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O57473 (WEE2B_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Wee1-like protein kinase 2-B

EC=2.7.10.2
Alternative name(s):
Wee1-like protein kinase 1
Xe-wee1
Gene names
Name:wee2-b
Synonyms:wee1
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Protein attributes

Sequence length554 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Oocyte and early embryo-specific protein tyrosine kinase that phosphorylates and inhibits cdk1 and acts as a regulator of meiosis in oocytes. Required to ensure the meiotic cell cycle in oocytes by phosphorylating cdk1 at 'Tyr-15', leading to inhibit cdk1 activity and prevent meiosis. Ref.1

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. Ref.1

Subunit structure

Interacts with cdca3. Ref.3

Subcellular location

Nucleus By similarity.

Developmental stage

Absent in stage VI oocytes and is expressed from meiosis II until gastrulation. Ref.1

Post-translational modification

Ubiquitinated and degraded at the onset of G2/M phase. Ref.3

Phosphorylated during M and G1 phases. Interacts with cdca3 when phosphorylated at Ser-38. Ref.3

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.

Contains 1 protein kinase domain.

Caution

Was initially assigned as wee1 (Ref.1). However, it corresponds to the meiosis-specific protein WEE2 in mammals.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 554554Wee1-like protein kinase 2-B
PRO_0000409529

Regions

Domain213 – 487275Protein kinase
Nucleotide binding219 – 2279ATP By similarity
Coiled coil490 – 51627 Potential

Sites

Active site3401Proton acceptor By similarity
Metal binding3451Magnesium; via carbonyl oxygen By similarity
Metal binding3771Magnesium; via carbonyl oxygen By similarity
Binding site2421ATP By similarity

Amino acid modifications

Modified residue381Phosphoserine Ref.3

Experimental info

Mutagenesis381S → A: Strongly reduces the interaction with cdca3. Ref.3
Mutagenesis2421K → M: Dominant-negative mutant; reduces the level of cdk1 tyrosine phosphorylation and shortened the length of the cell cycle. Ref.1
Sequence conflict1901P → S in AAI69848. Ref.2
Sequence conflict1901P → S in AAI69846. Ref.2
Sequence conflict3671A → S in AAI69848. Ref.2
Sequence conflict3671A → S in AAI69846. Ref.2
Sequence conflict4691A → V in AAI69848. Ref.2
Sequence conflict4691A → V in AAI69846. Ref.2
Sequence conflict4751T → A in AAI69848. Ref.2
Sequence conflict4751T → A in AAI69846. Ref.2

Sequences

Sequence LengthMass (Da)Tools
O57473 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 30021FB7A95C5F0D

FASTA55461,666
        10         20         30         40         50         60 
MRMAMSCGGR LVQRLDFSSS EEEDGLSNRI NEAPQKGSPV SSWRTNNCPF PITPQRNERG 

        70         80         90        100        110        120 
LSPTQELSPS SDYSPDPSDK GVGGECPGTP LHYSTWKKLK LCDTPYTPKS LLYKTLPSPG 

       130        140        150        160        170        180 
SRVHCRGQRL LRFVAGTGAE TEDPTLVNVN PFTPQSYRQT HFQPNGKRKE RPEDDCSSDS 

       190        200        210        220        230        240 
QMKFTDKEHP AVFQSKRFVL RETNMESRYK TEFLEIEKIG AGEFGSVFKC VKRLDGCFYV 

       250        260        270        280        290        300 
IKRSKKPLAG STDEQLALRE VYAHAVLGHH PHVVRYYSAW AEDDHMIIQN EYCNGGSLQD 

       310        320        330        340        350        360 
LIMENNKKGQ FVPEQELKEI LLQVSMGLKY IHGSGLVHMD IKPSNIFICR KQTEVGEDES 

       370        380        390        400        410        420 
DGEDDVASAS VLYKIGDLGH VTSILNPQVE EGDSRFLANE ILQEDYRQLP KADIFALGLT 

       430        440        450        460        470        480 
IALAAGAAPL PCNEDGWHHI RKGNLPHIPQ PLTPAFLALL KLLVHPDPAT RPPATSLAKN 

       490        500        510        520        530        540 
SVLRRCVGKA AELQKQLNVE KFKTAMLERE LQAAKLAQDE CLDLPPVSGF SCRGRKRLVG 

       550 
AKNARSLSFT CGGY 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of the early embryonic cell cycles of Xenopus; regulation of cell cycle length by Xe-wee1 and Mos."
Murakami M.S., Vande Woude G.F.
Development 125:237-248(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, MUTAGENESIS OF LYS-242.
[2]NIH - Xenopus Gene Collection (XGC) project
Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Gastrula.
[3]"Tome-1, a trigger of mitotic entry, is degraded during G1 via the APC."
Ayad N.G., Rankin S., Murakami M., Jebanathirajah J., Gygi S.P., Kirschner M.W.
Cell 113:101-113(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-38, UBIQUITINATION, INTERACTION WITH CDCA3, MUTAGENESIS OF SER-38.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF035443 mRNA. Translation: AAB99952.1.
BC169846 mRNA. Translation: AAI69846.1.
BC169848 mRNA. Translation: AAI69848.1.
RefSeqNP_001081724.1. NM_001088255.1.
UniGeneXl.4222.

3D structure databases

ProteinModelPortalO57473.
SMRO57473. Positions 205-483.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID398017.
KEGGxla:398017.

Organism-specific databases

CTD494551.

Phylogenomic databases

HOVERGENHBG005050.
KOK06632.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR017164. Wee1-like_protein_kinase.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFPIRSF037281. Wee1-like_protein_kinase. 1 hit.
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameWEE2B_XENLA
AccessionPrimary (citable) accession number: O57473
Secondary accession number(s): B7ZQL0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: June 1, 1998
Last modified: May 14, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families