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O57539

- NCOA3_XENLA

UniProt

O57539 - NCOA3_XENLA

Protein

Nuclear receptor coactivator 3

Gene

ncoa3

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, probably via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as retinoids (RAR and RXR), thyroid hormone (TR) and orphan nuclear receptor (hepatocyte nuclear receptor 4 (HNF4) and constitutive androstane receptor (CAR)). Displays histone acetyltransferase activity.

    Catalytic activityi

    Acetyl-CoA + [histone] = CoA + acetyl-[histone].

    GO - Molecular functioni

    1. histone acetyltransferase activity Source: UniProtKB-EC
    2. ligand-dependent nuclear receptor transcription coactivator activity Source: InterPro
    3. protein binding Source: IntAct
    4. signal transducer activity Source: InterPro

    GO - Biological processi

    1. regulation of transcription, DNA-templated Source: UniProtKB-KW
    2. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Acyltransferase, Transferase

    Keywords - Biological processi

    Transcription, Transcription regulation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nuclear receptor coactivator 3 (EC:2.3.1.48)
    Alternative name(s):
    Retinoid X receptor-interacting coactivator xSRC-3
    Gene namesi
    Name:ncoa3
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-865628. ncoa3.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus PROSITE-ProRule annotation
    Note: Mainly cytoplasmic and weakly nuclear.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi622 – 6221L → A: Weakly impairs interaction with nuclear receptors. 1 Publication
    Mutagenesisi683 – 6831L → A: Strongly impairs interaction with nuclear receptors. 1 Publication
    Mutagenesisi739 – 7391L → A: Strongly impairs interaction with nuclear receptors. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13911391Nuclear receptor coactivator 3PRO_0000094409Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei614 – 6141N6-acetyllysineBy similarity
    Modified residuei617 – 6171N6-acetyllysineBy similarity
    Modified residuei618 – 6181N6-acetyllysineBy similarity

    Post-translational modificationi

    Phosphorylated and acetylated.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Expressioni

    Tissue specificityi

    Highly expressed in liver and in early stages of oocyte development.

    Developmental stagei

    Expressed only in early stages of oocyte development. Expression is more prominent in stage I, strongly decreases in stage II and then, gradually disappears.

    Interactioni

    Subunit structurei

    Interacts with the histone acetyltransferase protein EP300.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ncoa3O57539-12EBI-301587,EBI-301595

    Structurei

    3D structure databases

    ProteinModelPortaliO57539.
    SMRiO57539. Positions 1036-1082.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini27 – 8458bHLHPROSITE-ProRule annotationAdd
    BLAST
    Domaini112 – 18271PASPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1088 – 1274187AcetyltransferaseAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi680 – 6845LXXLL motif 1
    Motifi736 – 7405LXXLL motif 2
    Motifi1048 – 10525LXXLL motif 3

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi503 – 666164Ser-richAdd
    BLAST
    Compositional biasi515 – 5228Poly-Ser
    Compositional biasi968 – 9714Poly-Gln
    Compositional biasi1241 – 12488Poly-Gln

    Domaini

    Contains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain).

    Sequence similaritiesi

    Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation
    Contains 1 PAS (PER-ARNT-SIM) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    HOVERGENiHBG052583.
    KOiK11256.

    Family and domain databases

    Gene3Di4.10.280.10. 1 hit.
    4.10.630.10. 2 hits.
    InterProiIPR011598. bHLH_dom.
    IPR010011. DUF1518.
    IPR028818. NCOA3.
    IPR009110. Nuc_rcpt_coact.
    IPR014920. Nuc_rcpt_coact_Ncoa-typ.
    IPR017426. Nuclear_rcpt_coactivator.
    IPR000014. PAS.
    IPR013767. PAS_fold.
    IPR014935. SRC-1.
    IPR008955. Src1_rcpt_coact.
    [Graphical view]
    PANTHERiPTHR10684. PTHR10684. 1 hit.
    PTHR10684:SF3. PTHR10684:SF3. 1 hit.
    PfamiPF07469. DUF1518. 1 hit.
    PF08815. Nuc_rec_co-act. 1 hit.
    PF00989. PAS. 1 hit.
    PF08832. SRC-1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF038181. Nuclear_receptor_coactivator. 1 hit.
    SMARTiSM00353. HLH. 1 hit.
    SM00091. PAS. 1 hit.
    [Graphical view]
    SUPFAMiSSF47459. SSF47459. 1 hit.
    SSF55785. SSF55785. 2 hits.
    SSF69125. SSF69125. 1 hit.
    PROSITEiPS50888. BHLH. 1 hit.
    PS50112. PAS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms may be produced.

