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Protein

Dorsal-ventral patterning tolloid-like protein 1

Gene

tll1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Required for patterning ventral tissues of the tail. May increase bone morphogenetic protein (BMP) activity at the end of gastrulation by proteolytic cleavage of chordin and release of BMP from inactive complexes.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi249Zinc; catalyticPROSITE-ProRule annotation1
Active sitei250PROSITE-ProRule annotation1
Metal bindingi253Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi259Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • metalloendopeptidase activity Source: InterPro
  • metallopeptidase activity Source: ZFIN
  • peptidase activity Source: ZFIN
  • zinc ion binding Source: InterPro

GO - Biological processi

  • blood vessel development Source: ZFIN
  • determination of ventral identity Source: ZFIN
  • dorsal/ventral pattern formation Source: ZFIN
  • embryonic caudal fin morphogenesis Source: ZFIN
  • embryonic hemopoiesis Source: ZFIN
  • endothelial cell development Source: ZFIN
  • mesoderm formation Source: ZFIN
  • positive regulation of BMP signaling pathway Source: ZFIN
  • post-anal tail morphogenesis Source: ZFIN
  • proteolysis Source: ZFIN
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DRE-1474228. Degradation of the extracellular matrix.
R-DRE-1650814. Collagen biosynthesis and modifying enzymes.
R-DRE-2214320. Anchoring fibril formation.
R-DRE-2243919. Crosslinking of collagen fibrils.

Names & Taxonomyi

Protein namesi
Recommended name:
Dorsal-ventral patterning tolloid-like protein 1 (EC:3.4.24.-)
Alternative name(s):
Mini fin protein
Gene namesi
Name:tll1
Synonyms:mfn, tld, tolloid
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 1

Organism-specific databases

ZFINiZDB-GENE-041020-1. tll1.

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: ZFIN
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 32Sequence analysisAdd BLAST32
PropeptideiPRO_000002890133 – 156Sequence analysisAdd BLAST124
ChainiPRO_0000028902157 – 1022Dorsal-ventral patterning tolloid-like protein 1Add BLAST866

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi129N-linked (GlcNAc...)Sequence analysis1
Glycosylationi178N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi358 ↔ 384By similarity
Glycosylationi368N-linked (GlcNAc...)Sequence analysis1
Glycosylationi399N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi411 ↔ 433By similarity
Disulfide bondi471 ↔ 497By similarity
Disulfide bondi524 ↔ 546By similarity
Disulfide bondi587 ↔ 599By similarity
Disulfide bondi595 ↔ 608By similarity
Disulfide bondi610 ↔ 623By similarity
Disulfide bondi627 ↔ 653By similarity
Glycosylationi635N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi680 ↔ 702By similarity
Disulfide bondi743 ↔ 754By similarity
Disulfide bondi750 ↔ 763By similarity
Disulfide bondi765 ↔ 778By similarity
Disulfide bondi783 ↔ 809By similarity
Disulfide bondi836 ↔ 858By similarity
Disulfide bondi896 ↔ 926By similarity
Disulfide bondi953 ↔ 975By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiO57460.

Expressioni

Tissue specificityi

During gastrulation, accumulates around the closing blastopore with greater expression ventrally. At the animal pole, expressed in the ectoderm flanking the anterior neural plate. At the 10-somite stage, expressed in the developing tailbud and cranial neural crest. At the 20-somite stage, also expressed in the hematopoietic system.2 Publications

Gene expression databases

BgeeiENSDARG00000037429.
ExpressionAtlasiO57460. differential.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000054471.

