ID RADA_PYRHO Reviewed; 529 AA. AC O58001; DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot. DT 01-AUG-1998, sequence version 1. DT 27-MAR-2024, entry version 154. DE RecName: Full=DNA repair and recombination protein RadA; DE Contains: DE RecName: Full=Pho RadA intein; GN Name=radA; OrderedLocusNames=PH0263; OS Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC OS 100139 / OT-3). OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae; OC Pyrococcus. OX NCBI_TaxID=70601; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3; RX PubMed=9679194; DOI=10.1093/dnares/5.2.55; RA Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., RA Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., RA Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T., Tanaka T., RA Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., RA Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.; RT "Complete sequence and gene organization of the genome of a hyper- RT thermophilic archaebacterium, Pyrococcus horikoshii OT3."; RL DNA Res. 5:55-76(1998). CC -!- FUNCTION: Involved in DNA repair and in homologous recombination. Binds CC and assemble on single-stranded DNA to form a nucleoprotein filament. CC Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand CC exchange between homologous DNA molecules (By similarity). CC {ECO:0000250}. CC -!- PTM: This protein undergoes a protein self splicing that involves a CC post-translational excision of the intervening region (intein) followed CC by peptide ligation. {ECO:0000305}. CC -!- MISCELLANEOUS: The intein interrupts the ATP-binding site. CC -!- SIMILARITY: Belongs to the eukaryotic RecA-like protein family. CC {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; BA000001; BAA29335.1; -; Genomic_DNA. DR PIR; H71450; H71450. DR PDB; 2LQM; NMR; -; A=153-324. DR PDB; 4E2T; X-ray; 1.75 A; A/B=153-324. DR PDB; 4E2U; X-ray; 1.58 A; A=151-324. DR PDBsum; 2LQM; -. DR PDBsum; 4E2T; -. DR PDBsum; 4E2U; -. DR AlphaFoldDB; O58001; -. DR BMRB; O58001; -. DR SMR; O58001; -. DR STRING; 70601.gene:9377179; -. DR EnsemblBacteria; BAA29335; BAA29335; BAA29335. DR KEGG; pho:PH0263; -. DR eggNOG; arCOG00415; Archaea. DR BRENDA; 3.6.4.B7; 5244. DR Proteomes; UP000000752; Chromosome. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule. DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro. DR GO; GO:0003684; F:damaged DNA binding; IEA:UniProtKB-UniRule. DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule. DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule. DR GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro. DR CDD; cd19515; archRadA; 1. DR CDD; cd00081; Hint; 1. DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1. DR Gene3D; 2.170.16.10; Hedgehog/Intein (Hint) domain; 1. DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2. DR HAMAP; MF_00348; RadA_arch; 1. DR InterPro; IPR013632; DNA_recomb/repair_Rad51_C. DR InterPro; IPR011938; DNA_recomb/repair_RadA. DR InterPro; IPR010995; DNA_repair_Rad51/TF_NusA_a-hlx. DR InterPro; IPR003586; Hint_dom_C. DR InterPro; IPR003587; Hint_dom_N. DR InterPro; IPR036844; Hint_dom_sf. DR InterPro; IPR003583; Hlx-hairpin-Hlx_DNA-bd_motif. DR InterPro; IPR030934; Intein_C. DR InterPro; IPR006141; Intein_N. