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O57472 (CHRD_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chordin
Alternative name(s):
Protein chordino
Gene names
Name:chd
Synonyms:chrd
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Protein attributes

Sequence length940 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Dorsalizing factor. Key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta family bone morphogenetic proteins (BMPs) and sequestering them in latent complexes By similarity. Ref.1

Subunit structure

Interacts with twsg1 and/or bmp4 By similarity.

Subcellular location

Secreted By similarity.

Tissue specificity

During gastrulation, levels are highest in the organizer region. Also present in the developing brain and in paraxial mesoderm and ectoderm. Ref.1

Developmental stage

First detected shortly after the midbastula transition. Levels increase during gastrulation, persist through early somatogenesis, but then decrease and are gone by 24 hours. Ref.1

Post-translational modification

Cleaved by tolloid proteases; cleavage participates in dorsoventral patterning during early development By similarity.

Sequence similarities

Belongs to the chordin family.

Contains 4 CHRD domains.

Contains 4 VWFC domains.

Ontologies

Keywords
   Cellular componentSecreted
   DomainRepeat
Signal
   Molecular functionDevelopmental protein
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processKupffer's vesicle development

Inferred from mutant phenotype PubMed 20925124. Source: ZFIN

anterior/posterior axis specification

Inferred from direct assay PubMed 19369398. Source: ZFIN

caudal fin morphogenesis

Inferred from mutant phenotype PubMed 15604098. Source: ZFIN

determination of heart left/right asymmetry

Inferred from mutant phenotype PubMed 20925124. Source: ZFIN

determination of left/right symmetry

Inferred from mutant phenotype PubMed 10903181PubMed 9334285. Source: ZFIN

determination of ventral identity

Inferred from mutant phenotype PubMed 15525664PubMed 20935645. Source: ZFIN

dorsal convergence

Inferred from genetic interaction PubMed 17331724. Source: ZFIN

dorsal/ventral pattern formation

Inferred from mutant phenotype PubMed 11969259PubMed 15151985PubMed 16890217PubMed 18248235PubMed 19369398PubMed 20226780PubMed 20886103PubMed 21558420PubMed 9395394. Source: ZFIN

embryonic heart tube morphogenesis

Inferred from mutant phenotype PubMed 9334285. Source: ZFIN

embryonic hemopoiesis

Inferred from mutant phenotype PubMed 15572152PubMed 15604098PubMed 16413388PubMed 18718762. Source: ZFIN

heart looping

Inferred from mutant phenotype PubMed 9334285. Source: ZFIN

negative regulation of BMP signaling pathway

Inferred from direct assay PubMed 16959242. Source: ZFIN

otic placode formation

Inferred from genetic interaction PubMed 19418450. Source: ZFIN

regulation of BMP signaling pathway involved in heart jogging

Inferred from mutant phenotype PubMed 9334285. Source: ZFIN

somitogenesis

Inferred from mutant phenotype PubMed 23246591. Source: ZFIN

specification of organ position

Inferred from mutant phenotype PubMed 10903181. Source: ZFIN

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionprotein binding

Inferred from physical interaction PubMed 20886103. Source: ZFIN

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 940921Chordin
PRO_0000005366

Regions

Domain42 – 11877VWFC 1
Domain162 – 277116CHRD 1
Domain279 – 398120CHRD 2
Domain404 – 519116CHRD 3
Domain525 – 652128CHRD 4
Domain689 – 74860VWFC 2
Domain767 – 83670VWFC 3
Domain855 – 91965VWFC 4

Amino acid modifications

Glycosylation3471N-linked (GlcNAc...) Potential
Glycosylation4301N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
O57472 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: B855CAF84F4623AC

FASTA940104,999
        10         20         30         40         50         60 
MMEGLLWILL SVIIASVHGS RLKTPALPIQ PEREPMISKG LSGCSFGGRF YSLEDTWHPD 

        70         80         90        100        110        120 
LGEPFGVMHC VMCHCEPQRS RRGKVFGKVS CRNMKQDCPD PTCDDPVLLP GHCCKTCPKG 

       130        140        150        160        170        180 
DSGRKEVESL FDFFQEKDDD LHKSYNDRSY ISSEDTSTRD STTTDFVALL TGVTDSWLPS 

