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Protein

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase

Gene

flpA

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Involved in pre-rRNA and tRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in rRNA and tRNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing, tRNA processing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-50-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferaseUniRule annotation (EC:2.1.1.-UniRule annotation)
Gene namesi
Name:flpAUniRule annotation
Ordered Locus Names:PH0052
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 227227Fibrillarin-like rRNA/tRNA 2'-O-methyltransferasePRO_0000148546Add
BLAST

Interactioni

Subunit structurei

Interacts with nop5. Component of box C/D small ribonucleoprotein (sRNP) particles that contain rpl7ae, FlpA and nop5, plus a guide RNA.UniRule annotation

Protein-protein interaction databases

STRINGi70601.PH0052.

Structurei

Secondary structure

1
227
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 64Combined sources
Beta strandi12 – 165Combined sources
Beta strandi18 – 203Combined sources
Beta strandi22 – 276Combined sources
Beta strandi40 – 434Combined sources
Beta strandi46 – 505Combined sources
Turni53 – 553Combined sources
Helixi57 – 637Combined sources
Beta strandi76 – 805Combined sources
Turni81 – 833Combined sources
Helixi87 – 959Combined sources
Beta strandi99 – 1068Combined sources
Helixi108 – 11811Combined sources
Beta strandi124 – 1285Combined sources
Helixi134 – 1374Combined sources
Turni138 – 1403Combined sources
Beta strandi144 – 1496Combined sources
Helixi156 – 16712Combined sources
Beta strandi168 – 17912Combined sources
Helixi180 – 1823Combined sources
Helixi189 – 20113Combined sources
Beta strandi204 – 2118Combined sources
Turni213 – 2153Combined sources
Beta strandi216 – 22510Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1G8AX-ray1.40A1-227[»]
ProteinModelPortaliO57811.
SMRiO57811. Positions 1-227.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO57811.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni86 – 872S-adenosyl-L-methionine bindingUniRule annotation
Regioni105 – 1062S-adenosyl-L-methionine bindingUniRule annotation
Regioni130 – 1312S-adenosyl-L-methionine bindingUniRule annotation
Regioni150 – 1534S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the methyltransferase superfamily. Fibrillarin family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00078. Archaea.
COG1889. LUCA.
HOGENOMiHOG000106741.
KOiK04795.
OMAiEYREWNL.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00351. RNA_methyltransf_FlpA.
InterProiIPR000692. Fibrillarin.
IPR020813. Fibrillarin_CS.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01269. Fibrillarin. 1 hit.
[Graphical view]
PIRSFiPIRSF006540. Nop17p. 1 hit.
PRINTSiPR00052. FIBRILLARIN.
SMARTiSM01206. Fibrillarin. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00566. FIBRILLARIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O57811-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVEVKKHKFP GVYTVIDDDG SERIATKNLV PGQRVYGERV IKWEGEEYRI
60 70 80 90 100
WNPNRSKLGA AIMNGLKNFP IKPGKSVLYL GIASGTTASH VSDIVGWEGK
110 120 130 140 150
IFGIEFSPRV LRELVPIVEE RRNIVPILGD ATKPEEYRAL VPKVDVIFED
160 170 180 190 200
VAQPTQAKIL IDNAEVYLKR GGYGMIAVKS RSIDVTKEPE QVFREVEREL
210 220
SEYFEVIERL NLEPYEKDHA LFVVRKT
Length:227
Mass (Da):25,828
Last modified:August 1, 1998 - v1
Checksum:i1B634D4DC15103E8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29120.1.
PIRiA71224.
RefSeqiWP_010884167.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA29120; BAA29120; BAA29120.
GeneIDi1443949.
KEGGipho:PH0052.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29120.1.
PIRiA71224.
RefSeqiWP_010884167.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1G8AX-ray1.40A1-227[»]
ProteinModelPortaliO57811.
SMRiO57811. Positions 1-227.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH0052.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA29120; BAA29120; BAA29120.
GeneIDi1443949.
KEGGipho:PH0052.

Phylogenomic databases

eggNOGiarCOG00078. Archaea.
COG1889. LUCA.
HOGENOMiHOG000106741.
KOiK04795.
OMAiEYREWNL.

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-50-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO57811.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00351. RNA_methyltransf_FlpA.
InterProiIPR000692. Fibrillarin.
IPR020813. Fibrillarin_CS.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01269. Fibrillarin. 1 hit.
[Graphical view]
PIRSFiPIRSF006540. Nop17p. 1 hit.
PRINTSiPR00052. FIBRILLARIN.
SMARTiSM01206. Fibrillarin. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00566. FIBRILLARIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.
  2. "A structural approach to gene function and structure quality for Pyrococcus horikoshii fibrillarin."
    Berkeley structural genomics center (BSGC)
    Submitted (JAN-2005) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.4 ANGSTROMS).

Entry informationi

Entry nameiFLPA_PYRHO
AccessioniPrimary (citable) accession number: O57811
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 1, 1998
Last modified: May 11, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.