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O57460

- TLL1_DANRE

UniProt

O57460 - TLL1_DANRE

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Protein

Dorsal-ventral patterning tolloid-like protein 1

Gene

tll1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Required for patterning ventral tissues of the tail. May increase bone morphogenetic protein (BMP) activity at the end of gastrulation by proteolytic cleavage of chordin and release of BMP from inactive complexes.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi249 – 2491Zinc; catalyticPROSITE-ProRule annotation
Active sitei250 – 2501PROSITE-ProRule annotation
Metal bindingi253 – 2531Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi259 – 2591Zinc; catalyticPROSITE-ProRule annotation

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. metalloendopeptidase activity Source: InterPro
  3. metallopeptidase activity Source: ZFIN
  4. peptidase activity Source: ZFIN
  5. zinc ion binding Source: InterPro

GO - Biological processi

  1. blood vessel development Source: ZFIN
  2. determination of ventral identity Source: ZFIN
  3. dorsal/ventral pattern formation Source: ZFIN
  4. embryonic caudal fin morphogenesis Source: ZFIN
  5. embryonic hemopoiesis Source: ZFIN
  6. endothelial cell development Source: ZFIN
  7. mesoderm formation Source: ZFIN
  8. positive regulation of BMP signaling pathway Source: ZFIN
  9. post-anal tail morphogenesis Source: ZFIN
  10. proteolysis Source: ZFIN
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_212272. Anchoring fibril formation.
REACT_218692. Crosslinking of collagen fibrils.
REACT_222767. Collagen biosynthesis and modifying enzymes.

Protein family/group databases

MEROPSiM12.016.

Names & Taxonomyi

Protein namesi
Recommended name:
Dorsal-ventral patterning tolloid-like protein 1 (EC:3.4.24.-)
Alternative name(s):
Mini fin protein
Gene namesi
Name:tll1
Synonyms:mfn, tld, tolloid
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437: Chromosome 1

Organism-specific databases

ZFINiZDB-GENE-041020-1. tll1.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: ZFIN
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Sequence AnalysisAdd
BLAST
Propeptidei33 – 156124Sequence AnalysisPRO_0000028901Add
BLAST
Chaini157 – 1022866Dorsal-ventral patterning tolloid-like protein 1PRO_0000028902Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi129 – 1291N-linked (GlcNAc...)Sequence Analysis
Glycosylationi178 – 1781N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi358 ↔ 384By similarity
Glycosylationi368 – 3681N-linked (GlcNAc...)Sequence Analysis
Glycosylationi399 – 3991N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi411 ↔ 433By similarity
Disulfide bondi471 ↔ 497By similarity
Disulfide bondi524 ↔ 546By similarity
Disulfide bondi587 ↔ 599By similarity
Disulfide bondi595 ↔ 608By similarity
Disulfide bondi610 ↔ 623By similarity
Disulfide bondi627 ↔ 653By similarity
Glycosylationi635 – 6351N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi680 ↔ 702By similarity
Disulfide bondi743 ↔ 754By similarity
Disulfide bondi750 ↔ 763By similarity
Disulfide bondi765 ↔ 778By similarity
Disulfide bondi783 ↔ 809By similarity
Disulfide bondi836 ↔ 858By similarity
Disulfide bondi896 ↔ 926By similarity
Disulfide bondi953 ↔ 975By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Expressioni

Tissue specificityi

During gastrulation, accumulates around the closing blastopore with greater expression ventrally. At the animal pole, expressed in the ectoderm flanking the anterior neural plate. At the 10-somite stage, expressed in the developing tailbud and cranial neural crest. At the 20-somite stage, also expressed in the hematopoietic system.2 Publications

Gene expression databases

BgeeiO57460.

