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O57865 (MTAP_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
S-methyl-5'-thioadenosine phosphorylase

EC=2.4.2.28
Alternative name(s):
5'-methylthioadenosine phosphorylase
Short name=MTA phosphorylase
Short name=MTAP
Gene names
Name:mtnP
Ordered Locus Names:PH0125
OrganismPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]
Taxonomic identifier70601 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length257 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates By similarity. HAMAP-Rule MF_01963

Catalytic activity

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate. HAMAP-Rule MF_01963

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route): step 1/1. HAMAP-Rule MF_01963

Subunit structure

Homohexamer. Dimer of a homotrimer By similarity. HAMAP-Rule MF_01963

Sequence similarities

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.

Sequence caution

The sequence BAA29194.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 257257S-methyl-5'-thioadenosine phosphorylase HAMAP-Rule MF_01963
PRO_0000184561

Regions

Region50 – 512Phosphate binding By similarity
Region204 – 2063Substrate binding By similarity

Sites

Binding site101Phosphate By similarity
Binding site1801Substrate; via amide nitrogen By similarity
Binding site1811Phosphate By similarity
Site1631Important for substrate specificity By similarity
Site2151Important for substrate specificity By similarity

Sequences

Sequence LengthMass (Da)Tools
O57865 [UniParc].

Last modified February 21, 2001. Version 2.
Checksum: 35A162D939A89E90

FASTA25729,175
        10         20         30         40         50         60 
MPKIGIIGGS GVYGVFEPKE VVKVHTPYGR PSAPIEIGEI EGVEVAFIPR HGKYHEFPPH 

        70         80         90        100        110        120 
QVPYRANIWA LHELGVERVI AINAVGSLKE EYKPGDIVII DQFIDFTKKR EYTFYNGPKV 

       130        140        150        160        170        180 
AHVSMADPFC PELRKIFIET AKELNLPVHE RGTYVCIEGP RFSTRAESRM FRQFADVIGM 

       190        200        210        220        230        240 
TLVPEVNLAR ELGMCYVNIS TVTDYDVWAE KPVDAQEVLR VMKENEEKVQ KLLKRAIPKI 

       250 
PEERKCGCAD VLKTMFV 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000001 Genomic DNA. Translation: BAA29194.1. Different initiation.
PIRC71233.
RefSeqNP_142133.1. NC_000961.1.

3D structure databases

ProteinModelPortalO57865.
SMRO57865. Positions 4-243.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-1501612.
STRING70601.PH0125.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA29194; BAA29194; BAA29194.
GeneID1444021.
KEGGpho:PH0125.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0005.
HOGENOMHOG000228987.
KOK00772.
OMAGCADVLK.
ProtClustDBPRK08564.

Enzyme and pathway databases

BioCycPHOR70601:GJWR-122-MONOMER.
UniPathwayUPA00904; UER00873.

Family and domain databases

Gene3D3.40.50.1580. 1 hit.
HAMAPMF_01963. MTAP.
InterProIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERPTHR11904. PTHR11904. 1 hit.
PfamPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMSSF53167. SSF53167. 1 hit.
TIGRFAMsTIGR01694. MTAP. 1 hit.
PROSITEPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTAP_PYRHO
AccessionPrimary (citable) accession number: O57865
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 21, 2001
Last modified: April 16, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways