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O58050

- PCKG_PYRHO

UniProt

O58050 - PCKG_PYRHO

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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Binds 1 manganese ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei83 – 831SubstrateUniRule annotation
Binding sitei219 – 2191Substrate; via amide nitrogenUniRule annotation
Metal bindingi226 – 2261ManganeseUniRule annotation
Binding sitei226 – 2261SubstrateUniRule annotation
Metal bindingi245 – 2451Manganese; via tele nitrogenUniRule annotation
Binding sitei267 – 2671SubstrateUniRule annotation
Active sitei269 – 2691UniRule annotation
Metal bindingi286 – 2861ManganeseUniRule annotation
Binding sitei383 – 3831GTPUniRule annotation
Binding sitei415 – 4151GTPUniRule annotation

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-294-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:PH0312
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000000752: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 621621Phosphoenolpyruvate carboxykinase [GTP]PRO_0000103619Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi70601.PH0312.

Structurei

3D structure databases

ProteinModelPortaliO58050.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni381 – 3833Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiSEFLLYR.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O58050-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEKLRELLPK DQYEKIAAIN NPYLHEFLAE WIEWLEPSKV FVCTDSPEDE
60 70 80 90 100
EYVRWKALYY GEEKMLEIPK HTVHYDNYYD QARDKTNTKL LVPKGVELPF
110 120 130 140 150
LNTMNREEGL KEIREIMKGI MRGKELFICF FVLGPTNSIF TIPAVQLTDS
160 170 180 190 200
AYVAHSEFLL YRKGYEEFKR LGPTKNFFKF VHSAGELDER KTSKNLDKRR
210 220 230 240 250
IYIDLLDETV YSANTQYGGN TIGLKKLAFR LTIQRAVKEG WLSEHMFLMR
260 270 280 290 300
VNGPNGRKTY FTGAYPSMCG KTSTAMIPWE NIVGDDLVFI KNVDGIARAV
310 320 330 340 350
NVEIGVFGII EGVNQKDDPI IWKVLHSPVE IIFSNVLVKD GKPYWNGMGI
360 370 380 390 400
EIPDEGENHS GKWWRGKRDA EGKEIPPSHK NARFTVRLEA FPNLDREALN
410 420 430 440 450
NPCGVEVGGM IFGGRDPDTW PPVREAFDWE HGVITMGASL ESETTAATLG
460 470 480 490 500
KEGVRAFNPM SILDFLSVHI GDYIRNYLEF GRKLKKTPKI FAVNYFLREN
510 520 530 540 550
GRWLNEKLDK AVWLKWMELR VHNDVDAIET PIGYIPRYED LKVLFKQVLN
560 570 580 590 600
KDYSREDYEK QFKIRVPELL AKIERIWKIY EPIDNIPEEL FKQLKDERER
610 620
LLKAKAEFGD YISPFTLEKR I
Length:621
Mass (Da):72,202
Last modified:August 1, 1998 - v1
Checksum:i738232FF30F20F01
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000001 Genomic DNA. Translation: BAA29385.1.
PIRiB71457.
RefSeqiNP_142297.1. NC_000961.1.
WP_010884405.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA29385; BAA29385; BAA29385.
GeneIDi1444192.
KEGGipho:PH0312.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000001 Genomic DNA. Translation: BAA29385.1 .
PIRi B71457.
RefSeqi NP_142297.1. NC_000961.1.
WP_010884405.1. NC_000961.1.

3D structure databases

ProteinModelPortali O58050.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 70601.PH0312.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA29385 ; BAA29385 ; BAA29385 .
GeneIDi 1444192.
KEGGi pho:PH0312.

Phylogenomic databases

eggNOGi COG1274.
HOGENOMi HOG000191700.
KOi K01596.
OMAi SEFLLYR.

Enzyme and pathway databases

UniPathwayi UPA00138 .
BioCyci PHOR70601:GJWR-294-MONOMER.

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Entry informationi

Entry nameiPCKG_PYRHO
AccessioniPrimary (citable) accession number: O58050
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: August 1, 1998
Last modified: October 29, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3