O57250 (DNLI_VACCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA ligase
EC=6.5.1.1

Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:	LIG
Ordered Locus Names:	MVA163R, ACAM3000_MVA_163

Organism

Vaccinia virus (strain Ankara) (VACV)

Taxonomic identifier

126794 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus › Vaccinia virus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	552 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. It is not essential for viral replication and recombination.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Cofactor	Divalent metal cations By similarity.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Metal-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	DNA ligation involved in DNA repair Inferred from electronic annotation. Source: InterPro DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 552	552	DNA ligase		PRO_0000059587
Sites
	Active site	231	1	N6-AMP-lysine intermediate By similarity
	Metal binding	283	1	Divalent metal cation 1 By similarity
	Metal binding	377	1	Divalent metal cation 2 By similarity
	Binding site	229	1	ATP By similarity
	Binding site	236	1	ATP By similarity
	Binding site	251	1	ATP By similarity
	Binding site	283	1	ATP By similarity
	Binding site	317	1	ATP By similarity
	Binding site	393	1	ATP By similarity
	Binding site	397	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O57250 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: AC7E18C59F4C00C1	FASTA	552	63,468
	10 20 30 40 50 60 MTSLREFRKL CCDIYHASGY KEKSKLIRDF ITDRDDKYLI IKLLLPGLDD RIYNMNDKQI 70 80 90 100 110 120 IKLYSIIFKQ SQEDMLQDLG YGYIGDTIRT FFKENTEIRP RDKSILTLEE VDSFLTTLSS 130 140 150 160 170 180 VTKESHQIKL LTDIASVCTC NDLKCVVMLI DKDLKIKAGP RYVLNAISPH AYDVFRKSNN 190 200 210 220 230 240 LKEIIENASK QNLDSISISV MTPINPMLAE SCDSVNKAFK KFPSGMFAEV KYDGERVQVH 250 260 270 280 290 300 KNNNEFAFFS RNMKPVLSHK VDYLKEYIPK AFKKATSIVL DSEIVLVDEH NVPLPFGSLG 310 320 330 340 350 360 IHKKKEYKNS NMCLFVFDCL YFDGFDMTDI PLYERRSFLK DVMVEIPNRI VFSELTNISN 370 380 390 400 410 420 ESQLTDVLDD ALTRKLEGLV LKDINGVYEP GKRRWLKIKR DYLNEGSMAD SADLVVLGAY 430 440 450 460 470 480 YGKGAKGGIM AVFLMGCYDD ESGKWKTVTK CSGHDDNTLR ELQDQLKMIK INKDPKKIPE 490 500 510 520 530 540 WLVVNKIYIP DFVVEDPKQS QIWEISGAEF TSSKSHTANG ISIRFPRFTR IREDKTWKES 550 THLNDLVNLT KS

References

[1]	"The complete genomic sequence of the modified vaccinia Ankara strain: comparison with other orthopoxviruses." Antoine G., Scheiflinger F., Dorner F., Falkner F.G. Virology 244:365-396(1998) [PubMed: 9601507] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	Esposito J.J., Frace M., Sammons S.A., Olsen-Rasmussen M.S., Osborne J., Khristova M., Wohlhueter R.M. Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Isolate Acambis 3000.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U94848 Genomic DNA. Translation: AAB96541.1. AY603355 Genomic DNA. Translation: AAT10561.1.
PIR	T37436.
3D structure databases
ProteinModelPortal	O57250.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000977. DNA_ligase_ATP-dep. IPR012309. DNA_ligase_ATP-dep_C. IPR012310. DNA_ligase_ATP-dep_cent. IPR016059. DNA_ligase_ATP-dep_CS. IPR012308. DNA_ligase_ATP-dep_N. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00574. Dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_VACCA

Accession

Primary (citable) accession number: O57250

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	June 1, 1998
Last modified:	September 21, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program