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O57921 (NADE_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5
Gene names
Name:nadE
Ordered Locus Names:PH0182
OrganismPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]
Taxonomic identifier70601 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length257 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193

Pathway

Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193

Sequence similarities

Belongs to the NAD synthetase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 257257Probable NH(3)-dependent NAD(+) synthetase HAMAP MF_00193
PRO_0000152232

Regions

Nucleotide binding28 – 358ATP By similarity

Sites

Active site301 By similarity

Secondary structure

1 .......................................... 257
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O57921 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 86A9CF2CC6F387FC

FASTA25728,862
        10         20         30         40         50         60 
MRILDYDKVI ERILEFIREK GNNGVVIGIS GGVDSATVAY LATKALGKEK VLGLIMPYFE 

        70         80         90        100        110        120 
NKDVEDAKLV AEKLGIGYKV INIKPIVDSF VENLELNLDR KGLGNIMSRT RMIMLYAHAN 

       130        140        150        160        170        180 
SLGRIVLGTS NRSEFLTGYF TKWGDGASDY APIINLYKTE VWEIAKRIGV PERIVKKKPS 

       190        200        210        220        230        240 
AGLWEGQTDE DELGISYNLL DEILWRMIDL KIGKEEIAKD LGIPLSLVER VEELIKKSEH 

       250 
KRRLPIGPSF EDLIVGP

« Hide

References

[1]"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3."
Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. expand/collapse author list , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.
DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000001 Genomic DNA. Translation: BAA29251.1.
PIRD71240.
RefSeqNP_142181.1. NC_000961.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2E18X-ray2.10A/B1-257[»]
ProteinModelPortalO57921.
SMRO57921. Positions 1-256.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBPYRT00000000602; EBPYRP00000000602; EBPYRG00000000602.
GeneID1444073.
GenomeReviewsGene locus PH0182 in contig BA000001_GR.
KEGGpho:PH0182.
NMPDRfig|70601.1.peg.173.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000022961.
HOGENOMHBG351567.
OMADGAVDCH.
PhylomeDBO57921.
ProtClustDBPRK13980.

Enzyme and pathway databases

BioCycPHOR70601:PH0182-MONOMER.

Family and domain databases

HAMAPMF_00193. NadE.
[Tree]
InterProIPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR018126. SASP_alpha/beta-type_CS.
[Graphical view]
Gene3DG3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KOK01916.
PfamPF02540. NAD_synthase. 1 hit.
[Graphical view]
TIGRFAMsTIGR00552. NadE. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNADE_PYRHO
AccessionPrimary (citable) accession number: O57921
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 1, 1998
Last modified: December 14, 2011
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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