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O58001

- RADA_PYRHO

UniProt

O58001 - RADA_PYRHO

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Protein

DNA repair and recombination protein RadA

Gene

radA

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules (By similarity).By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. damaged DNA binding Source: UniProtKB-HAMAP
  3. DNA-dependent ATPase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA recombination Source: UniProtKB-HAMAP
  2. DNA repair Source: UniProtKB-HAMAP
  3. intein-mediated protein splicing Source: InterPro
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA recombination

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-250-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair and recombination protein RadA
Cleaved into the following chain:
Gene namesi
Name:radA
Ordered Locus Names:PH0263
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000000752: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 152152DNA repair and recombination protein RadA, 1st partSequence AnalysisPRO_0000030161Add
BLAST
Chaini153 – 324172Pho RadA inteinSequence AnalysisPRO_0000030162Add
BLAST
Chaini325 – 529205DNA repair and recombination protein RadA, 2nd partSequence AnalysisPRO_0000030163Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Protein-protein interaction databases

STRINGi70601.PH0263.

Structurei

Secondary structure

1
529
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi159 – 1646Combined sources
Beta strandi167 – 1726Combined sources
Helixi173 – 18412Combined sources
Beta strandi187 – 1893Combined sources
Beta strandi192 – 1965Combined sources
Beta strandi198 – 2058Combined sources
Turni207 – 2093Combined sources
Beta strandi212 – 2165Combined sources
Beta strandi219 – 23315Combined sources
Beta strandi238 – 2414Combined sources
Beta strandi246 – 25813Combined sources
Helixi259 – 2613Combined sources
Beta strandi267 – 2726Combined sources
Helixi273 – 2775Combined sources
Beta strandi278 – 2803Combined sources
Beta strandi285 – 30723Combined sources
Turni309 – 3113Combined sources
Beta strandi312 – 3154Combined sources
Beta strandi320 – 3234Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LQMNMR-A153-324[»]
4E2TX-ray1.75A/B153-324[»]
4E2UX-ray1.58A151-324[»]
ProteinModelPortaliO58001.
SMRiO58001. Positions 43-152, 325-529.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1372.
HOGENOMiHOG000227426.
KOiK04483.
OMAiSGKCFAK.

Family and domain databases

Gene3Di2.170.16.10. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00348. RadA.
InterProiIPR013632. DNA_recomb/repair_Rad51_C.
IPR011938. DNA_recomb/repair_RadA.
IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]
PfamiPF08423. Rad51. 2 hits.
[Graphical view]
SMARTiSM00278. HhH1. 2 hits.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF47794. SSF47794. 1 hit.
SSF51294. SSF51294. 1 hit.
SSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR02236. recomb_radA. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS50162. RECA_2. 2 hits.
PS50163. RECA_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O58001-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMIMVKKGSD PEVVEIDEIE GLGLELEEES TTSKKKKKEK EIKSIEDLPG
60 70 80 90 100
VGPATAEKLR EAGFDTLEAI AVASPIELKE VAGISEGAAL KIIQAARKAA
110 120 130 140 150
NLGTFMRADE YLKKRESIGR ISTGSKSLDK LLGGGIETQA ITEVFGEFGS
160 170 180 190 200
GKCFARDTEV YYENDTVPHM ESIEEMYSKY ASMNGELPFD NGYAVPLDNV
210 220 230 240 250
FVYTLDIASG EIKKTRASYI YREKVEKLIE IKLSSGYSLK VTPSHPVLLF
260 270 280 290 300
RDGLQWVPAA EVKPGDVVVG VREEVLRRRI ISKGELEFHE VSSVRIIDYN
310 320 330 340 350
NWVYDLVIPE THNFIAPNGL VLHNTQLAHT LAVMVQLPPE EGGLNGSVIW
360 370 380 390 400
IDTENTFRPE RIREIAKNRG LDPDEVLKHI YVARAFNSNH QMLLVQQAED
410 420 430 440 450
KIKELLNTDK PVKLLIVDSL TSHFRSEYIG RGALAERQQK LAKHLADLHR
460 470 480 490 500
LANLYEIAVF VTNQVQARPD AFFGDPTRPI GGHILAHSAT LRVYLRKGKG
510 520
GKRVARLIDA PHLPEGEAVF RITEKGIED
Length:529
Mass (Da):58,949
Last modified:August 1, 1998 - v1
Checksum:i1003B397D1A51567
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29335.1.
PIRiH71450.
RefSeqiNP_142255.1. NC_000961.1.
WP_010884362.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA29335; BAA29335; BAA29335.
GeneIDi1444149.
KEGGipho:PH0263.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29335.1 .
PIRi H71450.
RefSeqi NP_142255.1. NC_000961.1.
WP_010884362.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2LQM NMR - A 153-324 [» ]
4E2T X-ray 1.75 A/B 153-324 [» ]
4E2U X-ray 1.58 A 151-324 [» ]
ProteinModelPortali O58001.
SMRi O58001. Positions 43-152, 325-529.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 70601.PH0263.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA29335 ; BAA29335 ; BAA29335 .
GeneIDi 1444149.
KEGGi pho:PH0263.

Phylogenomic databases

eggNOGi COG1372.
HOGENOMi HOG000227426.
KOi K04483.
OMAi SGKCFAK.

Enzyme and pathway databases

BioCyci PHOR70601:GJWR-250-MONOMER.

Family and domain databases

Gene3Di 2.170.16.10. 1 hit.
3.40.50.300. 2 hits.
HAMAPi MF_00348. RadA.
InterProi IPR013632. DNA_recomb/repair_Rad51_C.
IPR011938. DNA_recomb/repair_RadA.
IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view ]
Pfami PF08423. Rad51. 2 hits.
[Graphical view ]
SMARTi SM00278. HhH1. 2 hits.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view ]
SUPFAMi SSF47794. SSF47794. 1 hit.
SSF51294. SSF51294. 1 hit.
SSF52540. SSF52540. 2 hits.
TIGRFAMsi TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR02236. recomb_radA. 1 hit.
PROSITEi PS50818. INTEIN_C_TER. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS50162. RECA_2. 2 hits.
PS50163. RECA_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Entry informationi

Entry nameiRADA_PYRHO
AccessioniPrimary (citable) accession number: O58001
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: August 1, 1998
Last modified: November 26, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The intein interrupts the ATP-binding site.

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3