Reviewed, UniProtKB/Swiss-Prot O57708 (GCSPA_PYRHO)

Names and origin

Protein names

Recommended name:
    Probable glycine dehydrogenase [decarboxylating] subunit 1
    EC=1.4.4.2
Alternative name(s):
    Glycine decarboxylase subunit 1
    Glycine cleavage system P-protein subunit 1

Gene names

Name:	gcvPA
Ordered Locus Names:	PH1995

Organism

Pyrococcus horikoshii [Complete proteome] [HAMAP]

Taxonomic identifier

53953 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein By similarity.
Catalytic activity	Glycine + H-protein-lipoyllysine = H-protein-S-aminomethyldihydrolipoyllysine + CO2. HAMAP MF_00712
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H. In this organism, the P 'protein' is an heterodimer of two subunits By similarity.
Sequence similarities	Belongs to the gcvP family. N-terminal subunit subfamily.

Ontologies

Keywords
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine decarboxylation via glycine cleavage system Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glycine dehydrogenase (decarboxylating) activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 449	449	Probable glycine dehydrogenase [decarboxylating] subunit 1 HAMAP MF_00712		PRO_0000166986

Sequences

	Sequence		Length	Mass (Da)
	O57708-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: C7DE610E8A0254DC	FASTA	449	50,357
	10 20 30 40 50 60 MGNHYIPNSA HKEEMLKEIG LSSIEELFAD IPEKFIKEEL NLPEGKSEYE VFLEMNEILE 70 80 90 100 110 120 KNKTVLEMPT FLGAGTYFHY IPAHVKHLIE RSEFLTSYTP YQPEISQGML QALFEYQSLI 130 140 150 160 170 180 AELVGLPVVN SSMYDWGTAM AEAALMTVRL HKGKRKKFVV PKHTHPERLQ VLRTYTRGPE 190 200 210 220 230 240 VEIVTVNWNE RGQVDVEDLK EKVKDAAGVY IEIPNFFGLL EEEIREVGEI AHEAGAYFVV 250 260 270 280 290 300 GVDPTILGIV EAPGELGADI VVGEASYFGN PMNFGGPRAG IFAVKNDMKL IRQMPGRIIG 310 320 330 340 350 360 MTKDAEGKRA FVMTLQTREQ HIRRAKATSN ICSNEALVAV AAAIHIASLG PKGIRELGEV 370 380 390 400 410 420 ILKNTAYLKK RLSEVAEIPF DGVNFKDVLV RFDKPYREIH EELLKRNIHG GYYVGSHFPE 430 440 LGEAALFAAT ETTRKEWVDA LISALKEVI

References

[1]

"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3." Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H. DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OT3.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000001 Genomic DNA. Translation: BAA31122.1.
PIR	C71216.
RefSeq	NP_143817.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1442838.
GenomeReviews	Gene locus PH1995 in contig BA000001_GR.
KEGG	pho:PH1995.
NMPDR	fig|70601.1.peg.1952.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O57708.
OMA	O57708. VANASMY.
Enzyme and pathway databases
BRENDA	1.4.4.2. 74679.
Family and domain databases
HAMAP	MF_00712. [Tree]
InterPro	IPR003437. GDC-P. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11773. GDC-P. 1 hit.
Pfam	PF02347. GDC-P. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCSPA_PYRHO

Accession

Primary (citable) accession number: O57708

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2003
Last sequence update:	August 1, 1998
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)