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Protein

Quinolinate synthase A

Gene

nadA

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate.UniRule annotation

Catalytic activityi

Glycerone phosphate + iminosuccinate = pyridine-2,3-dicarboxylate + 2 H2O + phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes quinolinate from iminoaspartate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Quinolinate synthase A (nadA)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes quinolinate from iminoaspartate, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei21Iminoaspartate1
Binding sitei38Iminoaspartate; via amide nitrogen1
Binding sitei109Iminoaspartate1
Binding sitei126Iminoaspartate1
Binding sitei196Iminoaspartate1
Binding sitei213Iminoaspartate1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BRENDAi2.5.1.72. 5244.
UniPathwayiUPA00253; UER00327.

Names & Taxonomyi

Protein namesi
Recommended name:
Quinolinate synthase AUniRule annotation (EC:2.5.1.72UniRule annotation)
Gene namesi
Name:nadAUniRule annotation
Ordered Locus Names:PH0013
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001558101 – 300Quinolinate synthase AAdd BLAST300

Interactioni

Protein-protein interaction databases

STRINGi70601.PH0013.

Structurei

Secondary structure

1300
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 13Combined sources11
Beta strandi16 – 21Combined sources6
Helixi26 – 29Combined sources4
Beta strandi33 – 36Combined sources4
Helixi38 – 44Combined sources7
Helixi45 – 47Combined sources3
Beta strandi50 – 57Combined sources8
Helixi59 – 68Combined sources10
Beta strandi72 – 75Combined sources4
Helixi86 – 88Combined sources3
Helixi91 – 100Combined sources10
Beta strandi106 – 112Combined sources7
Helixi114 – 117Combined sources4
Beta strandi121 – 124Combined sources4
Turni126 – 128Combined sources3
Helixi129 – 135Combined sources7
Beta strandi139 – 145Combined sources7
Helixi147 – 157Combined sources11
Beta strandi160 – 167Combined sources8
Helixi173 – 175Combined sources3
Helixi178 – 187Combined sources10
Beta strandi192 – 195Combined sources4
Helixi201 – 204Combined sources4
Beta strandi208 – 210Combined sources3
Helixi213 – 219Combined sources7
Helixi220 – 222Combined sources3
Beta strandi224 – 230Combined sources7
Helixi233 – 241Combined sources9
Beta strandi245 – 250Combined sources6
Helixi259 – 261Combined sources3
Helixi264 – 273Combined sources10
Helixi282 – 298Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WZUX-ray2.00A1-300[»]
4ZK6X-ray1.90A/B1-300[»]
5KTMX-ray1.44A1-300[»]
5KTNX-ray1.34A1-300[»]
5KTOX-ray1.44A1-300[»]
5KTPX-ray1.54A1-300[»]
5KTRX-ray1.34A1-300[»]
5KTSX-ray1.34A1-300[»]
5KTTX-ray1.50A1-300[»]
ProteinModelPortaliO57767.
SMRiO57767.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO57767.

Family & Domainsi

Sequence similaritiesi

Belongs to the quinolinate synthase A family. Type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04459. Archaea.
COG0379. LUCA.
HOGENOMiHOG000222770.
KOiK03517.
OMAiIAHPECE.

Family and domain databases

Gene3Di3.90.870.10. 1 hit.
HAMAPiMF_00568. NadA_type2. 1 hit.
InterProiIPR017945. DHBP_synth_RibB-like_a/b_dom.
IPR003473. NadA.
IPR023066. Quinolinate_synth_type2.
[Graphical view]
PfamiPF02445. NadA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00550. nadA. 1 hit.

Sequencei

Sequence statusi: Complete.

O57767-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLVEEILRL KEERNAIILA HNYQLPEVQD IADFIGDSLE LARRATRVDA
60 70 80 90 100
DVIVFAGVDF MAETAKILNP DKVVLIPSRE ATCAMANMLK VEHILEAKRK
110 120 130 140 150
YPNAPVVLYV NSTAEAKAYA DVTVTSANAV EVVKKLDSDV VIFGPDKNLA
160 170 180 190 200
HYVAKMTGKK IIPVPSKGHC YVHQKFTLDD VERAKKLHPN AKLMIHPECI
210 220 230 240 250
PEVQEKADII ASTGGMIKRA CEWDEWVVFT EREMVYRLRK LYPQKKFYPA
260 270 280 290 300
REDAFCIGMK AITLKNIYES LKDMKYKVEV PEEIARKARK AIERMLEMSK
Length:300
Mass (Da):34,136
Last modified:August 1, 1998 - v1
Checksum:i35C27FD410C343E5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29081.1.
PIRiB71219.
RefSeqiWP_010884133.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA29081; BAA29081; BAA29081.
GeneIDi1443915.
KEGGipho:PH0013.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29081.1.
PIRiB71219.
RefSeqiWP_010884133.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WZUX-ray2.00A1-300[»]
4ZK6X-ray1.90A/B1-300[»]
5KTMX-ray1.44A1-300[»]
5KTNX-ray1.34A1-300[»]
5KTOX-ray1.44A1-300[»]
5KTPX-ray1.54A1-300[»]
5KTRX-ray1.34A1-300[»]
5KTSX-ray1.34A1-300[»]
5KTTX-ray1.50A1-300[»]
ProteinModelPortaliO57767.
SMRiO57767.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH0013.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA29081; BAA29081; BAA29081.
GeneIDi1443915.
KEGGipho:PH0013.

Phylogenomic databases

eggNOGiarCOG04459. Archaea.
COG0379. LUCA.
HOGENOMiHOG000222770.
KOiK03517.
OMAiIAHPECE.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00327.
BRENDAi2.5.1.72. 5244.

Miscellaneous databases

EvolutionaryTraceiO57767.

Family and domain databases

Gene3Di3.90.870.10. 1 hit.
HAMAPiMF_00568. NadA_type2. 1 hit.
InterProiIPR017945. DHBP_synth_RibB-like_a/b_dom.
IPR003473. NadA.
IPR023066. Quinolinate_synth_type2.
[Graphical view]
PfamiPF02445. NadA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00550. nadA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNADA_PYRHO
AccessioniPrimary (citable) accession number: O57767
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: August 1, 1998
Last modified: November 30, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.