Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Quinolinate synthase A

Gene

nadA

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate.UniRule annotation

Catalytic activityi

Glycerone phosphate + iminosuccinate = pyridine-2,3-dicarboxylate + 2 H2O + phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes quinolinate from iminoaspartate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Quinolinate synthase A (nadA)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes quinolinate from iminoaspartate, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei21 – 211Iminoaspartate
Binding sitei38 – 381Iminoaspartate; via amide nitrogen
Binding sitei109 – 1091Iminoaspartate
Binding sitei126 – 1261Iminoaspartate
Binding sitei196 – 1961Iminoaspartate
Binding sitei213 – 2131Iminoaspartate

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-16-MONOMER.
BRENDAi2.5.1.72. 5244.
UniPathwayiUPA00253; UER00327.

Names & Taxonomyi

Protein namesi
Recommended name:
Quinolinate synthase AUniRule annotation (EC:2.5.1.72UniRule annotation)
Gene namesi
Name:nadAUniRule annotation
Ordered Locus Names:PH0013
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 300300Quinolinate synthase APRO_0000155810Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi70601.PH0013.

Structurei

Secondary structure

1
300
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 1412Combined sources
Beta strandi16 – 216Combined sources
Helixi26 – 316Combined sources
Beta strandi33 – 364Combined sources
Helixi38 – 458Combined sources
Beta strandi49 – 579Combined sources
Helixi59 – 6810Combined sources
Beta strandi72 – 754Combined sources
Helixi92 – 1009Combined sources
Beta strandi106 – 1127Combined sources
Helixi114 – 1174Combined sources
Beta strandi121 – 1244Combined sources
Turni126 – 1283Combined sources
Helixi129 – 1346Combined sources
Beta strandi139 – 1457Combined sources
Helixi147 – 15711Combined sources
Beta strandi160 – 1634Combined sources
Helixi178 – 18710Combined sources
Beta strandi192 – 1954Combined sources
Helixi201 – 2055Combined sources
Beta strandi208 – 2103Combined sources
Helixi213 – 2197Combined sources
Helixi220 – 2223Combined sources
Beta strandi224 – 2307Combined sources
Helixi233 – 2419Combined sources
Beta strandi245 – 2495Combined sources
Turni250 – 2534Combined sources
Helixi264 – 27310Combined sources
Helixi282 – 29716Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WZUX-ray2.00A1-300[»]
ProteinModelPortaliO57767.
SMRiO57767. Positions 1-298.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO57767.

Family & Domainsi

Sequence similaritiesi

Belongs to the quinolinate synthase A family. Type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04459. Archaea.
COG0379. LUCA.
HOGENOMiHOG000222770.
KOiK03517.
OMAiIAHPECE.

Family and domain databases

HAMAPiMF_00568. NadA_type2. 1 hit.
InterProiIPR003473. NadA.
IPR023066. Quinolinate_synth_type2.
[Graphical view]
PfamiPF02445. NadA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00550. nadA. 1 hit.

Sequencei

Sequence statusi: Complete.

O57767-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLVEEILRL KEERNAIILA HNYQLPEVQD IADFIGDSLE LARRATRVDA
60 70 80 90 100
DVIVFAGVDF MAETAKILNP DKVVLIPSRE ATCAMANMLK VEHILEAKRK
110 120 130 140 150
YPNAPVVLYV NSTAEAKAYA DVTVTSANAV EVVKKLDSDV VIFGPDKNLA
160 170 180 190 200
HYVAKMTGKK IIPVPSKGHC YVHQKFTLDD VERAKKLHPN AKLMIHPECI
210 220 230 240 250
PEVQEKADII ASTGGMIKRA CEWDEWVVFT EREMVYRLRK LYPQKKFYPA
260 270 280 290 300
REDAFCIGMK AITLKNIYES LKDMKYKVEV PEEIARKARK AIERMLEMSK
Length:300
Mass (Da):34,136
Last modified:August 1, 1998 - v1
Checksum:i35C27FD410C343E5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29081.1.
PIRiB71219.
RefSeqiWP_010884133.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA29081; BAA29081; BAA29081.
GeneIDi1443915.
KEGGipho:PH0013.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA29081.1.
PIRiB71219.
RefSeqiWP_010884133.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WZUX-ray2.00A1-300[»]
ProteinModelPortaliO57767.
SMRiO57767. Positions 1-298.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH0013.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA29081; BAA29081; BAA29081.
GeneIDi1443915.
KEGGipho:PH0013.

Phylogenomic databases

eggNOGiarCOG04459. Archaea.
COG0379. LUCA.
HOGENOMiHOG000222770.
KOiK03517.
OMAiIAHPECE.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00327.
BioCyciPHOR70601:GJWR-16-MONOMER.
BRENDAi2.5.1.72. 5244.

Miscellaneous databases

EvolutionaryTraceiO57767.

Family and domain databases

HAMAPiMF_00568. NadA_type2. 1 hit.
InterProiIPR003473. NadA.
IPR023066. Quinolinate_synth_type2.
[Graphical view]
PfamiPF02445. NadA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00550. nadA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNADA_PYRHO
AccessioniPrimary (citable) accession number: O57767
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: August 1, 1998
Last modified: December 9, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.