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Protein

Tolloid-like protein 2

Gene

tll2

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Protease which specifically processes prolysyl oxidase and maybe also chordin. Required for the embryonic development. Predominant protease, which in the development, influences dorsal-ventral patterning and skeletogenesis. In embryos, inhibits the development of dorsoanterior structures and ventralizes activin-induced dorsal mesoderm in animal caps.1 Publication

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi245Zinc; catalyticPROSITE-ProRule annotation1
Active sitei246PROSITE-ProRule annotation1
Metal bindingi249Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi255Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Metalloprotease, Protease

Keywords - Biological processi

Differentiation

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Protein family/group databases

MEROPSiM12.015.

Names & Taxonomyi

Protein namesi
Recommended name:
Tolloid-like protein 2 (EC:3.4.24.-)
Alternative name(s):
Metalloprotease xolloid
Xenopus tolloid
Gene namesi
Name:tll2
Synonyms:xld
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-478934. tll2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Sequence analysisAdd BLAST31
PropeptideiPRO_000004604032 – 152By similarityAdd BLAST121
ChainiPRO_0000046041153 – 1019Tolloid-like protein 2Add BLAST867

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi174N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi354 ↔ 380By similarity
Glycosylationi364N-linked (GlcNAc...)Sequence analysis1
Glycosylationi395N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi407 ↔ 429By similarity
Disulfide bondi467 ↔ 493By similarity
Disulfide bondi520 ↔ 542By similarity
Disulfide bondi583 ↔ 595By similarity
Disulfide bondi591 ↔ 604By similarity
Disulfide bondi606 ↔ 619By similarity
Disulfide bondi623 ↔ 649By similarity
Glycosylationi631N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi676 ↔ 698By similarity
Disulfide bondi739 ↔ 750By similarity
Disulfide bondi746 ↔ 759By similarity
Disulfide bondi761 ↔ 774By similarity
Disulfide bondi779 ↔ 805By similarity
Disulfide bondi832 ↔ 854By similarity
Disulfide bondi892 ↔ 922By similarity
Disulfide bondi949 ↔ 971By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PRIDEiO57382.

Expressioni

Developmental stagei

Initial uniform expression becomes localized to two posterior ectodermal patches flanking the neural plate and later to the inner ectoderm of the developing tailbud. Also expressed in dorsal regions of the brain during tailbud stages and is especially abundant in the ventricular layer of the dorsal hindbrain caudal to the otic vesicle.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO57382.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini354 – 466CUB 1PROSITE-ProRule annotationAdd BLAST113
Domaini467 – 579CUB 2PROSITE-ProRule annotationAdd BLAST113
Domaini579 – 620EGF-like 1; calcium-bindingPROSITE-ProRule annotationAdd BLAST42
Domaini623 – 735CUB 3PROSITE-ProRule annotationAdd BLAST113
Domaini735 – 775EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd BLAST41
Domaini779 – 891CUB 4PROSITE-ProRule annotationAdd BLAST113
Domaini892 – 1008CUB 5PROSITE-ProRule annotationAdd BLAST117

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni153 – 353MetalloproteaseBy similarityAdd BLAST201

Sequence similaritiesi

Belongs to the peptidase M12A family.Curated
Contains 5 CUB domains.PROSITE-ProRule annotation
Contains 2 EGF-like domains.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

HOVERGENiHBG004859.
KOiK13047.

