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O58050

- PCKG_PYRHO

UniProt

O58050 - PCKG_PYRHO

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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene
pckG, PH0312
Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Binds 1 manganese ion per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei83 – 831Substrate By similarity
Binding sitei219 – 2191Substrate; via amide nitrogen By similarity
Metal bindingi226 – 2261Manganese By similarity
Binding sitei226 – 2261Substrate By similarity
Metal bindingi245 – 2451Manganese; via tele nitrogen By similarity
Binding sitei267 – 2671Substrate By similarity
Active sitei269 – 2691 By similarity
Metal bindingi286 – 2861Manganese By similarity
Binding sitei383 – 3831GTP By similarity
Binding sitei415 – 4151GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-294-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP] (EC:4.1.1.32)
Short name:
PEP carboxykinase
Short name:
PEPCK
Gene namesi
Name:pckG
Ordered Locus Names:PH0312
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000000752: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 621621Phosphoenolpyruvate carboxykinase [GTP]UniRule annotationPRO_0000103619Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi70601.PH0312.

Structurei

3D structure databases

ProteinModelPortaliO58050.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni381 – 3833Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiSEFLLYR.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O58050-1 [UniParc]FASTAAdd to Basket

« Hide

MEKLRELLPK DQYEKIAAIN NPYLHEFLAE WIEWLEPSKV FVCTDSPEDE    50
EYVRWKALYY GEEKMLEIPK HTVHYDNYYD QARDKTNTKL LVPKGVELPF 100
LNTMNREEGL KEIREIMKGI MRGKELFICF FVLGPTNSIF TIPAVQLTDS 150
AYVAHSEFLL YRKGYEEFKR LGPTKNFFKF VHSAGELDER KTSKNLDKRR 200
IYIDLLDETV YSANTQYGGN TIGLKKLAFR LTIQRAVKEG WLSEHMFLMR 250
VNGPNGRKTY FTGAYPSMCG KTSTAMIPWE NIVGDDLVFI KNVDGIARAV 300
NVEIGVFGII EGVNQKDDPI IWKVLHSPVE IIFSNVLVKD GKPYWNGMGI 350
EIPDEGENHS GKWWRGKRDA EGKEIPPSHK NARFTVRLEA FPNLDREALN 400
NPCGVEVGGM IFGGRDPDTW PPVREAFDWE HGVITMGASL ESETTAATLG 450
KEGVRAFNPM SILDFLSVHI GDYIRNYLEF GRKLKKTPKI FAVNYFLREN 500
GRWLNEKLDK AVWLKWMELR VHNDVDAIET PIGYIPRYED LKVLFKQVLN 550
KDYSREDYEK QFKIRVPELL AKIERIWKIY EPIDNIPEEL FKQLKDERER 600
LLKAKAEFGD YISPFTLEKR I 621
Length:621
Mass (Da):72,202
Last modified:August 1, 1998 - v1
Checksum:i738232FF30F20F01
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000001 Genomic DNA. Translation: BAA29385.1.
PIRiB71457.
RefSeqiNP_142297.1. NC_000961.1.
WP_010884405.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA29385; BAA29385; BAA29385.
GeneIDi1444192.
KEGGipho:PH0312.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000001 Genomic DNA. Translation: BAA29385.1 .
PIRi B71457.
RefSeqi NP_142297.1. NC_000961.1.
WP_010884405.1. NC_000961.1.

3D structure databases

ProteinModelPortali O58050.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 70601.PH0312.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA29385 ; BAA29385 ; BAA29385 .
GeneIDi 1444192.
KEGGi pho:PH0312.

Phylogenomic databases

eggNOGi COG1274.
HOGENOMi HOG000191700.
KOi K01596.
OMAi SEFLLYR.

Enzyme and pathway databases

UniPathwayi UPA00138 .
BioCyci PHOR70601:GJWR-294-MONOMER.

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Entry informationi

Entry nameiPCKG_PYRHO
AccessioniPrimary (citable) accession number: O58050
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: August 1, 1998
Last modified: September 3, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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