O57204 (RP147_VACCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase 147 kDa polypeptide
EC=2.7.7.6

Gene names

Name:	RPO147
Ordered Locus Names:	MVA090R, ACAM3000_MVA_090

Organism

Vaccinia virus (strain Ankara) (VACV)

Taxonomic identifier

126794 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus › Vaccinia virus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	1286 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of the DNA-dependent RNA polymerase which catalyzes the transcription of viral DNA into RNA using the four ribonucleoside triphosphates as substrates. Responsible for the transcription of early, intermediate and late genes. DNA-dependent RNA polymerase associates with the early transcription factor (ETF), itself composed of D6 and A7, thereby allowing the early genes transcription. Late transcription, and probably also intermediate transcription, require newly synthesized RNA polymerase.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	The DNA-dependent RNA polymerase used for intermediate and late genes expression consists of eight subunits 147kDa, 133kDa, 35kDa, 30kDa, 22kDa, 19kDa, 18kDa and 7kDa totalling more than 500 kDa in mass. The same holoenzyme, with the addition of the transcription-specificity factor RAP94, is used for early gene expression.
Subcellular location	Virion Probable. Note: All the enzymes and other proteins required to synthesize early mRNAs are packaged within the virion core along with the DNA genome. This is necessary because viral early mRNAs are synthesized within minutes after virus entry into the cell and are extruded through pores in the core particle.
Sequence similarities	Belongs to the poxviridae DNA-directed RNA polymerase 147 kDa subunit family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase Virion
Molecular function	Nucleotidyltransferase Transferase
Gene Ontology (GO)
Cellular component	virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1286	1286	DNA-directed RNA polymerase 147 kDa polypeptide		PRO_0000073918

Sequences

	Sequence		Length	Mass (Da)
	O57204 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: EE51E65E4BBCA2D7	FASTA	1,286	146,886
	10 20 30 40 50 60 MAVISKVTYS LYDQKEINAT DIIISHVKND DDIGTVKDGR LGAMDGALCK TCGKTELECF 70 80 90 100 110 120 GHWGKVSIYK THIVKPEFIS EIIRLLNYIC IHCGLLRSRE PYSDDINLKE LSGHALRRLK 130 140 150 160 170 180 DKILSKKKSC WNSECMQPYQ KITFSKKKVC FVNKLDDINV PNSLIYQKLI SIHEKFWPLL 190 200 210 220 230 240 EIHQYPANLF YTDYFPIPPL IIRPAISFWI DSIPKETNEL TYLLGMIVKN CNLNADEQVI 250 260 270 280 290 300 QKAVIEYDDI KIISNNTSSI NLSYITSGKN NMIRSYIVAR RKDQTARSVI GPSTSITVNE 310 320 330 340 350 360 VGMPAYIRNT LTEKIFVNAF TVDKVKQLLA SNQVKFYFNK RLNQLTRIRQ GKFIKNKIHL 370 380 390 400 410 420 LPGDWVEVAV QEYTSIIFGR QPSLHRYNVI ASSIRATEGD TIKISPGIVN SQNADFDGDE 430 440 450 460 470 480 EWMILEQNPK AVIEQSILMY PTTLLKHDIH GAPVYGSIQD EIVAAYSLFR IQDLCLDEVL 490 500 510 520 530 540 NILGKYGREF DPKGKCKFSG KDIYTYLIGE KINYPGLLKD GEIIANDVDS NFVVAMRHLS 550 560 570 580 590 600 LAGLLSDHKS NVEGINFIIK SSYVFKRYLS IYGFGVTFKD LRPNSTFTNK LEAINVEKIE 610 620 630 640 650 660 LIKEAYAKYL NDVRDGKIVP LSKALEADYV ESMLSNLTNL NIREIEEHMR QTLIDDPDNN 670 680 690 700 710 720 LLKMAKAGYK VNPTELMYIL GTYGQQRIDG EPAETRVLGR VLPYYLPDSK DPEGRGYILN 730 740 750 760 770 780 SLTKGLTGSQ YYFSMLVARS QSTDIVCETS RTGTLARKII KKMEDMVVDG YGQVVIGNTL 790 800 810 820 830 840 IKYAANYTKI LGSVCKPVDL IYPDESMTWY LEISALWNKI KQGFVYSQKQ KLAKKTLAPF 850 860 870 880 890 900 NFLVFVKPTT EDNAIKVKDL YDMIHNVIDD VREKYFFTVS NIDFMEYIFL THLNPSRIRI 910 920 930 940 950 960 TKETAITIFE KFYEKLNYTL GGGTPIGIIS AQVLSEKFTQ QALSSFHTTE KSGAVKQKLG 970 980 990 1000 1010 1020 FNEFNNLTNL SKNKTEIITL VSDDISKLQS VKINFEFVCL GELNPNITLR KETDRYVVDI 1030 1040 1050 1060 1070 1080 IVNRLYIKRA EITELVVEYM IERFISFSVI VKEWGMETFI EDEDNIRFTV YLNFVEPEEL 1090 1100 1110 1120 1130 1140 NLSKFMMVLP GAANKGKISK FKIPISDYTG YDDFNQTKKL NKMTVELMNL KELGSFDLEN 1150 1160 1170 1180 1190 1200 VNVYPGVWNT YDIFGIEAAR EYLCEAMLNT YGEGFDYLYQ PCDLLASLLC ASYEPESVNK 1210 1220 1230 1240 1250 1260 FKFGAASTLK RATFGDNKAL LNAALHKKSE PINDNSSCHF FSKVPNIGTG YYKYFIDLGL 1270 1280 LMRMERKLSD KISSQKIKEM EETEDF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genomic sequence of the modified vaccinia Ankara strain: comparison with other orthopoxviruses." Antoine G., Scheiflinger F., Dorner F., Falkner F.G. Virology 244:365-396(1998) [PubMed: 9601507] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	Esposito J.J., Frace M., Sammons S.A., Olsen-Rasmussen M.S., Osborne J., Khristova M., Wohlhueter R.M. Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Isolate Acambis 3000.
[3]	"Vaccinia virus transcription." Broyles S.S. J. Gen. Virol. 84:2293-2303(2003) [PubMed: 12917449] [Abstract] Cited for: REVIEW.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U94848 Genomic DNA. Translation: AAB96506.1. AY603355 Genomic DNA. Translation: AAT10488.1.
PIR	T37366.
3D structure databases
ProteinModelPortal	O57204.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000722. RNA_pol_asu. IPR006592. RNA_pol_N. IPR007080. RNA_pol_Rpb1_1. IPR007066. RNA_pol_Rpb1_3. IPR007083. RNA_pol_Rpb1_4. IPR007081. RNA_pol_Rpb1_5. [Graphical view]
Pfam	PF04997. RNA_pol_Rpb1_1. 1 hit. PF00623. RNA_pol_Rpb1_2. 1 hit. PF04983. RNA_pol_Rpb1_3. 1 hit. PF05000. RNA_pol_Rpb1_4. 1 hit. PF04998. RNA_pol_Rpb1_5. 1 hit. [Graphical view]
SMART	SM00663. RPOLA_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RP147_VACCA

Accession

Primary (citable) accession number: O57204

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	June 1, 1998
Last modified:	November 16, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program