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Protein

Ubiquitin carboxyl-terminal hydrolase 2

Gene

USP2

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Hydrolase that deubiquitinates polyubiquitinated target proteins such as MDM2, MDM4 and CCND1. Possesses both ubiquitin-specific peptidase and isopeptidase activities. May play a role in the regulation of the circadian clock.By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei28 – 281NucleophilePROSITE-ProRule annotation
Metal bindingi177 – 1771ZincBy similarity
Metal bindingi180 – 1801ZincBy similarity
Metal bindingi228 – 2281ZincBy similarity
Metal bindingi231 – 2311ZincBy similarity
Active sitei309 – 3091Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ubiquitin-specific protease activity Source: Roslin

GO - Biological processi

  1. protein deubiquitination Source: Roslin
  2. rhythmic process Source: UniProtKB-KW
  3. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Biological rhythms, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiC19.013.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 2 (EC:3.4.19.12)
Alternative name(s):
41 kDa ubiquitin-specific protease
Deubiquitinating enzyme 2
Ubiquitin thioesterase 2
Ubiquitin-specific-processing protease 2
Gene namesi
Name:USP2
Synonyms:UBP41
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Unplaced

Subcellular locationi

Cytoplasm By similarity. Cytoplasmperinuclear region By similarity

GO - Cellular componenti

  1. perinuclear region of cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 357357Ubiquitin carboxyl-terminal hydrolase 2PRO_0000080618Add
BLAST

Proteomic databases

PaxDbiO57429.

Interactioni

Subunit structurei

Homooligomer.By similarity

Protein-protein interaction databases

STRINGi9031.ENSGALP00000010901.

Structurei

3D structure databases

ProteinModelPortaliO57429.
SMRiO57429. Positions 16-352.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 351333USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family. USP2 subfamily.Curated
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiCOG5560.
HOVERGENiHBG011164.
InParanoidiO57429.
PhylomeDBiO57429.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O57429-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAARMAPTPR SSKVVQGLTG LRNLGNTCFM NSILQCLSNT KELRDYCLQN
60 70 80 90 100
QYLRDLNNNS RMRTALMSEF AKLIQLLWTS SPNDSVSPSE FKTQIQRYAP
110 120 130 140 150
RFVGYNQQDA QEFLRFLLDG LHGEVNRVLV RPRANADTLD HLPDDEKSRQ
160 170 180 190 200
MWRRYQERED SRVSDLFVGQ LKSSLTCSEC GYCSTAFDPF WDLSLPIPKK
210 220 230 240 250
GYGEVTLMDC LRLFTKEDVL DGDEKPTCCR CKARTRCTKK FSIQKFPKIL
260 270 280 290 300
VLHLKRFSEA RIRASKLTTF VNFPLKDLDL REFASQSCNH AVYNLYAVSN
310 320 330 340 350
HSGTTMGGHY TAYCKSPISS EWHSFNDSRV TPMSSSHVRS SDAYLLFYEL

ASPSSRM
Length:357
Mass (Da):40,932
Last modified:June 1, 1998 - v1
Checksum:iEC39E6454937C455
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF016107 mRNA. Translation: AAC13729.1.
UniGeneiGga.454.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF016107 mRNA. Translation: AAC13729.1.
UniGeneiGga.454.

3D structure databases

ProteinModelPortaliO57429.
SMRiO57429. Positions 16-352.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000010901.

Protein family/group databases

MEROPSiC19.013.

Proteomic databases

PaxDbiO57429.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiCOG5560.
HOVERGENiHBG011164.
InParanoidiO57429.
PhylomeDBiO57429.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning of a novel ubiquitin-specific protease, UBP41, with isopeptidase activity in chick skeletal muscle."
    Baek S., Choi K.S., Yoo Y.J., Cho J.M., Baker R.T., Tanaka K., Chung C.H.
    J. Biol. Chem. 272:25560-25565(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Skeletal muscle.

Entry informationi

Entry nameiUBP2_CHICK
AccessioniPrimary (citable) accession number: O57429
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: June 1, 1998
Last modified: March 4, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.