
RefSeqiWP_048053083.1. NC_000961.1.

3D structure databases

ProteinModelPortaliO58052.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH0314.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA29387; BAA29387; BAA29387.
GeneIDi1444193.
KEGGipho:PH0314.

Phylogenomic databases

eggNOGiarCOG00808. Archaea.
COG0525. LUCA.
HOGENOMiHOG000020095.
KOiK01873.
OMAiWWRELQL.

Family and domain databases

CDDicd07962. Anticodon_Ia_Val. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02005. Val_tRNA_synth_type2. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033705. Anticodon_Ia_Val.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR022874. Valine-tRNA_ligase_type_2.
IPR002303. Valyl-tRNA_ligase.
[Graphical view]
PANTHERiPTHR11946:SF5. PTHR11946:SF5. 2 hits.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00986. TRNASYNTHVAL.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00422. valS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYV_PYRHO
AccessioniPrimary (citable) accession number: O58052
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: January 24, 2001
Last modified: November 2, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.