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O58001 (RADA_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA repair and recombination protein RadA

Cleaved into the following chain:

  1. Pho RadA intein
Gene names
Name:radA
Ordered Locus Names:PH0263
OrganismPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]
Taxonomic identifier70601 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length529 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules By similarity. HAMAP-Rule MF_00348

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential. HAMAP-Rule MF_00348

Miscellaneous

The intein interrupts the ATP-binding site.

Sequence similarities

Belongs to the eukaryotic RecA-like protein family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 152152DNA repair and recombination protein RadA, 1st part Potential
PRO_0000030161
Chain153 – 324172Pho RadA intein Potential
PRO_0000030162
Chain325 – 529205DNA repair and recombination protein RadA, 2nd part Potential
PRO_0000030163

Secondary structure

.................................. 529
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O58001 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 1003B397D1A51567

FASTA52958,949
        10         20         30         40         50         60 
MMIMVKKGSD PEVVEIDEIE GLGLELEEES TTSKKKKKEK EIKSIEDLPG VGPATAEKLR 

        70         80         90        100        110        120 
EAGFDTLEAI AVASPIELKE VAGISEGAAL KIIQAARKAA NLGTFMRADE YLKKRESIGR 

       130        140        150        160        170        180 
ISTGSKSLDK LLGGGIETQA ITEVFGEFGS GKCFARDTEV YYENDTVPHM ESIEEMYSKY 

       190        200        210        220        230        240 
ASMNGELPFD NGYAVPLDNV FVYTLDIASG EIKKTRASYI YREKVEKLIE IKLSSGYSLK 

       250        260        270        280        290        300 
VTPSHPVLLF RDGLQWVPAA EVKPGDVVVG VREEVLRRRI ISKGELEFHE VSSVRIIDYN 

       310        320        330        340        350        360 
NWVYDLVIPE THNFIAPNGL VLHNTQLAHT LAVMVQLPPE EGGLNGSVIW IDTENTFRPE 

       370        380        390        400        410        420 
RIREIAKNRG LDPDEVLKHI YVARAFNSNH QMLLVQQAED KIKELLNTDK PVKLLIVDSL 

       430        440        450        460        470        480 
TSHFRSEYIG RGALAERQQK LAKHLADLHR LANLYEIAVF VTNQVQARPD AFFGDPTRPI 

       490        500        510        520 
GGHILAHSAT LRVYLRKGKG GKRVARLIDA PHLPEGEAVF RITEKGIED 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000001 Genomic DNA. Translation: BAA29335.1.
PIRH71450.
RefSeqNP_142255.1. NC_000961.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2LQMNMR-A154-324[»]
4E2TX-ray1.75A/B154-324[»]
4E2UX-ray1.58A154-324[»]
ProteinModelPortalO58001.
SMRO58001. Positions 43-152, 325-529.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING70601.PH0263.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA29335; BAA29335; BAA29335.
GeneID1444149.
KEGGpho:PH0263.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1372.
HOGENOMHOG000227426.
KOK04483.
OMASGKCFAK.
ProtClustDBCLSK2561163.

Enzyme and pathway databases

BioCycPHOR70601:GJWR-250-MONOMER.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
HAMAPMF_00348. RadA.
InterProIPR013632. DNA_recomb/repair_Rad51_C.
IPR011938. DNA_recomb/repair_RadA.
IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]
PfamPF08423. Rad51. 2 hits.
[Graphical view]
SMARTSM00278. HhH1. 2 hits.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMSSF47794. SSF47794. 1 hit.
SSF52540. SSF52540. 2 hits.
TIGRFAMsTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR02236. recomb_radA. 1 hit.
PROSITEPS50818. INTEIN_C_TER. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS50162. RECA_2. 2 hits.
PS50163. RECA_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRADA_PYRHO
AccessionPrimary (citable) accession number: O58001
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: August 1, 1998
Last modified: April 16, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Intein-containing proteins

List of intein-containing protein entries