Reviewed, UniProtKB/Swiss-Prot O57426 (HAS3_XENLA)

Names and origin

Protein names

Recommended name:
    Hyaluronan synthase 3
    EC=2.4.1.212
Alternative name(s):
    Hyaluronate synthase 3
    Hyaluronic acid synthase 3
      Short name=HA synthase 3
      Short name=xHAS3

Gene names

Name:

has3

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	190 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays a role in hyaluronan/hyaluronic acid (HA) synthesis By similarity.
Catalytic activity	UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan) = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan). UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan) = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan).
Cofactor	Magnesium.
Pathway	Glycan biosynthesis; hyaluronan biosynthesis.
Subcellular location	Membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the nodC/HAS family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hyaluronan synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›190	›190	Hyaluronan synthase 3		PRO_0000197181
Regions
	Topological domain	‹1 – 103	›103	Cytoplasmic Potential
	Transmembrane	104 – 124	21	3 Potential
	Topological domain	125 – 134	10	Extracellular Potential
	Transmembrane	135 – 155	21	4 Potential
	Topological domain	156 – 166	11	Cytoplasmic Potential
	Transmembrane	167 – 187	21	5 Potential
	Topological domain	188 – ›190	›3	Extracellular Potential
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	190	1

Sequences

	Sequence		Length	Mass (Da)
	O57426-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: EFD418FAE9B6F867	FASTA	190	22,609
	10 20 30 40 50 60 SYFGCVQCIS GPLGMYRNSL LQYFLEDWYH QTFLGQKCSF GDDRHLTNRV LSMGFRTKYT 70 80 90 100 110 120 ARSRCLTETP TRYLRWLNQQ TRWSKSYFRE WLYNALWFHK HHLWMTYESV VTGFFPFFLV 130 140 150 160 170 180 ATVVQLFYRG RVWNILLFLL TVQLVGILKA TYACILRGNA EMIFMSLYSL LYMTSLLPAK 190 IFAVITINKS

References

[1]	"Characterization and molecular evolution of a vertebrate hyaluronan synthase gene family." Spicer A.P., McDonald J.A. J. Biol. Chem. 273:1923-1932(1998) [PubMed: 9442026] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF015778 Genomic DNA. Translation: AAB94539.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Phylogenomic databases
HOVERGEN	O57426.
Enzyme and pathway databases
BRENDA	2.4.1.212. 648.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

HAS3_XENLA

Accession

Primary (citable) accession number: O57426

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	June 1, 1998
Last modified:	October 13, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project