O57424 (HAS2_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hyaluronan synthase 2
EC=2.4.1.212

Alternative name(s):
Hyaluronate synthase 2
Hyaluronic acid synthase 2
Short name=CHAS2
Short name=HA synthase 2

Gene names

Name:

HAS2

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	552 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Plays a role in hyaluronan/hyaluronic acid (HA) synthesis By similarity.
Catalytic activity	UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan) = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan). UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan) = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan).
Cofactor	Magnesium.
Pathway	Glycan biosynthesis; hyaluronan biosynthesis.
Subcellular location	Membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the nodC/HAS family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	hyaluronan synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 552	552	Hyaluronan synthase 2		PRO_0000197176
Regions
	Topological domain	1 – 11	11	Cytoplasmic Potential
	Transmembrane	12 – 32	21	Helical; Name=1; Potential
	Topological domain	33 – 45	13	Extracellular Potential
	Transmembrane	46 – 66	21	Helical; Name=2; Potential
	Topological domain	67 – 374	308	Cytoplasmic Potential
	Transmembrane	375 – 395	21	Helical; Name=3; Potential
	Topological domain	396 – 402	7	Extracellular Potential
	Transmembrane	403 – 423	21	Helical; Name=4; Potential
	Topological domain	424 – 429	6	Cytoplasmic Potential
	Transmembrane	430 – 450	21	Helical; Name=5; Potential
	Topological domain	451 – 475	25	Extracellular Potential
	Transmembrane	476 – 496	21	Helical; Name=6; Potential
	Topological domain	497 – 510	14	Cytoplasmic Potential
	Transmembrane	511 – 531	21	Helical; Name=7; Potential
	Topological domain	532 – 552	21	Extracellular Potential

Sequences

	Sequence		Length	Mass (Da)
	O57424 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 4AF7819347E71F7A	FASTA	552	63,745
	10 20 30 40 50 60 MYCERFICIL RILGTTLFGV SLLLGITAAY IVGYQFIQTD NYYFSFGLYG AILASHLIIQ 70 80 90 100 110 120 SLFAYLEHRK MKRSLETPIK LNKTVALCIA AYQEDPDYLR KCLLSVKRLT YPGIKVVMVI 130 140 150 160 170 180 DGNSEDDVYM MDIFTEIMGR DKSATYIWSN NFHDKGPGET EESHRESMQH VSQLVLSNKS 190 200 210 220 230 240 VCIMQKWGGK REVMYTAFKA LGEAWNYVQV CDSDTMLDPA SSVEMVKVLE EDPMVGGVGG 250 260 270 280 290 300 DVQILNKYDS WISFLSSVRY WMAFNIERAC QSYFGCVQCI SGPLGMYRNS LLHEFVEDWY 310 320 330 340 350 360 NQEFMGSQCS FGDDRHLTNR VLSLGYATKY TARSKCLTET PIEYLRWLNQ QTRWSKSYFR 370 380 390 400 410 420 EWLYNAMWFH KHHLWMTYEA VITGFFPFFL IATVIQLFYR GKIWNILLFL LTVQLVGLIK 430 440 450 460 470 480 SSFASFLRGN IVMVFMSLYS VLYMSSLLPA KMFAIATINK AGWGTSGRKT IVVNFIGLIP 490 500 510 520 530 540 VSIWFTILLG RVIFTIYKES KKPFSESKTT VLVIGTILYA CYWVLLLTLY LVLITKCGRR 550 KKEQHYDMVL DV

References

[1]	"Gallus gallus hyaluronan synthase 2 (HAS2) gene." Huang L., Toole B.P. Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Characterization and molecular evolution of a vertebrate hyaluronan synthase gene family." Spicer A.P., McDonald J.A. J. Biol. Chem. 273:1923-1932(1998) [PubMed: 9442026] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 272-461. Strain: White leghorn.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF106940 mRNA. Translation: AAF14347.1. AF015776 Genomic DNA. Translation: AAB94537.1.
IPI	IPI00581970.
RefSeq	NP_990137.1. NM_204806.1.
UniGene	Gga.329.
3D structure databases
ProteinModelPortal	O57424.
ModBase	Search...
Protein-protein interaction databases
STRING	O57424.
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	395594.
KEGG	gga:395594.
Organism-specific databases
CTD	3037.
Phylogenomic databases
eggNOG	veNOG13751.
GeneTree	ENSGT00390000010337.
HOGENOM	HBG716008.
HOVERGEN	HBG000189.
InParanoid	O57424.
OrthoDB	EOG4KH2TP.
Family and domain databases
InterPro	IPR004835. Chitin_synth_fng. [Graphical view]
KO	K00752.
Pfam	PF03142. Chitin_synth_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HAS2_CHICK

Accession

Primary (citable) accession number: O57424

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	November 16, 2011
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program