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Protein

V-type ATP synthase alpha chain

Gene

atpA

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Produces ATP from ADP in the presence of a proton gradient across the membrane. The archaeal alpha chain is a catalytic subunit.

Catalytic activityi

ATP + H2O + H+(In) = ADP + phosphate + H+(Out).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

ATP synthesis, Hydrogen ion transport, Intron homing, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1979-MONOMER.
BRENDAi3.6.3.14. 5244.

Names & Taxonomyi

Protein namesi
Recommended name:
V-type ATP synthase alpha chain (EC:3.6.3.14)
Alternative name(s):
V-ATPase subunit A
Cleaved into the following chain:
Alternative name(s):
Pho AtpA intein
Pho VMA intein
Gene namesi
Name:atpA
Ordered Locus Names:PH1975
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 240240V-type ATP synthase alpha chain, 1st partSequence analysisPRO_0000002467Add
BLAST
Chaini241 – 616376Endonuclease PI-Pho2Sequence analysisPRO_0000002468Add
BLAST
Chaini617 – 964348V-type ATP synthase alpha chain, 2nd partSequence analysisPRO_0000002469Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the VDE intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Protein-protein interaction databases

STRINGi70601.PH1975.

Structurei

Secondary structure

1
964
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi20 – 223Combined sources
Beta strandi30 – 323Combined sources
Beta strandi37 – 415Combined sources
Beta strandi44 – 463Combined sources
Beta strandi49 – 546Combined sources
Beta strandi61 – 644Combined sources
Beta strandi66 – 705Combined sources
Beta strandi73 – 775Combined sources
Beta strandi84 – 863Combined sources
Beta strandi91 – 944Combined sources
Beta strandi100 – 1023Combined sources
Beta strandi114 – 1163Combined sources
Beta strandi118 – 1225Combined sources
Beta strandi135 – 1406Combined sources
Beta strandi142 – 1443Combined sources
Beta strandi146 – 1505Combined sources
Beta strandi157 – 1626Combined sources
Beta strandi165 – 1684Combined sources
Beta strandi172 – 1787Combined sources
Beta strandi184 – 1896Combined sources
Beta strandi191 – 1944Combined sources
Beta strandi202 – 2054Combined sources
Beta strandi209 – 2113Combined sources
Helixi216 – 2216Combined sources
Beta strandi229 – 2324Combined sources
Beta strandi236 – 2394Combined sources
Helixi617 – 62610Combined sources
Beta strandi630 – 6367Combined sources
Beta strandi639 – 6424Combined sources
Helixi643 – 6508Combined sources
Turni651 – 6533Combined sources
Turni657 – 6593Combined sources
Beta strandi660 – 6623Combined sources
Helixi663 – 6664Combined sources
Beta strandi667 – 6715Combined sources
Beta strandi674 – 6763Combined sources
Helixi678 – 69720Combined sources
Beta strandi701 – 7077Combined sources
Turni709 – 7113Combined sources
Beta strandi713 – 7197Combined sources
Helixi735 – 74410Combined sources
Beta strandi748 – 7514Combined sources
Beta strandi753 – 7564Combined sources
Beta strandi759 – 7679Combined sources
Beta strandi771 – 7755Combined sources
Helixi779 – 7868Combined sources
Beta strandi788 – 7903Combined sources
Helixi795 – 7995Combined sources
Turni808 – 8103Combined sources
Beta strandi812 – 8143Combined sources
Helixi816 – 82611Combined sources
Helixi831 – 85525Combined sources
Helixi857 – 8593Combined sources
Helixi862 – 87716Combined sources
Turni886 – 8894Combined sources
Helixi893 – 91523Combined sources
Helixi920 – 9245Combined sources
Helixi927 – 9326Combined sources
Helixi933 – 9375Combined sources
Helixi941 – 9455Combined sources
Helixi947 – 96216Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VDZX-ray2.55A1-964[»]
3I4LX-ray2.40A1-964[»]
3I72X-ray2.47A1-964[»]
3I73X-ray2.40A1-964[»]
3IKJX-ray2.40A1-964[»]
3M4YX-ray2.38A1-964[»]
3MFYX-ray2.35A1-964[»]
3ND8X-ray2.40A1-964[»]
3ND9X-ray3.10A1-964[»]
3P20X-ray2.85A1-964[»]
3QG1X-ray2.95A1-238[»]
A617-964[»]
3QIAX-ray2.60A1-964[»]
3QJYX-ray2.35A1-964[»]
3SDZX-ray2.53A1-964[»]
3SE0X-ray2.62A1-964[»]
ProteinModelPortaliO57728.
SMRiO57728. Positions 60-287, 617-964.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO57728.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini392 – 518127DOD-type homing endonucleaseAdd
BLAST

