Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O57979 (PUR1_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Amidophosphoribosyltransferase

Short name=ATase
EC=2.4.2.14
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Short name=GPATase
Gene names
Name:purF
Ordered Locus Names:PH0240
OrganismPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]
Taxonomic identifier70601 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length449 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Binds 1 4Fe-4S cluster per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.

Sequence similarities

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Contains 1 glutamine amidotransferase type-2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 99 Probable
PRO_0000029279
Chain10 – 449440Amidophosphoribosyltransferase
PRO_0000029280

Regions

Domain10 – 224215Glutamine amidotransferase type-2

Sites

Active site101For GATase activity By similarity
Metal binding2391Iron-sulfur (4Fe-4S) By similarity
Metal binding2861Magnesium By similarity
Metal binding3461Magnesium By similarity
Metal binding3471Magnesium By similarity
Metal binding3831Iron-sulfur (4Fe-4S) By similarity
Metal binding4321Iron-sulfur (4Fe-4S) By similarity
Metal binding4351Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
O57979 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: BA9E98A9FA1713A2

FASTA44950,052
        10         20         30         40         50         60 
MRGEIMKEKC GIFGAYSQDA TKKTYYGLMA LQHRGQEGAG ISVWDGDIRT VKGHGLVSEV 

        70         80         90        100        110        120 
FKGGSIRRLN GNPVIGHVRY STSGSLSEVQ PLEVECCGYK VSIAHNGTLT NFLPLRRFYE 

       130        140        150        160        170        180 
SRGFKFRSSI DTEVIAVSFL NHYSELKDEF EAMSRVFEEV KGAYSVLMLF NGKLIAVRDP 

       190        200        210        220        230        240 
VGFRPLSFGA GDGYYFSSED SALRMFCTNI RDVSPGEVIV VKDGEAESKI VGRSEHAYCV 

       250        260        270        280        290        300 
FEYIYFARPD SIINGISVYW ARYRMGVELA RESPAEGDVV IAVPDSGRTA ALGFAHESGI 

       310        320        330        340        350        360 
PYMEGLIKNR YIGRTFIMPS GREIKVRLKL SPVKEVIKGR RIVLVDDSIV RGTTMKNIVK 

       370        380        390        400        410        420 
MLRDAGAREV HVRIASPPIR YPCYMGIDIP TRHELIAAWK SIEEIKKEIG ADSLAYLSVE 

       430        440 
GLKRAIGTDK LCMACLTGNY PEWAFDFKV 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000001 Genomic DNA. Translation: BAA29312.1.
PIRA71248.
RefSeqNP_142236.1. NC_000961.1.

3D structure databases

ProteinModelPortalO57979.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActO57979. 1 interaction.
MINTMINT-1501178.
STRING70601.PH0240.

Protein family/group databases

MEROPSC44.001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA29312; BAA29312; BAA29312.
GeneID1444130.
KEGGpho:PH0240.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0034.
HOGENOMHOG000033687.
KOK00764.
OMACDACFTG.
ProtClustDBPRK08341.

Enzyme and pathway databases

BioCycPHOR70601:GJWR-231-MONOMER.
UniPathwayUPA00074; UER00124.

Family and domain databases

InterProIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR000836. PRibTrfase_dom.
[Graphical view]
PfamPF13537. GATase_7. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFPIRSF000485. Amd_phspho_trans. 1 hit.
TIGRFAMsTIGR01134. purF. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR1_PYRHO
AccessionPrimary (citable) accession number: O57979
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: October 16, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways