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O57460

- TLL1_DANRE

UniProt

O57460 - TLL1_DANRE

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Protein
Dorsal-ventral patterning tolloid-like protein 1
Gene
tll1, mfn, tld, tolloid
Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Required for patterning ventral tissues of the tail. May increase bone morphogenetic protein (BMP) activity at the end of gastrulation by proteolytic cleavage of chordin and release of BMP from inactive complexes.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi249 – 2491Zinc; catalytic By similarity
Active sitei250 – 2501 By similarity
Metal bindingi253 – 2531Zinc; catalytic By similarity
Metal bindingi259 – 2591Zinc; catalytic By similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. metalloendopeptidase activity Source: InterPro
  3. metallopeptidase activity Source: ZFIN
  4. peptidase activity Source: ZFIN
  5. protein binding Source: ZFIN
  6. zinc ion binding Source: InterPro

GO - Biological processi

  1. blood vessel development Source: ZFIN
  2. determination of ventral identity Source: ZFIN
  3. dorsal/ventral pattern formation Source: ZFIN
  4. embryonic caudal fin morphogenesis Source: ZFIN
  5. embryonic hemopoiesis Source: ZFIN
  6. endothelial cell development Source: ZFIN
  7. mesoderm formation Source: ZFIN
  8. positive regulation of BMP signaling pathway Source: ZFIN
  9. post-anal tail morphogenesis Source: ZFIN
  10. proteolysis Source: ZFIN
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_212272. Anchoring fibril formation.
REACT_218692. Crosslinking of collagen fibrils.
REACT_222767. Collagen biosynthesis and modifying enzymes.

Protein family/group databases

MEROPSiM12.016.

Names & Taxonomyi

Protein namesi
Recommended name:
Dorsal-ventral patterning tolloid-like protein 1 (EC:3.4.24.-)
Alternative name(s):
Mini fin protein
Gene namesi
Name:tll1
Synonyms:mfn, tld, tolloid
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437: Chromosome 1

Organism-specific databases

ZFINiZDB-GENE-041020-1. tll1.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: ZFIN
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232 Reviewed prediction
Add
BLAST
Propeptidei33 – 156124 Reviewed prediction
PRO_0000028901Add
BLAST
Chaini157 – 1022866Dorsal-ventral patterning tolloid-like protein 1
PRO_0000028902Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi129 – 1291N-linked (GlcNAc...) Reviewed prediction
Glycosylationi178 – 1781N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi358 ↔ 384 By similarity
Glycosylationi368 – 3681N-linked (GlcNAc...) Reviewed prediction
Glycosylationi399 – 3991N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi411 ↔ 433 By similarity
Disulfide bondi471 ↔ 497 By similarity
Disulfide bondi524 ↔ 546 By similarity
Disulfide bondi587 ↔ 599 By similarity
Disulfide bondi595 ↔ 608 By similarity
Disulfide bondi610 ↔ 623 By similarity
Disulfide bondi627 ↔ 653 By similarity
Glycosylationi635 – 6351N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi680 ↔ 702 By similarity
Disulfide bondi743 ↔ 754 By similarity
Disulfide bondi750 ↔ 763 By similarity
Disulfide bondi765 ↔ 778 By similarity
Disulfide bondi783 ↔ 809 By similarity
Disulfide bondi836 ↔ 858 By similarity
Disulfide bondi896 ↔ 926 By similarity
Disulfide bondi953 ↔ 975 By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Expressioni

Tissue specificityi

During gastrulation, accumulates around the closing blastopore with greater expression ventrally. At the animal pole, expressed in the ectoderm flanking the anterior neural plate. At the 10-somite stage, expressed in the developing tailbud and cranial neural crest. At the 20-somite stage, also expressed in the hematopoietic system.2 Publications

Gene expression databases

BgeeiO57460.

