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O57539

- NCOA3_XENLA

UniProt

O57539 - NCOA3_XENLA

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Protein

Nuclear receptor coactivator 3

Gene

ncoa3

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, probably via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as retinoids (RAR and RXR), thyroid hormone (TR) and orphan nuclear receptor (hepatocyte nuclear receptor 4 (HNF4) and constitutive androstane receptor (CAR)). Displays histone acetyltransferase activity.

Catalytic activityi

Acetyl-CoA + [histone] = CoA + acetyl-[histone].

GO - Molecular functioni

  1. histone acetyltransferase activity Source: UniProtKB-EC
  2. ligand-dependent nuclear receptor transcription coactivator activity Source: InterPro
  3. signal transducer activity Source: InterPro

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: UniProtKB-KW
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Acyltransferase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear receptor coactivator 3 (EC:2.3.1.48)
Alternative name(s):
Retinoid X receptor-interacting coactivator xSRC-3
Gene namesi
Name:ncoa3
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-865628. ncoa3.

Subcellular locationi

Cytoplasm By similarity. Nucleus PROSITE-ProRule annotation
Note: Mainly cytoplasmic and weakly nuclear.By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi622 – 6221L → A: Weakly impairs interaction with nuclear receptors. 1 Publication
Mutagenesisi683 – 6831L → A: Strongly impairs interaction with nuclear receptors. 1 Publication
Mutagenesisi739 – 7391L → A: Strongly impairs interaction with nuclear receptors. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13911391Nuclear receptor coactivator 3PRO_0000094409Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei614 – 6141N6-acetyllysineBy similarity
Modified residuei617 – 6171N6-acetyllysineBy similarity
Modified residuei618 – 6181N6-acetyllysineBy similarity

Post-translational modificationi

Phosphorylated and acetylated.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Expressioni

Tissue specificityi

Highly expressed in liver and in early stages of oocyte development.

Developmental stagei

Expressed only in early stages of oocyte development. Expression is more prominent in stage I, strongly decreases in stage II and then, gradually disappears.

Interactioni

Subunit structurei

Interacts with the histone acetyltransferase protein EP300.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ncoa3O57539-12EBI-301587,EBI-301595

Structurei

3D structure databases

ProteinModelPortaliO57539.
SMRiO57539. Positions 1036-1082.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 8458bHLHPROSITE-ProRule annotationAdd
BLAST
Domaini112 – 18271PASPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1088 – 1274187AcetyltransferaseAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi680 – 6845LXXLL motif 1
Motifi736 – 7405LXXLL motif 2
Motifi1048 – 10525LXXLL motif 3

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi503 – 666164Ser-richAdd
BLAST
Compositional biasi515 – 5228Poly-Ser
Compositional biasi968 – 9714Poly-Gln
Compositional biasi1241 – 12488Poly-Gln

Domaini

Contains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain).

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation
Contains 1 PAS (PER-ARNT-SIM) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOVERGENiHBG052583.
KOiK11256.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
4.10.630.10. 2 hits.
InterProiIPR011598. bHLH_dom.
IPR010011. DUF1518.
IPR028818. NCOA3.
IPR009110. Nuc_rcpt_coact.
IPR014920. Nuc_rcpt_coact_Ncoa-typ.
IPR017426. Nuclear_rcpt_coactivator.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR014935. SRC-1.
IPR008955. Src1_rcpt_coact.
[Graphical view]
PANTHERiPTHR10684. PTHR10684. 1 hit.
PTHR10684:SF3. PTHR10684:SF3. 1 hit.
PfamiPF07469. DUF1518. 1 hit.
PF08815. Nuc_rec_co-act. 1 hit.
PF00989. PAS. 1 hit.
PF08832. SRC-1. 1 hit.
[Graphical view]
PIRSFiPIRSF038181. Nuclear_receptor_coactivator. 1 hit.
SMARTiSM00353. HLH. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
SSF55785. SSF55785. 2 hits.
SSF69125. SSF69125. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
PS50112. PAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms may be produced.

