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Protein

50S ribosomal protein L12

Gene

rpl12

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors. The stalk complex of P.horikoshii binds to E.coli large subunits and confers on them the ability to interact with eukaryotic elongation factors. Each succesive L12 dimer bound along the P0 spine increases the GTPase activity of elongation factors and increases translation by reconsituted ribosomes.UniRule annotation1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-2000-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
50S ribosomal protein L12UniRule annotation
Alternative name(s):
Acidic ribosomal protein P1 homolog
Gene namesi
Name:rpl12UniRule annotation
Ordered Locus Names:PH1998
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10810850S ribosomal protein L12PRO_0000157635Add
BLAST

Interactioni

Subunit structurei

Part of the 50S ribosomal subunit. Homodimer, it forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Forms a heptameric P0(L12)2(L12)2(L12)2 complex, where P0 forms an elongated spine to which the L12 dimers bind in a sequential fashion.2 Publications

Protein-protein interaction databases

STRINGi70601.PH1998.

Structurei

Secondary structure

1
108
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 1211Combined sources
Helixi19 – 2810Combined sources
Helixi35 – 4511Combined sources
Helixi50 – 578Combined sources
Helixi84 – 10623Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3A1YX-ray2.13A/B/C/D/E/F1-58[»]
3WY9X-ray2.30C/D77-108[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO57705.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L12P family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04287. Archaea.
COG2058. LUCA.
HOGENOMiHOG000229898.
KOiK02869.
OMAiEYIYAAM.

Family and domain databases

HAMAPiMF_01478. Ribosomal_L12_arch. 1 hit.
InterProiIPR027534. Ribosomal_L12.
IPR022295. Ribosomal_L12_arc.
[Graphical view]
TIGRFAMsiTIGR03685. ribo_P1_arch. 1 hit.

Sequencei

Sequence statusi: Complete.

O57705-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEYVYAALLL HSVGKEINEE NLKAVLQAAG VEPEEARIKA LVAALEGVNI
60 70 80 90 100
DEVIEKAAMP VAVAAAPAAA PAEAGGEEKK EEEKKEEEEK EEEVSEEEAL

AGLSALFG
Length:108
Mass (Da):11,403
Last modified:June 20, 2001 - v2
Checksum:iCE3E35D79FCAAA82
GO

Sequence cautioni

The sequence BAA31125 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA31125.1. Different initiation.
PIRiF71216.
RefSeqiWP_010886059.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA31125; BAA31125; BAA31125.
GeneIDi1442841.
KEGGipho:PH1998.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA31125.1. Different initiation.
PIRiF71216.
RefSeqiWP_010886059.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3A1YX-ray2.13A/B/C/D/E/F1-58[»]
3WY9X-ray2.30C/D77-108[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH1998.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA31125; BAA31125; BAA31125.
GeneIDi1442841.
KEGGipho:PH1998.

Phylogenomic databases

eggNOGiarCOG04287. Archaea.
COG2058. LUCA.
HOGENOMiHOG000229898.
KOiK02869.
OMAiEYIYAAM.

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-2000-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO57705.

Family and domain databases

HAMAPiMF_01478. Ribosomal_L12_arch. 1 hit.
InterProiIPR027534. Ribosomal_L12.
IPR022295. Ribosomal_L12_arc.
[Graphical view]
TIGRFAMsiTIGR03685. ribo_P1_arch. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRL12_PYRHO
AccessioniPrimary (citable) accession number: O57705
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 20, 2001
Last modified: September 7, 2016
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.