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Protein

Steroid 17-alpha-hydroxylase/17,20 lyase

Gene

CYP17A1

Organism
Rana dybowskii (Dybovsky's frog) (Korean brown frog)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction.

Catalytic activityi

A C(21)-steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 17-alpha-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O.
17-alpha-hydroxyprogesterone = androst-4-ene-3,17-dione + acetaldehyde.

Cofactori

hemeBy similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi455 – 4551Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. 17-alpha-hydroxyprogesterone aldolase activity Source: UniProtKB-EC
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro
  4. steroid 17-alpha-monooxygenase activity Source: UniProtKB-EC

GO - Biological processi

  1. steroid biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Monooxygenase, Oxidoreductase

Keywords - Biological processi

Steroidogenesis

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00062.

Names & Taxonomyi

Protein namesi
Recommended name:
Steroid 17-alpha-hydroxylase/17,20 lyase (EC:1.14.99.9, EC:4.1.2.30)
Alternative name(s):
17-alpha-hydroxyprogesterone aldolase
CYPXVII
Cytochrome P450 17A1
Cytochrome P450-C17
Short name:
Cytochrome P450c17
Gene namesi
Name:CYP17A1
Synonyms:CYP17
OrganismiRana dybowskii (Dybovsky's frog) (Korean brown frog)
Taxonomic identifieri71582 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraNeobatrachiaRanoideaRanidaeRanaRana

Subcellular locationi

  1. Membrane Curated

GO - Cellular componenti

  1. membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 519519Steroid 17-alpha-hydroxylase/17,20 lyasePRO_0000051948Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliO57525.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

HOVERGENiHBG106944.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O57525-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLCFFLLIF IRSFLLFKIK LVRTSEKKWR MGSRSHGDVK HPKSLPSLPV
60 70 80 90 100
IGSLLHLGKK LPPHILFCNL QKKYGSLYSF MMGSHYVVVV NNHEDAREVL
110 120 130 140 150
LKKGKTFGGR PRTVTTDILT RDGKDIAFAD YSPTWKFHRK MVHSALCMFG
160 170 180 190 200
EGTVAIEQII SREAASLCQT LTSFQRIPLD MAPELIRAVT NVVCSLCFNT
210 220 230 240 250
RYKRGDAEFE TMLKYSKGIV DTVAKDSLVD IFPWLQIFPN KDLDILRQSV
260 270 280 290 300
AARDQLLQKK INEHKDAFCG ETVKDLVDAL LKAKLNMENN NSNVSQDVGL
310 320 330 340 350
TEDHILMTVG DIFGAGVETT STVLKWAVAY LLHYPEVQKK IQEELDVKVG
360 370 380 390 400
FGRYPLLSDR KILHYTEAAI SEVLRIRPVS PLLIPHVALK ESSIGEYTIP
410 420 430 440 450
KEARVVINLW SLHHDEKEWV NPHLFSPDRF LDENGNRVYS PSPSFLPFGA
460 470 480 490 500
GIRVCLGEAL AKMEVFLFLS WILQRFTLEV PEGDPLPDLE GKFGVVIQVK
510
PFKVIAKLRE VWKNIEIVT
Length:519
Mass (Da):58,948
Last modified:June 1, 1998 - v1
Checksum:iFDF866CF15593A2E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF042278 mRNA. Translation: AAB97686.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF042278 mRNA. Translation: AAB97686.1.

3D structure databases

ProteinModelPortaliO57525.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG106944.

Enzyme and pathway databases

UniPathwayiUPA00062.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and characterization of cDNA encoding 17-alpha-hydroxylase/17,20 lyase (P450c17) in amphibia (Rana dybowskii)."
    Choi H.H., Kang H.M., Choi H.S., Kwon H.B.
    Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiCP17A_RANDY
AccessioniPrimary (citable) accession number: O57525
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 1, 1998
Last modified: January 7, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.