V-type ATP synthase alpha chain - Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)

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O57728 (VATA_PYRHO) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 94. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
V-type ATP synthase alpha chain
EC=3.6.3.14

Alternative name(s):
V-ATPase subunit A

Cleaved into the following chain:

Endonuclease PI-Pho2
EC=3.1.-.-
Alternative name(s):
Pho AtpA intein
Pho VMA intein

Gene names

Name:	atpA
Ordered Locus Names:	PH1975

Organism

Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) [Complete proteome] [HAMAP]

Taxonomic identifier

70601 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	964 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Produces ATP from ADP in the presence of a proton gradient across the membrane. The archaeal alpha chain is a catalytic subunit. HAMAP MF_00309
Catalytic activity	ATP + H₂O + H⁺(In) = ADP + phosphate + H⁺(Out). HAMAP MF_00309
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the VDE intervening region (intein) followed by peptide ligation Potential. HAMAP MF_00309
Miscellaneous	The intein interrupts the ATP-binding site. HAMAP MF_00309
Sequence similarities	Belongs to the ATPase alpha/beta chains family. Contains 1 DOD-type homing endonuclease domain.

Ontologies

Keywords
Biological process	ATP synthesis Hydrogen ion transport Intron homing Ion transport Transport
Ligand	ATP-binding Nucleotide-binding
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Autocatalytic cleavage Protein splicing
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW ATP hydrolysis coupled proton transport Inferred from electronic annotation. Source: InterPro intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro intron homing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	proton-transporting two-sector ATPase complex, catalytic domain Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW hydrogen ion transporting ATP synthase activity, rotational mechanism Inferred from electronic annotation. Source: InterPro proton-transporting ATPase activity, rotational mechanism Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 240

240

V-type ATP synthase alpha chain, 1st part Potential

PRO_0000002467

Chain

241 – 616

376

Endonuclease PI-Pho2 Potential

PRO_0000002468

Chain

617 – 964

348

V-type ATP synthase alpha chain, 2nd part Potential

PRO_0000002469

Regions

Domain

392 – 518

127

DOD-type homing endonuclease

Secondary structure

964

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

O57728 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 33252C47713BD5E1
Show »

FASTA

964

107,855

        10         20         30         40         50         60 
MVAKGRIIRV TGPLVVADGM KGAKMYEVVR VGELGLIGEI IRLEGDKAVI QVYEETAGVR 

        70         80         90        100        110        120 
PGEPVVGTGA SLSVELGPGL LTSIYDGIQR PLEVIREKTG DFIARGVTAP ALPRDKKWHF 

       130        140        150        160        170        180 
IPKAKVGDKV VGGDIIGEVP ETSIIVHKIM VPPGIEGEIV EIAEEGDYTI EEVIAKVKTP 

       190        200        210        220        230        240 
SGEIKELKMY QRWPVRVKRP YKEKLPPEVP LITGQRVIDT FFPQAKGGTA AIPGPFGSGK 

       250        260        270        280        290        300 
CVDGDTLVLT KEFGLIKIKE LYEKLDGKGR KIVEGNEEWT ELEKPITVYG YKDGKIVEIK 

       310        320        330        340        350        360 
ATHVYKGVSS GMVEIRTRTG RKIKVTPIHR LFTGRVTKDG LILKEVMAMH VKPGDRIAVV 

       370        380        390        400        410        420 
KKIDGGEYIK LDSSNVGEIK VPEILNEELA EFLGYLMANG TLKSGIIEIY CDDESLLERV 

       430        440        450        460        470        480 
NSLSLKLFGV GGRIVQKVDG KALVIQSKPL VDVLRRLGVP EDKKVENWKV PRELLLSPSN 

       490        500        510        520        530        540 
VVRAFVNAYI KGKEEVEITL ASEEGAYELS YLFAKLGIYV TISKSGEYYK VRVSRRGNLD 

       550        560        570        580        590        600 
TIPVEVNGMP KVLPYEDFRK FAKSIGLEEV AENHLQHIIF DEVIDVRYIP EPQEVYDVTT 

       610        620        630        640        650        660 
ETHNFVGGNM PTLLHNTVTQ HQLAKWSDAQ VVIYIGCGER GNEMTDVLEE FPKLKDPKTG 

