Reviewed, UniProtKB/Swiss-Prot O57861 (DP2L_PYRHO)

Names and origin

Protein names

Recommended name:
    DNA polymerase II large subunit
      Short name=Pol II
    EC=2.7.7.7
Cleaved into the following chain:
    1- Recommended name:
            Pho polC intein
        Alternative name(s):
            Pho pol II intein

Gene names

Name:	polC
Ordered Locus Names:	PH0121

Organism

Pyrococcus horikoshii [Complete proteome] [HAMAP]

Taxonomic identifier

53953 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	1431 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_00324 Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP MF_00324
Subunit structure	Heterodimer of a large subunit and a small subunit By similarity.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.
Sequence similarities	Belongs to the archaeal DNA polymerase II family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	DNA catabolic process, exonucleolytic Inferred from electronic annotation. Source: HAMAP DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 951	951	DNA polymerase II large subunit, 1st part Potential		PRO_0000007307
	Chain	952 – 1117	166	Pho polC intein Potential		PRO_0000007308
	Chain	1118 – 1431	314	DNA polymerase II large subunit, 2nd part Potential		PRO_0000007309

Sequences

	Sequence		Length	Mass (Da)
	O57861-1 [UniParc]. Last modified April 27, 2001. Version 2. Checksum: 59B73A48868B6298	FASTA	1,431	162,928
	10 20 30 40 50 60 MELPKEMEEY FSMLQREIDK AYEIAKKARA QGKDPSLDVE IPQASDMAGR VESLVGPPGV 70 80 90 100 110 120 AERIRELVKE YGKEIAALKI VDEIIDGKFG DLGSKEKYAE QAVRTALAIL TEGVVSAPIE 130 140 150 160 170 180 GIASVKIKRN TWSDNSEYLA LYYAGPIRSS GGTAQALSVL VGDYVRRKLG LDRFKPSEKH 190 200 210 220 230 240 IERMVEEVDL YHRTVSRLQY HPSPEEVRLA MRNIPIEITG EATDEVEVSH RDIPGVETNQ 250 260 270 280 290 300 LRGGAILVLA EGVLQKAKKL VKYIDKMGIE GWEWLKEFVE AKEKGEEIEE EGSAESTVEE 310 320 330 340 350 360 TKVEVDMGFY YSLYQKFKSE IAPNDKYAKE IIGGRPLFSD PSRNGGFRLR YGRSRVSGFA 370 380 390 400 410 420 TWGINPATMI LVDEFLAIGT QLKTERPGKG AVVTPVTTIE GPIVKLKDGS VVKVDDYKLA 430 440 450 460 470 480 LKIRDEVEEI LYLGDAVIAF GDFVENNQTL LPANYCEEWW ILEFTKALNE IYEVELKPFE 490 500 510 520 530 540 VNSSEDLEEA ADYLEVDIEF LKELLKDPLR TKPPVELAIH FSEILGIPLH PYYTLYWNSV 550 560 570 580 590 600 KPEQVEKLWR VLKEHAHIDW DNFRGIKFAR RIVIPLEKLR DSKRALELLG LPHKVEGKNV 610 620 630 640 650 660 IVDYPWAAAL LTPLGNLEWE FRAKPLHTTI DIINENNEIK LRDRGISWIG ARMGRPEKAK 670 680 690 700 710 720 ERKMKPPVQV LFPIGLAGGS SRDIKKAAEE GKVAEVEIAL FKCPKCGHVG PEHICPNCGT 730 740 750 760 770 780 RKELIWVCPR CNAEYPESQA SGYNYTCPKC NVKLKPYAKR KIKPSELLKR AMDNVKVYGI 790 800 810 820 830 840 DKLKGVMGMT SGWKMPEPLE KGLLRAKNDV YVFKDGTIRF DATDAPITHF RPREIGVSVE 850 860 870 880 890 900 KLRELGYTHD FEGNPLVSED QIVELKPQDI ILSKEAGKYL LKVAKFVDDL LEKFYGLPRF 910 920 930 940 950 960 YNAEKMEDLI GHLVIGLAPH TSAGIVGRII GFVDALVGYA HPYFHAAKRR NCFPGDTRIL 970 980 990 1000 1010 1020 VQINGTPQRV TLKELYELFD EEHYESMVYV RKKPKVDIKV YSFNPEEGKV VLTDIEEVIK 1030 1040 1050 1060 1070 1080 APATDHLIRF ELELGSSFET TVDHPVLVYE NGKFVEKRAF EVREGNIIII IDESTLEPLK 1090 1100 1110 1120 1130 1140 VAVKKIEFIE PPEDFVFSLN AKKYHTVIIN ENIVTHQCDG DEDAVMLLLD ALLNFSRYYL 1150 1160 1170 1180 1190 1200 PEKRGGKMDA PLVITTRLDP REVDSEVHNM DIVRYYPLEF YEATYELKSP KELVGVIERV 1210 1220 1230 1240 1250 1260 EDRLGKPEMY YGLKFTHDTD DIALGPKMSL YKQLGDMEEK VKRQLDVARR IRAVDEHKVA 1270 1280 1290 1300 1310 1320 ETILNSHLIP DLRGNLRSFT RQEFRCVKCN TKFRRPPLDG KCPICGGKIV LTVSKGAIEK 1330 1340 1350 1360 1370 1380 YLGTAKMLVT EYKVKNYTRQ RICLTERDID SLFETVFPET QLTLLVNPND ICQRIIMERT 1390 1400 1410 1420 1430 GGSKKSGLLE NFANGYNKGK KEEMPKKQRK KEQEKSKKRK VISLDDFFSR K

References

[1]

"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3." Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H. DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OT3.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000001 Genomic DNA. Translation: BAA29190.1. Different initiation.
PIR	G71232.
RefSeq	NP_142130.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1444018.
GenomeReviews	Gene locus PH0121 in contig BA000001_GR.
KEGG	pho:PH0121.
NMPDR	fig|70601.1.peg.118.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O57861.
OMA	NCDGDED
Enzyme and pathway databases
BioCyc	PHOR70601:PH0121-MON.
BRENDA	2.7.7.7. 74679.
Family and domain databases
HAMAP	MF_00324. [Tree]
InterPro	IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR006141. Intein_splicing_site. IPR004475. PolC_DP2. IPR016033. PolC_DP2_N. [Graphical view]
Pfam	PF03833. PolC_DP2. 1 hit. [Graphical view]
SMART	SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
TIGRFAMs	TIGR01445. intein_Nterm. 1 hit. TIGR00354. polC. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DP2L_PYRHO

Accession

Primary (citable) accession number: O57861

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	April 27, 2001
Last modified:	November 24, 2009
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)