Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Replication factor C small subunit

Gene

rfcS

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replication factor C small subunit
Short name:
RFC small subunit
Alternative name(s):
Clamp loader small subunit
Cleaved into the following chain:
Gene namesi
Name:rfcS
Ordered Locus Names:PH0112
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000303791 – 61Replication factor C small subunit, 1st partSequence analysisAdd BLAST61
ChainiPRO_000003038062 – 586Pho RFC inteinSequence analysisAdd BLAST525
ChainiPRO_0000030381587 – 855Replication factor C small subunit, 2nd partSequence analysisAdd BLAST269

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Subunit structurei

Heteromultimer composed of small subunits (RfcS) and large subunits (RfcL).By similarity

Protein-protein interaction databases

STRINGi70601.PH0112.

Structurei

3D structure databases

ProteinModelPortaliO57852.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini185 – 308DOD-type homing endonucleasePROSITE-ProRule annotationAdd BLAST124

Sequence similaritiesi

Contains 1 DOD-type homing endonuclease domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiarCOG00469. Archaea.
arCOG03154. Archaea.
COG0470. LUCA.
COG1372. LUCA.
HOGENOMiHOG000154101.
KOiK04801.
OMAiRFMELTE.

Family and domain databases

Gene3Di1.10.260.40. 1 hit.
2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR001387. Cro/C1-type_HTH.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR030934. Intein_C.
IPR004042. Intein_endonuc.
IPR006141. Intein_N.
IPR004860. LAGLIDADG_2.
IPR010982. Lambda_DNA-bd_dom.
IPR027417. P-loop_NTPase.
IPR013748. Rep_factorC_C.
IPR008824. RuvB_N.
[Graphical view]
PfamiPF14528. LAGLIDADG_3. 1 hit.
PF08542. Rep_fac_C. 1 hit.
PF05496. RuvB_N. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
SM00530. HTH_XRE. 1 hit.
[Graphical view]
SUPFAMiSSF48019. SSF48019. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 2 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O57852-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHNMEEVREV KVLEKPWVEK YRPQRLDEIV GQEHIVKRLK HYVKTGSMPH
60 70 80 90 100
LLFAGPPGVG KCLTGDTKVI ANGQLFELRE LVEKISGGKF GPTPVKGLKV
110 120 130 140 150
IGIDEDGKLR EFEVQYVYKD KTERLIRIRT RLGRELKVTP YHPLLVNRRN
160 170 180 190 200
GEIKWVKAEE LKPGDKLAVP RFLPIVTGED PLAEWLGYFL GGGYADSKEN
210 220 230 240 250
LIMFTNEDPL LRQRFMELTE KLFSDARIRE ITHENGTSKV YVNSKKALKL
260 270 280 290 300
VNSLGNAHIP KECWRGIRSF LRAYFDCNGG VKGNAIVLAT ASKEMSQEIA
310 320 330 340 350
YALAGFGIIS RIQEYRVIIS GSDNVKKFLN EIGFINRNKL EKALKLVKKD
360 370 380 390 400
DPGHDGLEIN YELISYVKDR LRLSFFNDKR SWSYREAKEI SWELMKEIYY
410 420 430 440 450
RLDELEKLKE SLSRGILIDW NEVAKRIEEV AEETGIRADE LLEYIEGKRK
460 470 480 490 500
LSFKDYIKIA KVLGIDVEHT IEAMRVFARK YSSYAEIGRR LGTWNSSVKT
510 520 530 540 550
ILESNAVNVE ILERIRKIEL ELIEEILSDE KLKEGIAYLI FLSQNELYWD
560 570 580 590 600
EITKVEELRG EFIIYDLHVP GYHNFIAGNM PTVVHNTTAA LALSRELFGE
610 620 630 640 650
NWRHNFLELN ASDERGINVI REKVKEFART KPIGGASFKI IFLDEADALT
660 670 680 690 700
QDAQQALRRT MEMFSSNVRF ILSCNYSSKI IEPIQSRCAI FRFRPLRDED
710 720 730 740 750
IAKRLRYIAE NEGLELTEEG LQAILYIAEG DMRRAINILQ AAAALDKKIT
760 770 780 790 800
DENVFMVASR ARPEDIREMM LLALKGNFLK AREKLREILL KQGLSGEDVL
810 820 830 840 850
IQMHKEVFNL PIDEPTKVYL ADKIGEYNFR LVEGANEMIQ LEALLAQFTL

VGKKK
Length:855
Mass (Da):98,422
Last modified:August 1, 1998 - v1
Checksum:iFAD533FFD08FCF27
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29181.1.
PIRiF71231.

Genome annotation databases

EnsemblBacteriaiBAA29181; BAA29181; BAA29181.
KEGGipho:PH0112.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29181.1.
PIRiF71231.

3D structure databases

ProteinModelPortaliO57852.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH0112.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA29181; BAA29181; BAA29181.
KEGGipho:PH0112.

Phylogenomic databases

eggNOGiarCOG00469. Archaea.
arCOG03154. Archaea.
COG0470. LUCA.
COG1372. LUCA.
HOGENOMiHOG000154101.
KOiK04801.
OMAiRFMELTE.

Family and domain databases

Gene3Di1.10.260.40. 1 hit.
2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR001387. Cro/C1-type_HTH.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR030934. Intein_C.
IPR004042. Intein_endonuc.
IPR006141. Intein_N.
IPR004860. LAGLIDADG_2.
IPR010982. Lambda_DNA-bd_dom.
IPR027417. P-loop_NTPase.
IPR013748. Rep_factorC_C.
IPR008824. RuvB_N.
[Graphical view]
PfamiPF14528. LAGLIDADG_3. 1 hit.
PF08542. Rep_fac_C. 1 hit.
PF05496. RuvB_N. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
SM00530. HTH_XRE. 1 hit.
[Graphical view]
SUPFAMiSSF48019. SSF48019. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 2 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRFCS_PYRHO
AccessioniPrimary (citable) accession number: O57852
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: August 1, 1998
Last modified: November 30, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The intein interrupts the potential ATP-binding site.

Keywords - Technical termi

Complete proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.