Reviewed, UniProtKB/Swiss-Prot O57427 (HAS2_XENLA)

Names and origin

Protein names

Recommended name:
    Hyaluronan synthase 2
    EC=2.4.1.212
Alternative name(s):
    Hyaluronate synthase 2
    Hyaluronic acid synthase 2
      Short name=HA synthase 2
      Short name=xHAS2

Gene names

Name:

has2

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	551 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays a role in hyaluronan/hyaluronic acid (HA) synthesis By similarity.
Catalytic activity	UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan) = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan). UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan) = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan).
Cofactor	Magnesium.
Pathway	Glycan biosynthesis; hyaluronan biosynthesis.
Subcellular location	Membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the nodC/HAS family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hyaluronan synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 551	551	Hyaluronan synthase 2		PRO_0000197177
Regions
	Topological domain	1 – 11	11	Cytoplasmic Potential
	Transmembrane	12 – 32	21	1 Potential
	Topological domain	33 – 45	13	Extracellular Potential
	Transmembrane	46 – 66	21	2 Potential
	Topological domain	67 – 374	308	Cytoplasmic Potential
	Transmembrane	375 – 395	21	3 Potential
	Topological domain	396 – 402	7	Extracellular Potential
	Transmembrane	403 – 423	21	4 Potential
	Topological domain	424 – 429	6	Cytoplasmic Potential
	Transmembrane	430 – 450	21	5 Potential
	Topological domain	451 – 470	20	Extracellular Potential
	Transmembrane	471 – 491	21	6 Potential
	Topological domain	492 – 509	18	Cytoplasmic Potential
	Transmembrane	510 – 530	21	7 Potential
	Topological domain	531 – 551	21	Extracellular Potential

Sequences

	Sequence		Length	Mass (Da)
	O57427-1 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: AAD902B3B4802A7D	FASTA	551	63,686
	10 20 30 40 50 60 MHCERFICIL RIIGTTLFGV SLLLGISAAY IVGYQFIQTD NYYFSFGLYG AILALHLIIQ 70 80 90 100 110 120 SLFAFLEHRK MKRSLETPIK LNKSVALCIA AYQEDEDYLR KCLLSVKRLT YPGMKVIMVI 130 140 150 160 170 180 DGNSDDDLYM MNIFREIMGN DSCATYVWKN NFHMKGPNET DETHRESMQH VTQMVLSNRN 190 200 210 220 230 240 VCIMQKWNGK REVMYTAFKA LGRSVDYVQV CDSDTVLDPA SSVEMVKVLE EDIMVGGVGG 250 260 270 280 290 300 DVQILNKYDS WISFLSSVRY WMAFNIERAC QSYFGCVQCI SGPLGMYRNS LLHEFIEDWY 310 320 330 340 350 360 NQEFLGSQCS FGDDRHLTNR VLSLGYATKY TARSKCLTET PTEYLRWLNQ QTRWSKSYFR 370 380 390 400 410 420 EWLYNSLWFH KHHLWMTYEA VITGFFPFFL IATVIQLFYR GRIWNILLFL LTVQLVGLIK 430 440 450 460 470 480 SSFASALRGN IVMVFMSFYS VLYMSSLLPA KMFAIATINK AGWGTSGRKT IVVNFIGLIP 490 500 510 520 530 540 ITVWFTILLG GVCYTIWRET KKPFSESEKI VLAVGAILYA CYWVMLLTMY VSLVMKCGRR 550 RKEPQHDLVL A

References

[1]	"Synthesis of hyaluronan of distinctly different chain length is regulated by the differential expression of Xhas1 and 2 during early development of Xenopus laevis." Koeprunner M., Muellegger J., Lepperdinger G. Mech. Dev. 90:275-278(2000) [PubMed: 10640711] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Characterization and molecular evolution of a vertebrate hyaluronan synthase gene family." Spicer A.P., McDonald J.A. J. Biol. Chem. 273:1923-1932(1998) [PubMed: 9442026] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 272-461.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF168465 mRNA. Translation: AAD45813.1. AF015779 Genomic DNA. Translation: AAB94540.1.
RefSeq	NP_001083837.1.
UniGene	Xl.608
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Genome annotation databases
GeneID	399146.
KEGG	xla:399146.
Organism-specific databases
CTD	399146.
Xenbase	XB-FEAT-5775580. has2.
Phylogenomic databases
HOVERGEN	O57427.
Enzyme and pathway databases
BRENDA	2.4.1.212. 648.
Family and domain databases
InterPro	IPR001173. Glyco_trans_2. [Graphical view]
Pfam	PF00535. Glycos_transf_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HAS2_XENLA

Accession

Primary (citable) accession number: O57427

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	October 13, 2009
This is version 50 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project