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR020588; RecA_ATP-bd. DR InterPro; IPR020587; RecA_monomer-monomer_interface. DR NCBIfam; TIGR01443; intein_Cterm; 1. DR NCBIfam; TIGR01445; intein_Nterm; 1. DR NCBIfam; TIGR02236; recomb_radA; 1. DR PANTHER; PTHR22942:SF39; DNA REPAIR PROTEIN RAD51 HOMOLOG 1; 1. DR PANTHER; PTHR22942; RECA/RAD51/RADA DNA STRAND-PAIRING FAMILY MEMBER; 1. DR Pfam; PF14520; HHH_5; 1. DR Pfam; PF14890; Intein_splicing; 1. DR Pfam; PF08423; Rad51; 2. DR SMART; SM00278; HhH1; 2. DR SMART; SM00305; HintC; 1. DR SMART; SM00306; HintN; 1. DR SUPFAM; SSF51294; Hedgehog/intein (Hint) domain; 1. DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2. DR SUPFAM; SSF47794; Rad51 N-terminal domain-like; 1. DR PROSITE; PS50818; INTEIN_C_TER; 1. DR PROSITE; PS50817; INTEIN_N_TER; 1. DR PROSITE; PS50162; RECA_2; 2. DR PROSITE; PS50163; RECA_3; 1. PE 1: Evidence at protein level; KW 3D-structure; ATP-binding; Autocatalytic cleavage; DNA damage; KW DNA recombination; DNA-binding; Nucleotide-binding; Protein splicing. FT CHAIN 1..152 FT /note="DNA repair and recombination protein RadA, 1st part" FT /evidence="ECO:0000255" FT /id="PRO_0000030161" FT CHAIN 153..324 FT /note="Pho RadA intein" FT /evidence="ECO:0000255" FT /id="PRO_0000030162" FT CHAIN 325..529 FT /note="DNA repair and recombination protein RadA, 2nd part" FT /evidence="ECO:0000255" FT /id="PRO_0000030163" FT STRAND 159..164 FT /evidence="ECO:0007829|PDB:4E2U" FT STRAND 167..172 FT /evidence="ECO:0007829|PDB:4E2U" FT HELIX 173..184 FT /evidence="ECO:0007829|PDB:4E2U" FT STRAND 187..189 FT /evidence="ECO:0007829|PDB:4E2U" FT STRAND 192..196 FT /evidence="ECO:0007829|PDB:4E2U" FT STRAND 198..205 FT /evidence="ECO:0007829|PDB:4E2U" FT TURN 207..209 FT /evidence="ECO:0007829|PDB:4E2U" FT STRAND 212..216 FT /evidence="ECO:0007829|PDB:4E2U" FT STRAND 219..233 FT /evidence="ECO:0007829|PDB:4E2U" FT STRAND 238..241 FT /evidence="ECO:0007829|PDB:4E2U" FT STRAND 246..258 FT /evidence="ECO:0007829|PDB:4E2U" FT HELIX 259..261 FT /evidence="ECO:0007829|PDB:4E2U" FT STRAND 267..272 FT /evidence="ECO:0007829|PDB:4E2U" FT HELIX 273..277 FT /evidence="ECO:0007829|PDB:4E2T" FT STRAND 278..280 FT /evidence="ECO:0007829|PDB:4E2T" FT STRAND 285..307 FT /evidence="ECO:0007829|PDB:4E2U" FT TURN 309..311 FT /evidence="ECO:0007829|PDB:4E2U" FT STRAND 312..315 FT /evidence="ECO:0007829|PDB:4E2U" FT STRAND 320..323 FT /evidence="ECO:0007829|PDB:4E2U" SQ SEQUENCE 529 AA; 58949 MW; 1003B397D1A51567 CRC64; MMIMVKKGSD PEVVEIDEIE GLGLELEEES TTSKKKKKEK EIKSIEDLPG VGPATAEKLR EAGFDTLEAI AVASPIELKE VAGISEGAAL KIIQAARKAA NLGTFMRADE YLKKRESIGR ISTGSKSLDK LLGGGIETQA ITEVFGEFGS GKCFARDTEV YYENDTVPHM ESIEEMYSKY ASMNGELPFD NGYAVPLDNV FVYTLDIASG EIKKTRASYI YREKVEKLIE IKLSSGYSLK VTPSHPVLLF RDGLQWVPAA EVKPGDVVVG VREEVLRRRI ISKGELEFHE VSSVRIIDYN NWVYDLVIPE THNFIAPNGL VLHNTQLAHT LAVMVQLPPE EGGLNGSVIW IDTENTFRPE RIREIAKNRG LDPDEVLKHI YVARAFNSNH QMLLVQQAED KIKELLNTDK PVKLLIVDSL TSHFRSEYIG RGALAERQQK LAKHLADLHR LANLYEIAVF VTNQVQARPD AFFGDPTRPI GGHILAHSAT LRVYLRKGKG GKRVARLIDA PHLPEGEAVF RITEKGIED //