ID   PCKG_PYRHO              Reviewed;         621 AA.
AC   O58050;
DT   26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   16-JUN-2009, entry version 57.
DE   RecName: Full=Phosphoenolpyruvate carboxykinase [GTP];
DE            Short=PEP carboxykinase;
DE            Short=PEPCK;
DE            EC=4.1.1.32;
DE   AltName: Full=Phosphoenolpyruvate carboxylase;
GN   Name=pckG; OrderedLocusNames=PH0312;
OS   Pyrococcus horikoshii.
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
OX   NCBI_TaxID=53953;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=OT3;
RX   MEDLINE=98344137; PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA   Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y.,
RA   Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y.,
RA   Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y.,
RA   Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A.,
RA   Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K.,
RA   Masuchi Y., Shizuya H., Kikuchi H.;
RT   "Complete sequence and gene organization of the genome of a hyper-
RT   thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL   DNA Res. 5:55-76(1998).
CC   -!- FUNCTION: Catalyzes the conversion of oxaloacetate (OAA) to
CC       phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic
CC       pathway that produces glucose from lactate and other precursors
CC       derived from the citric acid cycle (By similarity).
CC   -!- CATALYTIC ACTIVITY: GTP + oxaloacetate = GDP + phosphoenolpyruvate
CC       + CO(2).
CC   -!- COFACTOR: Binds 1 manganese ion per subunit (By similarity).
CC   -!- PATHWAY: Carbohydrate biosynthesis; gluconeogenesis.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the phosphoenolpyruvate carboxykinase [GTP]
CC       family.
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DR   EMBL; BA000001; BAA29385.1; -; Genomic_DNA.
DR   PIR; B71457; B71457.
DR   RefSeq; NP_142297.1; -.
DR   HSSP; P35558; 1KHB.
DR   GeneID; 1444192; -.
DR   GenomeReviews; BA000001_GR; PH0312.
DR   KEGG; pho:PH0312; -.
DR   NMPDR; fig|70601.1.peg.292; -.
DR   HOGENOM; O58050; -.
DR   OMA; O58050; SHPNARF.
DR   BRENDA; 4.1.1.32; 74679.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005525; F:GTP binding; IEA:HAMAP.
DR   GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004613; F:phosphoenolpyruvate carboxykinase (GTP) act...; IEA:HAMAP.
DR   GO; GO:0006094; P:gluconeogenesis; IEA:HAMAP.
DR   HAMAP; MF_00452; -; 1.
DR   InterPro; IPR018091; PEP_carboxykin_GTP_CS.
DR   InterPro; IPR013035; PEP_carboxykinase_C.
DR   InterPro; IPR008209; PEP_carboxykinase_GTP.
DR   InterPro; IPR008210; PEP_carboxykinase_N.
DR   Gene3D; G3DSA:3.90.228.20; PEP_carboxykinase_C; 1.
DR   Gene3D; G3DSA:3.40.449.10; PEP_carboxykinase_N; 1.
DR   PANTHER; PTHR11561; PEP_carboxykin; 1.
DR   Pfam; PF00821; PEPCK; 1.
DR   PIRSF; PIRSF001348; PEP_carboxykinase_GTP; 1.
DR   ProDom; PD004738; PEPCK_N; 1.
DR   PROSITE; PS00505; PEPCK_GTP; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; Decarboxylase; Gluconeogenesis;
KW   GTP-binding; Lyase; Manganese; Metal-binding; Nucleotide-binding.
FT   CHAIN         1    621       Phosphoenolpyruvate carboxykinase [GTP].
FT                                /FTId=PRO_0000103619.
FT   REGION      381    383       Substrate binding (By similarity).
FT   ACT_SITE    269    269       By similarity.
FT   METAL       226    226       Manganese (By similarity).
FT   METAL       245    245       Manganese (By similarity).
FT   METAL       286    286       Manganese (By similarity).
FT   BINDING      83     83       Substrate (By similarity).
FT   BINDING     219    219       Substrate; via amide nitrogen (By
FT                                similarity).
FT   BINDING     226    226       Substrate (By similarity).
FT   BINDING     267    267       Substrate (By similarity).
FT   BINDING     383    383       GTP (By similarity).
FT   BINDING     415    415       GTP (By similarity).
SQ   SEQUENCE   621 AA;  72202 MW;  738232FF30F20F01 CRC64;
     MEKLRELLPK DQYEKIAAIN NPYLHEFLAE WIEWLEPSKV FVCTDSPEDE EYVRWKALYY
     GEEKMLEIPK HTVHYDNYYD QARDKTNTKL LVPKGVELPF LNTMNREEGL KEIREIMKGI
     MRGKELFICF FVLGPTNSIF TIPAVQLTDS AYVAHSEFLL YRKGYEEFKR LGPTKNFFKF
     VHSAGELDER KTSKNLDKRR IYIDLLDETV YSANTQYGGN TIGLKKLAFR LTIQRAVKEG
     WLSEHMFLMR VNGPNGRKTY FTGAYPSMCG KTSTAMIPWE NIVGDDLVFI KNVDGIARAV
     NVEIGVFGII EGVNQKDDPI IWKVLHSPVE IIFSNVLVKD GKPYWNGMGI EIPDEGENHS
     GKWWRGKRDA EGKEIPPSHK NARFTVRLEA FPNLDREALN NPCGVEVGGM IFGGRDPDTW
     PPVREAFDWE HGVITMGASL ESETTAATLG KEGVRAFNPM SILDFLSVHI GDYIRNYLEF
     GRKLKKTPKI FAVNYFLREN GRWLNEKLDK AVWLKWMELR VHNDVDAIET PIGYIPRYED
     LKVLFKQVLN KDYSREDYEK QFKIRVPELL AKIERIWKIY EPIDNIPEEL FKQLKDERER
     LLKAKAEFGD YISPFTLEKR I
//