Structurei

3D structure databases

ProteinModelPortaliO57460.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini358 – 470113CUB 1
Add
BLAST
Domaini471 – 583113CUB 2
Add
BLAST
Domaini583 – 62442EGF-like 1; calcium-binding Reviewed prediction
Add
BLAST
Domaini627 – 739113CUB 3
Add
BLAST
Domaini739 – 77941EGF-like 2; calcium-binding Reviewed prediction
Add
BLAST
Domaini783 – 895113CUB 4
Add
BLAST
Domaini896 – 1012117CUB 5
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni157 – 357201Metalloprotease By similarity
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase M12A family.
Contains 5 CUB domains.
Contains 2 EGF-like domains.

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiNOG70307.
GeneTreeiENSGT00750000117289.
HOGENOMiHOG000236339.
HOVERGENiHBG004859.
InParanoidiO57460.
KOiK09608.
OMAiGEPYDFD.
OrthoDBiEOG7N8ZTV.
PhylomeDBiO57460.
TreeFamiTF314351.

Family and domain databases

Gene3Di2.60.120.290. 5 hits.
3.40.390.10. 1 hit.
InterProiIPR015446. BMP_1/tolloid-like.
IPR000859. CUB_dom.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR024079. MetalloPept_cat_dom.
IPR001506. Peptidase_M12A.
IPR006026. Peptidase_Metallo.
[Graphical view]
PfamiPF01400. Astacin. 1 hit.
PF00431. CUB. 5 hits.
[Graphical view]
PIRSFiPIRSF001199. BMP_1/tolloid-like. 1 hit.
PRINTSiPR00480. ASTACIN.
SMARTiSM00042. CUB. 5 hits.
SM00179. EGF_CA. 2 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 5 hits.
PROSITEiPS00010. ASX_HYDROXYL. 2 hits.
PS01180. CUB. 5 hits.
PS01186. EGF_2. 2 hits.
PS50026. EGF_3. 2 hits.
PS01187. EGF_CA. 2 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O57460-1 [UniParc]FASTAAdd to Basket

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MDYLYSALTS KMNWIALLLA GLTFCCKVSV HSCLDYDDSY DYYEEEKTET     50
IDYKDPCKAA VFWGDIALDD EDLKMFHIDG TIDLKQQTHG RQGHTSGGLG 100
EHVPTKKRGS LYLLLDRIRR LGFESWPVNS SKDVSSIKTG IRRVNSARNV 150
KSRVPRAATS RAEKIWPGGV IPYVIGGNFT GSQRAMLKQA MRHWEKQTCV 200
TFIEKTDEES YIVFTYRPCG CCSYVGRRGN GPQAISIGKN CDKFGIVVHE 250
LGHVIGFWHE HTRPDRDDHV TIIRDNIQPG QEYNFIKMEP GDVNSLGEPY 300
DFDSIMHYAR NTFSRGMFLD TILPSRDENG VRPAIGQRTR LSKGDISQAK 350
KLYRCPACGE TLQDSVGNFS SPGYPNGYPS YTHCVWRISV TPGEKIVLNF 400
TTMDLYKSSL CWYDYIEVRD GYWRKAPLLG RFCGDKIPEV LVSTDSRMWI 450
EFRSSSNWVG KGFAAVYEAI CGGEISKDSG QIQSPNYPDD YRPSKECVWR 500
ITVSEGYSVG LSFQVFEIER HDSCAYDYLE VRDGLSENSP LIGRFCGYDK 550
PEDIRSTSNN LWMKFVSDGT VNKAGFAANF FKEEDECLKP DNGGCEQRCV 600
NTLGSFKCAC DPGYELAPDK KSCEAACGGL LTKLNGTITT PGWPKEYPPN 650
KNCVWQVVAP TQYRISMQFE AFELEGNEVC KYDYVEVRSG LSSDSKLHGK 700
YCGTEVPEVI TSQYNNMRIE FKSDNTVSKK GFKAHFFSDK DECSKDNGGC 750
QHECINTIGS YVCQCRNGFI LHENKHDCKE AECEHKIHST TGTISSPNWP 800
DKYPSRKECT WDITATPGHR VKISFNEFEI EQHQECAYDH LEAFDGDSDK 850
TPILSRLCGN KIPEPLISTG NKMYLRFISD ASVQRKGFQA THSTECGGRL 900
KAEARQKNLY SHAQFGDNNY PGHTDCEWLI VAESGYGIEL TFTTFEVEEE 950
ADCGYDYIEL YDGYDTGAHK IGRFCGSGPR EELYSAGDAV LIHFHSDDTI 1000
SKKGFHIRYT STKFQEALHT RK 1022
Length:1,022
Mass (Da):115,536
Last modified:June 1, 1998 - v1
Checksum:iA68CA1D0E41793F9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF027596 mRNA. Translation: AAC60304.1.
RefSeqiNP_571085.1. NM_131010.1.
UniGeneiDr.75803.

