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O58001

- RADA_PYRHO

UniProt

O58001 - RADA_PYRHO

Protein

DNA repair and recombination protein RadA

Gene

radA

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules By similarity.By similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. damaged DNA binding Source: UniProtKB-HAMAP
    3. DNA-dependent ATPase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA recombination Source: UniProtKB-HAMAP
    2. DNA repair Source: UniProtKB-HAMAP
    3. intein-mediated protein splicing Source: InterPro

    Keywords - Biological processi

    DNA damage, DNA recombination

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciPHOR70601:GJWR-250-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA repair and recombination protein RadA
    Cleaved into the following chain:
    Gene namesi
    Name:radA
    Ordered Locus Names:PH0263
    OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
    Taxonomic identifieri70601 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    ProteomesiUP000000752: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 152152DNA repair and recombination protein RadA, 1st partSequence AnalysisPRO_0000030161Add
    BLAST
    Chaini153 – 324172Pho RadA inteinSequence AnalysisPRO_0000030162Add
    BLAST
    Chaini325 – 529205DNA repair and recombination protein RadA, 2nd partSequence AnalysisPRO_0000030163Add
    BLAST

    Post-translational modificationi

    This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

    Keywords - PTMi

    Autocatalytic cleavage, Protein splicing

    Interactioni

    Protein-protein interaction databases

    STRINGi70601.PH0263.

    Structurei

    Secondary structure

    1
    529
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi159 – 1646
    Beta strandi167 – 1726
    Helixi173 – 18412
    Beta strandi187 – 1893
    Beta strandi192 – 1965
    Beta strandi198 – 2058
    Turni207 – 2093
    Beta strandi212 – 2165
    Beta strandi219 – 23315
    Beta strandi238 – 2414
    Beta strandi246 – 25813
    Helixi259 – 2613
    Beta strandi267 – 2726
    Helixi273 – 2775
    Beta strandi278 – 2803
    Beta strandi285 – 30723
    Turni309 – 3113
    Beta strandi312 – 3154
    Beta strandi320 – 3234

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2LQMNMR-A153-324[»]
    4E2TX-ray1.75A/B153-324[»]
    4E2UX-ray1.58A151-324[»]
    ProteinModelPortaliO58001.
    SMRiO58001. Positions 43-152, 325-529.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1372.
    HOGENOMiHOG000227426.
    KOiK04483.
    OMAiSGKCFAK.

    Family and domain databases

    Gene3Di2.170.16.10. 1 hit.
    3.40.50.300. 2 hits.
    HAMAPiMF_00348. RadA.
    InterProiIPR013632. DNA_recomb/repair_Rad51_C.
    IPR011938. DNA_recomb/repair_RadA.
    IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
    IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
    IPR006141. Intein_splice_site.
    IPR027417. P-loop_NTPase.
    IPR020588. RecA_ATP-bd.
    IPR020587. RecA_monomer-monomer_interface.
    [Graphical view]
    PfamiPF08423. Rad51. 2 hits.
    [Graphical view]
    SMARTiSM00278. HhH1. 2 hits.
    SM00305. HintC. 1 hit.
    SM00306. HintN. 1 hit.
    [Graphical view]
    SUPFAMiSSF47794. SSF47794. 1 hit.
    SSF51294. SSF51294. 1 hit.
    SSF52540. SSF52540. 2 hits.
    TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
    TIGR01445. intein_Nterm. 1 hit.
    TIGR02236. recomb_radA. 1 hit.
    PROSITEiPS50818. INTEIN_C_TER. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    PS50162. RECA_2. 2 hits.
    PS50163. RECA_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O58001-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMIMVKKGSD PEVVEIDEIE GLGLELEEES TTSKKKKKEK EIKSIEDLPG    50
    VGPATAEKLR EAGFDTLEAI AVASPIELKE VAGISEGAAL KIIQAARKAA 100
    NLGTFMRADE YLKKRESIGR ISTGSKSLDK LLGGGIETQA ITEVFGEFGS 150
    GKCFARDTEV YYENDTVPHM ESIEEMYSKY ASMNGELPFD NGYAVPLDNV 200
    FVYTLDIASG EIKKTRASYI YREKVEKLIE IKLSSGYSLK VTPSHPVLLF 250
    RDGLQWVPAA EVKPGDVVVG VREEVLRRRI ISKGELEFHE VSSVRIIDYN 300
    NWVYDLVIPE THNFIAPNGL VLHNTQLAHT LAVMVQLPPE EGGLNGSVIW 350
    IDTENTFRPE RIREIAKNRG LDPDEVLKHI YVARAFNSNH QMLLVQQAED 400
    KIKELLNTDK PVKLLIVDSL TSHFRSEYIG RGALAERQQK LAKHLADLHR 450
    LANLYEIAVF VTNQVQARPD AFFGDPTRPI GGHILAHSAT LRVYLRKGKG 500
    GKRVARLIDA PHLPEGEAVF RITEKGIED 529
    Length:529
    Mass (Da):58,949
    Last modified:August 1, 1998 - v1
    Checksum:i1003B397D1A51567
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000001 Genomic DNA. Translation: BAA29335.1.
    PIRiH71450.
    RefSeqiNP_142255.1. NC_000961.1.
    WP_010884362.1. NC_000961.1.

