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Reviewed, UniProtKB/Swiss-Prot O57250 (DNLI_VACCA)

Last modified November 25, 2008. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    DNA ligase
    EC=6.5.1.1
Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP]
Gene names
Name: LIG
Ordered Locus Names: MVA163R, ACAM3000_MVA_163
OrganismVaccinia virus (strain Ankara) (VACV)
Taxonomic identifier126794 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length552 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

General annotation (Comments)

Function

This protein seals, during DNA replication, DNA recombination and DNA repair, nicks in double-stranded DNA. It is not essential for viral replication and recombination.

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

Sequence similarities

Belongs to the ATP-dependent DNA ligase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 552552DNA ligase
PRO_0000059587

Sites

Active site2311N6-AMP-lysine intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
O57250-1 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: AC7E18C59F4C00C1

FASTA55263,468
        10         20         30         40         50         60 
MTSLREFRKL CCDIYHASGY KEKSKLIRDF ITDRDDKYLI IKLLLPGLDD RIYNMNDKQI 

        70         80         90        100        110        120 
IKLYSIIFKQ SQEDMLQDLG YGYIGDTIRT FFKENTEIRP RDKSILTLEE VDSFLTTLSS 

       130        140        150        160        170        180 
VTKESHQIKL LTDIASVCTC NDLKCVVMLI DKDLKIKAGP RYVLNAISPH AYDVFRKSNN 

       190        200        210        220        230        240 
LKEIIENASK QNLDSISISV MTPINPMLAE SCDSVNKAFK KFPSGMFAEV KYDGERVQVH 

       250        260        270        280        290        300 
KNNNEFAFFS RNMKPVLSHK VDYLKEYIPK AFKKATSIVL DSEIVLVDEH NVPLPFGSLG 

       310        320        330        340        350        360 
IHKKKEYKNS NMCLFVFDCL YFDGFDMTDI PLYERRSFLK DVMVEIPNRI VFSELTNISN 

       370        380        390        400        410        420 
ESQLTDVLDD ALTRKLEGLV LKDINGVYEP GKRRWLKIKR DYLNEGSMAD SADLVVLGAY 

       430        440        450        460        470        480 
YGKGAKGGIM AVFLMGCYDD ESGKWKTVTK CSGHDDNTLR ELQDQLKMIK INKDPKKIPE 

       490        500        510        520        530        540 
WLVVNKIYIP DFVVEDPKQS QIWEISGAEF TSSKSHTANG ISIRFPRFTR IREDKTWKES 

       550 
THLNDLVNLT KS 

« Hide

References

[1]"The complete genomic sequence of the modified vaccinia Ankara strain: comparison with other orthopoxviruses."
Antoine G., Scheiflinger F., Dorner F., Falkner F.G.
Virology 244:365-396(1998) [PubMed: 9601507] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]Esposito J.J., Frace M., Sammons S.A., Olsen-Rasmussen M.S., Osborne J., Khristova M., Wohlhueter R.M.
Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Isolate Acambis 3000.

Cross-references

Sequence databases

U94848 Genomic DNA. Translation: AAB96541.1.
AY603355 Genomic DNA. Translation: AAT10561.1.
PIRT37436.

3D structure databases

ModBaseSearch...

Family and domain databases

InterProIPR000977. DNA_ligase.
IPR012309. DNA_ligase_A_C.
IPR012310. DNA_ligase_A_M.
IPR012308. DNA_ligase_A_N.
IPR016059. DNA_ligase_CS.
IPR012340. NA-bd_OB-fold.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLI_VACCA
AccessionPrimary (citable) accession number: O57250
Entry history
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: June 1, 1998
Last modified: November 25, 2008
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectVirus (Virus annotation project)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents