Reviewed, UniProtKB/Swiss-Prot O57391 (ENOG_CHICK)

Names and origin

Protein names

Recommended name:
    Gamma-enolase
    EC=4.2.1.11
Alternative name(s):
    2-phospho-D-glycerate hydro-lyase
    Neural enolase
      Short name=NSE

Gene names

Name:

ENO2

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	434 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	2-phospho-D-glycerate = phosphoenolpyruvate + H2O.
Cofactor	Magnesium. Required for catalysis and for stabilizing the dimer By similarity.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Tissue specificity	Expressed in the brain and, to much less but significant extents, in the pituitary and adrenal glands.
Sequence similarities	Belongs to the enolase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Lyase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	phosphopyruvate hydratase complex Inferred from electronic annotation. Source: InterPro
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphopyruvate hydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 434	434	Gamma-enolase		PRO_0000134115
Regions
	Region	370 – 373	4	Substrate binding By similarity
Sites
	Active site	210	1	Proton donor By similarity
	Active site	343	1	Proton acceptor By similarity
	Metal binding	245	1	Magnesium By similarity
	Metal binding	293	1	Magnesium By similarity
	Metal binding	318	1	Magnesium By similarity
	Binding site	158	1	Substrate By similarity
	Binding site	167	1	Substrate By similarity
	Binding site	293	1	Substrate By similarity
	Binding site	318	1	Substrate By similarity
	Binding site	394	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O57391-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 359E32F78BACCE07	FASTA	434	47,308
	10 20 30 40 50 60 MAVERIHARE ILDSRGNPTV EVDLYTHKGM FRAAVPSGAS TGIYEALELR DNDKSRFLGK 70 80 90 100 110 120 GVLQAVDHIN STVAPAIVGS GLSVVDQEKI DNLMLEMDGT ENKSKFGANA ILGVSLAVCK 130 140 150 160 170 180 AGAAEKDVPL YRHIADLAGN SDLILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAESFRD 190 200 210 220 230 240 AMRIGAEVYH NLKSVIKEKY GKDATNVGDE GGFAPNILEN SEALELLKEA IDKAGYTDKI 250 260 270 280 290 300 VIGMDVAASE FYRDGKYDLD FKSPDDPSRY ISADELGDLY QSFVRAYPVL SIEDPFDQDD 310 320 330 340 350 360 WEAWSKFTAN VGIQIVGDDL TVTNPKRIER AVEEKACNCL LLKVNQIGSV TEAIQACKLA 370 380 390 400 410 420 QENGWGVMVS HRSGETEDTF IADLVVALCT GQIKTGAPCR SERLAKYNQL MRIEEELGDE 430 ARFAGHNFRN PSVL

References

[1]	"cDNA cloning and characterization of neuron-specific enolase from chicken." Tanaka M., Taniguchi T., Ohkubo T., Nakashima K. Biochim. Biophys. Acta 1395:28-33(1998) [PubMed: 9434147] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB004291 mRNA. Translation: BAA24680.1.
IPI	IPI00592520.
RefSeq	NP_990207.1.
UniGene	Gga.4132
3D structure databases
HSSP	HSSP built from PDB template 1PDZ based on UniProtKB P56252.
SMR	O57391. Positions 2-434.
ModBase	Search...
Protein-protein interaction databases
STRING	O57391.
Proteomic databases
PRIDE	O57391.
Genome annotation databases
Ensembl	ENSGALT00000023444; ENSGALP00000023398; ENSGALG00000014528; Gallus gallus. [Genome view]
GeneID	395689.
KEGG	gga:395689.
Organism-specific databases
CTD	395689.
Phylogenomic databases
HOGENOM	O57391.
HOVERGEN	O57391.
Enzyme and pathway databases
BRENDA	4.2.1.11. 4.
Family and domain databases
InterPro	IPR000941. Enolase. [Graphical view]
PANTHER	PTHR11902. Enolase. 1 hit.
Pfam	PF00113. Enolase_C. 1 hit. PF03952. Enolase_N. 1 hit. [Graphical view]
PIRSF	PIRSF001400. Enolase. 1 hit.
PRINTS	PR00148. ENOLASE.
ProDom	PD000902. Enolase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01060. eno. 1 hit.
PROSITE	PS00164. ENOLASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ENOG_CHICK

Accession

Primary (citable) accession number: O57391

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	June 1, 1998
Last modified:	September 1, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)