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O57979

- PUR1_PYRHO

UniProt

O57979 - PUR1_PYRHO

Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity
    Binds 1 4Fe-4S cluster per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei10 – 101For GATase activityBy similarity
    Metal bindingi239 – 2391Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi286 – 2861MagnesiumBy similarity
    Metal bindingi346 – 3461MagnesiumBy similarity
    Metal bindingi347 – 3471MagnesiumBy similarity
    Metal bindingi383 – 3831Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi432 – 4321Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi435 – 4351Iron-sulfur (4Fe-4S)By similarity

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. amidophosphoribosyltransferase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
    2. glutamine metabolic process Source: UniProtKB-KW
    3. nucleoside metabolic process Source: InterPro
    4. purine nucleobase biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciPHOR70601:GJWR-231-MONOMER.
    UniPathwayiUPA00074; UER00124.

    Protein family/group databases

    MEROPSiC44.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Amidophosphoribosyltransferase (EC:2.4.2.14)
    Short name:
    ATase
    Alternative name(s):
    Glutamine phosphoribosylpyrophosphate amidotransferase
    Short name:
    GPATase
    Gene namesi
    Name:purF
    Ordered Locus Names:PH0240
    OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
    Taxonomic identifieri70601 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    ProteomesiUP000000752: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 99CuratedPRO_0000029279
    Chaini10 – 449440AmidophosphoribosyltransferasePRO_0000029280Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    IntActiO57979. 1 interaction.
    MINTiMINT-1501178.
    STRINGi70601.PH0240.

    Structurei

    3D structure databases

    ProteinModelPortaliO57979.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini10 – 224215Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated
    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    eggNOGiCOG0034.
    HOGENOMiHOG000033687.
    KOiK00764.
    OMAiIPVGDMN.

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    3.60.20.10. 1 hit.
    InterProiIPR005854. Amd_phspho_trans.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view]
    PfamiPF13537. GATase_7. 1 hit.
    PF00156. Pribosyltran. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
    SUPFAMiSSF53271. SSF53271. 1 hit.
    SSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR01134. purF. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O57979-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRGEIMKEKC GIFGAYSQDA TKKTYYGLMA LQHRGQEGAG ISVWDGDIRT    50
    VKGHGLVSEV FKGGSIRRLN GNPVIGHVRY STSGSLSEVQ PLEVECCGYK 100
    VSIAHNGTLT NFLPLRRFYE SRGFKFRSSI DTEVIAVSFL NHYSELKDEF 150
    EAMSRVFEEV KGAYSVLMLF NGKLIAVRDP VGFRPLSFGA GDGYYFSSED 200
    SALRMFCTNI RDVSPGEVIV VKDGEAESKI VGRSEHAYCV FEYIYFARPD 250
    SIINGISVYW ARYRMGVELA RESPAEGDVV IAVPDSGRTA ALGFAHESGI 300
    PYMEGLIKNR YIGRTFIMPS GREIKVRLKL SPVKEVIKGR RIVLVDDSIV 350
    RGTTMKNIVK MLRDAGAREV HVRIASPPIR YPCYMGIDIP TRHELIAAWK 400
    SIEEIKKEIG ADSLAYLSVE GLKRAIGTDK LCMACLTGNY PEWAFDFKV 449
    Length:449
    Mass (Da):50,052
    Last modified:August 1, 1998 - v1
    Checksum:iBA9E98A9FA1713A2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000001 Genomic DNA. Translation: BAA29312.1.
    PIRiA71248.
    RefSeqiNP_142236.1. NC_000961.1.
    WP_010884343.1. NC_000961.1.

    Genome annotation databases

    EnsemblBacteriaiBAA29312; BAA29312; BAA29312.
    GeneIDi1444130.
    KEGGipho:PH0240.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000001 Genomic DNA. Translation: BAA29312.1 .
    PIRi A71248.
    RefSeqi NP_142236.1. NC_000961.1.
    WP_010884343.1. NC_000961.1.

    3D structure databases

    ProteinModelPortali O57979.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O57979. 1 interaction.
    MINTi MINT-1501178.
    STRINGi 70601.PH0240.

    Protein family/group databases

    MEROPSi C44.001.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAA29312 ; BAA29312 ; BAA29312 .
    GeneIDi 1444130.
    KEGGi pho:PH0240.

    Phylogenomic databases

    eggNOGi COG0034.
    HOGENOMi HOG000033687.
    KOi K00764.
    OMAi IPVGDMN.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00124 .
    BioCyci PHOR70601:GJWR-231-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    3.60.20.10. 1 hit.
    InterProi IPR005854. Amd_phspho_trans.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view ]
    Pfami PF13537. GATase_7. 1 hit.
    PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000485. Amd_phspho_trans. 1 hit.
    SUPFAMi SSF53271. SSF53271. 1 hit.
    SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR01134. purF. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

    Entry informationi

    Entry nameiPUR1_PYRHO
    AccessioniPrimary (citable) accession number: O57979
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3