O58001 (RADA_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA repair and recombination protein radA

Cleaved into the following chain:

1. Pho radA intein

Gene names

Name:	radA
Ordered Locus Names:	PH0263

Organism

Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]

Taxonomic identifier

70601 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	529 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules By similarity. HAMAP MF_00348
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential. HAMAP MF_00348
Miscellaneous	The intein interrupts the ATP-binding site. HAMAP MF_00348
Sequence similarities	Belongs to the eukaryotic recA-like protein family.

Ontologies

Keywords
Biological process	DNA damage DNA recombination
Ligand	ATP-binding DNA-binding Nucleotide-binding
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA repair Inferred from electronic annotation. Source: InterPro intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-dependent ATPase activity Inferred from electronic annotation. Source: InterPro damaged DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 152	152	DNA repair and recombination protein radA, 1st part Potential		PRO_0000030161
	Chain	153 – 324	172	Pho radA intein Potential		PRO_0000030162
	Chain	325 – 529	205	DNA repair and recombination protein radA, 2nd part Potential		PRO_0000030163

Sequences

	Sequence		Length	Mass (Da)
	O58001 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 1003B397D1A51567	FASTA	529	58,949
	10 20 30 40 50 60 MMIMVKKGSD PEVVEIDEIE GLGLELEEES TTSKKKKKEK EIKSIEDLPG VGPATAEKLR 70 80 90 100 110 120 EAGFDTLEAI AVASPIELKE VAGISEGAAL KIIQAARKAA NLGTFMRADE YLKKRESIGR 130 140 150 160 170 180 ISTGSKSLDK LLGGGIETQA ITEVFGEFGS GKCFARDTEV YYENDTVPHM ESIEEMYSKY 190 200 210 220 230 240 ASMNGELPFD NGYAVPLDNV FVYTLDIASG EIKKTRASYI YREKVEKLIE IKLSSGYSLK 250 260 270 280 290 300 VTPSHPVLLF RDGLQWVPAA EVKPGDVVVG VREEVLRRRI ISKGELEFHE VSSVRIIDYN 310 320 330 340 350 360 NWVYDLVIPE THNFIAPNGL VLHNTQLAHT LAVMVQLPPE EGGLNGSVIW IDTENTFRPE 370 380 390 400 410 420 RIREIAKNRG LDPDEVLKHI YVARAFNSNH QMLLVQQAED KIKELLNTDK PVKLLIVDSL 430 440 450 460 470 480 TSHFRSEYIG RGALAERQQK LAKHLADLHR LANLYEIAVF VTNQVQARPD AFFGDPTRPI 490 500 510 520 GGHILAHSAT LRVYLRKGKG GKRVARLIDA PHLPEGEAVF RITEKGIED

References

[1]

"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3." Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H. DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000001 Genomic DNA. Translation: BAA29335.1.
PIR	H71450.
RefSeq	NP_142255.1. NC_000961.1.
3D structure databases
ProteinModelPortal	O58001.
SMR	O58001. Positions 43-152, 325-529.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000000605; EBPYRP00000000605; EBPYRG00000000605.
GeneID	1444149.
GenomeReviews	Gene locus PH0263 in contig BA000001_GR.
KEGG	pho:PH0263.
NMPDR	fig|70601.1.peg.249.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000022155.
HOGENOM	HBG413235.
OMA	MESIEEM.
PhylomeDB	O58001.
ProtClustDB	CLSK2561163.
Enzyme and pathway databases
BioCyc	PHOR70601:PH0263-MONOMER.
Family and domain databases
HAMAP	MF_00348. RadA. [Tree]
InterPro	IPR013632. DNA_recomb/repair_Rad51_C. IPR011938. DNA_recomb/repair_RadA. IPR020588. DNA_recomb_RecA/RadB_ATP-bd. IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx. IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif. IPR006141. Intein_splice_site. IPR020587. RecA_monomer-monomer_interface. [Graphical view]
KO	K04483.
PANTHER	PTHR22942:SF11. PTHR22942:SF11. 1 hit.
Pfam	PF08423. Rad51. 2 hits. [Graphical view]
SMART	SM00278. HhH1. 2 hits. SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
SUPFAM	SSF47794. Rad51_N. 1 hit.
TIGRFAMs	TIGR01443. Intein_Cterm. 1 hit. TIGR01445. Intein_Nterm. 1 hit. TIGR02236. Recomb_radA. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50817. INTEIN_N_TER. 1 hit. PS50162. RECA_2. 2 hits. PS50163. RECA_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RADA_PYRHO

Accession

Primary (citable) accession number: O58001

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	August 1, 1998
Last modified:	December 14, 2011
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program