Structurei

3D structure databases

ProteinModelPortaliO57460.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini358 – 470CUB 1PROSITE-ProRule annotationAdd BLAST113
Domaini471 – 583CUB 2PROSITE-ProRule annotationAdd BLAST113
Domaini583 – 624EGF-like 1; calcium-bindingPROSITE-ProRule annotationAdd BLAST42
Domaini627 – 739CUB 3PROSITE-ProRule annotationAdd BLAST113
Domaini739 – 779EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd BLAST41
Domaini783 – 895CUB 4PROSITE-ProRule annotationAdd BLAST113
Domaini896 – 1012CUB 5PROSITE-ProRule annotationAdd BLAST117

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni157 – 357MetalloproteaseBy similarityAdd BLAST201

Sequence similaritiesi

Belongs to the peptidase M12A family.Curated
Contains 5 CUB domains.PROSITE-ProRule annotation
Contains 2 EGF-like domains.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiKOG3714. Eukaryota.
ENOG410ZPX7. LUCA.
GeneTreeiENSGT00760000119018.
HOGENOMiHOG000236339.
HOVERGENiHBG004859.
InParanoidiO57460.
KOiK09608.
OMAiEEDECSK.
OrthoDBiEOG091G009U.
PhylomeDBiO57460.
TreeFamiTF314351.

Family and domain databases

CDDicd00041. CUB. 5 hits.
Gene3Di2.60.120.290. 5 hits.
3.40.390.10. 1 hit.
InterProiIPR015446. BMP_1/tolloid-like.
IPR000859. CUB_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR024079. MetalloPept_cat_dom.
IPR001506. Peptidase_M12A.
IPR006026. Peptidase_Metallo.
[Graphical view]
PfamiPF01400. Astacin. 1 hit.
PF00431. CUB. 5 hits.
[Graphical view]
PIRSFiPIRSF001199. BMP_1/tolloid-like. 1 hit.
PRINTSiPR00480. ASTACIN.
SMARTiSM00042. CUB. 5 hits.
SM00181. EGF. 2 hits.
SM00179. EGF_CA. 2 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 5 hits.
PROSITEiPS00010. ASX_HYDROXYL. 2 hits.
PS01180. CUB. 5 hits.
PS01186. EGF_2. 2 hits.
PS50026. EGF_3. 2 hits.
PS01187. EGF_CA. 2 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O57460-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYLYSALTS KMNWIALLLA GLTFCCKVSV HSCLDYDDSY DYYEEEKTET
60 70 80 90 100
IDYKDPCKAA VFWGDIALDD EDLKMFHIDG TIDLKQQTHG RQGHTSGGLG
110 120 130 140 150
EHVPTKKRGS LYLLLDRIRR LGFESWPVNS SKDVSSIKTG IRRVNSARNV
160 170 180 190 200
KSRVPRAATS RAEKIWPGGV IPYVIGGNFT GSQRAMLKQA MRHWEKQTCV
210 220 230 240 250
TFIEKTDEES YIVFTYRPCG CCSYVGRRGN GPQAISIGKN CDKFGIVVHE
260 270 280 290 300
LGHVIGFWHE HTRPDRDDHV TIIRDNIQPG QEYNFIKMEP GDVNSLGEPY
310 320 330 340 350
DFDSIMHYAR NTFSRGMFLD TILPSRDENG VRPAIGQRTR LSKGDISQAK
360 370 380 390 400
KLYRCPACGE TLQDSVGNFS SPGYPNGYPS YTHCVWRISV TPGEKIVLNF
410 420 430 440 450
TTMDLYKSSL CWYDYIEVRD GYWRKAPLLG RFCGDKIPEV LVSTDSRMWI
460 470 480 490 500
EFRSSSNWVG KGFAAVYEAI CGGEISKDSG QIQSPNYPDD YRPSKECVWR
510 520 530 540 550
ITVSEGYSVG LSFQVFEIER HDSCAYDYLE VRDGLSENSP LIGRFCGYDK
560 570 580 590 600
PEDIRSTSNN LWMKFVSDGT VNKAGFAANF FKEEDECLKP DNGGCEQRCV
610 620 630 640 650
NTLGSFKCAC DPGYELAPDK KSCEAACGGL LTKLNGTITT PGWPKEYPPN
660 670 680 690 700
KNCVWQVVAP TQYRISMQFE AFELEGNEVC KYDYVEVRSG LSSDSKLHGK
710 720 730 740 750
YCGTEVPEVI TSQYNNMRIE FKSDNTVSKK GFKAHFFSDK DECSKDNGGC
760 770 780 790 800
QHECINTIGS YVCQCRNGFI LHENKHDCKE AECEHKIHST TGTISSPNWP
810 820 830 840 850
DKYPSRKECT WDITATPGHR VKISFNEFEI EQHQECAYDH LEAFDGDSDK
860 870 880 890 900
TPILSRLCGN KIPEPLISTG NKMYLRFISD ASVQRKGFQA THSTECGGRL
910 920 930 940 950
KAEARQKNLY SHAQFGDNNY PGHTDCEWLI VAESGYGIEL TFTTFEVEEE
960 970 980 990 1000
ADCGYDYIEL YDGYDTGAHK IGRFCGSGPR EELYSAGDAV LIHFHSDDTI
1010 1020
SKKGFHIRYT STKFQEALHT RK
Length:1,022
Mass (Da):115,536
Last modified:June 1, 1998 - v1
Checksum:iA68CA1D0E41793F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF027596 mRNA. Translation: AAC60304.1.
RefSeqiNP_571085.1. NM_131010.1.
UniGeneiDr.75803.

