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Protein

Nuclear receptor coactivator 3

Gene

ncoa3

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, probably via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as retinoids (RAR and RXR), thyroid hormone (TR) and orphan nuclear receptor (hepatocyte nuclear receptor 4 (HNF4) and constitutive androstane receptor (CAR)). Displays histone acetyltransferase activity.

Catalytic activityi

Acetyl-CoA + [histone] = CoA + acetyl-[histone].

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Acyltransferase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear receptor coactivator 3 (EC:2.3.1.48)
Alternative name(s):
Retinoid X receptor-interacting coactivator xSRC-3
Gene namesi
Name:ncoa3
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-865628. ncoa3.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus PROSITE-ProRule annotation

  • Note: Mainly cytoplasmic and weakly nuclear.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi622L → A: Weakly impairs interaction with nuclear receptors. 1 Publication1
Mutagenesisi683L → A: Strongly impairs interaction with nuclear receptors. 1 Publication1
Mutagenesisi739L → A: Strongly impairs interaction with nuclear receptors. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000944091 – 1391Nuclear receptor coactivator 3Add BLAST1391

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei614N6-acetyllysineBy similarity1
Modified residuei617N6-acetyllysineBy similarity1
Modified residuei618N6-acetyllysineBy similarity1

Post-translational modificationi

Phosphorylated and acetylated.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Expressioni

Tissue specificityi

Highly expressed in liver and in early stages of oocyte development.

Developmental stagei

Expressed only in early stages of oocyte development. Expression is more prominent in stage I, strongly decreases in stage II and then, gradually disappears.

Interactioni

Subunit structurei

Interacts with the histone acetyltransferase protein EP300.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ncoa3O57539-12EBI-301587,EBI-301595

Structurei

3D structure databases

ProteinModelPortaliO57539.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 84bHLHPROSITE-ProRule annotationAdd BLAST58
Domaini112 – 182PASPROSITE-ProRule annotationAdd BLAST71

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1088 – 1274AcetyltransferaseAdd BLAST187

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi680 – 684LXXLL motif 15
Motifi736 – 740LXXLL motif 25
Motifi1048 – 1052LXXLL motif 35

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi503 – 666Ser-richAdd BLAST164
Compositional biasi515 – 522Poly-Ser8
Compositional biasi968 – 971Poly-Gln4
Compositional biasi1241 – 1248Poly-Gln8

Domaini

Contains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain).