    Isoform 1 (identifier: O57539-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSGLGENSLD PLASETRKRK PSSCDTPGPG LTCSGEKRRR EQESKYIEEL     50
    ADLISANLSD IDNFNVKPDK CAILKETVRQ IRQIKEQGKA SSNDDDVQKA 100
    DVSSTGQGVI DKDSLGPLLL QALDGFLYVV NREGSIVFVS ENVTQYLQYK 150
    QEDLVNTSVY SILHEEDRKD FLKNLPKSTV NGVPWFSETP RQKSHTFNCR 200
    MLVKTSHDHL EDGSNLDARQ RYETMQCFAL SQPRAMIEEG EDLQSCMICV 250
    ARRITTAERA FSANPESFIT RHDLTGKVVN IDANSLRSSM RPGFEDTIRR 300
    CIQRFLFHSE GQPWTYKRHY QEAYVHGLSE TPLYRFSLAD GTMVTAQTKS 350
    KLFRNPVTND PHGFVSTHFL QREQNGYRPN PNPMAQGIRP QMNPNLPNTM 400
    NSMPPQAMQQ QNRNYGMGDP NSMAQMQGMR YKSPGNMAPV NQAPGVQQSP 450
    YQNNSNYGLN MNSPPHGSPG MNANQPNLMV SPRNRASPKM ASNQFSPVPG 500
    MNSPMGSSGN AGGGSFSSSS LSALHAISEG VGSSLLSSLS SPGQKVENNS 550
    NMNMPQQGKI CNQDCKSPSG LYCEQGQVES SVCQSSGREH LGEKDVKENI 600
    FEGSESQRSQ AESKGHKKLL QLLTCFTEER GQSLMSSSSM DCKDSSNVTS 650
    PSGVSSSTSI GVSSTSNLHG SMLQEKHRIL HKLLQNGNSP AEVAKITAEA 700
    TGKDVFQETV SSAPCTEATV KREQLSPKKK ENNALLRHLL DKDDWKDPLA 750
    KDIKPKVEHM DIKMGSCSSS NVPTSSQDKE VKIKTEPGEE VPGDLDNLDA 800
    ILGDLAGSDF YSNSMSSRAS DLGPKQPVFQ DSPTLAMRSP DSMQGSRPPF 850
    NRAMSLDSRS STPPVRNVNS FPMLPKQGMI GSPRMMDGQD NFGVMMGSGP 900
    NRSMNQHPGG DWAMQNSAVN RLEPPNVGSV GRPGPDYSSA MTRPAMGGNM 950
    PGLLTRSNSI PGSRPVMQQQ QHILPMRPND MAMSMGSNPY GQQAPSNPPG 1000
    SWPDAIMMNQ GRGGAQNRQL GRNSLDDLLC PPSTVEGQTD EIALLDQLHT 1050
    LLSNTDATGL EEIDRALGIP DLVSQGQALE PQPDSYQPQG SPVMIDQKPP 1100
    MYGQHYAGQG AAMSAGGFNN MQGQHPPFNT VMGQMNQQQG MHPLQGMHPR 1150
    ANLIRPRNNI PKQLRMQLQQ RLQGQQFLNQ NRQALEMKVD PMNPGGAGVM 1200
    RPVMQTPVSQ QGFLNAQMVA QKNRELISHQ IRQHRMAMMM QQQQGQPQAF 1250
    SPPPNVTASA SMDNPLGGPP MPQAPPQQFS YPPNYGINQQ TDPTFGRVSS 1300
    PPNAMMSSRM APSQNPHPQT TQMYPSPDMK GWPSGNMARP NSFPQQQYSH 1350
    QTNPATYNMM HMNGNGNHMG QMNINSLPMS GMPMGPDQKY C 1391
    Length:1,391
    Mass (Da):152,532
    Last modified:June 1, 1998 - v1
    Checksum:iAD28F5CD934AC33D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF044080 mRNA. Translation: AAC12927.1.
    RefSeqiNP_001081732.1. NM_001088263.1. [O57539-1]
    UniGeneiXl.268.

    Genome annotation databases

    GeneIDi398021.
    KEGGixla:398021.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF044080 mRNA. Translation: AAC12927.1 .
    RefSeqi NP_001081732.1. NM_001088263.1. [O57539-1 ]
    UniGenei Xl.268.

    3D structure databases

    ProteinModelPortali O57539.
    SMRi O57539. Positions 1036-1082.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 398021.
    KEGGi xla:398021.

    Organism-specific databases

    CTDi 8202.
    Xenbasei XB-GENE-865628. ncoa3.

    Phylogenomic databases

    HOVERGENi HBG052583.
    KOi K11256.

    Family and domain databases

    Gene3Di 4.10.280.10. 1 hit.
    4.10.630.10. 2 hits.
    InterProi IPR011598. bHLH_dom.
    IPR010011. DUF1518.
    IPR028818. NCOA3.
    IPR009110. Nuc_rcpt_coact.
    IPR014920. Nuc_rcpt_coact_Ncoa-typ.
    IPR017426. Nuclear_rcpt_coactivator.
    IPR000014. PAS.
    IPR013767. PAS_fold.
    IPR014935. SRC-1.
    IPR008955. Src1_rcpt_coact.
    [Graphical view ]
    PANTHERi PTHR10684. PTHR10684. 1 hit.
    PTHR10684:SF3. PTHR10684:SF3. 1 hit.
    Pfami PF07469. DUF1518. 1 hit.
    PF08815. Nuc_rec_co-act. 1 hit.
    PF00989. PAS. 1 hit.
    PF08832. SRC-1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF038181. Nuclear_receptor_coactivator. 1 hit.
    SMARTi SM00353. HLH. 1 hit.
    SM00091. PAS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47459. SSF47459. 1 hit.
    SSF55785. SSF55785. 2 hits.
    SSF69125. SSF69125. 1 hit.
    PROSITEi PS50888. BHLH. 1 hit.
    PS50112. PAS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of xSRC-3, a novel transcription coactivator from Xenopus, that is related to AIB1, p/CIP and TIF2."
      Kim H.-J., Lee S.-K., Na S.-Y., Choi H.-S., Lee J.W.
      Mol. Endocrinol. 12:1038-1047(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH RXRA; THRA AND EP300, MUTAGENESIS OF LEU-622; LEU-683 AND LEU-739.
      Tissue: Oocyte.

    Entry informationi

    Entry nameiNCOA3_XENLA
    AccessioniPrimary (citable) accession number: O57539
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 19, 2002
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3