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O57473

- WEE2B_XENLA

UniProt

O57473 - WEE2B_XENLA

Protein

Wee1-like protein kinase 2-B

Gene

wee2-b

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Oocyte and early embryo-specific protein tyrosine kinase that phosphorylates and inhibits cdk1 and acts as a regulator of meiosis in oocytes. Required to ensure the meiotic cell cycle in oocytes by phosphorylating cdk1 at 'Tyr-15', leading to inhibit cdk1 activity and prevent meiosis.1 Publication

    Catalytic activityi

    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.1 PublicationPROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei242 – 2421ATPPROSITE-ProRule annotation
    Active sitei340 – 3401Proton acceptorPROSITE-ProRule annotation
    Metal bindingi345 – 3451Magnesium; via carbonyl oxygenBy similarity
    Metal bindingi377 – 3771Magnesium; via carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi219 – 2279ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
    4. protein serine/threonine kinase activity Source: InterPro

    GO - Biological processi

    1. meiotic nuclear division Source: UniProtKB-KW
    2. mitotic nuclear division Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase, Tyrosine-protein kinase

    Keywords - Biological processi

    Meiosis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Wee1-like protein kinase 2-B (EC:2.7.10.2)
    Alternative name(s):
    Wee1-like protein kinase 1
    Xe-wee1
    Gene namesi
    Name:wee2-b
    Synonyms:wee1
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi38 – 381S → A: Strongly reduces the interaction with cdca3. 1 Publication
    Mutagenesisi242 – 2421K → M: Dominant-negative mutant; reduces the level of cdk1 tyrosine phosphorylation and shortened the length of the cell cycle. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 554554Wee1-like protein kinase 2-BPRO_0000409529Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei38 – 381Phosphoserine1 Publication

    Post-translational modificationi

    Ubiquitinated and degraded at the onset of G2/M phase.1 Publication
    Phosphorylated during M and G1 phases. Interacts with cdca3 when phosphorylated at Ser-38.1 Publication

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Expressioni

    Developmental stagei

    Absent in stage VI oocytes and is expressed from meiosis II until gastrulation.1 Publication

    Interactioni

    Subunit structurei

    Interacts with cdca3.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliO57473.
    SMRiO57473. Positions 205-483.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini213 – 487275Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili490 – 51627Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    HOVERGENiHBG005050.
    KOiK06632.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR008271. Ser/Thr_kinase_AS.
    IPR017164. Wee1-like_protein_kinase.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037281. Wee1-like_protein_kinase. 1 hit.
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O57473-1 [UniParc]FASTAAdd to Basket

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    MRMAMSCGGR LVQRLDFSSS EEEDGLSNRI NEAPQKGSPV SSWRTNNCPF    50
    PITPQRNERG LSPTQELSPS SDYSPDPSDK GVGGECPGTP LHYSTWKKLK 100
    LCDTPYTPKS LLYKTLPSPG SRVHCRGQRL LRFVAGTGAE TEDPTLVNVN 150
    PFTPQSYRQT HFQPNGKRKE RPEDDCSSDS QMKFTDKEHP AVFQSKRFVL 200
    RETNMESRYK TEFLEIEKIG AGEFGSVFKC VKRLDGCFYV IKRSKKPLAG 250
    STDEQLALRE VYAHAVLGHH PHVVRYYSAW AEDDHMIIQN EYCNGGSLQD 300
    LIMENNKKGQ FVPEQELKEI LLQVSMGLKY IHGSGLVHMD IKPSNIFICR 350
    KQTEVGEDES DGEDDVASAS VLYKIGDLGH VTSILNPQVE EGDSRFLANE 400
    ILQEDYRQLP KADIFALGLT IALAAGAAPL PCNEDGWHHI RKGNLPHIPQ 450
    PLTPAFLALL KLLVHPDPAT RPPATSLAKN SVLRRCVGKA AELQKQLNVE 500
    KFKTAMLERE LQAAKLAQDE CLDLPPVSGF SCRGRKRLVG AKNARSLSFT 550
    CGGY 554
    Length:554
    Mass (Da):61,666
    Last modified:June 1, 1998 - v1
    Checksum:i30021FB7A95C5F0D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti190 – 1901P → S in AAI69848. 1 PublicationCurated
    Sequence conflicti190 – 1901P → S in AAI69846. 1 PublicationCurated
    Sequence conflicti367 – 3671A → S in AAI69848. 1 PublicationCurated
    Sequence conflicti367 – 3671A → S in AAI69846. 1 PublicationCurated
    Sequence conflicti469 – 4691A → V in AAI69848. 1 PublicationCurated
    Sequence conflicti469 – 4691A → V in AAI69846. 1 PublicationCurated
    Sequence conflicti475 – 4751T → A in AAI69848. 1 PublicationCurated
    Sequence conflicti475 – 4751T → A in AAI69846. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF035443 mRNA. Translation: AAB99952.1.
    BC169846 mRNA. Translation: AAI69846.1.
    BC169848 mRNA. Translation: AAI69848.1.
    RefSeqiNP_001081724.1. NM_001088255.1.
    UniGeneiXl.4222.

    Genome annotation databases

    GeneIDi398017.
    KEGGixla:398017.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF035443 mRNA. Translation: AAB99952.1 .
    BC169846 mRNA. Translation: AAI69846.1 .
    BC169848 mRNA. Translation: AAI69848.1 .
    RefSeqi NP_001081724.1. NM_001088255.1.
    UniGenei Xl.4222.

    3D structure databases

    ProteinModelPortali O57473.
    SMRi O57473. Positions 205-483.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 398017.
    KEGGi xla:398017.

    Organism-specific databases

    CTDi 494551.

    Phylogenomic databases

    HOVERGENi HBG005050.
    KOi K06632.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR008271. Ser/Thr_kinase_AS.
    IPR017164. Wee1-like_protein_kinase.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037281. Wee1-like_protein_kinase. 1 hit.
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of the early embryonic cell cycles of Xenopus; regulation of cell cycle length by Xe-wee1 and Mos."
      Murakami M.S., Vande Woude G.F.
      Development 125:237-248(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, MUTAGENESIS OF LYS-242.
    2. NIH - Xenopus Gene Collection (XGC) project
      Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Gastrula.
    3. "Tome-1, a trigger of mitotic entry, is degraded during G1 via the APC."
      Ayad N.G., Rankin S., Murakami M., Jebanathirajah J., Gygi S.P., Kirschner M.W.
      Cell 113:101-113(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT SER-38, UBIQUITINATION, INTERACTION WITH CDCA3, MUTAGENESIS OF SER-38.

    Entry informationi

    Entry nameiWEE2B_XENLA
    AccessioniPrimary (citable) accession number: O57473
    Secondary accession number(s): B7ZQL0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 31, 2011
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Caution

    Was initially assigned as wee1 (PubMed:9486797). However, it corresponds to the meiosis-specific protein WEE2 in mammals.1 Publication

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3