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation
Contains 1 PAS (PER-ARNT-SIM) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOVERGENiHBG052583.
KOiK11256.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
4.10.630.10. 2 hits.
InterProiIPR011598. bHLH_dom.
IPR010011. DUF1518.
IPR032565. DUF4927.
IPR028818. NCOA3.
IPR009110. Nuc_rcpt_coact.
IPR014920. Nuc_rcpt_coact_Ncoa-typ.
IPR017426. Nuclear_rcpt_coactivator.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR014935. SRC-1.
IPR008955. Src1_rcpt_coact.
[Graphical view]
PANTHERiPTHR10684. PTHR10684. 1 hit.
PTHR10684:SF3. PTHR10684:SF3. 1 hit.
PfamiPF07469. DUF1518. 1 hit.
PF16279. DUF4927. 1 hit.
PF08815. Nuc_rec_co-act. 1 hit.
PF00989. PAS. 1 hit.
PF08832. SRC-1. 1 hit.
[Graphical view]
PIRSFiPIRSF038181. Nuclear_receptor_coactivator. 1 hit.
SMARTiSM01151. DUF1518. 1 hit.
SM00353. HLH. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
SSF55785. SSF55785. 2 hits.
SSF69125. SSF69125. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
PS50112. PAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms may be produced.
Isoform 1 (identifier: O57539-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGLGENSLD PLASETRKRK PSSCDTPGPG LTCSGEKRRR EQESKYIEEL
60 70 80 90 100
ADLISANLSD IDNFNVKPDK CAILKETVRQ IRQIKEQGKA SSNDDDVQKA
110 120 130 140 150
DVSSTGQGVI DKDSLGPLLL QALDGFLYVV NREGSIVFVS ENVTQYLQYK
160 170 180 190 200
QEDLVNTSVY SILHEEDRKD FLKNLPKSTV NGVPWFSETP RQKSHTFNCR
210 220 230 240 250
MLVKTSHDHL EDGSNLDARQ RYETMQCFAL SQPRAMIEEG EDLQSCMICV
260 270 280 290 300
ARRITTAERA FSANPESFIT RHDLTGKVVN IDANSLRSSM RPGFEDTIRR
310 320 330 340 350
CIQRFLFHSE GQPWTYKRHY QEAYVHGLSE TPLYRFSLAD GTMVTAQTKS
360 370 380 390 400
KLFRNPVTND PHGFVSTHFL QREQNGYRPN PNPMAQGIRP QMNPNLPNTM
410 420 430 440 450
NSMPPQAMQQ QNRNYGMGDP NSMAQMQGMR YKSPGNMAPV NQAPGVQQSP
460 470 480 490 500
YQNNSNYGLN MNSPPHGSPG MNANQPNLMV SPRNRASPKM ASNQFSPVPG
510 520 530 540 550
MNSPMGSSGN AGGGSFSSSS LSALHAISEG VGSSLLSSLS SPGQKVENNS
560 570 580 590 600
NMNMPQQGKI CNQDCKSPSG LYCEQGQVES SVCQSSGREH LGEKDVKENI
610 620 630 640 650
FEGSESQRSQ AESKGHKKLL QLLTCFTEER GQSLMSSSSM DCKDSSNVTS
660 670 680 690 700
PSGVSSSTSI GVSSTSNLHG SMLQEKHRIL HKLLQNGNSP AEVAKITAEA
710 720 730 740 750
TGKDVFQETV SSAPCTEATV KREQLSPKKK ENNALLRHLL DKDDWKDPLA
760 770 780 790 800
KDIKPKVEHM DIKMGSCSSS NVPTSSQDKE VKIKTEPGEE VPGDLDNLDA
810 820 830 840 850
ILGDLAGSDF YSNSMSSRAS DLGPKQPVFQ DSPTLAMRSP DSMQGSRPPF
860 870 880 890 900
NRAMSLDSRS STPPVRNVNS FPMLPKQGMI GSPRMMDGQD NFGVMMGSGP
910 920 930 940 950
NRSMNQHPGG DWAMQNSAVN RLEPPNVGSV GRPGPDYSSA MTRPAMGGNM
960 970 980 990 1000
PGLLTRSNSI PGSRPVMQQQ QHILPMRPND MAMSMGSNPY GQQAPSNPPG
1010 1020 1030 1040 1050
SWPDAIMMNQ GRGGAQNRQL GRNSLDDLLC PPSTVEGQTD EIALLDQLHT
1060 1070 1080 1090 1100
LLSNTDATGL EEIDRALGIP DLVSQGQALE PQPDSYQPQG SPVMIDQKPP
1110 1120 1130 1140 1150
MYGQHYAGQG AAMSAGGFNN MQGQHPPFNT VMGQMNQQQG MHPLQGMHPR
1160 1170 1180 1190 1200
ANLIRPRNNI PKQLRMQLQQ RLQGQQFLNQ NRQALEMKVD PMNPGGAGVM
1210 1220 1230 1240 1250
RPVMQTPVSQ QGFLNAQMVA QKNRELISHQ IRQHRMAMMM QQQQGQPQAF
1260 1270 1280 1290 1300
SPPPNVTASA SMDNPLGGPP MPQAPPQQFS YPPNYGINQQ TDPTFGRVSS
1310 1320 1330 1340 1350
PPNAMMSSRM APSQNPHPQT TQMYPSPDMK GWPSGNMARP NSFPQQQYSH
1360 1370 1380 1390
QTNPATYNMM HMNGNGNHMG QMNINSLPMS GMPMGPDQKY C
Length:1,391
Mass (Da):152,532
Last modified:June 1, 1998 - v1
Checksum:iAD28F5CD934AC33D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044080 mRNA. Translation: AAC12927.1.
RefSeqiNP_001081732.1. NM_001088263.1. [O57539-1]
UniGeneiXl.268.

Genome annotation databases

GeneIDi398021.
KEGGixla:398021.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044080 mRNA. Translation: AAC12927.1.
RefSeqiNP_001081732.1. NM_001088263.1. [O57539-1]
UniGeneiXl.268.

3D structure databases

ProteinModelPortaliO57539.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi398021.
KEGGixla:398021.

Organism-specific databases

CTDi8202.
XenbaseiXB-GENE-865628. ncoa3.

Phylogenomic databases

HOVERGENiHBG052583.
KOiK11256.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
4.10.630.10. 2 hits.
InterProiIPR011598. bHLH_dom.
IPR010011. DUF1518.
IPR032565. DUF4927.
IPR028818. NCOA3.
IPR009110. Nuc_rcpt_coact.
IPR014920. Nuc_rcpt_coact_Ncoa-typ.
IPR017426. Nuclear_rcpt_coactivator.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR014935. SRC-1.
IPR008955. Src1_rcpt_coact.
[Graphical view]
PANTHERiPTHR10684. PTHR10684. 1 hit.
PTHR10684:SF3. PTHR10684:SF3. 1 hit.
PfamiPF07469. DUF1518. 1 hit.
PF16279. DUF4927. 1 hit.
PF08815. Nuc_rec_co-act. 1 hit.
PF00989. PAS. 1 hit.
PF08832. SRC-1. 1 hit.
[Graphical view]
PIRSFiPIRSF038181. Nuclear_receptor_coactivator. 1 hit.
SMARTiSM01151. DUF1518. 1 hit.
SM00353. HLH. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
SSF55785. SSF55785. 2 hits.
SSF69125. SSF69125. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
PS50112. PAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNCOA3_XENLA
AccessioniPrimary (citable) accession number: O57539
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: June 1, 1998
Last modified: October 5, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.