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Protein

50S ribosomal protein L12

Gene

rpl12

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors. The stalk complex of P.horikoshii binds to E.coli large subunits and confers on them the ability to interact with eukaryotic elongation factors. Each succesive L12 dimer bound along the P0 spine increases the GTPase activity of elongation factors and increases translation by reconsituted ribosomes.UniRule annotation1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciPHOR70601:G1G39-1935-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
50S ribosomal protein L12UniRule annotation
Alternative name(s):
Acidic ribosomal protein P1 homolog
Gene namesi
Name:rpl12UniRule annotation
Ordered Locus Names:PH1998
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001576351 – 10850S ribosomal protein L12Add BLAST108

Interactioni

Subunit structurei

Part of the 50S ribosomal subunit. Homodimer, it forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Forms a heptameric P0(L12)2(L12)2(L12)2 complex, where P0 forms an elongated spine to which the L12 dimers bind in a sequential fashion.2 Publications

Protein-protein interaction databases

STRINGi70601.PH1998

Structurei

Secondary structure

1108
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 12Combined sources11
Helixi19 – 28Combined sources10
Helixi35 – 45Combined sources11
Helixi50 – 57Combined sources8
Helixi84 – 106Combined sources23

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3A1YX-ray2.13A/B/C/D/E/F1-58[»]
3WY9X-ray2.30C/D77-108[»]
5H7LX-ray3.10E/G98-108[»]
SMRiO57705
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO57705

Family & Domainsi

Sequence similaritiesi

Belongs to the eukaryotic ribosomal protein P1/P2 family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04287 Archaea
COG2058 LUCA
HOGENOMiHOG000229898
KOiK02869
OMAiQELLCTY
OrthoDBiPOG093Z0NOH

Family and domain databases

CDDicd05832 Ribosomal_L12p, 1 hit
Gene3Di1.10.10.1410, 1 hit
HAMAPiMF_01478 Ribosomal_L12_arch, 1 hit
InterProiView protein in InterPro
IPR038716 P1/P2_N_sf
IPR027534 Ribosomal_L12
IPR022295 Ribosomal_L12_arc
PANTHERiPTHR21141:SF73 PTHR21141:SF73, 1 hit
TIGRFAMsiTIGR03685 ribo_P1_arch, 1 hit

Sequencei

Sequence statusi: Complete.

O57705-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEYVYAALLL HSVGKEINEE NLKAVLQAAG VEPEEARIKA LVAALEGVNI
60 70 80 90 100
DEVIEKAAMP VAVAAAPAAA PAEAGGEEKK EEEKKEEEEK EEEVSEEEAL

AGLSALFG
Length:108
Mass (Da):11,403
Last modified:June 20, 2001 - v2
Checksum:iCE3E35D79FCAAA82
GO

Sequence cautioni

The sequence BAA31125 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA Translation: BAA31125.1 Different initiation.
PIRiF71216
RefSeqiWP_010886059.1, NC_000961.1

Genome annotation databases

EnsemblBacteriaiBAA31125; BAA31125; BAA31125
GeneIDi1442841
KEGGipho:PH1998

Similar proteinsi

Entry informationi

Entry nameiRL12_PYRHO
AccessioniPrimary (citable) accession number: O57705
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 20, 2001
Last modified: April 25, 2018
This is version 90 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health