ID   RPO1_VACCA              Reviewed;        1286 AA.
AC   O57204;
DT   07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   16-JUN-2009, entry version 36.
DE   RecName: Full=DNA-directed RNA polymerase 147 kDa polypeptide;
DE            EC=2.7.7.6;
GN   Name=RPO147; OrderedLocusNames=MVA090R, ACAM3000_MVA_090;
OS   Vaccinia virus (strain Ankara) (VACV).
OC   Viruses; dsDNA viruses, no RNA stage; Poxviridae; Chordopoxvirinae;
OC   Orthopoxvirus.
OX   NCBI_TaxID=126794;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   MEDLINE=98263813; PubMed=9601507; DOI=10.1006/viro.1998.9123;
RA   Antoine G., Scheiflinger F., Dorner F., Falkner F.G.;
RT   "The complete genomic sequence of the modified vaccinia Ankara strain:
RT   comparison with other orthopoxviruses.";
RL   Virology 244:365-396(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Isolate Acambis 3000;
RA   Esposito J.J., Frace M., Sammons S.A., Olsen-Rasmussen M.S.,
RA   Osborne J., Khristova M., Wohlhueter R.M.;
RL   Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription
CC       of DNA into RNA using the four ribonucleoside triphosphates as
CC       substrates.
CC   -!- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate
CC       + RNA(n+1).
CC   -!- SUBUNIT: This enzyme consists of at least eight subunits.
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DR   EMBL; U94848; AAB96506.1; -; Genomic_DNA.
DR   EMBL; AY603355; AAT10488.1; -; Genomic_DNA.
DR   PIR; T37366; T37366.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006350; P:transcription; IEA:UniProtKB-KW.
DR   InterPro; IPR000722; RNA_pol_asu.
DR   InterPro; IPR006592; RNA_pol_N.
DR   InterPro; IPR007066; RNA_pol_Rpb1_3.
DR   InterPro; IPR007083; RNA_pol_Rpb1_4.
DR   Gene3D; G3DSA:2.40.40.30; RNA_pol_A; 1.
DR   Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR   Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR   Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR   SMART; SM00663; RPOLA_N; 1.
PE   4: Predicted;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW   Transferase.
FT   CHAIN         1   1286       DNA-directed RNA polymerase 147 kDa
FT                                polypeptide.
FT                                /FTId=PRO_0000073918.
SQ   SEQUENCE   1286 AA;  146886 MW;  EE51E65E4BBCA2D7 CRC64;
     MAVISKVTYS LYDQKEINAT DIIISHVKND DDIGTVKDGR LGAMDGALCK TCGKTELECF
     GHWGKVSIYK THIVKPEFIS EIIRLLNYIC IHCGLLRSRE PYSDDINLKE LSGHALRRLK
     DKILSKKKSC WNSECMQPYQ KITFSKKKVC FVNKLDDINV PNSLIYQKLI SIHEKFWPLL
     EIHQYPANLF YTDYFPIPPL IIRPAISFWI DSIPKETNEL TYLLGMIVKN CNLNADEQVI
     QKAVIEYDDI KIISNNTSSI NLSYITSGKN NMIRSYIVAR RKDQTARSVI GPSTSITVNE
     VGMPAYIRNT LTEKIFVNAF TVDKVKQLLA SNQVKFYFNK RLNQLTRIRQ GKFIKNKIHL
     LPGDWVEVAV QEYTSIIFGR QPSLHRYNVI ASSIRATEGD TIKISPGIVN SQNADFDGDE
     EWMILEQNPK AVIEQSILMY PTTLLKHDIH GAPVYGSIQD EIVAAYSLFR IQDLCLDEVL
     NILGKYGREF DPKGKCKFSG KDIYTYLIGE KINYPGLLKD GEIIANDVDS NFVVAMRHLS
     LAGLLSDHKS NVEGINFIIK SSYVFKRYLS IYGFGVTFKD LRPNSTFTNK LEAINVEKIE
     LIKEAYAKYL NDVRDGKIVP LSKALEADYV ESMLSNLTNL NIREIEEHMR QTLIDDPDNN
     LLKMAKAGYK VNPTELMYIL GTYGQQRIDG EPAETRVLGR VLPYYLPDSK DPEGRGYILN
     SLTKGLTGSQ YYFSMLVARS QSTDIVCETS RTGTLARKII KKMEDMVVDG YGQVVIGNTL
     IKYAANYTKI LGSVCKPVDL IYPDESMTWY LEISALWNKI KQGFVYSQKQ KLAKKTLAPF
     NFLVFVKPTT EDNAIKVKDL YDMIHNVIDD VREKYFFTVS NIDFMEYIFL THLNPSRIRI
     TKETAITIFE KFYEKLNYTL GGGTPIGIIS AQVLSEKFTQ QALSSFHTTE KSGAVKQKLG
     FNEFNNLTNL SKNKTEIITL VSDDISKLQS VKINFEFVCL GELNPNITLR KETDRYVVDI
     IVNRLYIKRA EITELVVEYM IERFISFSVI VKEWGMETFI EDEDNIRFTV YLNFVEPEEL
     NLSKFMMVLP GAANKGKISK FKIPISDYTG YDDFNQTKKL NKMTVELMNL KELGSFDLEN
     VNVYPGVWNT YDIFGIEAAR EYLCEAMLNT YGEGFDYLYQ PCDLLASLLC ASYEPESVNK
     FKFGAASTLK RATFGDNKAL LNAALHKKSE PINDNSSCHF FSKVPNIGTG YYKYFIDLGL
     LMRMERKLSD KISSQKIKEM EETEDF
//