O57429 (UBP2_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 2
EC=3.4.19.12

Alternative name(s):
41 kDa ubiquitin-specific protease
Deubiquitinating enzyme 2
Ubiquitin thioesterase 2
Ubiquitin-specific-processing protease 2

Gene names

Name:	USP2
Synonyms:	UBP41

Organism

Gallus gallus (Chicken) [Reference proteome]

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	357 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolase that deubiquitinates polyubiquitinated target proteins such as MDM2, MDM4 and CCND1. Possesses both ubiquitin-specific peptidase and isopeptidase activities By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Cofactor	Binds 1 zinc ion By similarity.
Subunit structure	Homooligomer By similarity.
Subcellular location	Cytoplasm By similarity. Cytoplasm › perinuclear region By similarity.
Sequence similarities	Belongs to the peptidase C19 family. USP2 subfamily.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein deubiquitination Inferred from direct assay Ref.1. Source: Roslin ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro ubiquitin-specific protease activity Inferred from direct assay Ref.1. Source: Roslin
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 357	357	Ubiquitin carboxyl-terminal hydrolase 2		PRO_0000080618
Sites
	Active site	28	1	Nucleophile By similarity
	Active site	309	1	Proton acceptor By similarity
	Metal binding	177	1	Zinc By similarity
	Metal binding	180	1	Zinc By similarity
	Metal binding	228	1	Zinc By similarity
	Metal binding	231	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	O57429 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: EC39E6454937C455	FASTA	357	40,932
	10 20 30 40 50 60 MAARMAPTPR SSKVVQGLTG LRNLGNTCFM NSILQCLSNT KELRDYCLQN QYLRDLNNNS 70 80 90 100 110 120 RMRTALMSEF AKLIQLLWTS SPNDSVSPSE FKTQIQRYAP RFVGYNQQDA QEFLRFLLDG 130 140 150 160 170 180 LHGEVNRVLV RPRANADTLD HLPDDEKSRQ MWRRYQERED SRVSDLFVGQ LKSSLTCSEC 190 200 210 220 230 240 GYCSTAFDPF WDLSLPIPKK GYGEVTLMDC LRLFTKEDVL DGDEKPTCCR CKARTRCTKK 250 260 270 280 290 300 FSIQKFPKIL VLHLKRFSEA RIRASKLTTF VNFPLKDLDL REFASQSCNH AVYNLYAVSN 310 320 330 340 350 HSGTTMGGHY TAYCKSPISS EWHSFNDSRV TPMSSSHVRS SDAYLLFYEL ASPSSRM

References

[1]

"Molecular cloning of a novel ubiquitin-specific protease, UBP41, with isopeptidase activity in chick skeletal muscle." Baek S., Choi K.S., Yoo Y.J., Cho J.M., Baker R.T., Tanaka K., Chung C.H. J. Biol. Chem. 272:25560-25565(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Skeletal muscle.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF016107 mRNA. Translation: AAC13729.1.
IPI	IPI00577228.
UniGene	Gga.454.
3D structure databases
ProteinModelPortal	O57429.
SMR	O57429. Positions 16-352.
ModBase	Search...
Protein-protein interaction databases
STRING	9031.ENSGALP00000010901.
Protein family/group databases
MEROPS	C19.013.
Proteomic databases
PaxDb	O57429.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	COG5560.
HOVERGEN	HBG011164.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. [Graphical view]
Pfam	PF00443. UCH. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBP2_CHICK

Accession

Primary (citable) accession number: O57429

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	June 1, 1998
Last modified:	April 3, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program