Reviewed, UniProtKB/Swiss-Prot O58050 (PCKG_PYRHO)

Names and origin

Protein names

Recommended name:
    Phosphoenolpyruvate carboxykinase [GTP]
      Short name=PEP carboxykinase
      Short name=PEPCK
    EC=4.1.1.32
Alternative name(s):
    Phosphoenolpyruvate carboxylase

Gene names

Name:	pckG
Ordered Locus Names:	PH0312

Organism

Pyrococcus horikoshii [Complete proteome] [HAMAP]

Taxonomic identifier

53953 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	621 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity.
Catalytic activity	GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2. HAMAP MF_00452
Cofactor	Binds 1 manganese ion per subunit By similarity.
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00452
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Ontologies

Keywords
Biological process	Gluconeogenesis
Cellular component	Cytoplasm
Ligand	GTP-binding Manganese Metal-binding Nucleotide-binding
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoenolpyruvate carboxykinase (GTP) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 621	621	Phosphoenolpyruvate carboxykinase [GTP] HAMAP MF_00452		PRO_0000103619
Regions
	Region	381 – 383	3	Substrate binding By similarity
Sites
	Active site	269	1	By similarity
	Metal binding	226	1	Manganese By similarity
	Metal binding	245	1	Manganese By similarity
	Metal binding	286	1	Manganese By similarity
	Binding site	83	1	Substrate By similarity
	Binding site	219	1	Substrate; via amide nitrogen By similarity
	Binding site	226	1	Substrate By similarity
	Binding site	267	1	Substrate By similarity
	Binding site	383	1	GTP By similarity
	Binding site	415	1	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O58050-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 738232FF30F20F01	FASTA	621	72,202
	10 20 30 40 50 60 MEKLRELLPK DQYEKIAAIN NPYLHEFLAE WIEWLEPSKV FVCTDSPEDE EYVRWKALYY 70 80 90 100 110 120 GEEKMLEIPK HTVHYDNYYD QARDKTNTKL LVPKGVELPF LNTMNREEGL KEIREIMKGI 130 140 150 160 170 180 MRGKELFICF FVLGPTNSIF TIPAVQLTDS AYVAHSEFLL YRKGYEEFKR LGPTKNFFKF 190 200 210 220 230 240 VHSAGELDER KTSKNLDKRR IYIDLLDETV YSANTQYGGN TIGLKKLAFR LTIQRAVKEG 250 260 270 280 290 300 WLSEHMFLMR VNGPNGRKTY FTGAYPSMCG KTSTAMIPWE NIVGDDLVFI KNVDGIARAV 310 320 330 340 350 360 NVEIGVFGII EGVNQKDDPI IWKVLHSPVE IIFSNVLVKD GKPYWNGMGI EIPDEGENHS 370 380 390 400 410 420 GKWWRGKRDA EGKEIPPSHK NARFTVRLEA FPNLDREALN NPCGVEVGGM IFGGRDPDTW 430 440 450 460 470 480 PPVREAFDWE HGVITMGASL ESETTAATLG KEGVRAFNPM SILDFLSVHI GDYIRNYLEF 490 500 510 520 530 540 GRKLKKTPKI FAVNYFLREN GRWLNEKLDK AVWLKWMELR VHNDVDAIET PIGYIPRYED 550 560 570 580 590 600 LKVLFKQVLN KDYSREDYEK QFKIRVPELL AKIERIWKIY EPIDNIPEEL FKQLKDERER 610 620 LLKAKAEFGD YISPFTLEKR I

References

[1]

"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3." Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H. DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OT3.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000001 Genomic DNA. Translation: BAA29385.1.
PIR	B71457.
RefSeq	NP_142297.1.
3D structure databases
HSSP	HSSP built from PDB template 1KHB based on UniProtKB P35558.
ModBase	Search...
Genome annotation databases
GeneID	1444192.
GenomeReviews	Gene locus PH0312 in contig BA000001_GR.
KEGG	pho:PH0312.
NMPDR	fig|70601.1.peg.292.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O58050.
OMA	O58050. SHPNARF.
Enzyme and pathway databases
BRENDA	4.1.1.32. 74679.
Family and domain databases
HAMAP	MF_00452. [Tree]
InterPro	IPR018091. PEP_carboxykin_GTP_CS. IPR013035. PEP_carboxykinase_C. IPR008209. PEP_carboxykinase_GTP. IPR008210. PEP_carboxykinase_N. [Graphical view]
Gene3D	G3DSA:3.90.228.20. PEP_carboxykinase_C. 1 hit. G3DSA:3.40.449.10. PEP_carboxykinase_N. 1 hit.
PANTHER	PTHR11561. PEP_carboxykin. 1 hit.
Pfam	PF00821. PEPCK. 1 hit. [Graphical view]
PIRSF	PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
ProDom	PD004738. PEPCK_N. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00505. PEPCK_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PCKG_PYRHO

Accession

Primary (citable) accession number: O58050

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	August 1, 1998
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)