O57932 (AMYA_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-amylase
EC=3.2.1.1

Gene names

Name:	amyA
Ordered Locus Names:	PH0193

Organism

Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]

Taxonomic identifier

70601 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	633 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.
Sequence similarities	Belongs to the glycosyl hydrolase 57 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-amylase activity Inferred from electronic annotation. Source: EC alpha-mannosidase activity Inferred from electronic annotation. Source: InterPro carbohydrate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 633	633	Alpha-amylase		PRO_0000184574
Sites
	Active site	123	1	Nucleophile By similarity
	Active site	214	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	O57932 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 9DCBCBB2FE191501	FASTA	633	75,018
	10 20 30 40 50 60 MPRINFIFGV HNHQPLGNFE WIIKRAYEKA YRPFLETLEE YPNMKVAVHI SGVLVEWLER 70 80 90 100 110 120 NRPEYIDLLK SLIKKGQVEL VVAGFYEPIL VAIPEEDRVE QIKLSKGWAR KMGYEARGLW 130 140 150 160 170 180 LTERVWEPEL VKTLREAGIE YVILDDYHFM SAGLSKEELF WPYYTENGGE AIVVFPIDEK 190 200 210 220 230 240 LRYLIPFRPV NETLEYLHSL ADEDESKVAV FHDDGEKFGA WPGTHELVYE RGWLKEFFDR 250 260 270 280 290 300 ISSDDKINLM LYSEYLSKFR PKGLVYLPIA SYFEMSEWSL PARQAKLFFE FIKKLKELNL 310 320 330 340 350 360 FEKYRIFVRG GIWKNFLYKY PEGNYMHKRM LMLSKLLRNN PTARIFVLRA QCNDAYWHGV 370 380 390 400 410 420 FGGIYLPHIR RAVWRNLIKA HSYLEPENRV FDLDFDGGEE IMLENENFIL VVKPHYGGAI 430 440 450 460 470 480 FEMSSKKKYV NYLDVVARRW EHYHSLKDIP EGMKRELSYD KWPRGMLQDH FLLPTEVLDN 490 500 510 520 530 540 YMLSKYRELG DFLMSSYHYQ IEDKLRLWRS GKVKGISVEV EKVLRLNKDG FTTEYRIVSK 550 560 570 580 590 600 EELGLMFGVE INLAVQGTVE YPAEFMSKEI EVKDIFGKVK IESEKEAKIW KFPIKTLSQS 610 620 630 ESGWDFVQQG VSYTFLYPIE KMLNIKLKFK ESM

References

[1]

"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3." Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H. DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000001 Genomic DNA. Translation: BAA29262.1.
PIR	G71241.
RefSeq	NP_142192.1. NC_000961.1.
3D structure databases
ProteinModelPortal	O57932.
SMR	O57932. Positions 3-631.
ModBase	Search...
Protein family/group databases
CAZy	GH57. Glycoside Hydrolase Family 57.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000001706; EBPYRP00000001706; EBPYRG00000001706.
GeneID	1444084.
GenomeReviews	Gene locus PH0193 in contig BA000001_GR.
KEGG	pho:PH0193.
NMPDR	fig|70601.1.peg.184.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000023113.
HOGENOM	HBG460637.
OMA	LTERVWE.
ProtClustDB	CLSK2459743.
Enzyme and pathway databases
BioCyc	PHOR70601:PH0193-MONOMER.
Family and domain databases
InterPro	IPR015179. A-amylase/a-glucTrfase_C. IPR015178. A-amylase/a-glucTrfase_central. IPR011013. Glyco_hydro-type_carb-bd. IPR011330. Glyco_hydro/deAcase_b/a-brl. IPR000602. Glyco_hydro_38_core. IPR004300. Glyco_hydro_57_N. [Graphical view]
Gene3D	G3DSA:3.20.110.10. Glyco_hydro_38_core. 1 hit.
KO	K07405.
Pfam	PF09094. DUF1925. 1 hit. PF09095. DUF1926. 1 hit. PF03065. Glyco_hydro_57. 1 hit. [Graphical view]
ProDom	PD014405. DUF1925. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF74650. Gal_mut_like. 1 hit. SSF88713. Glyco_hydro/deAcase_b/a-brl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AMYA_PYRHO

Accession

Primary (citable) accession number: O57932

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	December 14, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program