O57525 (CP17A_RANDY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Steroid 17-alpha-hydroxylase/17,20 lyase
EC=1.14.99.9
EC=4.1.2.30

Alternative name(s):
17-alpha-hydroxyprogesterone aldolase
CYPXVII
Cytochrome P450 17A1
Cytochrome P450-C17
Short name=Cytochrome P450c17

Gene names

Name:	CYP17A1
Synonyms:	CYP17

Organism

Rana dybowskii (Dybovsky's frog) (Korean brown frog)

Taxonomic identifier

71582 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Neobatrachia › Ranoidea › Ranidae › Rana › Rana

Protein attributes

Sequence length	519 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction.
Catalytic activity	A C(21)-steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 17-alpha-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O. 17-alpha-hydroxyprogesterone = androst-4-ene-3,17-dione + acetaldehyde.
Cofactor	Heme group By similarity.
Pathway	Lipid metabolism; steroid biosynthesis.
Subcellular location	Membrane Potential.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Steroidogenesis
Cellular component	Membrane
Ligand	Heme Iron Metal-binding
Molecular function	Lyase Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological_process	steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	17-alpha-hydroxyprogesterone aldolase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro steroid 17-alpha-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 519	519	Steroid 17-alpha-hydroxylase/17,20 lyase		PRO_0000051948
Sites
	Metal binding	455	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	O57525 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: FDF866CF15593A2E	FASTA	519	58,948
	10 20 30 40 50 60 MKLCFFLLIF IRSFLLFKIK LVRTSEKKWR MGSRSHGDVK HPKSLPSLPV IGSLLHLGKK 70 80 90 100 110 120 LPPHILFCNL QKKYGSLYSF MMGSHYVVVV NNHEDAREVL LKKGKTFGGR PRTVTTDILT 130 140 150 160 170 180 RDGKDIAFAD YSPTWKFHRK MVHSALCMFG EGTVAIEQII SREAASLCQT LTSFQRIPLD 190 200 210 220 230 240 MAPELIRAVT NVVCSLCFNT RYKRGDAEFE TMLKYSKGIV DTVAKDSLVD IFPWLQIFPN 250 260 270 280 290 300 KDLDILRQSV AARDQLLQKK INEHKDAFCG ETVKDLVDAL LKAKLNMENN NSNVSQDVGL 310 320 330 340 350 360 TEDHILMTVG DIFGAGVETT STVLKWAVAY LLHYPEVQKK IQEELDVKVG FGRYPLLSDR 370 380 390 400 410 420 KILHYTEAAI SEVLRIRPVS PLLIPHVALK ESSIGEYTIP KEARVVINLW SLHHDEKEWV 430 440 450 460 470 480 NPHLFSPDRF LDENGNRVYS PSPSFLPFGA GIRVCLGEAL AKMEVFLFLS WILQRFTLEV 490 500 510 PEGDPLPDLE GKFGVVIQVK PFKVIAKLRE VWKNIEIVT

References

[1]	"Cloning and characterization of cDNA encoding 17-alpha-hydroxylase/17,20 lyase (P450c17) in amphibia (Rana dybowskii)." Choi H.H., Kang H.M., Choi H.S., Kwon H.B. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF042278 mRNA. Translation: AAB97686.1.
3D structure databases
ProteinModelPortal	O57525.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG106944.
Enzyme and pathway databases
UniPathway	UPA00062.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP17A_RANDY

Accession

Primary (citable) accession number: O57525

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	May 29, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program