O57693 (O57693_THETE) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Glyceraldehyde-3-phosphate dehydrogenase (NADP+) EMBL CAA71651.1
EC=1.2.1.9 EMBL CAA71651.1

Gene names

Name:

GAPN EMBL CAA71651.1

Organism

Thermoproteus tenax EMBL CAA71651.1

Taxonomic identifier

2271 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Thermoproteus

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	NAD PDB 1UXT NADP PDB 1UXR PDB 1UXN PDB 1UXP PDB 1KY8 PDB 1UXU PDB 1UXV PDB 1UXQ Nucleotide-binding PDB 1UXR PDB 1UXN PDB 1UXP PDB 1KY8 PDB 1UXT PDB 1UXU PDB 1UXV PDB 1UXQ
Molecular function	Oxidoreductase EMBL CAA71651.1
Technical term	3D-structure PDB 1UXR PDB 1UXN PDB 1UXP PDB 1KY8 PDB 1UXT PDB 1UXU PDB 1UXV PDB 1UXQ
Gene Ontology (GO)
Molecular function	glyceraldehyde-3-phosphate dehydrogenase (NADP+) (non-phosphorylating) activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	132 – 134	3	AMP PDB 1UXN PDB 1UXP PDB 1UXU PDB 1UXV
	Nucleotide binding	141 – 142	2	AMP PDB 1UXN PDB 1UXP PDB 1UXU PDB 1UXV
	Nucleotide binding	154 – 155	2	AMP PDB 1UXN PDB 1UXP PDB 1UXU
	Nucleotide binding	165 – 167	3	NADP PDB 1UXR PDB 1UXN PDB 1UXP PDB 1KY8 PDB 1UXU PDB 1UXV PDB 1UXQ
	Nucleotide binding	191 – 194	4	NADP PDB 1UXR PDB 1UXN PDB 1UXP PDB 1KY8 PDB 1UXU PDB 1UXV PDB 1UXQ
	Nucleotide binding	243 – 244	2	NAD PDB 1UXT
	Nucleotide binding	243 – 244	2	NADP PDB 1UXR PDB 1UXN PDB 1UXP PDB 1KY8 PDB 1UXU PDB 1UXV PDB 1UXQ
	Nucleotide binding	263 – 265	3	NAD PDB 1UXT
	Nucleotide binding	263 – 265	3	NADP PDB 1UXR PDB 1UXN PDB 1UXP PDB 1KY8 PDB 1UXV PDB 1UXQ
	Nucleotide binding	395 – 397	3	NADP PDB 1UXR PDB 1UXN PDB 1UXP PDB 1KY8 PDB 1UXU PDB 1UXV PDB 1UXQ
	Region	141 – 142	2	Fructose-6-phosphate binding PDB 1UXR
	Region	141 – 142	2	Glucose-1-phosphate binding PDB 1UXT PDB 1UXQ
	Region	154 – 155	2	Glucose-1-phosphate binding PDB 1UXT PDB 1UXQ
Sites
	Binding site	72	1	AMP PDB 1UXN PDB 1UXP PDB 1UXU PDB 1UXV
	Binding site	72	1	Fructose-6-phosphate PDB 1UXR
	Binding site	72	1	Glucose-1-phosphate PDB 1UXT PDB 1UXQ
	Binding site	79	1	AMP PDB 1UXN PDB 1UXP PDB 1UXU PDB 1UXV
	Binding site	79	1	Fructose-6-phosphate PDB 1UXR
	Binding site	79	1	Glucose-1-phosphate PDB 1UXT PDB 1UXQ
	Binding site	165	1	NAD; via carbonyl oxygen PDB 1UXT
	Binding site	184	1	Fructose-6-phosphate; via carbonyl oxygen PDB 1UXR
	Binding site	191	1	NAD PDB 1UXT
	Binding site	224	1	NAD PDB 1UXT
	Binding site	224	1	NADP PDB 1UXR PDB 1UXN PDB 1UXP PDB 1KY8 PDB 1UXU PDB 1UXV PDB 1UXQ
	Binding site	228	1	NAD PDB 1UXT
	Binding site	228	1	NADP PDB 1UXR PDB 1UXN PDB 1UXP PDB 1KY8 PDB 1UXU PDB 1UXV PDB 1UXQ
	Binding site	395	1	NAD PDB 1UXT
	Binding site	440	1	AMP PDB 1UXN PDB 1UXP PDB 1UXU PDB 1UXV
	Binding site	440	1	Fructose-6-phosphate PDB 1UXR
	Binding site	440	1	Glucose-1-phosphate PDB 1UXT PDB 1UXQ
	Binding site	498	1	AMP PDB 1UXN PDB 1UXP PDB 1UXU PDB 1UXV
	Binding site	498	1	Fructose-6-phosphate PDB 1UXR
	Binding site	498	1	Glucose-1-phosphate PDB 1UXT PDB 1UXQ

