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Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.UniRule annotation

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei10 – 101NucleophileUniRule annotation
Metal bindingi239 – 2391Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi286 – 2861MagnesiumUniRule annotation
Metal bindingi346 – 3461MagnesiumUniRule annotation
Metal bindingi347 – 3471MagnesiumUniRule annotation
Metal bindingi383 – 3831Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi432 – 4321Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi435 – 4351Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. amidophosphoribosyltransferase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. glutamine metabolic process Source: UniProtKB-KW
  3. nucleoside metabolic process Source: InterPro
  4. purine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-231-MONOMER.
UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.A03.

Names & Taxonomyi

Protein namesi
Recommended name:
AmidophosphoribosyltransferaseUniRule annotation (EC:2.4.2.14UniRule annotation)
Short name:
ATaseUniRule annotation
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferaseUniRule annotation
Short name:
GPATaseUniRule annotation
Gene namesi
Name:purFUniRule annotation
Ordered Locus Names:PH0240
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000000752 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 99CuratedPRO_0000029279
Chaini10 – 449440AmidophosphoribosyltransferasePRO_0000029280Add
BLAST

Interactioni

Protein-protein interaction databases

IntActiO57979. 1 interaction.
MINTiMINT-1501178.
STRINGi70601.PH0240.

Structurei

3D structure databases

ProteinModelPortaliO57979.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 224215Glutamine amidotransferase type-2UniRule annotationAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation
Contains 1 glutamine amidotransferase type-2 domain.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0034.
HOGENOMiHOG000033687.
KOiK00764.
OMAiIAGVNVH.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF.
InterProiIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF13537. GATase_7. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O57979-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGEIMKEKC GIFGAYSQDA TKKTYYGLMA LQHRGQEGAG ISVWDGDIRT
60 70 80 90 100
VKGHGLVSEV FKGGSIRRLN GNPVIGHVRY STSGSLSEVQ PLEVECCGYK
110 120 130 140 150
VSIAHNGTLT NFLPLRRFYE SRGFKFRSSI DTEVIAVSFL NHYSELKDEF
160 170 180 190 200
EAMSRVFEEV KGAYSVLMLF NGKLIAVRDP VGFRPLSFGA GDGYYFSSED
210 220 230 240 250
SALRMFCTNI RDVSPGEVIV VKDGEAESKI VGRSEHAYCV FEYIYFARPD
260 270 280 290 300
SIINGISVYW ARYRMGVELA RESPAEGDVV IAVPDSGRTA ALGFAHESGI
310 320 330 340 350
PYMEGLIKNR YIGRTFIMPS GREIKVRLKL SPVKEVIKGR RIVLVDDSIV
360 370 380 390 400
RGTTMKNIVK MLRDAGAREV HVRIASPPIR YPCYMGIDIP TRHELIAAWK
410 420 430 440
SIEEIKKEIG ADSLAYLSVE GLKRAIGTDK LCMACLTGNY PEWAFDFKV
Length:449
Mass (Da):50,052
Last modified:July 31, 1998 - v1
Checksum:iBA9E98A9FA1713A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29312.1.
PIRiA71248.
RefSeqiNP_142236.1. NC_000961.1.
WP_010884343.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA29312; BAA29312; BAA29312.
GeneIDi1444130.
KEGGipho:PH0240.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29312.1.
PIRiA71248.
RefSeqiNP_142236.1. NC_000961.1.
WP_010884343.1. NC_000961.1.

3D structure databases

ProteinModelPortaliO57979.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO57979. 1 interaction.
MINTiMINT-1501178.
STRINGi70601.PH0240.

Protein family/group databases

MEROPSiC44.A03.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA29312; BAA29312; BAA29312.
GeneIDi1444130.
KEGGipho:PH0240.

Phylogenomic databases

eggNOGiCOG0034.
HOGENOMiHOG000033687.
KOiK00764.
OMAiIAGVNVH.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124.
BioCyciPHOR70601:GJWR-231-MONOMER.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF.
InterProiIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF13537. GATase_7. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Entry informationi

Entry nameiPUR1_PYRHO
AccessioniPrimary (citable) accession number: O57979
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2000
Last sequence update: July 31, 1998
Last modified: March 3, 2015
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.