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Protein

tRNA N6-adenosine threonylcarbamoyltransferase

Gene

kae1

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Kae1 likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity.UniRule annotation

Catalytic activityi

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 1 Fe2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi107 – 1071IronUniRule annotation
Metal bindingi111 – 1111IronUniRule annotation
Metal bindingi127 – 1271IronUniRule annotation
Binding sitei159 – 1591SubstrateUniRule annotation
Binding sitei172 – 1721Substrate; via amide nitrogenUniRule annotation
Binding sitei176 – 1761SubstrateUniRule annotation
Binding sitei257 – 2571SubstrateUniRule annotation
Metal bindingi285 – 2851IronUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1989-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.3.1.234UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthaseUniRule annotation
Short name:
t(6)A synthaseUniRule annotation
t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
Gene namesi
Name:kae1UniRule annotation
Ordered Locus Names:PH1987
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 324324tRNA N6-adenosine threonylcarbamoyltransferasePRO_0000096982Add
BLAST

Interactioni

Subunit structurei

Monomer. Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.UniRule annotation

Protein-protein interaction databases

STRINGi70601.PH1987.

Structurei

3D structure databases

ProteinModelPortaliO57716.
SMRiO57716. Positions 1-324.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni127 – 1315Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the KAE1 / TsaD family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01183. Archaea.
COG0533. LUCA.
HOGENOMiHOG000109569.
KOiK01409.
OMAiERYCIDN.

Family and domain databases

HAMAPiMF_01446. Kae1.
InterProiIPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/TsaD.
IPR017860. Peptidase_M22_CS.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O57716-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLALGIEGTA HTLGIGIVSE KKVLANVFDT LTTEKGGIHP KEAAEHHARL
60 70 80 90 100
MKPLLKKALE KAGISMDDID VIAFSQGPGL GPALRVVATA ARALAIRYNK
110 120 130 140 150
PIVGVNHCIA HVEITKMFGI KDPVGLYVSG GNTQVLALEG GRYRVFGETL
160 170 180 190 200
DIGIGNAIDV FARELGLGFP GGPKLEKLAE KGKNYIDLPY AVKGMDLSFS
210 220 230 240 250
GLLTEAIRKY RSGKFRVEDL AYSFQETAFA ALVEVTERAL AHTEKKEVVL
260 270 280 290 300
VGGVAANNRL REMLKIMAED RGVKFFVPPY DLCRDNGAMI AYTGLRMYKA
310 320
GISFPLEKTI VKQKFRTDEV EITW
Length:324
Mass (Da):35,306
Last modified:August 1, 1998 - v1
Checksum:iF8D100D12666FBB6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA31114.1.
PIRiC71215.
RefSeqiWP_010886049.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA31114; BAA31114; BAA31114.
GeneIDi1442831.
KEGGipho:PH1987.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA31114.1.
PIRiC71215.
RefSeqiWP_010886049.1. NC_000961.1.

3D structure databases

ProteinModelPortaliO57716.
SMRiO57716. Positions 1-324.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH1987.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA31114; BAA31114; BAA31114.
GeneIDi1442831.
KEGGipho:PH1987.

Phylogenomic databases

eggNOGiarCOG01183. Archaea.
COG0533. LUCA.
HOGENOMiHOG000109569.
KOiK01409.
OMAiERYCIDN.

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1989-MONOMER.

Family and domain databases

HAMAPiMF_01446. Kae1.
InterProiIPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/TsaD.
IPR017860. Peptidase_M22_CS.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Entry informationi

Entry nameiKAE1_PYRHO
AccessioniPrimary (citable) accession number: O57716
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: July 6, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.