Genome annotation databases

EnsembliENSDART00000054472; ENSDARP00000054471; ENSDARG00000037429.
GeneIDi474335.
KEGGidre:474335.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF027596 mRNA. Translation: AAC60304.1.
RefSeqiNP_571085.1. NM_131010.1.
UniGeneiDr.75803.

3D structure databases

ProteinModelPortaliO57460.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000054471.

Proteomic databases

PaxDbiO57460.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000054472; ENSDARP00000054471; ENSDARG00000037429.
GeneIDi474335.
KEGGidre:474335.

Organism-specific databases

CTDi7092.
ZFINiZDB-GENE-041020-1. tll1.

Phylogenomic databases

eggNOGiKOG3714. Eukaryota.
ENOG410ZPX7. LUCA.
GeneTreeiENSGT00760000119018.
HOGENOMiHOG000236339.
HOVERGENiHBG004859.
InParanoidiO57460.
KOiK09608.
OMAiEEDECSK.
OrthoDBiEOG091G009U.
PhylomeDBiO57460.
TreeFamiTF314351.

Enzyme and pathway databases

ReactomeiR-DRE-1474228. Degradation of the extracellular matrix.
R-DRE-1650814. Collagen biosynthesis and modifying enzymes.
R-DRE-2214320. Anchoring fibril formation.
R-DRE-2243919. Crosslinking of collagen fibrils.

Miscellaneous databases

PROiO57460.

Gene expression databases

BgeeiENSDARG00000037429.
ExpressionAtlasiO57460. differential.

Family and domain databases

CDDicd00041. CUB. 5 hits.
Gene3Di2.60.120.290. 5 hits.
3.40.390.10. 1 hit.
InterProiIPR015446. BMP_1/tolloid-like.
IPR000859. CUB_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR024079. MetalloPept_cat_dom.
IPR001506. Peptidase_M12A.
IPR006026. Peptidase_Metallo.
[Graphical view]
PfamiPF01400. Astacin. 1 hit.
PF00431. CUB. 5 hits.
[Graphical view]
PIRSFiPIRSF001199. BMP_1/tolloid-like. 1 hit.
PRINTSiPR00480. ASTACIN.
SMARTiSM00042. CUB. 5 hits.
SM00181. EGF. 2 hits.
SM00179. EGF_CA. 2 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 5 hits.
PROSITEiPS00010. ASX_HYDROXYL. 2 hits.
PS01180. CUB. 5 hits.
PS01186. EGF_2. 2 hits.
PS50026. EGF_3. 2 hits.
PS01187. EGF_CA. 2 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTLL1_DANRE
AccessioniPrimary (citable) accession number: O57460
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: June 1, 1998
Last modified: November 30, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.