Reviewed, UniProtKB/Swiss-Prot O57245 (3BHS_VACCA)

Names and origin

Protein names

Recommended name:
    3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase
      Short name=3-beta-HSD
Including the following 2 domains:
    1- Recommended name:
            3-beta-hydroxy-Delta(5)-steroid dehydrogenase
              EC=1.1.1.145
        Alternative name(s):
            3-beta-hydroxy-5-ene steroid dehydrogenase
            Progesterone reductase
    2- Recommended name:
            Steroid Delta-isomerase
              EC=5.3.3.1
        Alternative name(s):
            Delta-5-3-ketosteroid isomerase

Gene names

Ordered Locus Names:

MVA157L, ACAM3000_MVA_157

Organism

Vaccinia virus (strain Ankara) (VACV)

Taxonomic identifier

126794 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus › Vaccinia virus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.
Catalytic activity	A 3-beta-hydroxy-Delta(5)-steroid + NAD+ = a 3-oxo-Delta(5)-steroid + NADH. A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid.
Pathway	Lipid metabolism; steroid biosynthesis.
Sequence similarities	Belongs to the 3-beta-HSD family.

Ontologies

Keywords
Biological process	Steroidogenesis
Ligand	NAD
Molecular function	Isomerase Oxidoreductase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-beta-hydroxy-delta5-steroid dehydrogenase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro steroid delta-isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase		PRO_0000087793
Sites
	Active site	147	1	Proton acceptor By similarity
	Binding site	151	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	O57245-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 8A5F09E596B98C7E	FASTA	346	39,397
	10 20 30 40 50 60 MAVYAVTGGA GFLGRYIVKL LISADDVQEI RVIDIVEDPQ PITSKVKVIN YIQCDINDFD 70 80 90 100 110 120 KVREALDGVN LIIHTAALVD VFGKYTDNEI MKVNYYGTQT ILAACVDLGI KYLIYTSSME 130 140 150 160 170 180 AIGPNKHGDP FIGHEHTLYD ISPGHVYAKS KRMAEQLVMK ANNSVIMNGA KLYTCCLRPT 190 200 210 220 230 240 GIYGEGDKLM KVFYEQCKQH GNIMYRTVDD NAVHSRVYVG NAAWMHVLAA KYIQYPGSEI 250 260 270 280 290 300 KGNAYFCYDY SPSCSYDMFN LLLMKPLGIE QGSRIPRWML KMYACKNDMK RILFRKPSLL 310 320 330 340 NNYTLKISNT TFEVRTNNAE LDFNYSPIFN VDVAFERTRK WLEESE

References

[1]	"The complete genomic sequence of the modified vaccinia Ankara strain: comparison with other orthopoxviruses." Antoine G., Scheiflinger F., Dorner F., Falkner F.G. Virology 244:365-396(1998) [PubMed: 9601507] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	Esposito J.J., Frace M., Sammons S.A., Olsen-Rasmussen M.S., Osborne J., Khristova M., Wohlhueter R.M. Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Isolate Acambis 3000.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U94848 Genomic DNA. Translation: AAB96479.1. AY603355 Genomic DNA. Translation: AAT10555.1.
PIR	T37430.
3D structure databases
SMR	O57245. Positions 4-345.
ModBase	Search...
Family and domain databases
InterPro	IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

3BHS_VACCA

Accession

Primary (citable) accession number: O57245

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	June 1, 1998
Last modified:	February 9, 2010
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)