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Protein

Paired box protein Pax-2-A

Gene

pax2-a

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Probable transcription factor. Involved in kidney development, acting synergistically with lhx1/lim-1 in pronephric morphogenesis during the tailbud stages (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Segmentation polarity protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Paired box protein Pax-2-A
Short name:
xPax-2a
Gene namesi
Name:pax2-a
Synonyms:pax-2aImported
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-486805. pax2.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotationBy similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 497497Paired box protein Pax-2-APRO_0000289132Add
BLAST

Expressioni

Tissue specificityi

Expression becomes spatially localized at mid-gastrula stages and is confined to the nervous system (midbrain, hindbrain, spinal cord), sensory organs (optic vesicle and stalk, otic vesicle), visceral arches, developing excretory system (pronephros, pronephric duct, rectal diverticulum, proctodaeum) and thyroid gland. Splicing does not appear to be tissue-specific and tissues displayed the same spectrum of splice variants.3 Publications

Developmental stagei

Splicing is temporally regulated. Isoform 5 is expressed both maternally and zygotically, whereas isoform 1, isoform 2, isoform 3 and isoform 4 are exclusively zygotic. Expression is highest in embryos from stage 12 on, with expression levels remaining constant throughout embryogenesis.1 Publication

Inductioni

By activin. Weakly by bFGF.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO57685.
SMRiO57685. Positions 19-142.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini16 – 142127PairedPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 paired domain.PROSITE-ProRule annotation

Keywords - Domaini

Paired box

Phylogenomic databases

HOVERGENiHBG009115.
KOiK15608.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR009057. Homeodomain-like.
IPR001523. Paired_dom.
IPR022130. Pax2_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00292. PAX. 1 hit.
PF12403. Pax2_C. 2 hits.
[Graphical view]
PRINTSiPR00027. PAIREDBOX.
SMARTiSM00351. PAX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00034. PAIRED_1. 1 hit.
PS51057. PAIRED_2. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O57685-1) [UniParc]FASTAAdd to basket

Also known as: 41 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDMHCKADPF SAMHPGHGGV NQLGGVFVNG RPLPDVVRQR IVELAHQGVR
60 70 80 90 100
PCDISRQLRV SHGCVSKILG RYYETGSIKP GVIGGSKPKV ATPKVVDKIA
110 120 130 140 150
EYKRQNPTMF AWEIRDRLLA EGICDNDTVP SVSSINRIIR TKVQQPFHPT
160 170 180 190 200
PDGSGTPVGT PGHTLVPSTA SPPVSSASND PVGSYSINGI LGIPRSNGEK
210 220 230 240 250
RKRDEDGSDG SGPNGDSQSS VESLRKHLRA DNFTQQQLEA LDRVFERPSY
260 270 280 290 300
PDVFQTAEHI KSEQASEYSL PALTPGLDEV KSSLSASGNA DLGSNVSGPQ
310 320 330 340 350
SYPVVTESFA SHLYVKQEPH EASLTPFTPS SLASSGLADI QPFQMALTVD
360 370 380 390 400
ASTPTYSSFT HHGPHYGQFG SQPLIAGRDM SSTTLPGYPP HVPPTGQGSY
410 420 430 440 450
PTSTLAGMVP GTNVSVHHSV QPVECCSCLS SSKPCLFHCR TGSGSEFSGN
460 470 480 490
PYSHPQYTTY NEAWRFSNPA LLSSPYYYSA TSRGSAPPTA ATAYDRH
Length:497
Mass (Da):53,204
Last modified:May 29, 2007 - v2
Checksum:i2815A8C329878237
GO
Isoform 2 (identifier: O57685-2) [UniParc]FASTAAdd to basket

Also known as: 11 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     307-376: Missing.
     412-444: Missing.

Show »
Length:394
Mass (Da):42,229
Checksum:iBC81726C6E51EDF2
GO
Isoform 31 Publication (identifier: O57685-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     307-376: Missing.

Show »
Length:427
Mass (Da):45,702
Checksum:i7E6EF01E04E062C1
GO
Isoform 4 (identifier: O57685-4) [UniParc]FASTAAdd to basket

Also known as: 51 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     166-205: Missing.
     307-376: Missing.
     412-444: Missing.