Sequence similaritiesi

Belongs to the ATPase alpha/beta chains family.Curated

Phylogenomic databases

eggNOGiarCOG00868. Archaea.
arCOG03154. Archaea.
COG1155. LUCA.
HOGENOMiHOG000161057.
KOiK02117.
OMAiFGSGKCV.

Family and domain databases

Gene3Di1.10.1140.10. 1 hit.
2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00309. ATP_synth_A_arch. 1 hit.
InterProiIPR031686. ATP-synt_a_Xtn.
IPR020003. ATPase_a/bsu_AS.
IPR000793. ATPase_F1/V1/A1-cplx_a/bsu_C.
IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
IPR004100. ATPase_F1_a/bsu_N.
IPR024034. ATPase_F1_bsu/V1_C.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR030934. Intein_C.
IPR004042. Intein_endonuc.
IPR006141. Intein_N.
IPR027417. P-loop_NTPase.
IPR022878. V-ATPase_asu.
[Graphical view]
PfamiPF00006. ATP-synt_ab. 2 hits.
PF02874. ATP-synt_ab_N. 1 hit.
PF16886. ATP-synt_ab_Xtn. 1 hit.
[Graphical view]
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF47917. SSF47917. 1 hit.
SSF50615. SSF50615. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 2 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEiPS00152. ATPASE_ALPHA_BETA. 1 hit.
PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O57728-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAKGRIIRV TGPLVVADGM KGAKMYEVVR VGELGLIGEI IRLEGDKAVI
60 70 80 90 100
QVYEETAGVR PGEPVVGTGA SLSVELGPGL LTSIYDGIQR PLEVIREKTG
110 120 130 140 150
DFIARGVTAP ALPRDKKWHF IPKAKVGDKV VGGDIIGEVP ETSIIVHKIM
160 170 180 190 200
VPPGIEGEIV EIAEEGDYTI EEVIAKVKTP SGEIKELKMY QRWPVRVKRP
210 220 230 240 250
YKEKLPPEVP LITGQRVIDT FFPQAKGGTA AIPGPFGSGK CVDGDTLVLT
260 270 280 290 300
KEFGLIKIKE LYEKLDGKGR KIVEGNEEWT ELEKPITVYG YKDGKIVEIK
310 320 330 340 350
ATHVYKGVSS GMVEIRTRTG RKIKVTPIHR LFTGRVTKDG LILKEVMAMH
360 370 380 390 400
VKPGDRIAVV KKIDGGEYIK LDSSNVGEIK VPEILNEELA EFLGYLMANG
410 420 430 440 450
TLKSGIIEIY CDDESLLERV NSLSLKLFGV GGRIVQKVDG KALVIQSKPL
460 470 480 490 500
VDVLRRLGVP EDKKVENWKV PRELLLSPSN VVRAFVNAYI KGKEEVEITL
510 520 530 540 550
ASEEGAYELS YLFAKLGIYV TISKSGEYYK VRVSRRGNLD TIPVEVNGMP
560 570 580 590 600
KVLPYEDFRK FAKSIGLEEV AENHLQHIIF DEVIDVRYIP EPQEVYDVTT
610 620 630 640 650
ETHNFVGGNM PTLLHNTVTQ HQLAKWSDAQ VVIYIGCGER GNEMTDVLEE
660 670 680 690 700
FPKLKDPKTG KPLMERTVLI ANTSNMPVAA REASIYTGIT IAEYFRDMGY
710 720 730 740 750
DVALMADSTS RWAEALREIS GRLEEMPGEE GYPAYLASKL AEFYERAGRV
760 770 780 790 800
VTLGSDYRVG SVSVIGAVSP PGGDFSEPVV QNTLRVVKVF WALDADLARR
810 820 830 840 850
RHFPAINWLT SYSLYVDAVK DWWHKNIDPE WKAMRDKAMA LLQKESELQE
860 870 880 890 900
IVRIVGPDAL PERERAILLV ARMLREDYLQ QDAFDEVDTY CPPEKQVTMM
910 920 930 940 950
RVLLNFYDKT MEAINRGVPL EEIAKLPVRE EIGRMKFERD VSKIRSLIDK
960
TNEQFEELFK KYGA
Length:964
Mass (Da):107,855
Last modified:August 1, 1998 - v1
Checksum:i33252C47713BD5E1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA31102.1.
PIRiG71213.
RefSeqiWP_010886039.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA31102; BAA31102; BAA31102.
GeneIDi1442821.
KEGGipho:PH1975.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA31102.1.
PIRiG71213.
RefSeqiWP_010886039.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VDZX-ray2.55A1-964[»]
3I4LX-ray2.40A1-964[»]
3I72X-ray2.47A1-964[»]
3I73X-ray2.40A1-964[»]
3IKJX-ray2.40A1-964[»]
3M4YX-ray2.38A1-964[»]
3MFYX-ray2.35A1-964[»]
3ND8X-ray2.40A1-964[»]
3ND9X-ray3.10A1-964[»]
3P20X-ray2.85A1-964[»]
3QG1X-ray2.95A1-238[»]
A617-964[»]
3QIAX-ray2.60A1-964[»]
3QJYX-ray2.35A1-964[»]
3SDZX-ray2.53A1-964[»]
3SE0X-ray2.62A1-964[»]
ProteinModelPortaliO57728.
SMRiO57728. Positions 60-287, 617-964.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH1975.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA31102; BAA31102; BAA31102.
GeneIDi1442821.
KEGGipho:PH1975.

