O57329 (FZD7_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Frizzled-7
Short name=Fz-7
Short name=cFz-7

Gene names

Name:	FZD7
Synonyms:	FZ7

Organism

Gallus gallus (Chicken) [Reference proteome]

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	567 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
Subcellular location	Membrane; Multi-pass membrane protein By similarity. Cell membrane; Multi-pass membrane protein By similarity.
Tissue specificity	Expressed broadly in cranial ectoderm. Also expressed in the developing somites and in other cranial placodes, including the olfactory, lens, otic placodes (lateral half of the vesicle) and epibranchial placodes. Low level of expression in all the mesoderm derivatives in the limb buds.
Developmental stage	First detected as stage 6 in the forming neural tube and somites, but not in trunk surface ectoderm. By stage 8, expression persists in the cranial ectoderm and is up-regulated in the presomptive olfactory placodes. By stages 11-12, expression declines in the neural tube, but not in the cranial ectoderm; in somites, expressed all along the rostral-caudal axis as well as in presegmental mesenchyme caudal to the developing somites. Lens and otic placode expression first visible at stage 12, strongest at stages 13-16. Detected uniformly in ectoderm and mesenchyme of the limb primordia at stage 17. By stage 18, decrease of ectodermal, otic, lens and olfactory placode expression; expression appears in the epibranchial placodes. By stages 22-30, highest levels in the most distal mesoderm of the autopod, in the ventricular zone of the neural tube from the forebrain to the spinal cord, in the dermomyotomes and the tail buds.
Domain	Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway By similarity. The FZ domain is involved in binding with Wnt ligands By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor Fz/Smo family. Contains 1 FZ (frizzled) domain.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Cell membrane Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Developmental protein G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled receptor signaling pathway coupled to cGMP nucleotide second messenger Inferred from Biological aspect of Ancestor. Source: RefGenome Wnt receptor signaling pathway, calcium modulating pathway Inferred from Biological aspect of Ancestor. Source: RefGenome axonogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome brain development Inferred from Biological aspect of Ancestor. Source: RefGenome canonical Wnt receptor signaling pathway Inferred from Biological aspect of Ancestor. Source: RefGenome embryo development Inferred from Biological aspect of Ancestor. Source: RefGenome epithelial cell differentiation Inferred from Biological aspect of Ancestor. Source: RefGenome gonad development Inferred from Biological aspect of Ancestor. Source: RefGenome vasculature development Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	apical part of cell Inferred from Biological aspect of Ancestor. Source: RefGenome cytoplasm Inferred from Biological aspect of Ancestor. Source: RefGenome integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW neuron projection membrane Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	G-protein coupled receptor activity Inferred from electronic annotation. Source: UniProtKB-KW PDZ domain binding Inferred from Biological aspect of Ancestor. Source: RefGenome Wnt-activated receptor activity Inferred from Biological aspect of Ancestor. Source: RefGenome Wnt-protein binding Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Chain	32 – 567	536	Frizzled-7		PRO_0000012998
Regions
	Topological domain	32 – 250	219	Extracellular Potential
	Transmembrane	251 – 271	21	Helical; Name=1; Potential
	Topological domain	272 – 282	11	Cytoplasmic Potential
	Transmembrane	283 – 303	21	Helical; Name=2; Potential
	Topological domain	304 – 330	27	Extracellular Potential
	Transmembrane	331 – 351	21	Helical; Name=3; Potential
	Topological domain	352 – 373	22	Cytoplasmic Potential
	Transmembrane	374 – 394	21	Helical; Name=4; Potential
	Topological domain	395 – 417	23	Extracellular Potential
	Transmembrane	418 – 438	21	Helical; Name=5; Potential
	Topological domain	439 – 464	26	Cytoplasmic Potential
	Transmembrane	465 – 485	21	Helical; Name=6; Potential
	Topological domain	486 – 521	36	Extracellular Potential
	Transmembrane	522 – 542	21	Helical; Name=7; Potential
	Topological domain	543 – 567	25	Cytoplasmic Potential
	Domain	42 – 161	120	FZ
	Motif	545 – 550	6	Lys-Thr-X-X-X-Trp motif, mediates interaction with the PDZ domain of Dvl family members By similarity
	Motif	565 – 567	3	PDZ-binding
Amino acid modifications
	Glycosylation	61	1	N-linked (GlcNAc...) Potential
	Glycosylation	162	1	N-linked (GlcNAc...) Potential
	Disulfide bond	47 ↔ 108		By similarity
	Disulfide bond	55 ↔ 101		By similarity
	Disulfide bond	92 ↔ 129		By similarity
	Disulfide bond	118 ↔ 158		By similarity
	Disulfide bond	122 ↔ 146		By similarity
Experimental info
	Sequence conflict	34 – 35	2	HE → QD in AAF61097. Ref.2
	Sequence conflict	480	1	V → L in AAF61097. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O57329 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 3EFF0381FC899BC3	FASTA	567	62,811
	10 20 30 40 50 60 MRPAAGEAGA GLRWLGLAAL LAALLGTPCA AAHHEDKAIS VPDHGFCQPI SIPLCTDIAY 70 80 90 100 110 120 NQTILPNLLG HTNQEDAGLE VHQFYPLVKV QCSAELKFFL CSMYAPVCTV LEQAIPPCRS 130 140 150 160 170 180 LCERARQGCE ALMNKFGFQW PERLRCENFP VHGAGEICVG QNTSDAPPGP GGAGGRGATA 190 200 210 220 230 240 QPTAGYLPDL LTPPQPAAGF SFSCPRQLKV PPYLGYRFLG ERDCGAPCEP GRPNGLMYFK 250 260 270 280 290 300 EAEVRFARLW VGVWSVLCCA STLFTVLTYL VDMRRFSYPE RPIIFLSGCY FMVAVAYAAG 310 320 330 340 350 360 FLLEERVVCL ERFSEDGYRT VAQGTKKEGC TILFMILYFF GMASSIWWVI LSLTWFLAAG 370 380 390 400 410 420 MKWGHEAIEA NSQYFHLAAW AVPAVKTITI LAMGQVDGDV LSGVCYVGIY SVDSLRGFVL 430 440 450 460 470 480 APLFVYLFIG TSFLLAGFVS LFRIRTIMKH DGTKTEKLEK LMVRIGVFSV LYTVPATIVV 490 500 510 520 530 540 ACYFYEQAFR STWEKTWLLQ TCKTYAVPCP SHFAPMSPDF TVFMIKYLMT MIVGITTGFW 550 560 IWSGKTLQSW RRFYHRLSTG SKGETAV

