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Protein

Asparagine--tRNA ligase

Gene

asnS

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-232-MONOMER.
BRENDAi6.1.1.22. 5244.

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine--tRNA ligaseUniRule annotation (EC:6.1.1.22UniRule annotation)
Alternative name(s):
Asparaginyl-tRNA synthetaseUniRule annotation
Short name:
AsnRSUniRule annotation
Gene namesi
Name:asnSUniRule annotation
Ordered Locus Names:PH0241
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 434434Asparagine--tRNA ligasePRO_0000176487Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi70601.PH0241.

Structurei

Secondary structure

1
434
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 93Combined sources
Helixi12 – 143Combined sources
Beta strandi18 – 3114Combined sources
Beta strandi34 – 418Combined sources
Beta strandi44 – 507Combined sources
Helixi52 – 554Combined sources
Helixi57 – 648Combined sources
Beta strandi71 – 8010Combined sources
Helixi85 – 873Combined sources
Beta strandi88 – 9912Combined sources
Helixi110 – 1123Combined sources
Helixi115 – 1206Combined sources
Helixi122 – 1254Combined sources
Helixi129 – 15123Combined sources
Beta strandi161 – 1644Combined sources
Helixi170 – 1723Combined sources
Beta strandi175 – 1784Combined sources
Beta strandi181 – 1855Combined sources
Helixi190 – 20011Combined sources
Beta strandi201 – 21010Combined sources
Beta strandi222 – 23211Combined sources
Helixi236 – 25722Combined sources
Helixi259 – 2635Combined sources
Helixi270 – 2734Combined sources
Beta strandi281 – 2833Combined sources
Helixi284 – 29310Combined sources
Helixi306 – 3138Combined sources
Beta strandi320 – 3267Combined sources
Helixi327 – 3293Combined sources
Beta strandi343 – 3519Combined sources
Turni352 – 3554Combined sources
Beta strandi356 – 3649Combined sources
Helixi368 – 37710Combined sources
Helixi382 – 3854Combined sources
Helixi386 – 3905Combined sources
Turni391 – 3933Combined sources
Beta strandi399 – 4057Combined sources
Helixi406 – 4138Combined sources
Helixi419 – 4224Combined sources
Beta strandi423 – 4253Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1X54X-ray1.45A1-434[»]
1X55X-ray1.80A1-434[»]
1X56X-ray1.98A1-434[»]
ProteinModelPortaliO57980.
SMRiO57980. Positions 1-434.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO57980.

Family & Domainsi

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00407. Archaea.
COG0017. LUCA.
HOGENOMiHOG000226034.
KOiK01893.
OMAiWGDDIGA.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00534. Asn_tRNA_synth. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004522. Asn-tRNA-ligase.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF16. PTHR22594:SF16. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00457. asnS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O57980-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEKVYCQEV KPELDGKKVR LAGWVYTNMR VGKKIFLWIR DSTGIVQAVV
60 70 80 90 100
AKNVVGEETF EKAKKLGRES SVIVEGIVKA DERAPGGAEV HVEKLEVIQA
110 120 130 140 150
VSEFPIPENP EQASPELLLD YRHLHIRTPK ASAIMKVKET LIMAAREWLL
160 170 180 190 200
KDGWHEVFPP ILVTGAVEGG ATLFKLKYFD KYAYLSQSAQ LYLEAAIFGL
210 220 230 240 250
EKVWSLTPSF RAEKSRTRRH LTEFWHLELE AAWMDLWDIM KVEEELVSYM
260 270 280 290 300
VQRTLELRKK EIEMFRDDLT TLKNTEPPFP RISYDEAIDI LQSKGVNVEW
310 320 330 340 350
GDDLGADEER VLTEEFDRPF FVYGYPKHIK AFYMKEDPND PRKVLASDML
360 370 380 390 400
APEGYGEIIG GSQREDDYDK LLNRILEEGM DPKDYEWYLD LRRYGSVPHS
410 420 430
GFGLGVERLV AWVLKLDHIR WAALFPRTPA RLYP
Length:434
Mass (Da):50,160
Last modified:August 1, 1998 - v1
Checksum:i458F1447BC0CD1DF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29313.1.
PIRiB71248.
RefSeqiWP_010884344.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA29313; BAA29313; BAA29313.
GeneIDi1444131.
KEGGipho:PH0241.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29313.1.
PIRiB71248.
RefSeqiWP_010884344.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1X54X-ray1.45A1-434[»]
1X55X-ray1.80A1-434[»]
1X56X-ray1.98A1-434[»]
ProteinModelPortaliO57980.
SMRiO57980. Positions 1-434.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH0241.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA29313; BAA29313; BAA29313.
GeneIDi1444131.
KEGGipho:PH0241.

Phylogenomic databases

eggNOGiarCOG00407. Archaea.
COG0017. LUCA.
HOGENOMiHOG000226034.
KOiK01893.
OMAiWGDDIGA.

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-232-MONOMER.
BRENDAi6.1.1.22. 5244.

Miscellaneous databases

EvolutionaryTraceiO57980.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00534. Asn_tRNA_synth. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004522. Asn-tRNA-ligase.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF16. PTHR22594:SF16. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00457. asnS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYN_PYRHO
AccessioniPrimary (citable) accession number: O57980
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: August 1, 1998
Last modified: September 7, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.