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Protein

Replication factor C small subunit

Gene

rfcS

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA.By similarity

Miscellaneous

The intein interrupts the potential ATP-binding site.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processDNA replication
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replication factor C small subunit
Short name:
RFC small subunit
Alternative name(s):
Clamp loader small subunit
Cleaved into the following chain:
Gene namesi
Name:rfcS
Ordered Locus Names:PH0112
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000303791 – 61Replication factor C small subunit, 1st partSequence analysisAdd BLAST61
ChainiPRO_000003038062 – 586Pho RFC inteinSequence analysisAdd BLAST525
ChainiPRO_0000030381587 – 855Replication factor C small subunit, 2nd partSequence analysisAdd BLAST269

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Proteomic databases

PRIDEiO57852

Interactioni

Subunit structurei

Heteromultimer composed of small subunits (RfcS) and large subunits (RfcL).By similarity

Protein-protein interaction databases

STRINGi70601.PH0112

Structurei

3D structure databases

ProteinModelPortaliO57852
SMRiO57852
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini185 – 308DOD-type homing endonucleasePROSITE-ProRule annotationAdd BLAST124

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG00469 Archaea
arCOG03154 Archaea
COG0470 LUCA
COG1372 LUCA
HOGENOMiHOG000154101
KOiK04801
OMAiDGHADSK
OrthoDBiPOG093Z05CK

Family and domain databases

CDDicd00093 HTH_XRE, 1 hit
Gene3Di3.10.28.10, 1 hit
InterProiView protein in InterPro
IPR001387 Cro/C1-type_HTH
IPR008921 DNA_pol3_clamp-load_cplx_C
IPR003586 Hint_dom_C
IPR003587 Hint_dom_N
IPR036844 Hint_dom_sf
IPR027434 Homing_endonucl
IPR006142 INTEIN
IPR030934 Intein_C
IPR004042 Intein_endonuc
IPR006141 Intein_N
IPR004860 LAGLIDADG_2
IPR027417 P-loop_NTPase
IPR013748 Rep_factorC_C
IPR008824 RuvB_N
PfamiView protein in Pfam
PF14528 LAGLIDADG_3, 1 hit
PF08542 Rep_fac_C, 1 hit
PF05496 RuvB_N, 1 hit
PRINTSiPR00379 INTEIN
SMARTiView protein in SMART
SM00305 HintC, 1 hit
SM00306 HintN, 1 hit
SM00530 HTH_XRE, 1 hit
SUPFAMiSSF48019 SSF48019, 1 hit
SSF51294 SSF51294, 2 hits
SSF52540 SSF52540, 2 hits
SSF55608 SSF55608, 1 hit
TIGRFAMsiTIGR01443 intein_Cterm, 1 hit
TIGR01445 intein_Nterm, 1 hit
PROSITEiView protein in PROSITE
PS50818 INTEIN_C_TER, 1 hit
PS50819 INTEIN_ENDONUCLEASE, 1 hit
PS50817 INTEIN_N_TER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O57852-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHNMEEVREV KVLEKPWVEK YRPQRLDEIV GQEHIVKRLK HYVKTGSMPH
60 70 80 90 100
LLFAGPPGVG KCLTGDTKVI ANGQLFELRE LVEKISGGKF GPTPVKGLKV
110 120 130 140 150
IGIDEDGKLR EFEVQYVYKD KTERLIRIRT RLGRELKVTP YHPLLVNRRN
160 170 180 190 200
GEIKWVKAEE LKPGDKLAVP RFLPIVTGED PLAEWLGYFL GGGYADSKEN
210 220 230 240 250
LIMFTNEDPL LRQRFMELTE KLFSDARIRE ITHENGTSKV YVNSKKALKL
260 270 280 290 300
VNSLGNAHIP KECWRGIRSF LRAYFDCNGG VKGNAIVLAT ASKEMSQEIA
310 320 330 340 350
YALAGFGIIS RIQEYRVIIS GSDNVKKFLN EIGFINRNKL EKALKLVKKD
360 370 380 390 400
DPGHDGLEIN YELISYVKDR LRLSFFNDKR SWSYREAKEI SWELMKEIYY
410 420 430 440 450
RLDELEKLKE SLSRGILIDW NEVAKRIEEV AEETGIRADE LLEYIEGKRK
460 470 480 490 500
LSFKDYIKIA KVLGIDVEHT IEAMRVFARK YSSYAEIGRR LGTWNSSVKT
510 520 530 540 550
ILESNAVNVE ILERIRKIEL ELIEEILSDE KLKEGIAYLI FLSQNELYWD
560 570 580 590 600
EITKVEELRG EFIIYDLHVP GYHNFIAGNM PTVVHNTTAA LALSRELFGE
610 620 630 640 650
NWRHNFLELN ASDERGINVI REKVKEFART KPIGGASFKI IFLDEADALT
660 670 680 690 700
QDAQQALRRT MEMFSSNVRF ILSCNYSSKI IEPIQSRCAI FRFRPLRDED
710 720 730 740 750
IAKRLRYIAE NEGLELTEEG LQAILYIAEG DMRRAINILQ AAAALDKKIT
760 770 780 790 800
DENVFMVASR ARPEDIREMM LLALKGNFLK AREKLREILL KQGLSGEDVL
810 820 830 840 850
IQMHKEVFNL PIDEPTKVYL ADKIGEYNFR LVEGANEMIQ LEALLAQFTL

VGKKK
Length:855
Mass (Da):98,422
Last modified:August 1, 1998 - v1
Checksum:iFAD533FFD08FCF27
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA Translation: BAA29181.1
PIRiF71231

Genome annotation databases

EnsemblBacteriaiBAA29181; BAA29181; BAA29181
KEGGipho:PH0112

Similar proteinsi

Entry informationi

Entry nameiRFCS_PYRHO
AccessioniPrimary (citable) accession number: O57852
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: August 1, 1998
Last modified: May 23, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

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