Genome annotation databases

EnsembliENSDART00000054472; ENSDARP00000054471; ENSDARG00000037429.
GeneIDi474335.
KEGGidre:474335.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF027596 mRNA. Translation: AAC60304.1 .
RefSeqi NP_571085.1. NM_131010.1.
UniGenei Dr.75803.

3D structure databases

ProteinModelPortali O57460.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi M12.016.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSDART00000054472 ; ENSDARP00000054471 ; ENSDARG00000037429 .
GeneIDi 474335.
KEGGi dre:474335.

Organism-specific databases

CTDi 7092.
ZFINi ZDB-GENE-041020-1. tll1.

Phylogenomic databases

eggNOGi NOG70307.
GeneTreei ENSGT00750000117289.
HOGENOMi HOG000236339.
HOVERGENi HBG004859.
InParanoidi O57460.
KOi K09608.
OMAi GEPYDFD.
OrthoDBi EOG7N8ZTV.
PhylomeDBi O57460.
TreeFami TF314351.

Enzyme and pathway databases

Reactomei REACT_212272. Anchoring fibril formation.
REACT_218692. Crosslinking of collagen fibrils.
REACT_222767. Collagen biosynthesis and modifying enzymes.

Miscellaneous databases

NextBioi 20850399.
PROi O57460.

Gene expression databases

Bgeei O57460.

Family and domain databases

Gene3Di 2.60.120.290. 5 hits.
3.40.390.10. 1 hit.
InterProi IPR015446. BMP_1/tolloid-like.
IPR000859. CUB_dom.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR024079. MetalloPept_cat_dom.
IPR001506. Peptidase_M12A.
IPR006026. Peptidase_Metallo.
[Graphical view ]
Pfami PF01400. Astacin. 1 hit.
PF00431. CUB. 5 hits.
[Graphical view ]
PIRSFi PIRSF001199. BMP_1/tolloid-like. 1 hit.
PRINTSi PR00480. ASTACIN.
SMARTi SM00042. CUB. 5 hits.
SM00179. EGF_CA. 2 hits.
SM00235. ZnMc. 1 hit.
[Graphical view ]
SUPFAMi SSF49854. SSF49854. 5 hits.
PROSITEi PS00010. ASX_HYDROXYL. 2 hits.
PS01180. CUB. 5 hits.
PS01186. EGF_2. 2 hits.
PS50026. EGF_3. 2 hits.
PS01187. EGF_CA. 2 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cleavage of the BMP-4 antagonist chordin by zebrafish Tolloid."
    Blader P., Rastegar S., Fischer N., Straehle U.
    Science 278:1937-1940(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Tissue: Embryo.
  2. "The role of tolloid/mini fin in dorsoventral pattern formation of the zebrafish embryo."
    Connors S.A., Trout J., Ekker M., Mullins M.C.
    Development 126:3119-3130(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiTLL1_DANRE
AccessioniPrimary (citable) accession number: O57460
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: June 1, 1998
Last modified: September 3, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi