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Protein

S-methyl-5'-thioadenosine phosphorylase

Gene

mtnP

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.UniRule annotation

Catalytic activityi

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. S-methyl-5'-thioadenosine phosphorylase (mtnP)
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route), the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10PhosphateUniRule annotation1
Sitei163Important for substrate specificityUniRule annotation1
Binding sitei180Substrate; via amide nitrogenUniRule annotation1
Binding sitei181PhosphateUniRule annotation1
Sitei215Important for substrate specificityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processPurine salvage

Enzyme and pathway databases

BioCyciPHOR70601:G1G39-118-MONOMER
UniPathwayiUPA00904; UER00873

Names & Taxonomyi

Protein namesi
Recommended name:
S-methyl-5'-thioadenosine phosphorylaseUniRule annotation (EC:2.4.2.28UniRule annotation)
Alternative name(s):
5'-methylthioadenosine phosphorylaseUniRule annotation
Short name:
MTA phosphorylaseUniRule annotation
Short name:
MTAPUniRule annotation
Gene namesi
Name:mtnPUniRule annotation
Ordered Locus Names:PH0125
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001845611 – 257S-methyl-5'-thioadenosine phosphorylaseAdd BLAST257

Interactioni

Subunit structurei

Homohexamer. Dimer of a homotrimer.UniRule annotation

Protein-protein interaction databases

MINTiO57865
STRINGi70601.PH0125

Structurei

3D structure databases

ProteinModelPortaliO57865
SMRiO57865
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni50 – 51Phosphate bindingUniRule annotation2
Regioni204 – 206Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01327 Archaea
COG0005 LUCA
HOGENOMiHOG000228987
KOiK00772
OMAiNLYRSWG
OrthoDBiPOG093Z08FG

Family and domain databases

HAMAPiMF_01963 MTAP, 1 hit
InterProiView protein in InterPro
IPR010044 MTAP
IPR000845 Nucleoside_phosphorylase_d
IPR035994 Nucleoside_phosphorylase_sf
IPR018099 Purine_phosphorylase-2_CS
PANTHERiPTHR42679 PTHR42679, 1 hit
PfamiView protein in Pfam
PF01048 PNP_UDP_1, 1 hit
SUPFAMiSSF53167 SSF53167, 1 hit
TIGRFAMsiTIGR01694 MTAP, 1 hit
PROSITEiView protein in PROSITE
PS01240 PNP_MTAP_2, 1 hit

Sequencei

Sequence statusi: Complete.

O57865-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKIGIIGGS GVYGVFEPKE VVKVHTPYGR PSAPIEIGEI EGVEVAFIPR
60 70 80 90 100
HGKYHEFPPH QVPYRANIWA LHELGVERVI AINAVGSLKE EYKPGDIVII
110 120 130 140 150
DQFIDFTKKR EYTFYNGPKV AHVSMADPFC PELRKIFIET AKELNLPVHE
160 170 180 190 200
RGTYVCIEGP RFSTRAESRM FRQFADVIGM TLVPEVNLAR ELGMCYVNIS
210 220 230 240 250
TVTDYDVWAE KPVDAQEVLR VMKENEEKVQ KLLKRAIPKI PEERKCGCAD

VLKTMFV
Length:257
Mass (Da):29,175
Last modified:February 21, 2001 - v2
Checksum:i35A162D939A89E90
GO

Sequence cautioni

The sequence BAA29194 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA Translation: BAA29194.1 Different initiation.
PIRiC71233
RefSeqiWP_048053039.1, NC_000961.1

Genome annotation databases

EnsemblBacteriaiBAA29194; BAA29194; BAA29194
GeneIDi1444021
KEGGipho:PH0125

Similar proteinsi

Entry informationi

Entry nameiMTAP_PYRHO
AccessioniPrimary (citable) accession number: O57865
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 21, 2001
Last modified: April 25, 2018
This is version 97 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health