O58033 (ENDA_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA-splicing endonuclease
EC=3.1.27.9

Alternative name(s):
tRNA-intron endonuclease

Gene names

Name:	endA
Ordered Locus Names:	PH0295

Organism

Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]

Taxonomic identifier

70601 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus › Pyrococcus horikoshii

Protein attributes

Sequence length	170 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp By similarity. HAMAP-Rule MF_01833
Catalytic activity	Endonucleolytic cleavage of pre-tRNA, producing 5'-hydroxy and 2',3'-cyclic phosphate termini, and specifically removing the intron. HAMAP-Rule MF_01833
Subunit structure	Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers By similarity.
Sequence similarities	Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC tRNA splicing, via endonucleolytic cleavage and ligation Inferred from electronic annotation. Source: HAMAP
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro tRNA-intron endonuclease activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 170	170	tRNA-splicing endonuclease HAMAP-Rule MF_01833		PRO_0000109478
Sites
	Active site	110	1	By similarity
	Active site	116	1	By similarity
	Active site	147	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O58033 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: A03230ABFEDE2BDD	FASTA	170	20,168
	10 20 30 40 50 60 MKKPIEFYLS GDRVYSTREK AINQLYNNRG YGELKGDKLF LSLIEAAYLT EKGWIKVVDK 70 80 90 100 110 120 DKELKFDDLM KLGKSRDEDF DIKYIVYKDL RDRGYIVKSA LKFGSHYRVY RKDAEHSDWL 130 140 150 160 170 IWVLRENQRL SPNDITARAR VAHGVRKNMV LAIVDEDGDV VYYKVEWIKF

References

[1]

"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3." Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H. DNA Res. 5:55-76(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000001 Genomic DNA. Translation: BAA29367.1.
PIR	H71454.
RefSeq	NP_142282.1. NC_000961.1.
3D structure databases
ProteinModelPortal	O58033.
ModBase	Search...
Protein-protein interaction databases
STRING	70601.PH0295.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAA29367; BAA29367; BAA29367.
GeneID	1444177.
KEGG	pho:PH0295.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1676.
HOGENOM	HOG000107823.
KO	K01170.
OMA	FVRVAHS.
ProtClustDB	PRK09297.
Enzyme and pathway databases
BioCyc	PHOR70601:GJWR-278-MONOMER.
Family and domain databases
Gene3D	3.40.1350.10. 1 hit.
HAMAP	MF_01833. EndA_short.
InterPro	IPR011856. tRNA_endonuc-like_dom. IPR006677. tRNA_intron_Endonuc_cat-like. IPR006678. tRNA_intron_Endonuc_N. IPR006676. tRNA_splic. IPR016442. tRNA_splic_arch_short. [Graphical view]
Pfam	PF01974. tRNA_int_endo. 1 hit. PF02778. tRNA_int_endo_N. 1 hit. [Graphical view]
PIRSF	PIRSF005285. tRNA_splic_archaea. 1 hit.
SUPFAM	SSF53032. tRNA_int_endo_C. 1 hit. SSF55267. tRNA_int_endo_N. 1 hit.
TIGRFAMs	TIGR00324. endA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ENDA_PYRHO

Accession

Primary (citable) accession number: O58033

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	August 1, 1998
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program