Chordin precursor - Danio rerio (Zebrafish)

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O57472 (CHRD_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 85. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Chordin

Alternative name(s):
Protein chordino

Gene names

Name:	chd
Synonyms:	chrd

Organism

Danio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	940 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Dorsalizing factor. Key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta family bone morphogenetic proteins (BMPs) and sequestering them in latent complexes By similarity. Ref.1
Subunit structure	Interacts with twsg1 and/or bmp4 By similarity.
Subcellular location	Secreted By similarity.
Tissue specificity	During gastrulation, levels are highest in the organizer region. Also present in the developing brain and in paraxial mesoderm and ectoderm. Ref.1
Developmental stage	First detected shortly after the midbastula transition. Levels increase during gastrulation, persist through early somatogenesis, but then decrease and are gone by 24 hours. Ref.1
Post-translational modification	Cleaved by tolloid proteases; cleavage participates in dorsoventral patterning during early development By similarity.
Sequence similarities	Belongs to the chordin family. Contains 4 CHRD domains. Contains 4 VWFC domains.

Ontologies

Keywords
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Developmental protein
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Kupffer's vesicle development Inferred from mutant phenotype PubMed 20925124. Source: ZFIN anterior/posterior axis specification Inferred from direct assay PubMed 19369398. Source: ZFIN caudal fin morphogenesis Inferred from mutant phenotype PubMed 15604098. Source: ZFIN determination of ventral identity Inferred from mutant phenotype PubMed 15525664 PubMed 20935645. Source: ZFIN dorsal convergence Inferred from genetic interaction PubMed 17331724. Source: ZFIN embryonic hemopoiesis Inferred from mutant phenotype PubMed 15572152 PubMed 15604098 PubMed 16413388 PubMed 18718762. Source: ZFIN heart looping Inferred from mutant phenotype PubMed 9334285. Source: ZFIN negative regulation of BMP signaling pathway Inferred from direct assay PubMed 16959242. Source: ZFIN otic placode formation Inferred from genetic interaction PubMed 19418450. Source: ZFIN regulation of BMP signaling pathway involved in heart jogging Inferred from mutant phenotype PubMed 9334285. Source: ZFIN specification of organ position Inferred from mutant phenotype PubMed 10903181. Source: ZFIN
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

Potential

Chain

20 – 940

921

Chordin

PRO_0000005366

Regions

Domain

42 – 118

VWFC 1

Domain

162 – 277

116

CHRD 1

Domain

279 – 398

120

CHRD 2

Domain

404 – 519

116

CHRD 3

Domain

525 – 652

128

CHRD 4

Domain

689 – 748

VWFC 2

Domain

767 – 836

VWFC 3

Domain

855 – 919

VWFC 4

Amino acid modifications

Glycosylation

347

N-linked (GlcNAc...) Potential

Glycosylation

430

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

O57472 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: B855CAF84F4623AC
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FASTA

940

104,999

        10         20         30         40         50         60 
MMEGLLWILL SVIIASVHGS RLKTPALPIQ PEREPMISKG LSGCSFGGRF YSLEDTWHPD 

        70         80         90        100        110        120 
LGEPFGVMHC VMCHCEPQRS RRGKVFGKVS CRNMKQDCPD PTCDDPVLLP GHCCKTCPKG 

       130        140        150        160        170        180 
DSGRKEVESL FDFFQEKDDD LHKSYNDRSY ISSEDTSTRD STTTDFVALL TGVTDSWLPS 

       190        200        210        220        230        240 
SSGVARARFT LSRTSLTFSI TFQRINRPSL IAFLDTDGNT AFEFRVPQAD NDMICGIWKN 

       250        260        270        280        290        300 
VPKPHMRQLE AEQLHVSMTT ADNRKEELQG RIIKHRALFA ETFSAILTSD EVHSGMGGIA 

