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Protein

tRNA-splicing endonuclease

Gene

endA

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.UniRule annotation

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei110UniRule annotation1
Active sitei116UniRule annotation1
Active sitei147UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processtRNA processing

Enzyme and pathway databases

BioCyciPHOR70601:G1G39-276-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonucleaseUniRule annotation (EC:4.6.1.16UniRule annotation)
Alternative name(s):
tRNA-intron endonucleaseUniRule annotation
Gene namesi
Name:endAUniRule annotation
Ordered Locus Names:PH0295
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001094781 – 170tRNA-splicing endonucleaseAdd BLAST170

Interactioni

Subunit structurei

Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi70601.PH0295

Structurei

3D structure databases

ProteinModelPortaliO58033
SMRiO58033
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01701 Archaea
COG1676 LUCA
HOGENOMiHOG000107823
KOiK01170
OMAiKYGSDFR
OrthoDBiPOG093Z0DMC

Family and domain databases

Gene3Di3.40.1350.10, 1 hit
HAMAPiMF_01833 EndA_short, 1 hit
InterProiView protein in InterPro
IPR011856 tRNA_endonuc-like_dom_sf
IPR036167 tRNA_intron_Endo_cat-like_sf
IPR006677 tRNA_intron_Endonuc_cat-like
IPR006678 tRNA_intron_Endonuc_N
IPR036740 tRNA_intron_Endonuc_N_sf
IPR006676 tRNA_splic
IPR016442 tRNA_splic_arch_short
PANTHERiPTHR21227 PTHR21227, 2 hits
PfamiView protein in Pfam
PF01974 tRNA_int_endo, 1 hit
PF02778 tRNA_int_endo_N, 1 hit
PIRSFiPIRSF005285 tRNA_splic_archaea, 1 hit
SUPFAMiSSF53032 SSF53032, 1 hit
SSF55267 SSF55267, 1 hit
TIGRFAMsiTIGR00324 endA, 1 hit

Sequencei

Sequence statusi: Complete.

O58033-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKPIEFYLS GDRVYSTREK AINQLYNNRG YGELKGDKLF LSLIEAAYLT
60 70 80 90 100
EKGWIKVVDK DKELKFDDLM KLGKSRDEDF DIKYIVYKDL RDRGYIVKSA
110 120 130 140 150
LKFGSHYRVY RKDAEHSDWL IWVLRENQRL SPNDITARAR VAHGVRKNMV
160 170
LAIVDEDGDV VYYKVEWIKF
Length:170
Mass (Da):20,168
Last modified:August 1, 1998 - v1
Checksum:iA03230ABFEDE2BDD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA Translation: BAA29367.1
PIRiH71454
RefSeqiWP_010884390.1, NC_000961.1

Genome annotation databases

EnsemblBacteriaiBAA29367; BAA29367; BAA29367
GeneIDi1444177
KEGGipho:PH0295

Similar proteinsi

Entry informationi

Entry nameiENDA_PYRHO
AccessioniPrimary (citable) accession number: O58033
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: August 1, 1998
Last modified: April 25, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health