Phylogenomic databases

eggNOGiarCOG00868. Archaea.
arCOG03154. Archaea.
COG1155. LUCA.
HOGENOMiHOG000161057.
KOiK02117.
OMAiFGSGKCV.

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1979-MONOMER.
BRENDAi3.6.3.14. 5244.

Miscellaneous databases

EvolutionaryTraceiO57728.

Family and domain databases

Gene3Di1.10.1140.10. 1 hit.
2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00309. ATP_synth_A_arch. 1 hit.
InterProiIPR031686. ATP-synt_a_Xtn.
IPR020003. ATPase_a/bsu_AS.
IPR000793. ATPase_F1/V1/A1-cplx_a/bsu_C.
IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
IPR004100. ATPase_F1_a/bsu_N.
IPR024034. ATPase_F1_bsu/V1_C.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR030934. Intein_C.
IPR004042. Intein_endonuc.
IPR006141. Intein_N.
IPR027417. P-loop_NTPase.
IPR022878. V-ATPase_asu.
[Graphical view]
PfamiPF00006. ATP-synt_ab. 2 hits.
PF02874. ATP-synt_ab_N. 1 hit.
PF16886. ATP-synt_ab_Xtn. 1 hit.
[Graphical view]
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF47917. SSF47917. 1 hit.
SSF50615. SSF50615. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 2 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEiPS00152. ATPASE_ALPHA_BETA. 1 hit.
PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVATA_PYRHO
AccessioniPrimary (citable) accession number: O57728
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: August 1, 1998
Last modified: September 7, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The intein interrupts the ATP-binding site.

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.