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O57539

- NCOA3_XENLA

UniProt

O57539 - NCOA3_XENLA

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Protein
Nuclear receptor coactivator 3
Gene
ncoa3
Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, probably via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as retinoids (RAR and RXR), thyroid hormone (TR) and orphan nuclear receptor (hepatocyte nuclear receptor 4 (HNF4) and constitutive androstane receptor (CAR)). Displays histone acetyltransferase activity.

Catalytic activityi

Acetyl-CoA + [histone] = CoA + acetyl-[histone].

GO - Molecular functioni

  1. histone acetyltransferase activity Source: UniProtKB-EC
  2. ligand-dependent nuclear receptor transcription coactivator activity Source: InterPro
  3. protein binding Source: IntAct
  4. signal transducer activity Source: InterPro

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: UniProtKB-KW
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Acyltransferase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear receptor coactivator 3 (EC:2.3.1.48)
Alternative name(s):
Retinoid X receptor-interacting coactivator xSRC-3
Gene namesi
Name:ncoa3
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-865628. ncoa3.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity
Note: Mainly cytoplasmic and weakly nuclear By similarity.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi622 – 6221L → A: Weakly impairs interaction with nuclear receptors. 1 Publication
Mutagenesisi683 – 6831L → A: Strongly impairs interaction with nuclear receptors. 1 Publication
Mutagenesisi739 – 7391L → A: Strongly impairs interaction with nuclear receptors. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13911391Nuclear receptor coactivator 3
PRO_0000094409Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei614 – 6141N6-acetyllysine By similarity
Modified residuei617 – 6171N6-acetyllysine By similarity
Modified residuei618 – 6181N6-acetyllysine By similarity

Post-translational modificationi

Phosphorylated and acetylated By similarity.

Keywords - PTMi

Acetylation, Phosphoprotein

Expressioni

Tissue specificityi

Highly expressed in liver and in early stages of oocyte development.

Developmental stagei

Expressed only in early stages of oocyte development. Expression is more prominent in stage I, strongly decreases in stage II and then, gradually disappears.

Interactioni

Subunit structurei

Interacts with the histone acetyltransferase protein EP300.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ncoa3O57539-12EBI-301587,EBI-301595

Structurei

3D structure databases

ProteinModelPortaliO57539.
SMRiO57539. Positions 1036-1082.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 8458bHLH
Add
BLAST
Domaini112 – 18271PAS
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1088 – 1274187Acetyltransferase
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi680 – 6845LXXLL motif 1
Motifi736 – 7405LXXLL motif 2
Motifi1048 – 10525LXXLL motif 3

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi503 – 666164Ser-rich
Add
BLAST
Compositional biasi515 – 5228Poly-Ser
Compositional biasi968 – 9714Poly-Gln
Compositional biasi1241 – 12488Poly-Gln

Domaini

Contains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain).

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

HOVERGENiHBG052583.
KOiK11256.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
4.10.630.10. 2 hits.
InterProiIPR011598. bHLH_dom.
IPR010011. DUF1518.
IPR028818. NCOA3.
IPR009110. Nuc_rcpt_coact.
IPR014920. Nuc_rcpt_coact_Ncoa-typ.
IPR017426. Nuclear_rcpt_coactivator.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR014935. SRC-1.
IPR008955. Src1_rcpt_coact.
[Graphical view]
PANTHERiPTHR10684. PTHR10684. 1 hit.
PTHR10684:SF3. PTHR10684:SF3. 1 hit.
PfamiPF07469. DUF1518. 1 hit.
PF08815. Nuc_rec_co-act. 1 hit.
PF00989. PAS. 1 hit.
PF08832. SRC-1. 1 hit.
[Graphical view]
PIRSFiPIRSF038181. Nuclear_receptor_coactivator. 1 hit.
SMARTiSM00353. HLH. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
SSF55785. SSF55785. 2 hits.
SSF69125. SSF69125. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
PS50112. PAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms may be produced.