Structurei

3D structure databases

ProteinModelPortaliO57460.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini358 – 470113CUB 1PROSITE-ProRule annotationAdd
BLAST
Domaini471 – 583113CUB 2PROSITE-ProRule annotationAdd
BLAST
Domaini583 – 62442EGF-like 1; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini627 – 739113CUB 3PROSITE-ProRule annotationAdd
BLAST
Domaini739 – 77941EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini783 – 895113CUB 4PROSITE-ProRule annotationAdd
BLAST
Domaini896 – 1012117CUB 5PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni157 – 357201MetalloproteaseBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase M12A family.Curated
Contains 5 CUB domains.PROSITE-ProRule annotation
Contains 2 EGF-like domains.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiNOG70307.
GeneTreeiENSGT00760000119018.
HOGENOMiHOG000236339.
HOVERGENiHBG004859.
InParanoidiO57460.
KOiK09608.
OMAiGEPYDFD.
OrthoDBiEOG7N8ZTV.
PhylomeDBiO57460.
TreeFamiTF314351.

Family and domain databases

Gene3Di2.60.120.290. 5 hits.
3.40.390.10. 1 hit.
InterProiIPR015446. BMP_1/tolloid-like.
IPR000859. CUB_dom.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR024079. MetalloPept_cat_dom.
IPR001506. Peptidase_M12A.
IPR006026. Peptidase_Metallo.
[Graphical view]
PfamiPF01400. Astacin. 1 hit.
PF00431. CUB. 5 hits.
[Graphical view]
PIRSFiPIRSF001199. BMP_1/tolloid-like. 1 hit.
PRINTSiPR00480. ASTACIN.
SMARTiSM00042. CUB. 5 hits.
SM00179. EGF_CA. 2 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 5 hits.
PROSITEiPS00010. ASX_HYDROXYL. 2 hits.
PS01180. CUB. 5 hits.
PS01186. EGF_2. 2 hits.
PS50026. EGF_3. 2 hits.
PS01187. EGF_CA. 2 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O57460-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDYLYSALTS KMNWIALLLA GLTFCCKVSV HSCLDYDDSY DYYEEEKTET
60 70 80 90 100
IDYKDPCKAA VFWGDIALDD EDLKMFHIDG TIDLKQQTHG RQGHTSGGLG
110 120 130 140 150
EHVPTKKRGS LYLLLDRIRR LGFESWPVNS SKDVSSIKTG IRRVNSARNV
160 170 180 190 200
KSRVPRAATS RAEKIWPGGV IPYVIGGNFT GSQRAMLKQA MRHWEKQTCV
210 220 230 240 250
TFIEKTDEES YIVFTYRPCG CCSYVGRRGN GPQAISIGKN CDKFGIVVHE
260 270 280 290 300
LGHVIGFWHE HTRPDRDDHV TIIRDNIQPG QEYNFIKMEP GDVNSLGEPY
310 320 330 340 350
DFDSIMHYAR NTFSRGMFLD TILPSRDENG VRPAIGQRTR LSKGDISQAK
360 370 380 390 400
KLYRCPACGE TLQDSVGNFS SPGYPNGYPS YTHCVWRISV TPGEKIVLNF
410 420 430 440 450
TTMDLYKSSL CWYDYIEVRD GYWRKAPLLG RFCGDKIPEV LVSTDSRMWI
460 470 480 490 500
EFRSSSNWVG KGFAAVYEAI CGGEISKDSG QIQSPNYPDD YRPSKECVWR
510 520 530 540 550
ITVSEGYSVG LSFQVFEIER HDSCAYDYLE VRDGLSENSP LIGRFCGYDK
560 570 580 590 600
PEDIRSTSNN LWMKFVSDGT VNKAGFAANF FKEEDECLKP DNGGCEQRCV
610 620 630 640 650
NTLGSFKCAC DPGYELAPDK KSCEAACGGL LTKLNGTITT PGWPKEYPPN
660 670 680 690 700
KNCVWQVVAP TQYRISMQFE AFELEGNEVC KYDYVEVRSG LSSDSKLHGK
710 720 730 740 750
YCGTEVPEVI TSQYNNMRIE FKSDNTVSKK GFKAHFFSDK DECSKDNGGC
760 770 780 790 800
QHECINTIGS YVCQCRNGFI LHENKHDCKE AECEHKIHST TGTISSPNWP
810 820 830 840 850
DKYPSRKECT WDITATPGHR VKISFNEFEI EQHQECAYDH LEAFDGDSDK
860 870 880 890 900
TPILSRLCGN KIPEPLISTG NKMYLRFISD ASVQRKGFQA THSTECGGRL
910 920 930 940 950
KAEARQKNLY SHAQFGDNNY PGHTDCEWLI VAESGYGIEL TFTTFEVEEE
960 970 980 990 1000
ADCGYDYIEL YDGYDTGAHK IGRFCGSGPR EELYSAGDAV LIHFHSDDTI
1010 1020
SKKGFHIRYT STKFQEALHT RK
Length:1,022
Mass (Da):115,536
Last modified:June 1, 1998 - v1
Checksum:iA68CA1D0E41793F9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF027596 mRNA. Translation: AAC60304.1.
RefSeqiNP_571085.1. NM_131010.1.
UniGeneiDr.75803.

