O57848 (ALAXM_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanyl-tRNA editing protein AlaX-M
Short name=AlaX-M

Alternative name(s):
Alanyl-tRNA deacylase AlaX-M

Gene names

Name:	alaXM
Ordered Locus Names:	PH0108

Organism

Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]

Taxonomic identifier

70601 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	216 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions in trans to edit the amino acid moiety from mischarged charged Gly-tRNA(Ala) and Ser-tRNA(Ala). Ref.2
Cofactor	Binds 1 zinc ion per subunit. Ref.2
Subunit structure	Monomer. Ref.2
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-M subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro alanine-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aminoacyl-tRNA editing activity Inferred from direct assay Ref.2. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 216

216

Alanyl-tRNA editing protein AlaX-M

PRO_0000391648

Sites

Metal binding

99

1

Zinc

Metal binding

103

1

Zinc

Metal binding

182

1

Zinc

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

216

Helix Strand Turn

Details...

Beta strand

56 – 60

5

Beta strand

68 – 72

5

Helix

74 – 76

3

Helix

89 – 99

11

Helix

103 – 112

10

Beta strand

132 – 135

4

Helix

140 – 143

4

Helix

144 – 156

13

Beta strand

161 – 165

5

Beta strand

170 – 174

5

Helix

190 – 192

3

Beta strand

196 – 203

8

Beta strand

209 – 215

7

Sequences

	Sequence		Length	Mass (Da)
	O57848 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 4B4D94685C47EF32	FASTA	216	25,299
	10 20 30 40 50 60 MINMTRKLYY EDAYLKEAKG RVLEIRDNAI LLDQTIFYPT GGGQPHDRGT INGVEVLDVY 70 80 90 100 110 120 KDEEGNVWHV VKEPEKFKVG DEVELKIDWD YRYKLMRIHT GLHLLEHVLN EVLGEGNWQL 130 140 150 160 170 180 VGSGMSVEKG RYDIAYPENL NKYKEQIISL FNKYVDEGGE VKIWWEGDRR YTQIRDFEVI 190 200 210 PCGGTHVKDI KEIGHIKKLK RSSIGRGKQR LEMWLE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3." Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H. DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.
[2]	"Structure of the AlaX-M trans-editing enzyme from Pyrococcus horikoshii." Fukunaga R., Yokoyama S. Acta Crystallogr. D 63:390-400(2007) [PubMed: 17327676] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS), FUNCTION AS TRNA(ALA) EDITING PROTEIN, SUBUNIT, COFACTOR. Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BA000001 Genomic DNA. Translation: BAA29177.1.

PIR

B71231.

RefSeq

NP_142120.1. NC_000961.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2E1B	X-ray	2.70	A	1-216	[»]

ProteinModelPortal

O57848.

SMR

O57848. Positions 1-216.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBPYRT00000000546; EBPYRP00000000546; EBPYRG00000000546.

GeneID

1444008.

GenomeReviews

Gene locus PH0108 in contig BA000001_GR.

KEGG

pho:PH0108.

NMPDR

fig|70601.1.peg.108.

Organism-specific databases

CMR

Search...

Phylogenomic databases

GeneTree

EBGT00050000022658.

HOGENOM

HBG691293.

OMA

MRLHFAA.

PhylomeDB

O57848.

ProtClustDB

CLSK253141.

Enzyme and pathway databases

BioCyc

PHOR70601:PH0108-MONOMER.

Family and domain databases

InterPro

IPR002318. Ala-tRNA-synth_IIc.
IPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR012947. tRNA_SAD.
[Graphical view]

KO

K07050.

PANTHER

PTHR11777:SF6. PTHR11777:SF6. 1 hit.

Pfam

PF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]

SMART

SM00863. tRNA_SAD. 1 hit.
[Graphical view]

SUPFAM

SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.

PROSITE

PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ALAXM_PYRHO

Accession

Primary (citable) accession number: O57848

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 2, 2010
Last sequence update:	August 1, 1998
Last modified:	December 14, 2011
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program