       670        680        690        700        710        720 
KPLMERTVLI ANTSNMPVAA REASIYTGIT IAEYFRDMGY DVALMADSTS RWAEALREIS 

       730        740        750        760        770        780 
GRLEEMPGEE GYPAYLASKL AEFYERAGRV VTLGSDYRVG SVSVIGAVSP PGGDFSEPVV 

       790        800        810        820        830        840 
QNTLRVVKVF WALDADLARR RHFPAINWLT SYSLYVDAVK DWWHKNIDPE WKAMRDKAMA 

       850        860        870        880        890        900 
LLQKESELQE IVRIVGPDAL PERERAILLV ARMLREDYLQ QDAFDEVDTY CPPEKQVTMM 

       910        920        930        940        950        960 
RVLLNFYDKT MEAINRGVPL EEIAKLPVRE EIGRMKFERD VSKIRSLIDK TNEQFEELFK 


KYGA

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References

[1]

"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3."
Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T.

Kikuchi H.
DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BA000001 Genomic DNA. Translation: BAA31102.1.

PIR

G71213.

RefSeq

NP_143800.1. NC_000961.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1VDZ	X-ray	2.55	A	1-964	[»]
3I4L	X-ray	2.40	A	1-964	[»]
3I72	X-ray	2.47	A	1-964	[»]
3I73	X-ray	2.40	A	1-964	[»]
3IKJ	X-ray	2.40	A	1-964	[»]
3M4Y	X-ray	2.38	A	1-964	[»]
3MFY	X-ray	2.35	A	1-964	[»]
3ND8	X-ray	2.40	A	1-964	[»]
3ND9	X-ray	3.10	A	1-964	[»]
3P20	X-ray	2.85	A	1-964	[»]
3QG1	X-ray	2.95	A	1-964	[»]
3QIA	X-ray	2.60	A	1-964	[»]
3QJY	X-ray	2.35	A	1-964	[»]

ProteinModelPortal

O57728.

SMR

O57728. Positions 60-287, 617-964.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBPYRT00000001975; EBPYRP00000001975; EBPYRG00000001975.

GeneID

1442821.

GenomeReviews

Gene locus PH1975 in contig BA000001_GR.

KEGG

pho:PH1975.

NMPDR

fig|70601.1.peg.1935.

Organism-specific databases

CMR

Search...

Phylogenomic databases

GeneTree

EBGT00050000022151.

HOGENOM

HBG288114.

OMA

HNCGERG.

PhylomeDB

O57728.

Enzyme and pathway databases

BioCyc

PHOR70601:PH1975-MONOMER.

Family and domain databases

HAMAP

MF_00309. ATP_synth_A_arch. Divergent sequence.
[Tree]

InterPro

IPR020003. ATPase_a/bsu_AS.
IPR018118. ATPase_F1/A1-cplx_a/bsu_N.
IPR000793. ATPase_F1/V1/A1-cplx_a/bsu_C.
IPR004100. ATPase_F1/V1/A1-cplx_a/bsu_N.
IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
IPR024034. ATPase_F1_bsu/V1_C.
IPR003586. Hedgehog_hint_C.
IPR003587. Hedgehog_hint_N.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR022878. V-ATPase_su_A/alpha.
[Graphical view]

Gene3D

G3DSA:1.10.1140.10. G3DSA:1.10.1140.10. 1 hit.

K02117.

Pfam

PF00006. ATP-synt_ab. 2 hits.
PF00306. ATP-synt_ab_C. 1 hit.
PF02874. ATP-synt_ab_N. 1 hit.
[Graphical view]

SMART

SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]

SUPFAM

SSF47917. ATPase_a/b_C. 1 hit.
SSF50615. ATPase_a/b_N. 1 hit.

TIGRFAMs

TIGR01443. Intein_Cterm. 1 hit.
TIGR01445. Intein_Nterm. 1 hit.

PROSITE

PS00152. ATPASE_ALPHA_BETA. 1 hit.
PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

VATA_PYRHO

Accession

Primary (citable) accession number: O57728

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	August 1, 1998
Last modified:	December 14, 2011
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Intein-containing proteins

List of intein-containing protein entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Cleaved into the following chain:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents