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Protein

V-type ATP synthase alpha chain

Gene

atpA

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Produces ATP from ADP in the presence of a proton gradient across the membrane. The archaeal alpha chain is a catalytic subunit.

Miscellaneous

The intein interrupts the ATP-binding site.

Catalytic activityi

ATP + H2O + H+(In) = ADP + phosphate + H+(Out).

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processATP synthesis, Hydrogen ion transport, Intron homing, Ion transport, Transport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:G1G39-1913-MONOMER
BRENDAi3.6.3.14 5244

Names & Taxonomyi

Protein namesi
Recommended name:
V-type ATP synthase alpha chain (EC:3.6.3.14)
Alternative name(s):
V-ATPase subunit A
Cleaved into the following chain:
Alternative name(s):
Pho AtpA intein
Pho VMA intein
Gene namesi
Name:atpA
Ordered Locus Names:PH1975
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000024671 – 240V-type ATP synthase alpha chain, 1st partSequence analysisAdd BLAST240
ChainiPRO_0000002468241 – 616Endonuclease PI-Pho2Sequence analysisAdd BLAST376
ChainiPRO_0000002469617 – 964V-type ATP synthase alpha chain, 2nd partSequence analysisAdd BLAST348

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the VDE intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Proteomic databases

PRIDEiO57728

Interactioni

Protein-protein interaction databases

STRINGi70601.PH1975

Structurei

Secondary structure

1964
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi20 – 22Combined sources3
Beta strandi30 – 32Combined sources3
Beta strandi37 – 41Combined sources5
Beta strandi44 – 46Combined sources3
Beta strandi49 – 54Combined sources6
Beta strandi61 – 64Combined sources4
Beta strandi66 – 70Combined sources5
Beta strandi73 – 77Combined sources5
Beta strandi84 – 86Combined sources3
Beta strandi91 – 94Combined sources4
Beta strandi100 – 102Combined sources3
Beta strandi114 – 116Combined sources3
Beta strandi118 – 122Combined sources5
Beta strandi135 – 140Combined sources6
Beta strandi142 – 144Combined sources3
Beta strandi146 – 150Combined sources5
Beta strandi157 – 162Combined sources6
Beta strandi165 – 168Combined sources4
Beta strandi172 – 178Combined sources7
Beta strandi184 – 189Combined sources6
Beta strandi191 – 194Combined sources4
Beta strandi202 – 205Combined sources4
Beta strandi209 – 211Combined sources3
Helixi216 – 221Combined sources6
Beta strandi229 – 232Combined sources4
Beta strandi236 – 239Combined sources4
Helixi617 – 626Combined sources10
Beta strandi630 – 636Combined sources7
Beta strandi639 – 642Combined sources4
Helixi643 – 650Combined sources8
Turni651 – 653Combined sources3
Turni657 – 659Combined sources3
Beta strandi660 – 662Combined sources3
Helixi663 – 666Combined sources4
Beta strandi667 – 671Combined sources5
Beta strandi674 – 676Combined sources3
Helixi678 – 697Combined sources20
Beta strandi701 – 707Combined sources7
Turni709 – 711Combined sources3
Beta strandi713 – 719Combined sources7
Helixi735 – 744Combined sources10
Beta strandi748 – 751Combined sources4
Beta strandi753 – 756Combined sources4
Beta strandi759 – 767Combined sources9
Beta strandi771 – 775Combined sources5
Helixi779 – 786Combined sources8
Beta strandi788 – 790Combined sources3
Helixi795 – 799Combined sources5
Turni808 – 810Combined sources3
Beta strandi812 – 814Combined sources3
Helixi816 – 826Combined sources11
Helixi831 – 855Combined sources25
Helixi857 – 859Combined sources3
Helixi862 – 877Combined sources16
Turni886 – 889Combined sources4
Helixi893 – 915Combined sources23
Helixi920 – 924Combined sources5
Helixi927 – 932Combined sources6
Helixi933 – 937Combined sources5
Helixi941 – 945Combined sources5
Helixi947 – 962Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VDZX-ray2.55A1-964[»]
3I4LX-ray2.40A1-964[»]
3I72X-ray2.47A1-964[»]
3I73X-ray2.40A1-964[»]
3IKJX-ray2.40A1-964[»]
3M4YX-ray2.38A1-964[»]
3MFYX-ray2.35A1-964[»]
3ND8X-ray2.40A1-964[»]
3ND9X-ray3.10A1-964[»]
3P20X-ray2.85A1-964[»]
3QG1X-ray2.95A1-238[»]
A617-964[»]
3QIAX-ray2.60A1-964[»]
3QJYX-ray2.35A1-964[»]
3SDZX-ray2.53A1-964[»]
3SE0X-ray2.62A1-964[»]
5X09X-ray2.35A51-964[»]
ProteinModelPortaliO57728
SMRiO57728
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO57728

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini392 – 518DOD-type homing endonucleaseAdd BLAST127

Sequence similaritiesi

Belongs to the ATPase alpha/beta chains family.Curated

Phylogenomic databases

eggNOGiarCOG00868 Archaea
arCOG03154 Archaea
COG1155 LUCA
HOGENOMiHOG000161057
KOiK02117
OMAiHNTVTQH
OrthoDBiPOG093Z00V1