    Genome annotation databases

    EnsemblBacteriaiBAA29335; BAA29335; BAA29335.
    GeneIDi1444149.
    KEGGipho:PH0263.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000001 Genomic DNA. Translation: BAA29335.1 .
    PIRi H71450.
    RefSeqi NP_142255.1. NC_000961.1.
    WP_010884362.1. NC_000961.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2LQM NMR - A 153-324 [» ]
    4E2T X-ray 1.75 A/B 153-324 [» ]
    4E2U X-ray 1.58 A 151-324 [» ]
    ProteinModelPortali O58001.
    SMRi O58001. Positions 43-152, 325-529.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 70601.PH0263.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAA29335 ; BAA29335 ; BAA29335 .
    GeneIDi 1444149.
    KEGGi pho:PH0263.

    Phylogenomic databases

    eggNOGi COG1372.
    HOGENOMi HOG000227426.
    KOi K04483.
    OMAi SGKCFAK.

    Enzyme and pathway databases

    BioCyci PHOR70601:GJWR-250-MONOMER.

    Family and domain databases

    Gene3Di 2.170.16.10. 1 hit.
    3.40.50.300. 2 hits.
    HAMAPi MF_00348. RadA.
    InterProi IPR013632. DNA_recomb/repair_Rad51_C.
    IPR011938. DNA_recomb/repair_RadA.
    IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
    IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
    IPR006141. Intein_splice_site.
    IPR027417. P-loop_NTPase.
    IPR020588. RecA_ATP-bd.
    IPR020587. RecA_monomer-monomer_interface.
    [Graphical view ]
    Pfami PF08423. Rad51. 2 hits.
    [Graphical view ]
    SMARTi SM00278. HhH1. 2 hits.
    SM00305. HintC. 1 hit.
    SM00306. HintN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47794. SSF47794. 1 hit.
    SSF51294. SSF51294. 1 hit.
    SSF52540. SSF52540. 2 hits.
    TIGRFAMsi TIGR01443. intein_Cterm. 1 hit.
    TIGR01445. intein_Nterm. 1 hit.
    TIGR02236. recomb_radA. 1 hit.
    PROSITEi PS50818. INTEIN_C_TER. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    PS50162. RECA_2. 2 hits.
    PS50163. RECA_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

    Entry informationi

    Entry nameiRADA_PYRHO
    AccessioniPrimary (citable) accession number: O58001
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 27, 2001
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The intein interrupts the ATP-binding site.

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. Intein-containing proteins
      List of intein-containing protein entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3