Isoform 1 (identifier: O57539-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGLGENSLD PLASETRKRK PSSCDTPGPG LTCSGEKRRR EQESKYIEEL
60 70 80 90 100
ADLISANLSD IDNFNVKPDK CAILKETVRQ IRQIKEQGKA SSNDDDVQKA
110 120 130 140 150
DVSSTGQGVI DKDSLGPLLL QALDGFLYVV NREGSIVFVS ENVTQYLQYK
160 170 180 190 200
QEDLVNTSVY SILHEEDRKD FLKNLPKSTV NGVPWFSETP RQKSHTFNCR
210 220 230 240 250
MLVKTSHDHL EDGSNLDARQ RYETMQCFAL SQPRAMIEEG EDLQSCMICV
260 270 280 290 300
ARRITTAERA FSANPESFIT RHDLTGKVVN IDANSLRSSM RPGFEDTIRR
310 320 330 340 350
CIQRFLFHSE GQPWTYKRHY QEAYVHGLSE TPLYRFSLAD GTMVTAQTKS
360 370 380 390 400
KLFRNPVTND PHGFVSTHFL QREQNGYRPN PNPMAQGIRP QMNPNLPNTM
410 420 430 440 450
NSMPPQAMQQ QNRNYGMGDP NSMAQMQGMR YKSPGNMAPV NQAPGVQQSP
460 470 480 490 500
YQNNSNYGLN MNSPPHGSPG MNANQPNLMV SPRNRASPKM ASNQFSPVPG
510 520 530 540 550
MNSPMGSSGN AGGGSFSSSS LSALHAISEG VGSSLLSSLS SPGQKVENNS
560 570 580 590 600
NMNMPQQGKI CNQDCKSPSG LYCEQGQVES SVCQSSGREH LGEKDVKENI
610 620 630 640 650
FEGSESQRSQ AESKGHKKLL QLLTCFTEER GQSLMSSSSM DCKDSSNVTS
660 670 680 690 700
PSGVSSSTSI GVSSTSNLHG SMLQEKHRIL HKLLQNGNSP AEVAKITAEA
710 720 730 740 750
TGKDVFQETV SSAPCTEATV KREQLSPKKK ENNALLRHLL DKDDWKDPLA
760 770 780 790 800
KDIKPKVEHM DIKMGSCSSS NVPTSSQDKE VKIKTEPGEE VPGDLDNLDA
810 820 830 840 850
ILGDLAGSDF YSNSMSSRAS DLGPKQPVFQ DSPTLAMRSP DSMQGSRPPF
860 870 880 890 900
NRAMSLDSRS STPPVRNVNS FPMLPKQGMI GSPRMMDGQD NFGVMMGSGP
910 920 930 940 950
NRSMNQHPGG DWAMQNSAVN RLEPPNVGSV GRPGPDYSSA MTRPAMGGNM
960 970 980 990 1000
PGLLTRSNSI PGSRPVMQQQ QHILPMRPND MAMSMGSNPY GQQAPSNPPG
1010 1020 1030 1040 1050
SWPDAIMMNQ GRGGAQNRQL GRNSLDDLLC PPSTVEGQTD EIALLDQLHT
1060 1070 1080 1090 1100
LLSNTDATGL EEIDRALGIP DLVSQGQALE PQPDSYQPQG SPVMIDQKPP
1110 1120 1130 1140 1150
MYGQHYAGQG AAMSAGGFNN MQGQHPPFNT VMGQMNQQQG MHPLQGMHPR
1160 1170 1180 1190 1200
ANLIRPRNNI PKQLRMQLQQ RLQGQQFLNQ NRQALEMKVD PMNPGGAGVM
1210 1220 1230 1240 1250
RPVMQTPVSQ QGFLNAQMVA QKNRELISHQ IRQHRMAMMM QQQQGQPQAF
1260 1270 1280 1290 1300
SPPPNVTASA SMDNPLGGPP MPQAPPQQFS YPPNYGINQQ TDPTFGRVSS
1310 1320 1330 1340 1350
PPNAMMSSRM APSQNPHPQT TQMYPSPDMK GWPSGNMARP NSFPQQQYSH
1360 1370 1380 1390
QTNPATYNMM HMNGNGNHMG QMNINSLPMS GMPMGPDQKY C
Length:1,391
Mass (Da):152,532
Last modified:June 1, 1998 - v1
Checksum:iAD28F5CD934AC33D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF044080 mRNA. Translation: AAC12927.1.
RefSeqiNP_001081732.1. NM_001088263.1. [O57539-1]
UniGeneiXl.268.

Genome annotation databases

GeneIDi398021.
KEGGixla:398021.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF044080 mRNA. Translation: AAC12927.1 .
RefSeqi NP_001081732.1. NM_001088263.1. [O57539-1 ]
UniGenei Xl.268.

3D structure databases

ProteinModelPortali O57539.
SMRi O57539. Positions 1036-1082.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 398021.
KEGGi xla:398021.

Organism-specific databases

CTDi 8202.
Xenbasei XB-GENE-865628. ncoa3.

Phylogenomic databases

HOVERGENi HBG052583.
KOi K11256.

Family and domain databases

Gene3Di 4.10.280.10. 1 hit.
4.10.630.10. 2 hits.
InterProi IPR011598. bHLH_dom.
IPR010011. DUF1518.
IPR028818. NCOA3.
IPR009110. Nuc_rcpt_coact.
IPR014920. Nuc_rcpt_coact_Ncoa-typ.
IPR017426. Nuclear_rcpt_coactivator.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR014935. SRC-1.
IPR008955. Src1_rcpt_coact.
[Graphical view ]
PANTHERi PTHR10684. PTHR10684. 1 hit.
PTHR10684:SF3. PTHR10684:SF3. 1 hit.
Pfami PF07469. DUF1518. 1 hit.
PF08815. Nuc_rec_co-act. 1 hit.
PF00989. PAS. 1 hit.
PF08832. SRC-1. 1 hit.
[Graphical view ]
PIRSFi PIRSF038181. Nuclear_receptor_coactivator. 1 hit.
SMARTi SM00353. HLH. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view ]
SUPFAMi SSF47459. SSF47459. 1 hit.
SSF55785. SSF55785. 2 hits.
SSF69125. SSF69125. 1 hit.
PROSITEi PS50888. BHLH. 1 hit.
PS50112. PAS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of xSRC-3, a novel transcription coactivator from Xenopus, that is related to AIB1, p/CIP and TIF2."
    Kim H.-J., Lee S.-K., Na S.-Y., Choi H.-S., Lee J.W.
    Mol. Endocrinol. 12:1038-1047(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH RXRA; THRA AND EP300, MUTAGENESIS OF LEU-622; LEU-683 AND LEU-739.
    Tissue: Oocyte.

Entry informationi

Entry nameiNCOA3_XENLA
AccessioniPrimary (citable) accession number: O57539
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: June 1, 1998
Last modified: October 29, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3