Isoform 1 (identifier: O57539-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSGLGENSLD PLASETRKRK PSSCDTPGPG LTCSGEKRRR EQESKYIEEL     50
ADLISANLSD IDNFNVKPDK CAILKETVRQ IRQIKEQGKA SSNDDDVQKA 100
DVSSTGQGVI DKDSLGPLLL QALDGFLYVV NREGSIVFVS ENVTQYLQYK 150
QEDLVNTSVY SILHEEDRKD FLKNLPKSTV NGVPWFSETP RQKSHTFNCR 200
MLVKTSHDHL EDGSNLDARQ RYETMQCFAL SQPRAMIEEG EDLQSCMICV 250
ARRITTAERA FSANPESFIT RHDLTGKVVN IDANSLRSSM RPGFEDTIRR 300
CIQRFLFHSE GQPWTYKRHY QEAYVHGLSE TPLYRFSLAD GTMVTAQTKS 350
KLFRNPVTND PHGFVSTHFL QREQNGYRPN PNPMAQGIRP QMNPNLPNTM 400
NSMPPQAMQQ QNRNYGMGDP NSMAQMQGMR YKSPGNMAPV NQAPGVQQSP 450
YQNNSNYGLN MNSPPHGSPG MNANQPNLMV SPRNRASPKM ASNQFSPVPG 500
MNSPMGSSGN AGGGSFSSSS LSALHAISEG VGSSLLSSLS SPGQKVENNS 550
NMNMPQQGKI CNQDCKSPSG LYCEQGQVES SVCQSSGREH LGEKDVKENI 600
FEGSESQRSQ AESKGHKKLL QLLTCFTEER GQSLMSSSSM DCKDSSNVTS 650
PSGVSSSTSI GVSSTSNLHG SMLQEKHRIL HKLLQNGNSP AEVAKITAEA 700
TGKDVFQETV SSAPCTEATV KREQLSPKKK ENNALLRHLL DKDDWKDPLA 750
KDIKPKVEHM DIKMGSCSSS NVPTSSQDKE VKIKTEPGEE VPGDLDNLDA 800
ILGDLAGSDF YSNSMSSRAS DLGPKQPVFQ DSPTLAMRSP DSMQGSRPPF 850
NRAMSLDSRS STPPVRNVNS FPMLPKQGMI GSPRMMDGQD NFGVMMGSGP 900
NRSMNQHPGG DWAMQNSAVN RLEPPNVGSV GRPGPDYSSA MTRPAMGGNM 950
PGLLTRSNSI PGSRPVMQQQ QHILPMRPND MAMSMGSNPY GQQAPSNPPG 1000
SWPDAIMMNQ GRGGAQNRQL GRNSLDDLLC PPSTVEGQTD EIALLDQLHT 1050
LLSNTDATGL EEIDRALGIP DLVSQGQALE PQPDSYQPQG SPVMIDQKPP 1100
MYGQHYAGQG AAMSAGGFNN MQGQHPPFNT VMGQMNQQQG MHPLQGMHPR 1150
ANLIRPRNNI PKQLRMQLQQ RLQGQQFLNQ NRQALEMKVD PMNPGGAGVM 1200
RPVMQTPVSQ QGFLNAQMVA QKNRELISHQ IRQHRMAMMM QQQQGQPQAF 1250
SPPPNVTASA SMDNPLGGPP MPQAPPQQFS YPPNYGINQQ TDPTFGRVSS 1300
PPNAMMSSRM APSQNPHPQT TQMYPSPDMK GWPSGNMARP NSFPQQQYSH 1350
QTNPATYNMM HMNGNGNHMG QMNINSLPMS GMPMGPDQKY C 1391
Length:1,391
Mass (Da):152,532
Last modified:June 1, 1998 - v1
Checksum:iAD28F5CD934AC33D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF044080 mRNA. Translation: AAC12927.1.
RefSeqiNP_001081732.1. NM_001088263.1. [O57539-1]
UniGeneiXl.268.

Genome annotation databases

GeneIDi398021.
KEGGixla:398021.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF044080 mRNA. Translation: AAC12927.1 .
RefSeqi NP_001081732.1. NM_001088263.1. [O57539-1 ]
UniGenei Xl.268.

3D structure databases

ProteinModelPortali O57539.
SMRi O57539. Positions 1036-1082.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 398021.
KEGGi xla:398021.

Organism-specific databases

CTDi 8202.
Xenbasei XB-GENE-865628. ncoa3.

Phylogenomic databases

HOVERGENi HBG052583.
KOi K11256.

Family and domain databases

Gene3Di 4.10.280.10. 1 hit.
4.10.630.10. 2 hits.
InterProi IPR011598. bHLH_dom.
IPR010011. DUF1518.
IPR028818. NCOA3.
IPR009110. Nuc_rcpt_coact.
IPR014920. Nuc_rcpt_coact_Ncoa-typ.
IPR017426. Nuclear_rcpt_coactivator.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR014935. SRC-1.
IPR008955. Src1_rcpt_coact.
[Graphical view ]
PANTHERi PTHR10684. PTHR10684. 1 hit.
PTHR10684:SF3. PTHR10684:SF3. 1 hit.
Pfami PF07469. DUF1518. 1 hit.
PF08815. Nuc_rec_co-act. 1 hit.
PF00989. PAS. 1 hit.
PF08832. SRC-1. 1 hit.
[Graphical view ]
PIRSFi PIRSF038181. Nuclear_receptor_coactivator. 1 hit.
SMARTi SM00353. HLH. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view ]
SUPFAMi SSF47459. SSF47459. 1 hit.
SSF55785. SSF55785. 2 hits.
SSF69125. SSF69125. 1 hit.
PROSITEi PS50888. BHLH. 1 hit.
PS50112. PAS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of xSRC-3, a novel transcription coactivator from Xenopus, that is related to AIB1, p/CIP and TIF2."
    Kim H.-J., Lee S.-K., Na S.-Y., Choi H.-S., Lee J.W.
    Mol. Endocrinol. 12:1038-1047(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH RXRA; THRA AND EP300, MUTAGENESIS OF LEU-622; LEU-683 AND LEU-739.
    Tissue: Oocyte.

Entry informationi

Entry nameiNCOA3_XENLA
AccessioniPrimary (citable) accession number: O57539
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: June 1, 1998
Last modified: June 11, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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