Family and domain databases

Gene3Di1.10.1140.10, 1 hit
2.40.30.20, 1 hit
3.10.28.10, 1 hit
HAMAPiMF_00309 ATP_synth_A_arch, 1 hit
InterProiView protein in InterPro
IPR031686 ATP-synth_a_Xtn
IPR023366 ATP_synth_asu-like_sf
IPR020003 ATPase_a/bsu_AS
IPR004100 ATPase_F1/V1/A1_a/bsu_N
IPR036121 ATPase_F1/V1/A1_a/bsu_N_sf
IPR000194 ATPase_F1/V1/A1_a/bsu_nucl-bd
IPR024034 ATPase_F1/V1_b/a_C
IPR003586 Hint_dom_C
IPR003587 Hint_dom_N
IPR036844 Hint_dom_sf
IPR027434 Homing_endonucl
IPR030934 Intein_C
IPR004042 Intein_endonuc
IPR006141 Intein_N
IPR027417 P-loop_NTPase
IPR022878 V-ATPase_asu
PANTHERiPTHR43607 PTHR43607, 2 hits
PfamiView protein in Pfam
PF00006 ATP-synt_ab, 1 hit
PF02874 ATP-synt_ab_N, 1 hit
PF16886 ATP-synt_ab_Xtn, 1 hit
SMARTiView protein in SMART
SM00305 HintC, 1 hit
SM00306 HintN, 1 hit
SUPFAMiSSF50615 SSF50615, 1 hit
SSF51294 SSF51294, 2 hits
SSF52540 SSF52540, 2 hits
SSF55608 SSF55608, 1 hit
TIGRFAMsiTIGR01443 intein_Cterm, 1 hit
TIGR01445 intein_Nterm, 1 hit
PROSITEiView protein in PROSITE
PS00152 ATPASE_ALPHA_BETA, 1 hit
PS50818 INTEIN_C_TER, 1 hit
PS50819 INTEIN_ENDONUCLEASE, 1 hit
PS50817 INTEIN_N_TER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O57728-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAKGRIIRV TGPLVVADGM KGAKMYEVVR VGELGLIGEI IRLEGDKAVI
60 70 80 90 100
QVYEETAGVR PGEPVVGTGA SLSVELGPGL LTSIYDGIQR PLEVIREKTG
110 120 130 140 150
DFIARGVTAP ALPRDKKWHF IPKAKVGDKV VGGDIIGEVP ETSIIVHKIM
160 170 180 190 200
VPPGIEGEIV EIAEEGDYTI EEVIAKVKTP SGEIKELKMY QRWPVRVKRP
210 220 230 240 250
YKEKLPPEVP LITGQRVIDT FFPQAKGGTA AIPGPFGSGK CVDGDTLVLT
260 270 280 290 300
KEFGLIKIKE LYEKLDGKGR KIVEGNEEWT ELEKPITVYG YKDGKIVEIK
310 320 330 340 350
ATHVYKGVSS GMVEIRTRTG RKIKVTPIHR LFTGRVTKDG LILKEVMAMH
360 370 380 390 400
VKPGDRIAVV KKIDGGEYIK LDSSNVGEIK VPEILNEELA EFLGYLMANG
410 420 430 440 450
TLKSGIIEIY CDDESLLERV NSLSLKLFGV GGRIVQKVDG KALVIQSKPL
460 470 480 490 500
VDVLRRLGVP EDKKVENWKV PRELLLSPSN VVRAFVNAYI KGKEEVEITL
510 520 530 540 550
ASEEGAYELS YLFAKLGIYV TISKSGEYYK VRVSRRGNLD TIPVEVNGMP
560 570 580 590 600
KVLPYEDFRK FAKSIGLEEV AENHLQHIIF DEVIDVRYIP EPQEVYDVTT
610 620 630 640 650
ETHNFVGGNM PTLLHNTVTQ HQLAKWSDAQ VVIYIGCGER GNEMTDVLEE
660 670 680 690 700
FPKLKDPKTG KPLMERTVLI ANTSNMPVAA REASIYTGIT IAEYFRDMGY
710 720 730 740 750
DVALMADSTS RWAEALREIS GRLEEMPGEE GYPAYLASKL AEFYERAGRV
760 770 780 790 800
VTLGSDYRVG SVSVIGAVSP PGGDFSEPVV QNTLRVVKVF WALDADLARR
810 820 830 840 850
RHFPAINWLT SYSLYVDAVK DWWHKNIDPE WKAMRDKAMA LLQKESELQE
860 870 880 890 900
IVRIVGPDAL PERERAILLV ARMLREDYLQ QDAFDEVDTY CPPEKQVTMM
910 920 930 940 950
RVLLNFYDKT MEAINRGVPL EEIAKLPVRE EIGRMKFERD VSKIRSLIDK
960
TNEQFEELFK KYGA
Length:964
Mass (Da):107,855
Last modified:August 1, 1998 - v1
Checksum:i33252C47713BD5E1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA Translation: BAA31102.1
PIRiG71213
RefSeqiWP_010886039.1, NC_000961.1

Genome annotation databases

EnsemblBacteriaiBAA31102; BAA31102; BAA31102
GeneIDi1442821
KEGGipho:PH1975

Similar proteinsi

Entry informationi

Entry nameiVATA_PYRHO
AccessioniPrimary (citable) accession number: O57728
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: August 1, 1998
Last modified: April 25, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health