       190        200        210        220        230        240 
SSGVARARFT LSRTSLTFSI TFQRINRPSL IAFLDTDGNT AFEFRVPQAD NDMICGIWKN 

       250        260        270        280        290        300 
VPKPHMRQLE AEQLHVSMTT ADNRKEELQG RIIKHRALFA ETFSAILTSD EVHSGMGGIA 

       310        320        330        340        350        360 
MLTLSDTENN LHFILIMQGL VPPGSSKVPV RVKLQYRQHL LREIRANITA DDSDFAEVLA 

       370        380        390        400        410        420 
DLNSRELFWL SRGQLQISVQ TEGQTLRHIS GFISGRRSCD TLQSVLSSGA ALTAGQTGGV 

       430        440        450        460        470        480 
GSAVFTLHPN GSLDYQLLVA GLSSAVLSVS IEMKPRRRNK RSVLYELSAV FTDQRAAGSC 

       490        500        510        520        530        540 
GRVEARHTHM LLQNELFINI ATALQPDGEL RGQIRLLPYN GLDARRNELP VPLAGVLVSP 

       550        560        570        580        590        600 
PVRTGAAGHA WVSVDPQCHL HYEIIVNGLS KSEDASISAH LHGLAEIGEM DDSSTNHKRL 

       610        620        630        640        650        660 
LTGFYGQQAQ GVLKDISVEL LRHLNEGTAY LQVSTKMNPR GEIRGRIHVP NHCESPAPRA 

       670        680        690        700        710        720 
EFLEEPEFED LLFTREPTEL RKDTHTHVHS CFFEGEQHTH GSQWTPQYNT CFTCTCQKKT 

       730        740        750        760        770        780 
VICDPVMCPT LSCTHTVQPE DQCCPICEEK KESKETAAVE KVEENPEGCY FEGDQKMHAP 

       790        800        810        820        830        840 
GTTWHPFVPP FGYIKCAVCT CKGSTGEVHC EKVTCPPLTC SRPIRRNPSD CCKECPPEET 

       850        860        870        880        890        900 
PPLEDEEMMQ ADGTRLCKFG KNYYQNSEHW HPSVPLVGEM KCITCWCDHG VTKCQRKQCP 

       910        920        930        940 
LLSCRNPIRT EGKCCPECIE DFMEKEEMAK MAEKKKSWRH 

« Hide

References

[1]"Differential regulation of chordin expression domains in mutant zebrafish."
Miller-Bertoglio V.E., Fisher S., Sanchez A., Mullins M.C., Halpern M.E.
Dev. Biol. 192:537-550(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Tissue: Embryo.
[2]"Regulation of chordino."
Fujii R., Hibi M., Hirano T., Shimizu T.
Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-42.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF034606 mRNA. Translation: AAB93485.1.
AB043968 Genomic DNA. Translation: BAB18642.1.
RefSeqNP_571048.1. NM_130973.2.
UniGeneDr.75804.

3D structure databases

ProteinModelPortalO57472.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING7955.ENSDARP00000045109.

Proteomic databases

PRIDEO57472.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID30161.
KEGGdre:30161.

Organism-specific databases

CTD30161.
ZFINZDB-GENE-990415-33. chd.

Phylogenomic databases

eggNOGNOG85639.
HOGENOMHOG000234370.
HOVERGENHBG005330.
InParanoidO57472.
KOK04657.
PhylomeDBO57472.

Family and domain databases

InterProIPR016353. Chordin.
IPR010895. CHRD.
IPR001007. VWF_C.
[Graphical view]
PfamPF07452. CHRD. 4 hits.
PF00093. VWC. 4 hits.
[Graphical view]
PIRSFPIRSF002496. Chordin. 1 hit.
SMARTSM00754. CHRD. 4 hits.
SM00214. VWC. 4 hits.
[Graphical view]
PROSITEPS50933. CHRD. 4 hits.
PS01208. VWFC_1. 3 hits.
PS50184. VWFC_2. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio20806631.
PROO57472.

Entry information

Entry nameCHRD_DANRE
AccessionPrimary (citable) accession number: O57472
Secondary accession number(s): Q9DED8
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: June 1, 1998
Last modified: June 11, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families