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Protein

tRNA N6-adenosine threonylcarbamoyltransferase

Gene

kae1

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Kae1 likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity.UniRule annotation

Catalytic activityi

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N6-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 1 Fe2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi107IronUniRule annotation1
Metal bindingi111IronUniRule annotation1
Metal bindingi127IronUniRule annotation1
Binding sitei159SubstrateUniRule annotation1
Binding sitei172Substrate; via amide nitrogenUniRule annotation1
Binding sitei176SubstrateUniRule annotation1
Binding sitei257SubstrateUniRule annotation1
Metal bindingi285IronUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processtRNA processing
LigandIron, Metal-binding

Enzyme and pathway databases

BioCyciPHOR70601:G1G39-1923-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.3.1.234UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthaseUniRule annotation
Short name:
t(6)A synthaseUniRule annotation
t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
Gene namesi
Name:kae1UniRule annotation
Ordered Locus Names:PH1987
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000969821 – 324tRNA N6-adenosine threonylcarbamoyltransferaseAdd BLAST324

Proteomic databases

PRIDEiO57716

Interactioni

Subunit structurei

Monomer. Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.UniRule annotation

Protein-protein interaction databases

STRINGi70601.PH1987

Structurei

3D structure databases

ProteinModelPortaliO57716
SMRiO57716
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni127 – 131Substrate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the KAE1 / TsaD family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01183 Archaea
COG0533 LUCA
HOGENOMiHOG000109569
KOiK01409
OMAiAHAGAEM
OrthoDBiPOG093Z05SV

Family and domain databases

HAMAPiMF_01446 Kae1, 1 hit
InterProiView protein in InterPro
IPR000905 Gcp-like_dom
IPR034680 Kae1/OSGEP
IPR017861 KAE1/TsaD
IPR022449 Kae1_arc
IPR017860 Peptidase_M22_CS
PANTHERiPTHR11735 PTHR11735, 1 hit
PTHR11735:SF14 PTHR11735:SF14, 1 hit
PfamiView protein in Pfam
PF00814 Peptidase_M22, 1 hit
PRINTSiPR00789 OSIALOPTASE
TIGRFAMsiTIGR03722 arch_KAE1, 1 hit
TIGR00329 gcp_kae1, 1 hit
PROSITEiView protein in PROSITE
PS01016 GLYCOPROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

O57716-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLALGIEGTA HTLGIGIVSE KKVLANVFDT LTTEKGGIHP KEAAEHHARL
60 70 80 90 100
MKPLLKKALE KAGISMDDID VIAFSQGPGL GPALRVVATA ARALAIRYNK
110 120 130 140 150
PIVGVNHCIA HVEITKMFGI KDPVGLYVSG GNTQVLALEG GRYRVFGETL
160 170 180 190 200
DIGIGNAIDV FARELGLGFP GGPKLEKLAE KGKNYIDLPY AVKGMDLSFS
210 220 230 240 250
GLLTEAIRKY RSGKFRVEDL AYSFQETAFA ALVEVTERAL AHTEKKEVVL
260 270 280 290 300
VGGVAANNRL REMLKIMAED RGVKFFVPPY DLCRDNGAMI AYTGLRMYKA
310 320
GISFPLEKTI VKQKFRTDEV EITW
Length:324
Mass (Da):35,306
Last modified:August 1, 1998 - v1
Checksum:iF8D100D12666FBB6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA Translation: BAA31114.1
PIRiC71215
RefSeqiWP_010886049.1, NC_000961.1

Genome annotation databases

EnsemblBacteriaiBAA31114; BAA31114; BAA31114
GeneIDi1442831
KEGGipho:PH1987

Similar proteinsi

Entry informationi

Entry nameiKAE1_PYRHO
AccessioniPrimary (citable) accession number: O57716
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: May 23, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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