Family and domain databases

CDDicd00041. CUB. 5 hits.
Gene3Di2.60.120.290. 5 hits.
3.40.390.10. 1 hit.
InterProiIPR015446. BMP_1/tolloid-like.
IPR000859. CUB_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR024079. MetalloPept_cat_dom.
IPR001506. Peptidase_M12A.
IPR006026. Peptidase_Metallo.
[Graphical view]
PfamiPF01400. Astacin. 1 hit.
PF00431. CUB. 5 hits.
PF00008. EGF. 1 hit.
[Graphical view]
PIRSFiPIRSF001199. BMP_1/tolloid-like. 1 hit.
PRINTSiPR00480. ASTACIN.
SMARTiSM00042. CUB. 5 hits.
SM00181. EGF. 2 hits.
SM00179. EGF_CA. 2 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 5 hits.
PROSITEiPS01180. CUB. 5 hits.
PS01186. EGF_2. 2 hits.
PS50026. EGF_3. 2 hits.
PS01187. EGF_CA. 2 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O57382-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSCGSPQVMM TLWTLTCVGL ILLGAIRLSL GLDYDLESFD YLMEDNPEEF
60 70 80 90 100
DYKDPCKAAA YWGDIALDED DLKWIFKNKS NDLRNTRHNQ THPTTDNFSE
110 120 130 140 150
KLGTGSQNET SSNLNSKKVK KGSRLKLLIA EKAATETNST FQVQTSNDRV
160 170 180 190 200
RRAATSRTER IWPGGIIPYA IAGNFTGTQR AIFKQAMRHW KKHTCVTFVE
210 220 230 240 250
RTDEESFIVF TYRPCGCCSY VGRRGGGPQA ISIGKNCDKF GIVVHELGHV
260 270 280 290 300
VGFWHEHTRP DRDEHVSIIR ENIQPGQEYN FLKMEPGEVS SLGETYDFDS
310 320 330 340 350
IMHYARNTFS RGVFLDTILP RRIDTSVRPT IGQRIRLSQG DIAQAKKLYK
360 370 380 390 400
CPACGETLQD SSGNFSAPGY PSGYPSYTHC IWRISVTPGE KIILNFTTMD
410 420 430 440 450
LFKSRLCWYD YIEIRDGYWR KAALLGRLCG DKLPDPIISS DSKLWIEFRS
460 470 480 490 500
SSNILGKGFF AAYEAICGGD IKKDSGQIQS PNYPDDYRPA KECIWKITVS
510 520 530 540 550
EGFLVGLSFQ AFEIERHDNC AYDYLEVRDG FSEDHALIGR FCGYEKPEDI
560 570 580 590 600
KSTSNKLWIK FASDGSINKA GFSANFFKEM DECSRPDNGG CSQRCVNTLG
610 620 630 640 650
SYKCVCEPGF ELTADKKSCE AACGGFITQL NGTITSPGWP KEYPTNKNCV
660 670 680 690 700
WQVVAPAQYR ISLQFEVFEL EGNDVCKYDY LEIRSGLSSE SKLHGKFCGP
710 720 730 740 750
EKPEVITSQG NTVRIEFKSD NTVSKKGFKA NFFSDKDECS KDNGGCQHDC
760 770 780 790 800
VNTFGSYICQ CKNGFILHEN GHDCKEAGCE QKLLNAEGTI SSPNWPEKYP
810 820 830 840 850
SRKECTWDIS VTAGHRVKLV FTDFEIEQHQ ECAYDHLELY DGPNGKAAIL
860 870 880 890 900
GRFCGSKEPS PVVASTNNMF LRFYSDASVQ RKGFQAKYSP ECGGRLKAEI
910 920 930 940 950
QTNDIYSHAQ FGDNNYPVQS NCEWVIVAED GYGVELIFQT FEIEEESDCG
960 970 980 990 1000
YDYMEVYDGY DSTAPRLGRY CGSGPPEEMY SAGDSIMIRF HTDDTINKKG
1010
FHGQYTSTKF QDALHMRRK
Length:1,019
Mass (Da):114,892
Last modified:June 1, 1998 - v1
Checksum:i50CCE8B7E3B42C6E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09661 mRNA. Translation: CAA70854.1.
RefSeqiNP_001084377.1. NM_001090908.1.
UniGeneiXl.36.

Genome annotation databases

GeneIDi399469.
KEGGixla:399469.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09661 mRNA. Translation: CAA70854.1.
RefSeqiNP_001084377.1. NM_001090908.1.
UniGeneiXl.36.

3D structure databases

ProteinModelPortaliO57382.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiM12.015.

Proteomic databases

PRIDEiO57382.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi399469.
KEGGixla:399469.

Organism-specific databases

CTDi7093.
XenbaseiXB-GENE-478934. tll2.

Phylogenomic databases

HOVERGENiHBG004859.
KOiK13047.

Family and domain databases

CDDicd00041. CUB. 5 hits.
Gene3Di2.60.120.290. 5 hits.
3.40.390.10. 1 hit.
InterProiIPR015446. BMP_1/tolloid-like.
IPR000859. CUB_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR024079. MetalloPept_cat_dom.
IPR001506. Peptidase_M12A.
IPR006026. Peptidase_Metallo.
[Graphical view]
PfamiPF01400. Astacin. 1 hit.
PF00431. CUB. 5 hits.
PF00008. EGF. 1 hit.
[Graphical view]
PIRSFiPIRSF001199. BMP_1/tolloid-like. 1 hit.
PRINTSiPR00480. ASTACIN.
SMARTiSM00042. CUB. 5 hits.
SM00181. EGF. 2 hits.
SM00179. EGF_CA. 2 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 5 hits.
PROSITEiPS01180. CUB. 5 hits.
PS01186. EGF_2. 2 hits.
PS50026. EGF_3. 2 hits.
PS01187. EGF_CA. 2 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTLL2_XENLA
AccessioniPrimary (citable) accession number: O57382
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: June 1, 1998
Last modified: November 30, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.