Genome annotation databases

EnsembliENSDART00000054472; ENSDARP00000054471; ENSDARG00000037429.
GeneIDi474335.
KEGGidre:474335.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF027596 mRNA. Translation: AAC60304.1 .
RefSeqi NP_571085.1. NM_131010.1.
UniGenei Dr.75803.

3D structure databases

ProteinModelPortali O57460.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi M12.016.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSDART00000054472 ; ENSDARP00000054471 ; ENSDARG00000037429 .
GeneIDi 474335.
KEGGi dre:474335.

Organism-specific databases

CTDi 7092.
ZFINi ZDB-GENE-041020-1. tll1.

Phylogenomic databases

eggNOGi NOG70307.
GeneTreei ENSGT00760000119018.
HOGENOMi HOG000236339.
HOVERGENi HBG004859.
InParanoidi O57460.
KOi K09608.
OMAi GEPYDFD.
OrthoDBi EOG7N8ZTV.
PhylomeDBi O57460.
TreeFami TF314351.

Enzyme and pathway databases

Reactomei REACT_212272. Anchoring fibril formation.
REACT_218692. Crosslinking of collagen fibrils.
REACT_222767. Collagen biosynthesis and modifying enzymes.

Miscellaneous databases

NextBioi 20850399.
PROi O57460.

Gene expression databases

Bgeei O57460.

Family and domain databases

Gene3Di 2.60.120.290. 5 hits.
3.40.390.10. 1 hit.
InterProi IPR015446. BMP_1/tolloid-like.
IPR000859. CUB_dom.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR024079. MetalloPept_cat_dom.
IPR001506. Peptidase_M12A.
IPR006026. Peptidase_Metallo.
[Graphical view ]
Pfami PF01400. Astacin. 1 hit.
PF00431. CUB. 5 hits.
[Graphical view ]
PIRSFi PIRSF001199. BMP_1/tolloid-like. 1 hit.
PRINTSi PR00480. ASTACIN.
SMARTi SM00042. CUB. 5 hits.
SM00179. EGF_CA. 2 hits.
SM00235. ZnMc. 1 hit.
[Graphical view ]
SUPFAMi SSF49854. SSF49854. 5 hits.
PROSITEi PS00010. ASX_HYDROXYL. 2 hits.
PS01180. CUB. 5 hits.
PS01186. EGF_2. 2 hits.
PS50026. EGF_3. 2 hits.
PS01187. EGF_CA. 2 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cleavage of the BMP-4 antagonist chordin by zebrafish Tolloid."
    Blader P., Rastegar S., Fischer N., Straehle U.
    Science 278:1937-1940(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Tissue: Embryo.
  2. "The role of tolloid/mini fin in dorsoventral pattern formation of the zebrafish embryo."
    Connors S.A., Trout J., Ekker M., Mullins M.C.
    Development 126:3119-3130(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiTLL1_DANRE
AccessioniPrimary (citable) accession number: O57460
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: June 1, 1998
Last modified: October 29, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3