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O57473

- WEE2B_XENLA

UniProt

O57473 - WEE2B_XENLA

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Protein

Wee1-like protein kinase 2-B

Gene

wee2-b

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Oocyte and early embryo-specific protein tyrosine kinase that phosphorylates and inhibits cdk1 and acts as a regulator of meiosis in oocytes. Required to ensure the meiotic cell cycle in oocytes by phosphorylating cdk1 at 'Tyr-15', leading to inhibit cdk1 activity and prevent meiosis.1 Publication

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.1 PublicationPROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei242 – 2421ATPPROSITE-ProRule annotation
Active sitei340 – 3401Proton acceptorPROSITE-ProRule annotation
Metal bindingi345 – 3451Magnesium; via carbonyl oxygenBy similarity
Metal bindingi377 – 3771Magnesium; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi219 – 2279ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
  4. protein serine/threonine kinase activity Source: InterPro

GO - Biological processi

  1. meiotic nuclear division Source: UniProtKB-KW
  2. mitotic nuclear division Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Meiosis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Wee1-like protein kinase 2-B (EC:2.7.10.2)
Alternative name(s):
Wee1-like protein kinase 1
Xe-wee1
Gene namesi
Name:wee2-b
Synonyms:wee1
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi38 – 381S → A: Strongly reduces the interaction with cdca3. 1 Publication
Mutagenesisi242 – 2421K → M: Dominant-negative mutant; reduces the level of cdk1 tyrosine phosphorylation and shortened the length of the cell cycle. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 554554Wee1-like protein kinase 2-BPRO_0000409529Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei38 – 381Phosphoserine1 Publication

Post-translational modificationi

Ubiquitinated and degraded at the onset of G2/M phase.1 Publication
Phosphorylated during M and G1 phases. Interacts with cdca3 when phosphorylated at Ser-38.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Expressioni

Developmental stagei

Absent in stage VI oocytes and is expressed from meiosis II until gastrulation.1 Publication

Interactioni

Subunit structurei

Interacts with cdca3.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO57473.
SMRiO57473. Positions 205-483.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini213 – 487275Protein kinasePROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili490 – 51627Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

HOVERGENiHBG005050.
KOiK06632.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR017164. Wee1-like_protein_kinase.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF037281. Wee1-like_protein_kinase. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O57473-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRMAMSCGGR LVQRLDFSSS EEEDGLSNRI NEAPQKGSPV SSWRTNNCPF
60 70 80 90 100
PITPQRNERG LSPTQELSPS SDYSPDPSDK GVGGECPGTP LHYSTWKKLK
110 120 130 140 150
LCDTPYTPKS LLYKTLPSPG SRVHCRGQRL LRFVAGTGAE TEDPTLVNVN
160 170 180 190 200
PFTPQSYRQT HFQPNGKRKE RPEDDCSSDS QMKFTDKEHP AVFQSKRFVL
210 220 230 240 250
RETNMESRYK TEFLEIEKIG AGEFGSVFKC VKRLDGCFYV IKRSKKPLAG
260 270 280 290 300
STDEQLALRE VYAHAVLGHH PHVVRYYSAW AEDDHMIIQN EYCNGGSLQD
310 320 330 340 350
LIMENNKKGQ FVPEQELKEI LLQVSMGLKY IHGSGLVHMD IKPSNIFICR
360 370 380 390 400
KQTEVGEDES DGEDDVASAS VLYKIGDLGH VTSILNPQVE EGDSRFLANE
410 420 430 440 450
ILQEDYRQLP KADIFALGLT IALAAGAAPL PCNEDGWHHI RKGNLPHIPQ
460 470 480 490 500
PLTPAFLALL KLLVHPDPAT RPPATSLAKN SVLRRCVGKA AELQKQLNVE
510 520 530 540 550
KFKTAMLERE LQAAKLAQDE CLDLPPVSGF SCRGRKRLVG AKNARSLSFT

CGGY
Length:554
Mass (Da):61,666
Last modified:June 1, 1998 - v1
Checksum:i30021FB7A95C5F0D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti190 – 1901P → S in AAI69848. 1 PublicationCurated
Sequence conflicti190 – 1901P → S in AAI69846. 1 PublicationCurated
Sequence conflicti367 – 3671A → S in AAI69848. 1 PublicationCurated
Sequence conflicti367 – 3671A → S in AAI69846. 1 PublicationCurated
Sequence conflicti469 – 4691A → V in AAI69848. 1 PublicationCurated
Sequence conflicti469 – 4691A → V in AAI69846. 1 PublicationCurated
Sequence conflicti475 – 4751T → A in AAI69848. 1 PublicationCurated
Sequence conflicti475 – 4751T → A in AAI69846. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF035443 mRNA. Translation: AAB99952.1.
BC169846 mRNA. Translation: AAI69846.1.
BC169848 mRNA. Translation: AAI69848.1.
RefSeqiNP_001081724.1. NM_001088255.1.
UniGeneiXl.4222.

Genome annotation databases

GeneIDi398017.
KEGGixla:398017.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF035443 mRNA. Translation: AAB99952.1 .
BC169846 mRNA. Translation: AAI69846.1 .
BC169848 mRNA. Translation: AAI69848.1 .
RefSeqi NP_001081724.1. NM_001088255.1.
UniGenei Xl.4222.

3D structure databases

ProteinModelPortali O57473.
SMRi O57473. Positions 205-483.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 398017.
KEGGi xla:398017.

Organism-specific databases

CTDi 494551.

Phylogenomic databases

HOVERGENi HBG005050.
KOi K06632.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR017164. Wee1-like_protein_kinase.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
PIRSFi PIRSF037281. Wee1-like_protein_kinase. 1 hit.
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the early embryonic cell cycles of Xenopus; regulation of cell cycle length by Xe-wee1 and Mos."
    Murakami M.S., Vande Woude G.F.
    Development 125:237-248(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, MUTAGENESIS OF LYS-242.
  2. NIH - Xenopus Gene Collection (XGC) project
    Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Gastrula.
  3. "Tome-1, a trigger of mitotic entry, is degraded during G1 via the APC."
    Ayad N.G., Rankin S., Murakami M., Jebanathirajah J., Gygi S.P., Kirschner M.W.
    Cell 113:101-113(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-38, UBIQUITINATION, INTERACTION WITH CDCA3, MUTAGENESIS OF SER-38.

Entry informationi

Entry nameiWEE2B_XENLA
AccessioniPrimary (citable) accession number: O57473
Secondary accession number(s): B7ZQL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: June 1, 1998
Last modified: October 29, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Was initially assigned as wee1 (PubMed:9486797). However, it corresponds to the meiosis-specific protein WEE2 in mammals.1 Publication

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3