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Protein

Asparagine--tRNA ligase

Gene

asnS

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.1.1.22. 5244.

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine--tRNA ligaseUniRule annotation (EC:6.1.1.22UniRule annotation)
Alternative name(s):
Asparaginyl-tRNA synthetaseUniRule annotation
Short name:
AsnRSUniRule annotation
Gene namesi
Name:asnSUniRule annotation
Ordered Locus Names:PH0241
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001764871 – 434Asparagine--tRNA ligaseAdd BLAST434

Interactioni

Protein-protein interaction databases

STRINGi70601.PH0241.

Structurei

Secondary structure

1434
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 9Combined sources3
Helixi12 – 14Combined sources3
Beta strandi18 – 31Combined sources14
Beta strandi34 – 41Combined sources8
Beta strandi44 – 50Combined sources7
Helixi52 – 55Combined sources4
Helixi57 – 64Combined sources8
Beta strandi71 – 80Combined sources10
Helixi85 – 87Combined sources3
Beta strandi88 – 99Combined sources12
Helixi110 – 112Combined sources3
Helixi115 – 120Combined sources6
Helixi122 – 125Combined sources4
Helixi129 – 151Combined sources23
Beta strandi161 – 164Combined sources4
Helixi170 – 172Combined sources3
Beta strandi175 – 178Combined sources4
Beta strandi181 – 185Combined sources5
Helixi190 – 200Combined sources11
Beta strandi201 – 210Combined sources10
Beta strandi222 – 232Combined sources11
Helixi236 – 257Combined sources22
Helixi259 – 263Combined sources5
Helixi270 – 273Combined sources4
Beta strandi281 – 283Combined sources3
Helixi284 – 293Combined sources10
Helixi306 – 313Combined sources8
Beta strandi320 – 326Combined sources7
Helixi327 – 329Combined sources3
Beta strandi343 – 351Combined sources9
Turni352 – 355Combined sources4
Beta strandi356 – 364Combined sources9
Helixi368 – 377Combined sources10
Helixi382 – 385Combined sources4
Helixi386 – 390Combined sources5
Turni391 – 393Combined sources3
Beta strandi399 – 405Combined sources7
Helixi406 – 413Combined sources8
Helixi419 – 422Combined sources4
Beta strandi423 – 425Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X54X-ray1.45A1-434[»]
1X55X-ray1.80A1-434[»]
1X56X-ray1.98A1-434[»]
ProteinModelPortaliO57980.
SMRiO57980.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO57980.

Family & Domainsi

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00407. Archaea.
COG0017. LUCA.
HOGENOMiHOG000226034.
KOiK01893.
OMAiWGDDIGA.

Family and domain databases

HAMAPiMF_00534. Asn_tRNA_synth. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004522. Asn-tRNA-ligase.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF16. PTHR22594:SF16. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00457. asnS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O57980-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEKVYCQEV KPELDGKKVR LAGWVYTNMR VGKKIFLWIR DSTGIVQAVV
60 70 80 90 100
AKNVVGEETF EKAKKLGRES SVIVEGIVKA DERAPGGAEV HVEKLEVIQA
110 120 130 140 150
VSEFPIPENP EQASPELLLD YRHLHIRTPK ASAIMKVKET LIMAAREWLL
160 170 180 190 200
KDGWHEVFPP ILVTGAVEGG ATLFKLKYFD KYAYLSQSAQ LYLEAAIFGL
210 220 230 240 250
EKVWSLTPSF RAEKSRTRRH LTEFWHLELE AAWMDLWDIM KVEEELVSYM
260 270 280 290 300
VQRTLELRKK EIEMFRDDLT TLKNTEPPFP RISYDEAIDI LQSKGVNVEW
310 320 330 340 350
GDDLGADEER VLTEEFDRPF FVYGYPKHIK AFYMKEDPND PRKVLASDML
360 370 380 390 400
APEGYGEIIG GSQREDDYDK LLNRILEEGM DPKDYEWYLD LRRYGSVPHS
410 420 430
GFGLGVERLV AWVLKLDHIR WAALFPRTPA RLYP
Length:434
Mass (Da):50,160
Last modified:August 1, 1998 - v1
Checksum:i458F1447BC0CD1DF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29313.1.
PIRiB71248.
RefSeqiWP_010884344.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA29313; BAA29313; BAA29313.
GeneIDi1444131.
KEGGipho:PH0241.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29313.1.
PIRiB71248.
RefSeqiWP_010884344.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X54X-ray1.45A1-434[»]
1X55X-ray1.80A1-434[»]
1X56X-ray1.98A1-434[»]
ProteinModelPortaliO57980.
SMRiO57980.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH0241.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA29313; BAA29313; BAA29313.
GeneIDi1444131.
KEGGipho:PH0241.

Phylogenomic databases

eggNOGiarCOG00407. Archaea.
COG0017. LUCA.
HOGENOMiHOG000226034.
KOiK01893.
OMAiWGDDIGA.

Enzyme and pathway databases

BRENDAi6.1.1.22. 5244.

Miscellaneous databases

EvolutionaryTraceiO57980.

Family and domain databases

HAMAPiMF_00534. Asn_tRNA_synth. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004522. Asn-tRNA-ligase.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF16. PTHR22594:SF16. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00457. asnS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYN_PYRHO
AccessioniPrimary (citable) accession number: O57980
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.