O57971 (ASGX_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative L-asparaginase
EC=3.5.1.1

Alternative name(s):
L-asparagine amidohydrolase

Cleaved into the following 2 chains:

1. Putative L-asparaginase subunit alpha
2. Putative L-asparaginase subunit beta

Gene names

Ordered Locus Names:

PH0232

Organism

Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]

Taxonomic identifier

70601 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-asparagine + H2O = L-aspartate + NH3.
Post-translational modification	Autocleaved. Generates the alpha and beta subunits. The N-terminal residue of the beta subunit is thought to be responsible for the nucleophile hydrolase activity Potential.
Sequence similarities	Belongs to the Ntn-hydrolase family.

Ontologies

Keywords
Molecular function	Hydrolase Protease
PTM	Autocatalytic cleavage
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	asparaginase activity Inferred from electronic annotation. Source: EC peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 174	174	Putative L-asparaginase subunit alpha		PRO_0000184581
	Chain	175 – 305	131	Putative L-asparaginase subunit beta		PRO_0000329018
Sites
	Active site	175	1	Nucleophile By similarity
	Site	174 – 175	2	Cleavage; by autolysis Potential

Sequences

	Sequence		Length	Mass (Da)
	O57971 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: C5391CAB69D48E03	FASTA	305	32,543
	10 20 30 40 50 60 MVAIIVHGGA GTIKKEERIP KVIEGVKEAV IVGWKELRKG SALDAVEEAI KVLEDNPIFN 70 80 90 100 110 120 AGTGSVLTID GKVEMDAAIM RGKTLEAGAV AGIWGVKNPI SVARKVMEKT DHVLLVGEGA 130 140 150 160 170 180 VKFARLMGFP EYNPITEERI EQWKELKEKL MKGEIKYWKK LGELIKEYPE VLRSTVGAVA 190 200 210 220 230 240 FDGEEIVAGT STGGVFLKMF GRVGDTPIIG AGTYANEVAG ASCTGLGEVA IRLALAKTAT 250 260 270 280 290 300 DFVRLGMDAQ AASNAAISLA TKYFGKDTMG IIMVDAAGNV GFAKNTKHMS YAYMKDGMEE PEAGV

References

[1]

"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3." Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H. DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000001 Genomic DNA. Translation: BAA29304.1.
PIR	A71247.
RefSeq	NP_142228.1. NC_000961.1.
3D structure databases
ProteinModelPortal	O57971.
ModBase	Search...
Protein family/group databases
MEROPS	T02.002.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000001542; EBPYRP00000001542; EBPYRG00000001542.
GeneID	1444122.
GenomeReviews	Gene locus PH0232 in contig BA000001_GR.
KEGG	pho:PH0232.
NMPDR	fig|70601.1.peg.222.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000022719.
HOGENOM	HBG735787.
OMA	WAVKWTS.
PhylomeDB	O57971.
ProtClustDB	CLSK253204.
Enzyme and pathway databases
BioCyc	PHOR70601:PH0232-MONOMER.
Family and domain databases
InterPro	IPR000246. Peptidase_T2. [Graphical view]
KO	K13051.
PANTHER	PTHR10188. Peptidase_T2. 1 hit.
Pfam	PF01112. Asparaginase_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASGX_PYRHO

Accession

Primary (citable) accession number: O57971

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	December 14, 2011
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program