Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O58050

- PCKG_PYRHO

UniProt

O58050 - PCKG_PYRHO

Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

    Catalytic activityi

    GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

    Cofactori

    Binds 1 manganese ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei83 – 831SubstrateUniRule annotation
    Binding sitei219 – 2191Substrate; via amide nitrogenUniRule annotation
    Metal bindingi226 – 2261ManganeseUniRule annotation
    Binding sitei226 – 2261SubstrateUniRule annotation
    Metal bindingi245 – 2451Manganese; via tele nitrogenUniRule annotation
    Binding sitei267 – 2671SubstrateUniRule annotation
    Active sitei269 – 2691UniRule annotation
    Metal bindingi286 – 2861ManganeseUniRule annotation
    Binding sitei383 – 3831GTPUniRule annotation
    Binding sitei415 – 4151GTPUniRule annotation

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-HAMAP
    2. manganese ion binding Source: UniProtKB-HAMAP
    3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. gluconeogenesis Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Gluconeogenesis

    Keywords - Ligandi

    GTP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciPHOR70601:GJWR-294-MONOMER.
    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
    Short name:
    PEP carboxykinaseUniRule annotation
    Short name:
    PEPCKUniRule annotation
    Gene namesi
    Name:pckGUniRule annotation
    Ordered Locus Names:PH0312
    OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
    Taxonomic identifieri70601 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    ProteomesiUP000000752: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 621621Phosphoenolpyruvate carboxykinase [GTP]PRO_0000103619Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi70601.PH0312.

    Structurei

    3D structure databases

    ProteinModelPortaliO58050.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni381 – 3833Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1274.
    HOGENOMiHOG000191700.
    KOiK01596.
    OMAiSEFLLYR.

    Family and domain databases

    Gene3Di3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPiMF_00452. PEPCK_GTP.
    InterProiIPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view]
    PANTHERiPTHR11561. PTHR11561. 1 hit.
    PfamiPF00821. PEPCK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMiSSF68923. SSF68923. 1 hit.
    PROSITEiPS00505. PEPCK_GTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O58050-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEKLRELLPK DQYEKIAAIN NPYLHEFLAE WIEWLEPSKV FVCTDSPEDE    50
    EYVRWKALYY GEEKMLEIPK HTVHYDNYYD QARDKTNTKL LVPKGVELPF 100
    LNTMNREEGL KEIREIMKGI MRGKELFICF FVLGPTNSIF TIPAVQLTDS 150
    AYVAHSEFLL YRKGYEEFKR LGPTKNFFKF VHSAGELDER KTSKNLDKRR 200
    IYIDLLDETV YSANTQYGGN TIGLKKLAFR LTIQRAVKEG WLSEHMFLMR 250
    VNGPNGRKTY FTGAYPSMCG KTSTAMIPWE NIVGDDLVFI KNVDGIARAV 300
    NVEIGVFGII EGVNQKDDPI IWKVLHSPVE IIFSNVLVKD GKPYWNGMGI 350
    EIPDEGENHS GKWWRGKRDA EGKEIPPSHK NARFTVRLEA FPNLDREALN 400
    NPCGVEVGGM IFGGRDPDTW PPVREAFDWE HGVITMGASL ESETTAATLG 450
    KEGVRAFNPM SILDFLSVHI GDYIRNYLEF GRKLKKTPKI FAVNYFLREN 500
    GRWLNEKLDK AVWLKWMELR VHNDVDAIET PIGYIPRYED LKVLFKQVLN 550
    KDYSREDYEK QFKIRVPELL AKIERIWKIY EPIDNIPEEL FKQLKDERER 600
    LLKAKAEFGD YISPFTLEKR I 621
    Length:621
    Mass (Da):72,202
    Last modified:August 1, 1998 - v1
    Checksum:i738232FF30F20F01
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000001 Genomic DNA. Translation: BAA29385.1.
    PIRiB71457.
    RefSeqiNP_142297.1. NC_000961.1.
    WP_010884405.1. NC_000961.1.

    Genome annotation databases

    EnsemblBacteriaiBAA29385; BAA29385; BAA29385.
    GeneIDi1444192.
    KEGGipho:PH0312.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000001 Genomic DNA. Translation: BAA29385.1 .
    PIRi B71457.
    RefSeqi NP_142297.1. NC_000961.1.
    WP_010884405.1. NC_000961.1.

    3D structure databases

    ProteinModelPortali O58050.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 70601.PH0312.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAA29385 ; BAA29385 ; BAA29385 .
    GeneIDi 1444192.
    KEGGi pho:PH0312.

    Phylogenomic databases

    eggNOGi COG1274.
    HOGENOMi HOG000191700.
    KOi K01596.
    OMAi SEFLLYR.

    Enzyme and pathway databases

    UniPathwayi UPA00138 .
    BioCyci PHOR70601:GJWR-294-MONOMER.

    Family and domain databases

    Gene3Di 3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPi MF_00452. PEPCK_GTP.
    InterProi IPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view ]
    PANTHERi PTHR11561. PTHR11561. 1 hit.
    Pfami PF00821. PEPCK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMi SSF68923. SSF68923. 1 hit.
    PROSITEi PS00505. PEPCK_GTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

    Entry informationi

    Entry nameiPCKG_PYRHO
    AccessioniPrimary (citable) accession number: O58050
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3