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O58050 (PCKG_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxykinase [GTP]
Short name=PEP carboxykinase
Short name=PEPCK
EC=4.1.1.32
Alternative name(s):
Phosphoenolpyruvate carboxylase
Gene names
Name:pckG
Ordered Locus Names:PH0312
OrganismPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]
Taxonomic identifier70601 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length621 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity. HAMAP MF_00452

Catalytic activity

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2. HAMAP MF_00452

Cofactor

Binds 1 manganese ion per subunit By similarity. HAMAP MF_00452

Pathway

Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00452

Subcellular location

Cytoplasm By similarity HAMAP MF_00452.

Sequence similarities

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Ontologies

Keywords
   Biological processGluconeogenesis
   Cellular componentCytoplasm
   LigandGTP-binding
Manganese
Metal-binding
Nucleotide-binding
   Molecular functionDecarboxylase
Lyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processgluconeogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoenolpyruvate carboxykinase (GTP) activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 621621Phosphoenolpyruvate carboxykinase [GTP] HAMAP MF_00452
PRO_0000103619

Regions

Region381 – 3833Substrate binding By similarity

Sites

Active site2691 By similarity
Metal binding2261Manganese By similarity
Metal binding2451Manganese By similarity
Metal binding2861Manganese By similarity
Binding site831Substrate By similarity
Binding site2191Substrate; via amide nitrogen By similarity
Binding site2261Substrate By similarity
Binding site2671Substrate By similarity
Binding site3831GTP By similarity
Binding site4151GTP By similarity

Sequences

Sequence LengthMass (Da)Tools
O58050 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 738232FF30F20F01

FASTA62172,202
        10         20         30         40         50         60 
MEKLRELLPK DQYEKIAAIN NPYLHEFLAE WIEWLEPSKV FVCTDSPEDE EYVRWKALYY 

        70         80         90        100        110        120 
GEEKMLEIPK HTVHYDNYYD QARDKTNTKL LVPKGVELPF LNTMNREEGL KEIREIMKGI 

       130        140        150        160        170        180 
MRGKELFICF FVLGPTNSIF TIPAVQLTDS AYVAHSEFLL YRKGYEEFKR LGPTKNFFKF 

       190        200        210        220        230        240 
VHSAGELDER KTSKNLDKRR IYIDLLDETV YSANTQYGGN TIGLKKLAFR LTIQRAVKEG 

       250        260        270        280        290        300 
WLSEHMFLMR VNGPNGRKTY FTGAYPSMCG KTSTAMIPWE NIVGDDLVFI KNVDGIARAV 

       310        320        330        340        350        360 
NVEIGVFGII EGVNQKDDPI IWKVLHSPVE IIFSNVLVKD GKPYWNGMGI EIPDEGENHS 

       370        380        390        400        410        420 
GKWWRGKRDA EGKEIPPSHK NARFTVRLEA FPNLDREALN NPCGVEVGGM IFGGRDPDTW 

       430        440        450        460        470        480 
PPVREAFDWE HGVITMGASL ESETTAATLG KEGVRAFNPM SILDFLSVHI GDYIRNYLEF 

       490        500        510        520        530        540 
GRKLKKTPKI FAVNYFLREN GRWLNEKLDK AVWLKWMELR VHNDVDAIET PIGYIPRYED 

       550        560        570        580        590        600 
LKVLFKQVLN KDYSREDYEK QFKIRVPELL AKIERIWKIY EPIDNIPEEL FKQLKDERER 

       610        620 
LLKAKAEFGD YISPFTLEKR I

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References

[1]"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3."
Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. expand/collapse author list , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.
DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000001 Genomic DNA. Translation: BAA29385.1.
PIRB71457.
RefSeqNP_142297.1. NC_000961.1.

3D structure databases

ProteinModelPortalO58050.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBPYRT00000001846; EBPYRP00000001846; EBPYRG00000001846.
GeneID1444192.
GenomeReviewsGene locus PH0312 in contig BA000001_GR.
KEGGpho:PH0312.
NMPDRfig|70601.1.peg.292.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000022626.
HOGENOMHBG484437.
OMACGYHMGD.
ProtClustDBPRK04210.

Enzyme and pathway databases

BioCycPHOR70601:PH0312-MONOMER.

Family and domain databases

HAMAPMF_00452. PEPCK_GTP.
[Tree]
InterProIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
Gene3DG3DSA:3.90.228.20. PEP_carboxykinase_C. 2 hits.
G3DSA:3.40.449.10. PEP_carboxykinase_N. 1 hit.
KOK01596.
PANTHERPTHR11561. PEP_carboxykin. 1 hit.
PfamPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMSSF68923. PEP_carboxykinase_N. 1 hit.
PROSITEPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePCKG_PYRHO
AccessionPrimary (citable) accession number: O58050
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: August 1, 1998
Last modified: November 16, 2011
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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