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O58001

- RADA_PYRHO

UniProt

O58001 - RADA_PYRHO

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Protein

DNA repair and recombination protein RadA

Gene
radA, PH0263
Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed - Annotation score: 2 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules By similarity.UniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. damaged DNA binding Source: UniProtKB-HAMAP
  3. DNA-dependent ATPase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA recombination Source: UniProtKB-HAMAP
  2. DNA repair Source: UniProtKB-HAMAP
  3. intein-mediated protein splicing Source: InterPro
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA recombination

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-250-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair and recombination protein RadA
Cleaved into the following chain:
Gene namesi
Name:radA
Ordered Locus Names:PH0263
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000000752: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 152152DNA repair and recombination protein RadA, 1st part Reviewed predictionPRO_0000030161Add
BLAST
Chaini153 – 324172Pho RadA intein Reviewed predictionPRO_0000030162Add
BLAST
Chaini325 – 529205DNA repair and recombination protein RadA, 2nd part Reviewed predictionPRO_0000030163Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Reviewed prediction.UniRule annotation

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Protein-protein interaction databases

STRINGi70601.PH0263.

Structurei

Secondary structure

1
529
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi159 – 1646
Beta strandi167 – 1726
Helixi173 – 18412
Beta strandi187 – 1893
Beta strandi192 – 1965
Beta strandi198 – 2058
Turni207 – 2093
Beta strandi212 – 2165
Beta strandi219 – 23315
Beta strandi238 – 2414
Beta strandi246 – 25813
Helixi259 – 2613
Beta strandi267 – 2726
Helixi273 – 2775
Beta strandi278 – 2803
Beta strandi285 – 30723
Turni309 – 3113
Beta strandi312 – 3154
Beta strandi320 – 3234

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LQMNMR-A153-324[»]
4E2TX-ray1.75A/B153-324[»]
4E2UX-ray1.58A151-324[»]
ProteinModelPortaliO58001.
SMRiO58001. Positions 43-152, 325-529.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1372.
HOGENOMiHOG000227426.
KOiK04483.
OMAiSGKCFAK.

Family and domain databases

Gene3Di2.170.16.10. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00348. RadA.
InterProiIPR013632. DNA_recomb/repair_Rad51_C.
IPR011938. DNA_recomb/repair_RadA.
IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]
PfamiPF08423. Rad51. 2 hits.
[Graphical view]
SMARTiSM00278. HhH1. 2 hits.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF47794. SSF47794. 1 hit.
SSF51294. SSF51294. 1 hit.
SSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR02236. recomb_radA. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS50162. RECA_2. 2 hits.
PS50163. RECA_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O58001-1 [UniParc]FASTAAdd to Basket

« Hide

MMIMVKKGSD PEVVEIDEIE GLGLELEEES TTSKKKKKEK EIKSIEDLPG    50
VGPATAEKLR EAGFDTLEAI AVASPIELKE VAGISEGAAL KIIQAARKAA 100
NLGTFMRADE YLKKRESIGR ISTGSKSLDK LLGGGIETQA ITEVFGEFGS 150
GKCFARDTEV YYENDTVPHM ESIEEMYSKY ASMNGELPFD NGYAVPLDNV 200
FVYTLDIASG EIKKTRASYI YREKVEKLIE IKLSSGYSLK VTPSHPVLLF 250
RDGLQWVPAA EVKPGDVVVG VREEVLRRRI ISKGELEFHE VSSVRIIDYN 300
NWVYDLVIPE THNFIAPNGL VLHNTQLAHT LAVMVQLPPE EGGLNGSVIW 350
IDTENTFRPE RIREIAKNRG LDPDEVLKHI YVARAFNSNH QMLLVQQAED 400
KIKELLNTDK PVKLLIVDSL TSHFRSEYIG RGALAERQQK LAKHLADLHR 450
LANLYEIAVF VTNQVQARPD AFFGDPTRPI GGHILAHSAT LRVYLRKGKG 500
GKRVARLIDA PHLPEGEAVF RITEKGIED 529
Length:529
Mass (Da):58,949
Last modified:August 1, 1998 - v1
Checksum:i1003B397D1A51567
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000001 Genomic DNA. Translation: BAA29335.1.
PIRiH71450.
RefSeqiNP_142255.1. NC_000961.1.
WP_010884362.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA29335; BAA29335; BAA29335.
GeneIDi1444149.
KEGGipho:PH0263.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000001 Genomic DNA. Translation: BAA29335.1 .
PIRi H71450.
RefSeqi NP_142255.1. NC_000961.1.
WP_010884362.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2LQM NMR - A 153-324 [» ]
4E2T X-ray 1.75 A/B 153-324 [» ]
4E2U X-ray 1.58 A 151-324 [» ]
ProteinModelPortali O58001.
SMRi O58001. Positions 43-152, 325-529.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 70601.PH0263.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA29335 ; BAA29335 ; BAA29335 .
GeneIDi 1444149.
KEGGi pho:PH0263.

Phylogenomic databases

eggNOGi COG1372.
HOGENOMi HOG000227426.
KOi K04483.
OMAi SGKCFAK.

Enzyme and pathway databases

BioCyci PHOR70601:GJWR-250-MONOMER.

Family and domain databases

Gene3Di 2.170.16.10. 1 hit.
3.40.50.300. 2 hits.
HAMAPi MF_00348. RadA.
InterProi IPR013632. DNA_recomb/repair_Rad51_C.
IPR011938. DNA_recomb/repair_RadA.
IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view ]
Pfami PF08423. Rad51. 2 hits.
[Graphical view ]
SMARTi SM00278. HhH1. 2 hits.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view ]
SUPFAMi SSF47794. SSF47794. 1 hit.
SSF51294. SSF51294. 1 hit.
SSF52540. SSF52540. 2 hits.
TIGRFAMsi TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR02236. recomb_radA. 1 hit.
PROSITEi PS50818. INTEIN_C_TER. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS50162. RECA_2. 2 hits.
PS50163. RECA_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Entry informationi

Entry nameiRADA_PYRHO
AccessioniPrimary (citable) accession number: O58001
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: August 1, 1998
Last modified: September 3, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The intein interrupts the ATP-binding site.

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi