Reviewed, UniProtKB/Swiss-Prot O57765 (NADB_PYRHO)

Names and origin

Protein names

Recommended name:
    L-aspartate oxidase
      Short name=LASPO
    EC=1.4.3.16
Alternative name(s):
    Quinolinate synthetase B

Gene names

Name:	nadB
Ordered Locus Names:	PH0015

Organism

Pyrococcus horikoshii [Complete proteome] [HAMAP]

Taxonomic identifier

53953 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of L-aspartate to iminoaspartate.
Catalytic activity	L-aspartate + O2 = iminosuccinate + H2O2.
Cofactor	FAD.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (oxidase route): step 1/1.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-aspartate oxidase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	L-aspartate oxidase		PRO_0000184410
Regions
	Nucleotide binding	7 – 21	15	FAD Potential
Sites
	Active site	213	1	By similarity
	Active site	230	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O57765-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 6D5504A7EFAD738D	FASTA	464	51,296
	10 20 30 40 50 60 MMEMRVGIVG GGLAGLTAAI ALAEKGFDVS IIGPRSTDSN SYLAQAGIAL PLLEGDSIRI 70 80 90 100 110 120 HVLDTIKAGK YINDEEIVWN VISKSSEAHD FLTSHGVTFT GNELEGGHSY PRIFTIKSET 130 140 150 160 170 180 GKHIIPILEK HARELDVNFI RGFVEEIGIN NGKLAGVFLQ GELLKFDAVV IAAGGFSGLY 190 200 210 220 230 240 RFTAGVKNNI GLLIGDVALK GVPLRDMEFV QFHPTGFIGK RTYLITEAVR GAGAKLVTGD 250 260 270 280 290 300 GERFVNELET RDIVARAIYM KMLEGKGVFL DARGIENFKD RFPYIYSVLR GEGINPEKDL 310 320 330 340 350 360 IPITPVAHYT IGGISVDAFY RTRIKGLYAI GESACNGFHG ANRLASNSLL ECVVSGLEVA 370 380 390 400 410 420 RTISREKPKR EVNDAPYSFN ELGDVDSIRE VLWNHAGIVR DEWSLREGLR KLKEIEVDER 430 440 450 460 LKLVAKAVII SALKREESRG AHYRKDYPFM RKEFEHSSFF YPNV

References

[1]

"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3." Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H. DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OT3.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000001 Genomic DNA. Translation: BAA29083.1.
PIR	D71219.
RefSeq	NP_142038.1.
3D structure databases
HSSP	HSSP built from PDB template 1CHU based on UniProtKB P10902.
ModBase	Search...
Genome annotation databases
GeneID	1443917.
GenomeReviews	Gene locus PH0015 in contig BA000001_GR.
KEGG	pho:PH0015.
NMPDR	fig|70601.1.peg.17.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O57765.
OMA	NENCETS.
Enzyme and pathway databases
BRENDA	1.4.3.16. 74679.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR004112. Fum_Rdtase/Succ_DH_flav_C. IPR005288. NadB. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
TIGRFAMs	TIGR00551. nadB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADB_PYRHO

Accession

Primary (citable) accession number: O57765

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	August 1, 1998
Last modified:	November 3, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)