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O57981 (GLMS_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]

EC=2.6.1.16
Alternative name(s):
D-fructose-6-phosphate amidotransferase
GFAT
Glucosamine-6-phosphate synthase
Hexosephosphate aminotransferase
L-glutamine--D-fructose-6-phosphate amidotransferase
Gene names
Name:glmS
Ordered Locus Names:PH0243
OrganismPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]
Taxonomic identifier70601 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length598 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source By similarity. HAMAP-Rule MF_00164

Catalytic activity

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate. HAMAP-Rule MF_00164

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00164

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00164.

Sequence similarities

Contains 1 glutamine amidotransferase type-2 domain.

Contains 2 SIS domains.

Sequence caution

The sequence BAA29315.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 598597Glutamine--fructose-6-phosphate aminotransferase [isomerizing] HAMAP-Rule MF_00164
PRO_0000135431

Regions

Domain2 – 219218Glutamine amidotransferase type-2
Domain280 – 420141SIS 1
Domain449 – 588140SIS 2

Sites

Active site21Nucleophile; for GATase activity By similarity
Active site5931For Fru-6P isomerization activity By similarity

Sequences

Sequence LengthMass (Da)Tools
O57981 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 3A8DD0466416544D

FASTA59866,110
        10         20         30         40         50         60 
MCGIIGYIGP RKASDVIVEG LKRLEYRGYD SAGIATCYEG KIFIKKGAGK IDELVKKLNF 

        70         80         90        100        110        120 
LELPGNIGIG HTRWATHGIP NDTNAHPHTD CTGKIVVVHN GIIENFQELK RELLKRGHVF 

       130        140        150        160        170        180 
RSDTDTEVIA HLIEENLRIT GNFEDAFRMS LLRLRGSYAL VVLFADDPER LYIARKDSPL 

       190        200        210        220        230        240 
IIGIGKGEMF MASDIPAFLA YTRRAVFLDD GEYGIVSKDW FTIKDIITGA VKTKEIHEIQ 

       250        260        270        280        290        300 
WTLEMAEKGG YEHFMLKEIF EQPKAIKDAI YGNVKEAPKV AELLMKYDRI IITGMGTSYH 

       310        320        330        340        350        360 
AALVGKYLIQ RFGKVPVIVE EASELRYEYE DILDNRSLLI AITQSGETAD TVAAMKLAKS 

       370        380        390        400        410        420 
KGVEVVGIVN VVGSLATRIA DETLYTHAGP EIGVAATKTY TTQLVVLTLL AKELGKLVGI 

       430        440        450        460        470        480 
DVSQIEGTIP RLPELVDSSL KINDKIREIA VKLNDKRDFF YIGRGINYPT ALEGALKIKE 

       490        500        510        520        530        540 
IAYVHAEGLS AGELKHGPLA LIEDGIPVVG IAPTGKTFEK MLSNIEEAKA RGGFIISLGD 

       550        560        570        580        590 
DVRLHQVSDI FIRLPKVPEE LAPITYIVPL QLLAYHLAVL KGHNPDRPRN LAKSVTVE 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000001 Genomic DNA. Translation: BAA29315.1. Different initiation.
PIRD71248.
RefSeqNP_142239.1. NC_000961.1.

3D structure databases

ProteinModelPortalO57981.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING70601.PH0243.

Protein family/group databases

MEROPSC44.971.

Proteomic databases

PRIDEO57981.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA29315; BAA29315; BAA29315.
GeneID1444133.
KEGGpho:PH0243.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0449.
HOGENOMHOG000258898.
KOK00820.
OMAFYTHAGP.

Enzyme and pathway databases

BioCycPHOR70601:GJWR-234-MONOMER.

Family and domain databases

Gene3D3.60.20.10. 1 hit.
HAMAPMF_00164. GlmS.
InterProIPR017932. GATase_2_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PANTHERPTHR10937:SF0. PTHR10937:SF0. 1 hit.
PfamPF01380. SIS. 2 hits.
[Graphical view]
SUPFAMSSF56235. SSF56235. 1 hit.
TIGRFAMsTIGR01135. glmS. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLMS_PYRHO
AccessionPrimary (citable) accession number: O57981
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 94 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families