O57314 (DHB12_ANAPL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Estradiol 17-beta-dehydrogenase 12
EC=1.1.1.62

Alternative name(s):
17-beta-hydroxysteroid dehydrogenase 12
Short name=17-beta-HSD 12

Gene names

Name:	HSD17B12
Synonyms:	SPM2

Organism

Anas platyrhynchos (Domestic duck) (Anas boschas)

Taxonomic identifier

8839 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Anseriformes › Anatidae › Anas

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the transformation of estrone (E1) into estradiol (E2), suggesting a central role in estrogen formation By similarity.
Catalytic activity	Estradiol-17-beta + NAD(P)+ = estrone + NAD(P)H.
Pathway	Steroid biosynthesis; estrogen biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Tissue specificity	Brain.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	estradiol 17-beta-dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Estradiol 17-beta-dehydrogenase 12		PRO_0000054577
Regions
	Transmembrane	33 – 53	21	Helical; Potential
	Transmembrane	181 – 201	21	Helical; Potential
	Transmembrane	274 – 294	21	Helical; Potential
	Nucleotide binding	48 – 77	30	NADP By similarity
Sites
	Active site	201	1	Proton acceptor By similarity
	Binding site	188	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O57314 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 744D8F44491A9262	FASTA	312	33,896
	10 20 30 40 50 60 MLPAAGLLWW VGALGALYAA VRGALGLLGA LRVWGIGAGR AALGPGLGAW AVVTGATDGI 70 80 90 100 110 120 GKAYAKELAK RGMKVALISR SKEKLDQVAG EITEQYGVET KVIVADFGER EDIYDRIRAG 130 140 150 160 170 180 LEGLEIGVLV NNVGISYSYP EYFIDVPDLD KTIDKMININ IMSVCKMTRL VLPGMLERSK 190 200 210 220 230 240 GVILNISSAA GMYPTPLLTL YSASKAFVDY FSRGLHAEYK SKGIIVQSVM PYYVATKMSK 250 260 270 280 290 300 ISKPSFDKPT PETYVRAAIG TVGLQSQTNG CLPHAFMGWV FSILPTSTVM NLLMKTNKQI 310 RARFLKKKMK EK

References

[1]	"Molecular cloning of SPM2 from duckling brain: a putative brain specific steroid dehydrogenase and its induction by imprinting stimuli." Kimura N., Kurosawa N., Kondo K., Tsukada Y. Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Forebrain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB009304 mRNA. Translation: BAA23765.1.
3D structure databases
ProteinModelPortal	O57314.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG005478.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHB12_ANAPL

Accession

Primary (citable) accession number: O57314

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	December 14, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program