Sequences

	Sequence		Length	Mass (Da)
	O57693 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 22F1C0084282EE1B	FASTA	501	54,090
	10 20 30 40 50 60 MRAGLLEGVI KEKGGVPVYP SYLAGEWGGS GQEIEVKSPI DLATIAKVIS PSREEVERTL 70 80 90 100 110 120 DVLFKRGRWS ARDMPGTERL AVLRKAADII ERNLDVFAEV LVMNAGKPKS AAVGEVKAAV 130 140 150 160 170 180 DRLRLAELDL KKIGGDYIPG DWTYDTLETE GLVRREPLGV VAAITPFNYP LFDAVNKITY 190 200 210 220 230 240 SFIYGNAVVV KPSISDPLPA AMAVKALLDA GFPPDAIALL NLPGKEAEKI VADDRVAAVS 250 260 270 280 290 300 FTGSTEVGER VVKVGGVKQY VMELGGGDPA IVLEDADLDL AADKIARGIY SYAGQRCDAI 310 320 330 340 350 360 KLVLAERPVY GKLVEEVAKR LSSLRVGDPR DPTVDVGPLI SPSAVDEMMA AIEDAVEKGG 370 380 390 400 410 420 RVLAGGRRLG PTYVQPTLVE APADRVKDMV LYKREVFAPV ASAVEVKDLD QAIELANGRP 430 440 450 460 470 480 YGLDAAVFGR DVVKIRRAVR LLEVGAIYIN DMPRHGIGYY PFGGRKKSGV FREGIGYAVE 490 500 AVTAYKTIVF NYKGKGVWKY E

References

[1]	"NAD+-dependent GAPDH from Thermoproteus tenax - the first identified archaeal member of the aldehyde dehydrogenase superfamily is a glycolytic enzyme with unusual regulatory properties." Brunner N.A., Brinkmann H., Siebers B., Hensel R. J. Biol. Chem. 273:6149-6156(1998) [PubMed: 9497334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: KRA 1 EMBL CAA71651.1.
[2]	Brunner N.A. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: KRA 1 EMBL CAA71651.1.
[3]	"The crystal structure of the allosteric non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeum Thermoproteus tenax." Pohl E., Brunner N., Wilmanns M., Hensel R. J. Biol. Chem. 277:19938-19945(2002) [PubMed: 11842090] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) IN COMPLEX WITH NADP.
[4]	"Structural Basis of allosteric regulation and substrate specificity of the non-phosphorylating glyceraldehyde 3-Phosphate dehydrogenase from Thermoproteus tenax." Lorentzen E., Hensel R., Knura T., Ahmed H., Pohl E. J. Mol. Biol. 341:815-828(2004) [PubMed: 15288789] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) IN COMPLEX WITH AMP; FRUCTOSE-6-PHOSPHATE; GLUCOSE-1-PHOSPHATE; NAD AND NADP.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Y10625 Genomic DNA. Translation: CAA71651.1.

PIR

T44939.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KY8	X-ray	2.40	A	1-501	[»]
1UXN	X-ray	2.30	A	1-501	[»]
1UXP	X-ray	2.55	A	1-501	[»]
1UXQ	X-ray	2.40	A	1-501	[»]
1UXR	X-ray	2.30	A	1-501	[»]
1UXT	X-ray	2.20	A	1-501	[»]
1UXU	X-ray	2.25	A	1-501	[»]
1UXV	X-ray	2.35	A	1-501	[»]

ProteinModelPortal

O57693.

SMR

O57693. Positions 3-501.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view]

Gene3D

G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit.
G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.

Pfam

PF00171. Aldedh. 1 hit.
[Graphical view]

SUPFAM

SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.

ProtoNet

Search...

Entry information

Entry name

O57693_THETE

Accession

Primary (citable) accession number: O57693

Entry history

Integrated into UniProtKB/TrEMBL:	August 1, 1998
Last sequence update:	August 1, 1998
Last modified:	October 19, 2011
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)