       310        320        330        340        350        360 
MLTLSDTENN LHFILIMQGL VPPGSSKVPV RVKLQYRQHL LREIRANITA DDSDFAEVLA 

       370        380        390        400        410        420 
DLNSRELFWL SRGQLQISVQ TEGQTLRHIS GFISGRRSCD TLQSVLSSGA ALTAGQTGGV 

       430        440        450        460        470        480 
GSAVFTLHPN GSLDYQLLVA GLSSAVLSVS IEMKPRRRNK RSVLYELSAV FTDQRAAGSC 

       490        500        510        520        530        540 
GRVEARHTHM LLQNELFINI ATALQPDGEL RGQIRLLPYN GLDARRNELP VPLAGVLVSP 

       550        560        570        580        590        600 
PVRTGAAGHA WVSVDPQCHL HYEIIVNGLS KSEDASISAH LHGLAEIGEM DDSSTNHKRL 

       610        620        630        640        650        660 
LTGFYGQQAQ GVLKDISVEL LRHLNEGTAY LQVSTKMNPR GEIRGRIHVP NHCESPAPRA 

       670        680        690        700        710        720 
EFLEEPEFED LLFTREPTEL RKDTHTHVHS CFFEGEQHTH GSQWTPQYNT CFTCTCQKKT 

       730        740        750        760        770        780 
VICDPVMCPT LSCTHTVQPE DQCCPICEEK KESKETAAVE KVEENPEGCY FEGDQKMHAP 

       790        800        810        820        830        840 
GTTWHPFVPP FGYIKCAVCT CKGSTGEVHC EKVTCPPLTC SRPIRRNPSD CCKECPPEET 

       850        860        870        880        890        900 
PPLEDEEMMQ ADGTRLCKFG KNYYQNSEHW HPSVPLVGEM KCITCWCDHG VTKCQRKQCP 

       910        920        930        940 
LLSCRNPIRT EGKCCPECIE DFMEKEEMAK MAEKKKSWRH

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References

[1]	"Differential regulation of chordin expression domains in mutant zebrafish." Miller-Bertoglio V.E., Fisher S., Sanchez A., Mullins M.C., Halpern M.E. Dev. Biol. 192:537-550(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Tissue: Embryo.
[2]	"Regulation of chordino." Fujii R., Hibi M., Hirano T., Shimizu T. Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-42.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF034606 mRNA. Translation: AAB93485.1. AB043968 Genomic DNA. Translation: BAB18642.1.
IPI	IPI00512647.
RefSeq	NP_571048.1. NM_130973.2.
UniGene	Dr.75804.
3D structure databases
ProteinModelPortal	O57472.
ModBase	Search...
Protein-protein interaction databases
STRING	7955.ENSDARP00000045109.
Proteomic databases
PRIDE	O57472.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	30161.
KEGG	dre:30161.
Organism-specific databases
CTD	30161.
ZFIN	ZDB-GENE-990415-33. chd.
Phylogenomic databases
eggNOG	NOG85639.
HOGENOM	HOG000234370.
HOVERGEN	HBG005330.
InParanoid	O57472.
KO	K04657.
OrthoDB	EOG4T1HKS.
Family and domain databases
InterPro	IPR016353. Chordin. IPR010895. CHRD. IPR001007. VWF_C. [Graphical view]
Pfam	PF07452. CHRD. 4 hits. PF00093. VWC. 4 hits. [Graphical view]
PIRSF	PIRSF002496. Chordin. 1 hit.
SMART	SM00754. CHRD. 4 hits. SM00214. VWC. 4 hits. [Graphical view]
PROSITE	PS50933. CHRD. 4 hits. PS01208. VWFC_1. 3 hits. PS50184. VWFC_2. 4 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	20806631.

Entry information

Entry name

CHRD_DANRE

Accession

Primary (citable) accession number: O57472
Secondary accession number(s): Q9DED8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	June 1, 1998
Last modified:	April 3, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Other

Entry information

Relevant documents