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Protein

Alanyl-tRNA editing protein AlaX-M

Gene

alaXM

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Functions in trans to edit the amino acid moiety from mischarged charged Gly-tRNA(Ala) and Ser-tRNA(Ala).1 Publication

Cofactori

Zn2+1 PublicationNote: Binds 1 zinc ion per subunit.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi99 – 991Zinc
Metal bindingi103 – 1031Zinc
Metal bindingi182 – 1821Zinc

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-109-MONOMER.
BRENDAi3.1.1.29. 5244.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanyl-tRNA editing protein AlaX-M
Short name:
AlaX-M
Alternative name(s):
Alanyl-tRNA deacylase AlaX-M
Gene namesi
Name:alaXM
Ordered Locus Names:PH0108
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 216216Alanyl-tRNA editing protein AlaX-MPRO_0000391648Add
BLAST

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi70601.PH0108.

Structurei

Secondary structure

1
216
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi17 – 204Combined sources
Beta strandi49 – 513Combined sources
Beta strandi54 – 607Combined sources
Beta strandi68 – 725Combined sources
Helixi74 – 763Combined sources
Beta strandi79 – 813Combined sources
Beta strandi83 – 875Combined sources
Helixi89 – 11325Combined sources
Turni114 – 1163Combined sources
Beta strandi119 – 1224Combined sources
Beta strandi127 – 1359Combined sources
Helixi140 – 1434Combined sources
Helixi144 – 15714Combined sources
Beta strandi160 – 1689Combined sources
Beta strandi170 – 1745Combined sources
Helixi190 – 1923Combined sources
Beta strandi196 – 20510Combined sources
Beta strandi208 – 2158Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2E1BX-ray2.70A1-216[»]
ProteinModelPortaliO57848.
SMRiO57848. Positions 1-216.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO57848.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG01254. Archaea.
COG2872. LUCA.
HOGENOMiHOG000230779.
KOiK01872.
OMAiVWHVVAE.

Family and domain databases

InterProiIPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR009000. Transl_B-barrel.
IPR012947. tRNA_SAD.
[Graphical view]
PfamiPF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
SMARTiSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF55186. SSF55186. 1 hit.
PROSITEiPS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O57848-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MINMTRKLYY EDAYLKEAKG RVLEIRDNAI LLDQTIFYPT GGGQPHDRGT
60 70 80 90 100
INGVEVLDVY KDEEGNVWHV VKEPEKFKVG DEVELKIDWD YRYKLMRIHT
110 120 130 140 150
GLHLLEHVLN EVLGEGNWQL VGSGMSVEKG RYDIAYPENL NKYKEQIISL
160 170 180 190 200
FNKYVDEGGE VKIWWEGDRR YTQIRDFEVI PCGGTHVKDI KEIGHIKKLK
210
RSSIGRGKQR LEMWLE
Length:216
Mass (Da):25,299
Last modified:August 1, 1998 - v1
Checksum:i4B4D94685C47EF32
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29177.1.
PIRiB71231.

Genome annotation databases

EnsemblBacteriaiBAA29177; BAA29177; BAA29177.
KEGGipho:PH0108.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29177.1.
PIRiB71231.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2E1BX-ray2.70A1-216[»]
ProteinModelPortaliO57848.
SMRiO57848. Positions 1-216.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH0108.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA29177; BAA29177; BAA29177.
KEGGipho:PH0108.

Phylogenomic databases

eggNOGiarCOG01254. Archaea.
COG2872. LUCA.
HOGENOMiHOG000230779.
KOiK01872.
OMAiVWHVVAE.

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-109-MONOMER.
BRENDAi3.1.1.29. 5244.

Miscellaneous databases

EvolutionaryTraceiO57848.

Family and domain databases

InterProiIPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR009000. Transl_B-barrel.
IPR012947. tRNA_SAD.
[Graphical view]
PfamiPF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
SMARTiSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF55186. SSF55186. 1 hit.
PROSITEiPS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.
  2. "Structure of the AlaX-M trans-editing enzyme from Pyrococcus horikoshii."
    Fukunaga R., Yokoyama S.
    Acta Crystallogr. D 63:390-400(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS), FUNCTION AS TRNA(ALA) EDITING PROTEIN, SUBUNIT, COFACTOR.
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Entry informationi

Entry nameiALAXM_PYRHO
AccessioniPrimary (citable) accession number: O57848
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 2, 2010
Last sequence update: August 1, 1998
Last modified: July 6, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.