Reviewed, UniProtKB/Swiss-Prot O57840 (ALF1_PYRHO)

Names and origin

Protein names

Recommended name:
    Fructose-bisphosphate aldolase class 1
    EC=4.1.2.13
Alternative name(s):
    Fructose-biphosphate aldolase class I
      Short name=FBP aldolase

Gene names

Name:	fba
Ordered Locus Names:	PH0082

Organism

Pyrococcus horikoshii [Complete proteome] [HAMAP]

Taxonomic identifier

53953 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.
Enzyme regulation	Activated by citrate By similarity.
Subunit structure	Homooctamer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the deoC/fbaB aldolase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	fructose-bisphosphate aldolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 281	281	Fructose-bisphosphate aldolase class 1		PRO_0000138950
Sites
	Active site	191	1	Schiff-base intermediate with dihydroxyacetone-P By similarity

Sequences

	Sequence		Length	Mass (Da)
	O57840-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 9AB0E4501E983068	FASTA	281	31,189
	10 20 30 40 50 60 MEALQNLGIR RRLKRFFRRD GRALIFAMDH GFEHGPTDFE PVWEHVNPKV IIRKVVRAGI 70 80 90 100 110 120 DGVMMLPGIA RIAGDEVKPD VGLMIKLTSK TNLRPKPDQL LQSQLAYVED AIKLGADAIA 130 140 150 160 170 180 ATVYWGSPQE DTMIRQFAEI ASYAHDLGYP VVQFAYPRGP YIDEKYGKKE DYRVVMYGAR 190 200 210 220 230 240 AAAESGADMI KTYWTGSKET FAKVVEAAAG VPVLMSGGAK TDNPLDFLKV VWEVIEAGGS 250 260 270 280 GAVVGRNIFQ RENPESMIRA LIRVIHRNED PEEAAKAEGL I

References

[1]

"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3." Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H. DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OT3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000001 Genomic DNA. Translation: BAA29151.1.
PIR	H71227.
RefSeq	NP_142099.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1443984.
GenomeReviews	Gene locus PH0082 in contig BA000001_GR.
KEGG	pho:PH0082.
NMPDR	fig|70601.1.peg.84.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG553580.
OMA	SGADMIK.
Enzyme and pathway databases
BioCyc	PHOR70601:PH0082-MONOMER.
BRENDA	4.1.2.13. 74679.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR002915. DeoC/AroFGH_arch. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01791. DeoC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALF1_PYRHO

Accession

Primary (citable) accession number: O57840

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	January 19, 2010
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)