References

[1]	"Expression of Wnt and Frizzled genes during chick limb bud development." Kengaku M., Twombly V., Tabin C. Cold Spring Harb. Symp. Quant. Biol. 62:421-429(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Limb bud.
[2]	"Characterization of avian frizzled genes in cranial placode development." Stark M.R., Biggs J.J., Schoenwolf G.C., Rao M.S. Mech. Dev. 93:195-200(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF031831 mRNA. Translation: AAB87969.1. AF224317 mRNA. Translation: AAF61097.1.
IPI	IPI00585261.
RefSeq	NP_989552.1. NM_204221.1.
UniGene	Gga.110.
3D structure databases
ProteinModelPortal	O57329.
SMR	O57329. Positions 45-162.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Proteomic databases
PaxDb	O57329.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	374060.
KEGG	gga:374060.
Organism-specific databases
CTD	8324.
Phylogenomic databases
eggNOG	NOG257258.
HOGENOM	HOG000233237.
HOVERGEN	HBG006977.
InParanoid	O57329.
KO	K02432.
OrthoDB	EOG4V437C.
Family and domain databases
Gene3D	1.10.2000.10. 1 hit.
InterPro	IPR000539. Frizzled. IPR015526. Frizzled/SFRP. IPR020067. Frizzled_dom. IPR026552. FZD7. IPR017981. GPCR_2-like. [Graphical view]
PANTHER	PTHR11309. PTHR11309. 1 hit. PTHR11309:SF31. PTHR11309:SF31. 1 hit.
Pfam	PF01534. Frizzled. 1 hit. PF01392. Fz. 1 hit. [Graphical view]
PRINTS	PR00489. FRIZZLED.
SMART	SM00063. FRI. 1 hit. [Graphical view]
SUPFAM	SSF63501. Frizzled_Cys-rich. 1 hit.
PROSITE	PS50038. FZ. 1 hit. PS50261. G_PROTEIN_RECEP_F2_4. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20813582.

Entry information

Entry name

FZD7_CHICK

Accession

Primary (citable) accession number: O57329
Secondary accession number(s): Q9IA04

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 5, 2001
Last sequence update:	June 1, 1998
Last modified:	April 3, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program