Reviewed, UniProtKB/Swiss-Prot O57209 (MCEL_VACCA)

Names and origin

Protein names

Recommended name:
    mRNA-capping enzyme large subunit
Including the following 2 domains:
    1- Recommended name:
            Polynucleotide 5'-triphosphatase
              EC=3.1.3.33
        Alternative name(s):
            mRNA 5'-triphosphatase
              Short name=TPase
    2- Recommended name:
            mRNA guanylyltransferase
              EC=2.7.7.50
        Alternative name(s):
            GTP--RNA guanylyltransferase
              Short name=GTase

Gene names

Ordered Locus Names:

MVA098R, ACAM3000_MVA_098

Organism

Vaccinia virus (strain Ankara) (VACV)

Taxonomic identifier

126794 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	844 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the first two reactions in the mRNA cap formation pathway.
Catalytic activity	A 5'-phosphopolynucleotide + H(2)O = a polynucleotide + phosphate. GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
Subunit structure	Heterodimer of a large and a small subunit.
Sequence similarities	Belongs to the viral GTase family.

Ontologies

Keywords
Biological process	mRNA capping mRNA processing
Molecular function	Hydrolase Nucleotidyltransferase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: InterPro
Molecular function	mRNA guanylyltransferase activity Inferred from electronic annotation. Source: EC polynucleotide 5'-phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 844	844	mRNA-capping enzyme large subunit		PRO_0000210121
Sites
	Active site	260	1	N6-GMP-lysine intermediate Potential

Sequences

	Sequence		Length	Mass (Da)
	O57209-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: BC7052355070FE2C	FASTA	844	96,761
	10 20 30 40 50 60 MDANIVSSST IATYIDALAK NASELEQRST AYEINNELEL VFIKPPLITL TNVVNISTIQ 70 80 90 100 110 120 ESFIRFTVTN KEGVKIRTKI PLSKVHGLDV KNVQLVDAID NIVWEKKSLV TENRLHKECL 130 140 150 160 170 180 LRLSTEERHI FLDYKKYGSS IRLELVNLIQ AKTKNFTIDF KLKYFLGSGA QSKSSLLHAI 190 200 210 220 230 240 NHPKSRPNTS LEIEFTPRDN EKVPYDELIK ELTTLSRHIF MASPENVILS PPINAPIKTF 250 260 270 280 290 300 MLPKQDIVGL DLENLYAVTK TDGIPITIRV TSKGLYCYFT HLGYIIRYPV KRIIDSEVVV 310 320 330 340 350 360 FGEAVKDKNW TVYLIKLIEP VNAINDRLEE SKYVESKLVD ICDRIVFKSK KYEGPFTTTS 370 380 390 400 410 420 EVVDMLSTYL PKQPEGVILF YSKGPKSNID FKIKKENTID QTANVVFRYM SSEPIIFGES 430 440 450 460 470 480 SIFVEYKKFS NDKGFPKEYG SGKIVLYNGV NYLNNIYCLE YINTHNEVGI KSVVVPIKFI 490 500 510 520 530 540 AEFLVNGEIL KPRIDKTMKY INSEDYYGNQ HNIIVEHLRD QSIKIGDIFN EDKLSDVGHQ 550 560 570 580 590 600 YANNDKFRLN PEVSYFTNKR TRGPLGILSN YVKTLLISMY CSKTFLDDSN KRKVLAIDFG 610 620 630 640 650 660 NGADLEKYFY GEIALLVATD PDADAIARGN ERYNKLNSGI KTKYYKFDYI QETIRSDTFV 670 680 690 700 710 720 SSVREVFYFG KFNIIDWQFA IHYSFHPRHY ATVMNNLSEL TASGGKVLIT TMDGDKLSKL 730 740 750 760 770 780 TDKKTFIIHK NLPSSENYMS VEKIADDRIV VYNPSTMSTP MTEYIIKKND IVRVFNEYGF 790 800 810 820 830 840 VLVDNVDFAT IIERSKKFIN GASTMEDRPS TKNFFELNRG AIKCEGLDVE DLLSYYVVYV FSKR

References

[1]	"The complete genomic sequence of the modified vaccinia Ankara strain: comparison with other orthopoxviruses." Antoine G., Scheiflinger F., Dorner F., Falkner F.G. Virology 244:365-396(1998) [PubMed: 9601507] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	Esposito J.J., Frace M., Sammons S.A., Olsen-Rasmussen M.S., Osborne J., Khristova M., Wohlhueter R.M. Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Isolate Acambis 3000.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U94848 Genomic DNA. Translation: AAB96511.1. AY603355 Genomic DNA. Translation: AAT10496.1.
PIR	T37374.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR004971. Pox_MCEL. [Graphical view]
Pfam	PF03291. Pox_MCEL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MCEL_VACCA

Accession

Primary (citable) accession number: O57209

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	June 1, 1998
Last modified:	November 25, 2008
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)