Show »
Length:354
Mass (Da):38,106
Checksum:i059AC72416A555F7
GO
Isoform 5 (identifier: O57685-5) [UniParc]FASTAAdd to basket

Also known as: 71 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     206-376: Missing.
     445-445: S → FLGS

Show »
Length:329
Mass (Da):35,283
Checksum:i81DE1C10F3CED0A4
GO
Isoform 6 (identifier: O57685-6) [UniParc]FASTAAdd to basket

Also known as: 10

The sequence of this isoform differs from the canonical sequence as follows:
     166-376: Missing.
     412-444: Missing.
     445-445: S → FLGS

Note: No experimental confirmation available.
Show »
Length:256
Mass (Da):27,687
Checksum:i4B225AC9EAA7BF6D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti91 – 911A → T in CAA71208 (PubMed:9486533).Curated
Sequence conflicti144 – 1441Q → R in CAA71208 (PubMed:9486533).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei166 – 376211Missing in isoform 6. 1 PublicationVSP_052436Add
BLAST
Alternative sequencei166 – 20540Missing in isoform 4. 1 PublicationVSP_052435Add
BLAST
Alternative sequencei206 – 376171Missing in isoform 5. 1 PublicationVSP_052437Add
BLAST
Alternative sequencei307 – 37670Missing in isoform 2, isoform 3 and isoform 4. 1 PublicationVSP_052438Add
BLAST
Alternative sequencei412 – 44433Missing in isoform 2, isoform 4 and isoform 6. 2 PublicationsVSP_052439Add
BLAST
Alternative sequencei445 – 4451S → FLGS in isoform 5 and isoform 6. 2 PublicationsVSP_052440

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10119 mRNA. Translation: CAA71205.1.
Y10121 mRNA. Translation: CAA71207.1.
Y10122 mRNA. Translation: CAA71208.1.
Y10123 mRNA. Translation: CAA71209.1.
AJ000667 mRNA. Translation: CAA04223.1.
BC055960 mRNA. Translation: AAH55960.1.
RefSeqiNP_001079830.1. NM_001086361.1. [O57685-3]
UniGeneiXl.1130.

Genome annotation databases

GeneIDi379520.
KEGGixla:379520.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10119 mRNA. Translation: CAA71205.1.
Y10121 mRNA. Translation: CAA71207.1.
Y10122 mRNA. Translation: CAA71208.1.
Y10123 mRNA. Translation: CAA71209.1.
AJ000667 mRNA. Translation: CAA04223.1.
BC055960 mRNA. Translation: AAH55960.1.
RefSeqiNP_001079830.1. NM_001086361.1. [O57685-3]
UniGeneiXl.1130.

3D structure databases

ProteinModelPortaliO57685.
SMRiO57685. Positions 19-142.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi379520.
KEGGixla:379520.

Organism-specific databases

CTDi379520.
XenbaseiXB-GENE-486805. pax2.

Phylogenomic databases

HOVERGENiHBG009115.
KOiK15608.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR009057. Homeodomain-like.
IPR001523. Paired_dom.
IPR022130. Pax2_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00292. PAX. 1 hit.
PF12403. Pax2_C. 2 hits.
[Graphical view]
PRINTSiPR00027. PAIREDBOX.
SMARTiSM00351. PAX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00034. PAIRED_1. 1 hit.
PS51057. PAIRED_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Xenopus Pax-2 displays multiple splice forms during embryogenesis and pronephric kidney development."
    Heller N., Braendli A.W.
    Mech. Dev. 69:83-104(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3; 4 AND 5), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
    Tissue: Embryonic headImported and Embryonic kidney1 Publication.
  2. NIH - Xenopus Gene Collection (XGC) project
    Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 6).
    Tissue: EmbryoImported.
  3. "Xenopus Pax-2/5/8 orthologues: novel insights into Pax gene evolution and identification of Pax-8 as the earliest marker for otic and pronephric cell lineages."
    Heller N., Braendli A.W.
    Dev. Genet. 24:208-219(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  4. "Molecular anatomy of placode development in Xenopus laevis."
    Schlosser G., Ahrens K.
    Dev. Biol. 271:439-466(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiPAX2A_XENLA
AccessioniPrimary (citable) accession number: O57685
Secondary accession number(s): O57677
, O57680, O57681, O57684, Q7SZU4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: December 9, 2015
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.